Article
Multidisciplinary Sciences
Mario Castro, Grant Lythe, Jolanda Smit, Carmen Molina-Paris
Summary: Endosomes play a crucial role in mediating cell communication with the extracellular environment and regulating intracellular trafficking. Viruses can hijack endosomes to replicate and evade degradation, with endosomal maturation depending on Rab proteins. The relationship between endosomal active Rab levels and pH may contribute to variability in viral escape times and ultimately regulate the viability of a viral intracellular infection.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Viviane Kremling, Bernhard Loll, Szymon Pach, Ismail Dahmani, Christoph Weise, Gerhard Wolber, Salvatore Chiantia, Markus C. C. Wahl, Nikolaus Osterrieder, Walid Azab
Summary: The study reveals that EHV-1 and EHV-4 interact with MHC-I to initiate entry into equine cells. The crystal structures of gD1 and gD4 were determined, and protein-protein docking simulations, SPR analysis, and biological assays were performed to investigate the interaction. The identified key residues proved to be important for virus binding and altering them impaired virus growth in cells.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Lucia Maria Ghietto, Pedro Ignacio Gil, Paloma Olmos Quinteros, Emiliano Gomez, Franco Martin Piris, Patricia Kunda, Marta Contigiani, Maria Gabriela Paglini
Summary: This study investigates the early events of the replication cycle of Pixuna virus and Rio Negro virus, two mosquito-borne alphaviruses. The findings show that these viruses are primarily internalized through clathrin-mediated endocytosis and the low pH in endosomes is crucial for viral replication. Understanding the entry route of these viruses is important for understanding their pathogenesis and developing new antiviral strategies.
SCIENTIFIC REPORTS
(2022)
Article
Biochemical Research Methods
Jim Warwicker
Summary: This study explored the stability of spike proteins from SARS-CoV-2 and other coronaviruses under different conditions, identifying key residues that may affect their pH-dependence. These findings provide new insights into the infection mechanism and mutations of SARS-CoV-2.
BRIEFINGS IN BIOINFORMATICS
(2021)
Article
Microbiology
Krishanthi S. Karunatilaka, David J. Filman, Mike Strauss, Joseph J. Loparo, James M. Hogle
Summary: The study developed a fluorescence imaging assay to visualize poliovirus genome release using a synthetic vesicle system. The results not only provide new mechanistic insights into poliovirus genome translocation, but also offer a cell-free assay to bridge gaps in understanding of this process in other nonenveloped viruses.
Article
Microbiology
You Zhang, Joanne N. York, Melinda A. Brindley, Jack H. N. Nunberg, Gregory B. Melikyan
Summary: The fusion activity of the Lassa virus glycoprotein complex is accompanied by an increase in viral membrane permeability and acidification of the virus core, and can be inhibited by disrupting the fusion structure.
Article
Engineering, Biomedical
Kwan-Jip Kim, Gijeong Kim, Jin-Ho Bae, Ji-Joon Song, Hak-Sung Kim
Summary: This study develops a virus-like particle (VLP) that shows pH-responsive assembly and disassembly for targeted drug delivery through the computational design of bacteriophage P22 coat proteins. The cryo-EM structure of the designed VLP is determined, demonstrating the underlying mechanism of pH responsiveness. The utility of the VLP is exemplified by delivering a tumor-targeting cytotoxic protein in a pH-dependent manner.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Stanislav Kler, Ran Zalk, Alexander Upcher, Idit Kopatz
Summary: This article reports a new type of nanoparticle with a capsid composed of three SV40 virus capsid proteins, which closely mimics the intracellular pathway of the virus and has potential applications in gene delivery.
Review
Chemistry, Multidisciplinary
Zhi-Gang Wang, Shu-Lin Liu, Dai-Wen Pang
Summary: The QD-based single-virus tracking (QSVT) technique has immense potential in virology, revealing infection mechanisms of viruses through tracking individual virus behaviors. QSVT has advantages of high brightness, extraordinary photostability, color-tunable emission, and abundant surface ligands, providing detailed dynamic information in the viral lifecycle.
ACCOUNTS OF CHEMICAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Valentina Gentili, Giulia Turrin, Paolo Marchetti, Sabrina Rizzo, Giovanna Schiuma, Silvia Beltrami, Virginia Cristofori, Davide Illuminati, Greta Compagnin, Claudio Trapella, Roberta Rizzo, Daria Bortolotti, Anna Fantinati
Summary: Compounds synthesized by researchers showed significant antiviral activity in human cells, with 9e demonstrating a lasting inhibitory effect on viral replication for over 7 days, indicating support for the use of these amphipathic fusion inhibitors in treating HHV-6 infections.
BIOORGANIC CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Lien De Caluwe, Kevin K. Arien, Koen Bartholomeeusen
Summary: Chikungunya virus (CHIKV) is a re-emerging arthropod-borne virus that has spread globally, requiring entry into host cells for replication. This review summarizes the steps and mechanisms of CHIKV entry into human and mosquito host cells, discussing factors related to tissue tropism and potential therapeutic targeting.
TRENDS IN MICROBIOLOGY
(2021)
Article
Virology
Katrina A. Gianopulos, Tri Komala Sari, Darin J. Weed, Suzanne M. Pritchard, Anthony Nicola
Summary: Low endosomal pH facilitates herpesvirus entry in a cell-specific manner. The gC protein of HSV-1 plays a critical role in low-pH entry pathway by regulating the conformational changes of viral envelope glycoprotein B (gB). This study reveals that low pH triggers conformational changes in gC itself, and a specific gC epitope is important for low-pH entry inhibition.
JOURNAL OF VIROLOGY
(2022)
Article
Immunology
Xiao Ma, Zhenghong Yuan, Zhigang Yi
Summary: This study revealed the key residues of the E protein involved in the entry and egress of Zika virus. The results showed that DIII-mediated E trimerization is essential for viral egress, while key residues in DII participate in virus entry.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Virology
Young-Sheng Chang, Li-Wei Chu, Zan-Yu Chen, Joh-Sin Wu, Wen-Chi Su, Chia-Jui Yang, Yueh-Hsin Ping, Cheng-Wen Lin
Summary: SARS-CoV-2 structural proteins can assemble into virus-like particles (VLPs) in cells, known as SCoV2-SEM VLPs, which have similar structure and function to the authentic virus. Fluorescence microscopy and inhibitor treatments confirmed the infectious mechanism of SCoV2-SEM VLPs, resembling that of the real virus.
Article
Virology
Miyuki Kimura, Kazuki Sekiguchi, Shoko Okitsu, Hiroshi Ushijima, Hideki Tani
Summary: This study established a highly quantitative detection system for HuNoV VLP entry into live cells and found Caco-2 cells to be the most susceptible. The effects of treatment conditions on VLP entry into live cells were also investigated.
Editorial Material
Biochemistry & Molecular Biology
Rolf Boelens, Dmitri Svergun, Jan Ellenberg, Stephen Cusack, Martin A. Walsh, Lucia Banci, Harald Schwalbe, Anastassis Perrakis, Vladimir Sklenar, Jose Maria Carazo Garcia, Hartmut Oschkinat, Andrew Thompson, Marjolein Thunnissen, Bram Koster, Gordon Leonard, Bernhard Brutscher, David I. Stuart, Poul Nissen, Hanna Wacklin, Andras Perczel, Margarida Archer, Rik Wierenga, Georgios A. Spyroulias, Joel Sussman
Article
Chemistry, Medicinal
Yuguang Zhao, Jingshan Ren, Elizabeth E. Fry, Julia Xiao, Alain R. Townsend, David I. Stuart
JOURNAL OF MEDICINAL CHEMISTRY
(2018)
Article
Chemistry, Medicinal
Jingshan Ren, Yuguang Zhao, Elizabeth E. Fry, David I. Stuart
JOURNAL OF MEDICINAL CHEMISTRY
(2018)
Article
Biochemical Research Methods
Jonathan M. Grimes, David R. Hall, Alun W. Ashton, Gwyndaf Evans, Robin L. Owen, Armin Wagner, Katherine E. McAuley, Frank von Delft, Allen M. Orville, Thomas Sorensen, Martin A. Walsh, Helen M. Ginn, David I. Stuart
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2018)
Article
Virology
Olga Gonzalez-Lopez, Efrain E. Rivera-Serrano, Fengyu Hu, Lucinda Hensley, Kevin L. McKnight, Jingshan Ren, David Stuart, Elizabeth E. Fry, Stanley M. Lemon
JOURNAL OF VIROLOGY
(2018)
Article
Multidisciplinary Sciences
Gareth Shimmon, Abhay Kotecha, Jingshan Rene, Amin S. Astor, Joseph Newman, Stephen Berryman, Eleanor M. Cottam, Sarah Gold, Toby J. Tuthil, Donald P. King, Emiliana Brocchi, Andrew M. Q. King, Ray Owens, Elizabeth E. Fry, David Stuart, Alison Burman, Terry Jackson
Article
Multidisciplinary Sciences
Helen M. E. Duyvesteyn, Abhay Kotecha, Helen M. Ginn, Corey W. Hecksel, Emma V. Beale, Felix de Haas, Gwyndaf Evans, Peijun Zhang, Wah Chiu, David I. Stuart
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Multidisciplinary Sciences
Lucy C. Walters, Karl Harlos, Simon Brackenridge, Daniel Rozbesky, Jordan R. Barrett, Vitul Jain, Thomas S. Walter, Chris A. O'Callaghan, Persephone Borrow, Mireille Toebes, Scott G. Hansen, Jonah Sacha, Shaheed Abdulhaqq, Justin M. Greene, Klaus Fruh, Emily Marshall, Louis J. Picker, E. Yvonne Jones, Andrew J. McMichael, Geraldine M. Gillespie
NATURE COMMUNICATIONS
(2018)
Article
Multidisciplinary Sciences
Abhay Kotecha, Eva Perez-Martin, Yongjie Harvey, Fuquan Zhang, Serban L. Ilca, Elizabeth E. Fry, Ben Jackson, Francois Maree, Katherine Scott, Corey W. Hecksel, Michiel M. Harmsen, Valerie Mioulet, Britta Wood, Nick Juleff, David I. Stuart, Bryan Charleston, Julian Seago
SCIENTIFIC REPORTS
(2018)
Correction
Multidisciplinary Sciences
Lucy C. Walters, Karl Harlos, Simon Brackenridge, Daniel Rozbesky, Jordan R. Barrett, Vitul Jain, Thomas S. Walter, Chris A. O'Callaghan, Persephone Borrow, Mireille Toebes, Scott G. Hansen, Jonah B. Sacha, Shaheed Abdulhaqq, Justin M. Greene, Klaus Fruh, Emily Marshall, Louis J. Picker, E. Yvonne Jones, Andrew J. McMichael, Geraldine M. Gillespie
NATURE COMMUNICATIONS
(2018)
Article
Multidisciplinary Sciences
Kamel El Omari, Sai Li, Abhay Kotecha, Thomas S. Walter, Eduardo A. Bignon, Karl Harlos, Pentti Somerharju, Felix De Haas, Daniel K. Clare, Mika Molin, Felipe Hurtado, Mengqiu Li, Jonathan M. Grimes, Dennis H. Bamford, Nicole D. Tischler, Juha T. Huiskonen, David I. Stuart, Elina Roine
NATURE COMMUNICATIONS
(2019)
Article
Multidisciplinary Sciences
Luigi De Colibus, Elina Roine, Thomas S. Walter, Serban L. Ilca, Xiangxi Wang, Nan Wang, Alan M. Roseman, Dennis Bamford, Juha T. Huiskonen, David Stuart
NATURE COMMUNICATIONS
(2019)
Article
Multidisciplinary Sciences
Victoria A. Avanzato, Kasopefoluwa Y. Oguntuyo, Marina Escalera-Zamudio, Bernardo Gutierrez, Michael Golden, Sergei L. Kosakovsky Pond, Rhys Pryce, Thomas S. Walter, Jeffrey Seow, Katie J. Doores, Oliver G. Pybus, Vincent J. Munster, Benhur Lee, Thomas A. Bowden
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Biochemistry & Molecular Biology
Wanwisa Dejnirattisai, Daming Zhou, Helen M. Ginn, Helen M. E. Duyvesteyn, Piyada Supasa, James Brett Case, Yuguang Zhao, Thomas S. Walter, Alexander J. Mentzer, Chang Liu, Beibei Wang, Guido C. Paesen, Jose Slon-Campos, Cesar Lopez-Camacho, Natasha M. Kafai, Adam L. Bailey, Rita E. Chen, Baoling Ying, Craig Thompson, Jai Bolton, Alex Fyfe, Sunetra Gupta, Tiong Kit Tan, Javier Gilbert-Jaramillo, William James, Michael Knight, Miles W. Carroll, Donal Skelly, Christina Dold, Yanchun Peng, Robert Levin, Tao Dong, Andrew J. Pollard, Julian C. Knight, Paul Klenerman, Nigel Temperton, David R. Hall, Mark A. Williams, Neil G. Paterson, Felicity K. R. Bertram, C. Alistair Siebert, Daniel K. Clare, Andrew Howe, Julika Radecke, Yun Song, Alain R. Townsend, Kuan-Ying A. Huang, Elizabeth E. Fry, Juthathip Mongkolsapaya, Michael S. Diamond, Jingshan Ren, David Stuart, Gavin R. Screaton
Summary: Antibodies play a crucial role in immune protection against SARS-CoV-2, with some being used as therapeutics. A study identified 377 human monoclonal antibodies, focusing on 80 that bind the virus spike, and found that most highly inhibitory antibodies can block the virus-receptor interaction. Novel binding modes of potent inhibitory antibodies were discovered, showing potential for prophylactic or therapeutic use in animal models.
Article
Biology
Helen M. E. Duyvesteyn, Jingshan Ren, Thomas S. Walter, Elizabeth E. Fry, David I. Stuart
COMMUNICATIONS BIOLOGY
(2020)