Article
Multidisciplinary Sciences
Yuya Nishida, Sachiko Yanagisawa, Rikuri Morita, Hideki Shigematsu, Kyoko Shinzawa-Itoh, Hitomi Yuki, Satoshi Ogasawara, Ken Shimuta, Takashi Iwamoto, Chisa Nakabayashi, Waka Matsumura, Hisakazu Kato, Chai Gopalasingam, Takemasa Nagao, Tasneem Qaqorh, Yusuke Takahashi, Satoru Yamazaki, Katsumasa Kamiya, Ryuhei Harada, Nobuhiro Mizuno, Hideyuki Takahashi, Yukihiro Akeda, Makoto Ohnishi, Yoshikazu Ishii, Takashi Kumasaka, Takeshi Murata, Kazumasa Muramoto, Takehiko Tosha, Yoshitsugu Shiro, Teruki Honma, Yasuteru Shigeta, Minoru Kubo, Seiji Takashima, Yasunori Shintani
Summary: Antimicrobial resistance is a global health problem and there is a rise in drug-resistant species. Our research has identified a new antibiotic compound that can combat drug-resistant Neisseria gonorrhoeae, and our findings on the inhibitory mechanism provide new avenues for modulating protein functions and overcoming antimicrobial resistance.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Jia Shi, Yi Yang, Ya-Nan Wang, Qing Li, Xue Xing, An-Ying Cheng, Xiao-Na Zhan, Jie Li, Gang Xu, Fan He
Summary: This study reveals the association between metabolism and the progression of vascular calcification in chronic kidney disease (CKD). High phosphate and uremic serum enhance mitochondrial respiration capacity in vascular smooth muscle cells (VSMCs), while glycolysis level remains unchanged. Suppression of cytochrome c oxidase (COX) activity attenuates vascular calcification.
CELL DEATH & DISEASE
(2022)
Article
Multidisciplinary Sciences
Gizem Inak, Agnieszka Rybak-Wolf, Pawel Lisowski, Tancredi M. Pentimalli, Rene Juettner, Petar Glazar, Karan Uppal, Emanuela Bottani, Dario Brunetti, Christopher Secker, Annika Zink, David Meierhofer, Marie-Therese Henke, Monishita Dey, Ummi Ciptasari, Barbara Mlody, Tobias Hahn, Maria Berruezo-Llacuna, Nikos Karaiskos, Michela Di Virgilio, Johannes A. Mayr, Saskia B. Wortmann, Josef Priller, Michael Gotthardt, Dean P. Jones, Ertan Mayatepek, Werner Stenzel, Sebastian Diecke, Ralf Kuehn, Erich E. Wanker, Nikolaus Rajewsky, Markus Schuelke, Alessandro Prigione
Summary: Leigh syndrome (LS) is a severe neurometabolic disorder lacking effective models. This study found that LS carrying mutations in SURF1 gene exhibit impaired neuronal morphogenesis due to metabolic deficiencies.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Yijuan Ding, Yangui Chen, Zhaohui Wu, Nan Yang, Kusum Rana, Xiao Meng, Bangyan Liu, Huafang Wan, Wei Qian
Summary: This study functionally characterized the role of Cox17 in the pathogenesis of Sclerotinia sclerotiorum and found that it is positively associated with virulence and oxidative detoxification.
Article
Multidisciplinary Sciences
Michael B. Powner, Glen Jeffery
Summary: Systemic glucose levels can be modulated through specific solar wavelengths that affect mitochondrial metabolism. Different wavelengths of light have opposing effects on mitochondrial respiration, with longer wavelengths increasing ATP production and shorter wavelengths suppressing metabolism. This study in a bumblebee model found that manipulating mitochondrial activity with specific wavelengths of light significantly altered circulating glucose concentration. This research contributes to our understanding of how light can affect systemic metabolism.
Article
Cell Biology
Sue Ellen Brand, Martha Scharlau, Lois Geren, Marissa Hendrix, Clayre Parson, Tyler Elmendorf, Earl Neel, Kaila Pianalto, Jennifer Silva-Nash, Bill Durham, Francis Millett
Summary: Cytochrome c (Cc) has undergone accelerated evolution from anthropoid primates to humans, with several amino acid changes occurring near the binding site of the Cc:CcO complex. These changes affect the dissociation constant and the role of phosphorylation in regulating mitochondrial electron transport and membrane potential.
Article
Multidisciplinary Sciences
Agnes Moe, Justin Di Trani, John L. Rubinstein, Peter Brzezinski
Summary: Energy conversion in aerobic organisms involves electron transfer from low-potential donors to dioxygen through the membrane-bound respiratory chain. The formation of supercomplex CIII2CIV1/2 facilitates electron transfer by two-dimensional diffusion of cyt. c, increasing QH(2):O-2 oxidoreductase activity and suggesting a regulatory role for supercomplex formation in the respiratory chain.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Clinical Neurology
Ana Martin-Sanchez, Hector Gonzalez-Pardo, Laia Alegre-Zurano, Adriana Castro-Zavala, Isabel Lopez-Taboada, Olga Valverde, Nelida M. Conejo
Summary: Gender is considered a key determinant of mental health, with psychiatric disorders such as anxiety and depression more common in women. Impaired brain energy metabolism has been identified as a risk factor for the development of these disorders. However, comprehensive studies on the behavioral and neurobiological effects in this context are limited. This study explores the effects of cannabidiol on anxiety and depression in maternally separated female mice, finding that cannabidiol could alleviate these behaviors but not restore brain energy metabolism impairment.
PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY
(2022)
Article
Biochemistry & Molecular Biology
Yu-De Chu, Siew-Na Lim, Chau-Ting Yeh, Wey-Ran Lin
Summary: COX5B is a potential biomarker associated with unfavorable prognosis in colorectal cancers (CRCs). It promotes cell growth and attenuates drug susceptibility in CRCs cells through modulating CLDN2 expression.
Article
Chemistry, Multidisciplinary
Wenshu Cong, Li Meng, Yuanjie Pan, Han Wang, Jiahui Zhu, Yuanyu Huang, Qingli Huang
Summary: Population aging is a global issue, and this study presents a novel approach using Pd-based single-atom nanozymes to improve mitochondrial function and attenuate aging. The nanozyme mimics enzyme activities in the mitochondrial respiratory chain and enhances mitochondrial activities and abundance. Additionally, it demonstrates excellent anti-aging and neuroprotective effects through the activation of the mitochondrial unfolded protein response (UPRmt) pathway.
Article
Chemistry, Physical
Margareta R. A. Blomberg
Summary: Improvements in density functional theory methodology have made it a powerful tool in studying enzymatic reaction mechanisms, but concerns remain about the reliability of results for metalloenzymes. By using different DFT functionals and combining with experimental data, stable qualitative pictures of reaction mechanisms were obtained.
JOURNAL OF CHEMICAL PHYSICS
(2021)
Article
Plant Sciences
Alejandro Torrado, Macarena Iniesta-Pallares, Adrian Velazquez-Campoy, Consolacion Alvarez, Vicente Mariscal, Fernando P. Molina-Heredia
Summary: This study investigates the function and classification of cytochrome c(6)-like proteins in photosynthetic cyanobacteria, and provides new data on the phylogeny, genome localization, and functional properties of these proteins. The findings suggest that cytochrome c(6)-like proteins may interact with the cytochrome b(6)f complex in a similar way as cytochrome c(6), and cytochrome c(6)D can react with the specific copper-oxidase in heterocysts.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Yan Su, Hai Lin, Ying Tu, Meng-Meng Wang, Guan-Dong Zhang, Jin Yang, Hong-Ke Liu, Zhi Su
Summary: A new strategy utilizing mitochondrial dysfunction to overcome cisplatin resistance in lung cancer cells was proposed. Two mitochondria-targeted AIEgens, DP-PPh3 and TPE-PPh3, were synthesized and showed superior capacities to overcome cisplatin resistance by altering drug metabolism and blocking autophagic flux. The underlying molecular mechanism was fully revealed. These AIEgens not only promoted cytotoxicity towards cancer cells but also provided a new pathway for the treatment of metallodrug resistance.
Article
Chemistry, Multidisciplinary
Yuto Suzuki, Kazuaki Taguchi, Wataru Okamoto, Yuki Enoki, Teruyuki Komatsu, Kazuaki Matsumoto
Summary: In this study, methemoglobin-albumin clusters were developed as detoxifying agents for hydrogen sulfide (H2S) intoxication. These clusters have the ability to bind with H2S, thereby suppressing cell death and maintaining mitochondrial function. The results demonstrate that methemoglobin-albumin clusters possess suitable pharmacokinetic properties and biocompatibility for use as detoxifying agents against H2S intoxication.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Plant Sciences
Aprajita Kumari, Vemula Chandra Kaladhar, Nidhi Yadav, Pooja Singh, Kishorekumar Reddy, Kapuganti Jagadis Gupta
Summary: The location of NO production in mitochondrial cytochrome c oxidase and its role in mitochondrial biogenesis were investigated in Arabidopsis seedlings under osmotic stress and recovery. Osmotic stress reduced growth and mitochondrial number while increasing NO production. Nitrite application stimulated NO production and mitochondrial number in a NO-deficient mutant. COX6b(-)3 and COA6-L subunits were induced during osmotic stress and mutants impaired in these subunits showed reduced NO production and mitochondrial biogenesis. The COX6b-3 and COA6-L subunits interacted with a VQ27 motif-containing protein in the presence of NO, and a vq27 mutant displayed impaired mitochondrial biogenesis.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Cell Biology
Jay R. Laver, Diane Gbesemete, Adam P. Dale, Zoe C. Pounce, Carl N. Webb, Eleanor F. Roche, Jonathan M. Guy, Graham Berreen, Konstantinos Belogiannis, Alison R. Hill, Muktar M. Ibrahim, Muhammad Ahmed, David W. Cleary, Anish K. Pandey, Holly E. Humphries, Lauren Allen, Hans de Graaf, Martin C. Maiden, Saul N. Faust, Andrew R. Gorringe, Robert C. Read
Summary: Engineered N. lactamica can safely induce systemic, antigen-specific immune responses during carriage in humans, generating beneficial immune responses to heterologous antigens during sustained pharyngeal carriage.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Annette Fagerlund, Lene Idland, Even Heir, Trond Moretro, Marina Aspholm, Torii Lindback, Solveig Langsrud
Summary: Listeria monocytogenes is a deadly foodborne pathogen that is widespread in the environment. Whole-genome sequencing (WGS) is used to show the persistence of L. monocytogenes in natural, urban, and dairy farm environments. Clusters of almost identical isolates can be collected from geographically and temporally unrelated sources, highlighting the importance of understanding the genetic relationships between clinical isolates and L. monocytogenes isolates from various environments. The study emphasizes the need for reliable tools for source tracking, surveillance, and risk assessment in managing L. monocytogenes.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Claire E. Moulton-Brown, Tianer Feng, Shreiya Shivagni Kumar, Luxi Xu, Calvin Dytham, Thorunn Helgason, Julia M. Cooper, James W. B. Moir
Summary: Land management history has limited impact on the diversity and distribution of bacteria and denitrification genes in arable soils, which are primarily influenced by spatial location.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Sarah G. Earle, Mariya M. Lobanovska, Hayley Lavender, Changyan F. Tang, Rachel Exley, Elisa Ramos-Sevillano, Douglas F. Browning, Vasiliki Kostiou, Odile H. Harrison, Holly B. Bratcher, Gabriele Varani, Christoph M. Tang, Daniel J. Wilson, Martin C. J. Maiden
Summary: Genetic variations around the fHbp gene play a crucial role in determining susceptibility to invasive meningococcal disease (IMD), with certain variants affecting the expression of fHbp and promoting bacterial escape from complement-mediated killing. The study highlights the important connection between human and bacterial genetic variation in determining IMD susceptibility.
Article
Chemistry, Multidisciplinary
Alemayehu Godana Birhanu, Marta Gomez-Munoz, Shewit Kalayou, Tahira Riaz, Timo Lutter, Solomon Abebe Yimer, Markos Abebe, Tone Tonjum
Summary: This study uncovers the changes in the Mtb proteome in response to a sub-lethal dose of nitric oxide (NO) over several hours of exposure, revealing a two-phased proteomic response with specific proteins playing key roles throughout the different time points. The proteomic changes observed are compensatory in nature and contribute to various stress responses, providing new insights into how Mtb responds to NO at the proteomic level.
Article
Chemistry, Physical
Ebru Kocak Aslan, Vagolu Siva Krishna, Sanja J. Armakovic, Stevan Armakovic, Onur Sahin, Tone Tonjum, Miyase Gozde Gunduz
Summary: The emergence of drug-resistant strains of Mycobacterium tuberculosis (Mtb) has complicated efforts to eliminate tuberculosis. Developing new antitubercular drugs is crucial in addressing this challenge. Researchers used molecular hybridization to link the frontline drug isoniazid (INH) with various azole rings. These compounds showed excellent antimycobacterial activity and low cytotoxicity.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Microbiology
Chris Hadjineophytou, Jan Haug Anonsen, Tina Svingerud, Tatum D. Mortimer, Yonatan H. Grad, Nichollas E. Scott, Michael Koomey
Summary: Protein glycosylation systems in the genus Neisseria show species-specific variations, with different glycoprotein repertoires and target protein selection. The glycosylation of PilE, the pilin subunit of the type IV pilus colonization factor, is determined by the presence or absence of the PglO oligosaccharyltransferase in different species. This study highlights the importance of natural diversity in neisserial protein substrates and oligosaccharyltransferases in understanding the structure-function relationships of bacterial protein glycosylation systems.
Article
Chemistry, Medicinal
Ruveyde Keles Atici, Sengul Dilem Dogan, Miyase Gozde Gunduz, Vagolu Siva Krishna, Melina Chebaiki, Havard Homberset, Christian Lherbet, Lionel Mourey, Tone Tonjum
Summary: The emergence of drug-resistant strains of Mycobacterium tuberculosis (Mtb) has hindered efforts to combat tuberculosis. Researchers have synthesized 16 compounds using molecular hybridization and evaluated their antimycobacterial activity and cytotoxicity. Three derivatives showed promising activity against both drug-resistant and susceptible strains of Mtb.
DRUG DEVELOPMENT RESEARCH
(2022)
Article
Chemistry, Physical
Michael Tapera, Hueseyin Kekecmuhammed, Kader Sahin, Vagolu Siva Krishna, Christian Lherbet, Havard Homberset, Melina Chebaiki, Tone Tonjum, Lionel Mourey, Yunus Zorlu, Serdar Durdagi, Emin Saripinar
Summary: A series of novel thiourea derivatives were synthesized and tested for their antitubercular activity. Some compounds exhibited potent antitubercular activity with low cytotoxicity.
JOURNAL OF MOLECULAR STRUCTURE
(2022)
Article
Chemistry, Medicinal
Ebru Kocak Aslan, Muhammed Ihsan Han, Vagolu Siva Krishna, Rasoul Tamhaev, Cagatay Dengiz, Sengul Dilem Dogan, Christian Lherbet, Lionel Mourey, Tone Tonjum, Miyase Gozde Gunduz
Summary: This study presents a new strategy for modifying isoniazid (INH) to overcome resistance in Mycobacterium tuberculosis (Mtb) strains. Thirteen novel compounds were synthesized and screened for their antimycobacterial activity. The compounds showed low cytotoxicity and adherence to Lipinski's rule of five, making them potential candidates for antitubercular drugs.
Article
Immunology
Yih-Ling Tzeng, Soma Sannigrahi, Zachary Berman, Emily Bourne, Jennifer L. Edwards, Jose A. Bazan, Abigail Norris Turner, James W. B. Moir, David S. Stephens
Summary: A new clade of nonencapsulated Neisseria meningitidis has emerged and caused clusters of urethritis cases. It has acquired the denitrification pathway from Neisseria gonorrhoeae, which contributes to its adaptation and survival in a microaerobic urogenital environment.
INFECTION AND IMMUNITY
(2023)
Review
Genetics & Heredity
Kacper Kurzyp, Odile B. Harrison
Summary: This review examines the clinical manifestations of gonorrhoea, discusses their relation to disease severity and virulence factors, and explores the mechanisms and variations of these factors. The impact of phase variation during infection is studied, and whole-genome-sequence-based approaches for vaccine development are discussed. The potential of using whole-genome-sequence data to predict the severity of gonococcal infection is also explored.
MICROBIAL GENOMICS
(2023)
Article
Chemistry, Medicinal
Rukaiyya T. Girase, Iqrar Ahmad, Jong Min Oh, Hoon Kim, Bijo Mathew, Siva K. Vagolu, Tone Tonjum, Nisheeth C. Desai, Dharmarajan Sriram, Jyothi Kumari, Harun M. Patel
Summary: Clotrimazole has shown similar activity as Linezolid against drug-resistant tuberculosis and has less inhibition on MAO enzymes.
ACS MEDICINAL CHEMISTRY LETTERS
(2023)
Article
Microbiology
Ingun Lund Witso, Adelle Basson, Hilde Vinje, Ann-Katrin Llarena, Carlos Salas Bringas, Marina Aspholm, Yngvild Wasteson, Mette Myrmel
Summary: There is evidence that plastic particles can carry potentially pathogenic microorganisms. The composition of plastispheres on different types of plastic in a river was influenced by season and location. Detection of foodborne pathogenic bacteria and viruses in plastispheres raises concerns about the risk of contaminating food through irrigation water.
ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Immunology
Mingliang Chen, Odile B. Harrison, Holly B. Bratcher, Zhiyan Bo, Keith A. Jolley, Charlene M. C. Rodrigues, James E. Bray, Qinglan Guo, Xi Zhang, Min Chen, Martin C. J. Maiden
Summary: The study revealed the expansion of quinolone-resistant Neisseria meningitidis clone China(CC4821-R1-C/B) from China to other countries, highlighting the potential of causing a pandemic on a global scale.
EMERGING INFECTIOUS DISEASES
(2021)