☆ 4.7 Article

A Refined Model of the Prototypical Salmonella SPI-1 T3SS Basal Body Reveals the Molecular Basis for Its Assembly

PLOS PATHOGENS (2013)

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Journal

PLOS PATHOGENS
Volume 9, Issue 4, Pages -

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PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.ppat.1003307

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Categories

Microbiology Parasitology Virology

Funding

  1. Canadian Institute of Health Research
  2. HHMI International Scholar Program
  3. National Center for Research Resources [P41 RR11823]
  4. National Institute of General Medical Studies from the National Institutes of Health [P41 GM103533]
  5. Canadian Foundation of Innovation
  6. BCKDF

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The T3SS injectisome is a syringe-shaped macromolecular assembly found in pathogenic Gram-negative bacteria that allows for the direct delivery of virulence effectors into host cells. It is composed of a basal body'', a lock-nut structure spanning both bacterial membranes, and a needle'' that protrudes away from the bacterial surface. A hollow channel spans throughout the apparatus, permitting the translocation of effector proteins from the bacterial cytosol to the host plasma membrane. The basal body is composed largely of three membrane-embedded proteins that form oligomerized concentric rings. Here, we report the crystal structures of three domains of the prototypical Salmonella SPI-1 basal body, and use a new approach incorporating symmetric flexible backbone docking and EM data to produce a model for their oligomeric assembly. The obtained models, validated by biochemical and in vivo assays, reveal the molecular details of the interactions driving basal body assembly, and notably demonstrate a conserved oligomerization mechanism.

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