Article
Microbiology
Myungseo Park, Jinshil Kim, Jill Feinstein, Kevin S. Lang, Sangryeol Ryu, Byeonghwa Jeon
Summary: Antibiotic tolerance in C. jejuni leads to the development of antibiotic resistance, particularly fluoroquinolone resistance. Exposure to ciprofloxacin or tetracycline induces antibiotic tolerance and promotes the emergence of fluoroquinolone-resistant C. jejuni clones. Alkyl hydroperoxide reductase plays a critical role in reducing the development of fluoroquinolone resistance after tolerance induction.
MICROBIOLOGY SPECTRUM
(2022)
Article
Public, Environmental & Occupational Health
Jose A. Rodrigues, Wonhee Cha, Rebekah E. Mosci, Sanjana Mukherjee, Duane W. Newton, Paul Lephart, Hossein Salimnia, Walid Khalife, James T. Rudrik, Shannon D. Manning
Summary: In a four-year active surveillance study at four metropolitan hospitals in Michigan, an increasing trend in Campylobacter jejuni infections was observed. Analysis of 214 C. jejuni isolates revealed widespread antibiotic resistance, with differences in infection rates by hospital type and patient characteristics associated with disease severity.
FRONTIERS IN PUBLIC HEALTH
(2021)
Article
Infectious Diseases
Debora Brito Goulart, Ashenafi Feyisa Beyi, Zuowei Wu, Mehmet Cemal Adiguzel, Anastasia Schroeder, Kritika Singh, Changyun Xu, Melda Meral Ocal, Renee Dewell, Grant A. Dewell, Paul J. Plummer, Qijing Zhang, Orhan Sahin
Summary: This study evaluated the impact of danofloxacin treatment on the development of fluoroquinolone-resistant Campylobacter in cattle. The results showed that the treatment led to an increase in fluoroquinolone-resistant strains, mainly due to enrichment of preexisting resistant strains.
Article
Infectious Diseases
Debora Brito Goulart, Ashenafi Feyisa Beyi, Zuowei Wu, Mehmet Cemal Adiguzel, Samantha Wilson, Changyun Xu, Jinji Pang, Renee Dewell, Grant A. Dewell, Paul J. Plummer, Qijing Zhang, Orhan Sahin
Summary: Enrofloxacin treatment has a minimal impact on the development of fluoroquinolone resistance in Campylobacter jejuni in cattle.
Article
Veterinary Sciences
Niamh Caffrey, Agnes Agunos, Sheryl Gow, Karen Liljebjelke, Cheryl L. Waldner, Chunu Mainali, Sylvia L. Checkley
Summary: This study utilized data from the Canadian Integrated Program for Antimicrobial Resistance Surveillance to investigate factors associated with the isolation of FQr C. jejuni in broiler flocks. The findings suggest that bird type, use of virginiamycin, rodent control measures, flock size, and water line disinfection methods are key factors influencing the prevalence of FQr C. jejuni. Biosecurity and sanitation procedures play a significant role in the dissemination of FQr C. jejuni in broiler flocks.
PREVENTIVE VETERINARY MEDICINE
(2021)
Article
Veterinary Sciences
Debora Brito Goulart, Qijing Zhang, Orhan Sahin
Summary: Human enterocolitis is commonly caused by the Gram-negative bacterium Campylobacter jejuni. The emergence of fluoroquinolone-resistant (FQ-R) Campylobacter has increased in poultry and cattle, which are important reservoirs of the bacteria. In this study, it was found that FQ-R Campylobacter strains from cattle had slightly higher fitness advantage over susceptible strains, but the emergence of FQ-R mutants was primarily influenced by bacterial cell density and antibiotic concentration. These findings provide explanations for the prevalence of FQ-R Campylobacter in cattle production and the limited development of FQ-R Campylobacter in response to treatment in cattle intestines.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Public, Environmental & Occupational Health
Rima D. Shrestha, Agnes Agunos, Sheryl P. Gow, Csaba Varga
Summary: This study investigated antimicrobial resistance in turkeys in Canada and found a high prevalence of resistant Campylobacter isolates. Despite minimal use of tetracyclines and fluoroquinolones, a significant proportion of C. jejuni and C. coli isolates exhibited resistance to these drugs. Regional differences in resistance prevalence were observed, and certain antimicrobial classes may have contributed to the maintenance of resistance.
EPIDEMIOLOGY AND INFECTION
(2023)
Review
Immunology
Se-Hun Kim, Ramachandran Chelliah, Sudha Rani Ramakrishnan, Ayyappasamy Sudalaiyadum Perumal, Woo-Suk Bang, Momna Rubab, Eric Banan-Mwine Daliri, Kaliyan Barathikannan, Fazle Elahi, Eunji Park, Hyeon Yeong Jo, Su-Bin Hwang, Deog Hwan Oh
Summary: Campylobacter spp. are the leading cause of bacterial colon infections in humans globally, and their stress-adaptation mechanisms differ from other enteropathogenic bacteria, allowing them to survive in diverse environments and enhance their resistance mechanisms.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Ying-Shu Liao, Bo-Han Chen, Ru-Hsiou Teng, You-Wun Wang, Jui-Hsien Chang, Shiu-Yun Liang, Chi-Sen Tsao, Yu-Ping Hong, Hui-Yung Sung, Chien-Shun Chiou
Summary: Research in Taiwan has shown that C. coli and C. jejuni are highly resistant to most therapeutic antimicrobials. Whole-genome sequencing was used to analyze genetic diversity among isolates and genetic resistance determinants associated with antimicrobial resistance.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Microbiology
Mehmet Cemal Adiguzel, Debora Brito Goulart, Zuowei Wu, Jinji Pang, Seyda Cengiz, Qijing Zhang, Orhan Sahin
Summary: The study found that FQ-resistant Campylobacter strains mostly carry mutations in the gyrA gene and contain various spacer sequences within the CRISPR system, with many showing high nucleotide homology to known Campylobacter phages. While most spacer sequences matched known phages, there were also some unidentified spacer sequences present.
Article
Food Science & Technology
Malgorzata Emanowicz, Joseph Meade, Catherine Burgess, Declan Bolton, John Egan, Helen Lynch, Lisa O'Connor, Aidan Coffey, Brigid Lucey, Montserrat Gutierrez, William Byrne, Rosemarie Slowey, Paul Whyte
Summary: Phenotypic antimicrobial resistance testing was conducted on 160 Campylobacter jejuni isolates from broiler samples to determine prevalence and distribution of resistance profiles. Whole genome sequencing of 132 isolates revealed a high correlation between resistant phenotypes and genotypes, with identified resistance determinants including tet(O) genes and gyrA p. T86I mutation. Multiple sequence types (ST) were identified, with ST-257 being prevalent, and virulence genes associated with human infection and broiler colonization were detected.
Article
Food Science & Technology
Shanwei Tong, Luyao Ma, Jennifer Ronholm, William Hsiao, Xiaonan Lu
Summary: Campylobacter is a leading cause of bacterial gastroenteritis worldwide, and whole genome sequencing has been proven to be a robust and efficient tool for monitoring this microbe in food chain surveillance.
CURRENT OPINION IN FOOD SCIENCE
(2021)
Article
Multidisciplinary Sciences
Xiaofang Wu, Lei Ji, Yuehua Shen, Liping Chen, Deshun Xu, Fenfen Dong
Summary: Thirteen pathogenic Campylobacter strains were isolated from diarrhea cases and raw poultry meat products in Huzhou, including 11 Campylobacter jejuni and 2 Campylobacter coli strains. These strains contain multiple virulence-related genes and show high resistance to nalidixic acid, ciprofloxacin, and tetracycline. Genomic sequencing revealed the presence of tet(O) and blaOXA resistance genes in all C. jejuni strains and multiple drug resistance genes in 2 C. coli strains.
Article
Agriculture, Dairy & Animal Science
Zolzaya Byambajav, Erdenebat Bulgan, Yuji Hirai, Momoko Nakayama, Misaki Tanaka, Yurika Nitta, Akio Suzuki, Takashi Umemura, Bold Altankhuu, Alimaa Tsagaan, Batbaatar Vanaabaatar, Erdenebaatar Janchivdorj, Nyam-Osor Purevdorj, Narantuya Ayushjav, Takeshi Yamasaki, Motohiro Horiuchi
Summary: This study isolated and characterized Campylobacter spp. from chickens in Mongolia, finding resistance to multiple antibiotics. Some C. jejuni/C. coli isolates were resistant to nalidixic acid and fluoroquinolones, with specific genetic mechanisms for resistance identified. Multilocus sequence typing and phylogenetic analyses revealed genetic variation and potential links to other countries. This study represents the first report on antimicrobial resistance of Campylobacter spp. in farm animals in Mongolia, providing valuable insights for antibiotic use in this region.
Review
Biochemistry & Molecular Biology
Amber D. Gabbert, Jennifer L. Mydosh, Prabhat K. Talukdar, Lisa M. Gloss, Jason E. McDermott, Kerry K. Cooper, Geremy C. Clair, Michael E. Konkel
Summary: Campylobacter jejuni is the most common bacterial cause of gastroenteritis worldwide, causing millions of cases of inflammatory diarrhea. It is associated with post-infection complications in developed countries and malnutrition and growth-stunting in low- and middle-income countries. Our understanding of C. jejuni pathogenesis is incomplete, but we focus on the secretion systems and their role in host-cell interactions and disease establishment. The flagellar type III secretion system and putative effectors are of particular interest.
Article
Microbiology
Qingyun Li, Xiyu Peng, Eric R. Burrough, Orhan Sahin, Stacie A. Gould, Nicholas K. Gabler, Crystal L. Loving, Karin S. Dorman, John F. Patience
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Veterinary Sciences
Panchan Sitthicharoenchai, Rachel Derscheid, Kent Schwartz, Nubia Macedo, Orhan Sahin, Xuhua Chen, Ganwu Li, Rodger Main, Eric Burrough
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2020)
Article
Infectious Diseases
Mehmet Cemal Adiguzel, Alper Baran, Zuowei Wu, Seyda Cengiz, Lei Dai, Cihan Oz, Esma Ozmenli, Debora Brito Goulart, Orhan Sahin
Summary: This study detected an E. coli isolate carrying the mcr-1 gene from retail raw chicken meat in Turkey. Whole genome sequencing revealed that this isolate was more closely related to mcr-1 carrying E. coli strains reported from different parts of the world than to those from Turkey. These findings underline the global emergence and spread of mcr-mediated colistin resistance in bacteria with zoonotic potential within animals and the food chain.
MICROBIAL DRUG RESISTANCE
(2021)
Article
Immunology
Brandon Ruddell, Alan Hassall, Orhan Sahin, Qijing Zhang, Paul J. Plummer, Amanda J. Kreuder
Summary: The genes metA and metB are essential for l-methionine production in Campylobacter jejuni in the absence of luxS, and play a critical role in normal chicken colonization by the bacteria.
INFECTION AND IMMUNITY
(2021)
Article
Microbiology
Gregory H. Tyson, Olgica Ceric, Jake Guag, Sarah Nemser, Stacey Borenstein, Durda Slavic, Sarah Lippert, Rebecca McDowell, Aparna Krishnamurthy, Shannon Korosec, Cheryl Friday, Neil Pople, Matthew E. Saab, Julie-Helene Fairbrother, Isabelle Janelle, Deanna McMillan, Yugendar R. Bommineni, David Simon, Shipra Mohan, Susan Sanchez, Ashley Phillips, Paula Bartlett, Hemant Naikare, Cynthia Watson, Orhan Sahin, Chloe Stinman, Leyi Wang, Carol Maddox, Vanessa DeShambo, Kenitra Hendrix, Debra Lubelski, Amy Burklund, Brian Lubbers, Debbie Reed, Tracie Jenkins, Erdal Erol, Mukeshbhai Patel, Stephan Locke, Jordan Fortner, Laura Peak, Udeni Balasuriya, Rinosh Mani, Niesa Kettler, Karen Olsen, Shuping Zhang, Zhenyu Shen, Martha Pulido Landinez, Jay Kay Thornton, Anil Thachil, Melissa Byrd, Megan Jacob, Darlene Krogh, Brett Webb, Lynn Schaan, Amar Patil, Sarmila Dasgupta, Shannon Mann, Laura B. Goodman, Rebecca June Franklin-Guild, Renee R. Anderson, Patrick K. Mitchell, Missy Aprea, Brittany D. Cronk, Jing Cui, Dominika Jurkovic, Melanie Prarat, Yan Zhang, Katherine Shiplett, Dubra Diaz Campos, Joany Van Balen Rubio, Akhilesh Ramanchandran, Scott Talent, Deepanker Tewari, Nagaraja Thirumalapura, Donna Kelly, Denise Barnhart, Lacey Hall, Shelley Rankin, Jaclyn Dietrich, Stephen Cole, Joy Scaria, Linto Antony, Sara D. Lawhon, Jing Wu, Christine McCoy, Kelly Dietz, Rebecca Wolking, Trevor Alexander, Claire Burbick, Renate Reimschuessel
Summary: This study evaluated the correlation between resistance genotypes and phenotypes for Staphylococcus pseudintermedius by conducting antimicrobial susceptibility testing and WGS on isolates collected from dogs in the United States. The results showed a strong correlation between resistance genotypes and clinical resistance phenotypes, with findings comparable to studies on human pathogens such as Staphylococcus aureus and Salmonella enterica. Notable discoveries included the presence of mecA gene in 32.3% of isolates, which correlated with oxacillin resistance 97.0% of the time, and the identification of a novel rpoB mutation likely encoding rifampin resistance. The study highlights the value of using WGS to assess antimicrobial resistance in veterinary pathogens and uncover potential new mechanisms of resistance.
VETERINARY MICROBIOLOGY
(2021)
Article
Public, Environmental & Occupational Health
Atsushi Hinenoya, Xing-Ping Li, Ximin Zeng, Orhan Sahin, Rodney A. Moxley, Catherine M. Logue, Barbara Gillespie, Shinji Yamasaki, Jun Lin
Summary: This study investigated the prevalence and characteristics of E. albertii in US domestic and food animals, revealing chickens as an important reservoir for E. albertii. The isolated chicken E. albertii strains displayed multidrug resistance and phylogenetic closeness to human-derived strains. Further large-scale surveys in poultry production are warranted in the future.
ZOONOSES AND PUBLIC HEALTH
(2021)
Article
Microbiology
Mehmet Cemal Adiguzel, Debora Brito Goulart, Zuowei Wu, Jinji Pang, Seyda Cengiz, Qijing Zhang, Orhan Sahin
Summary: The study found that FQ-resistant Campylobacter strains mostly carry mutations in the gyrA gene and contain various spacer sequences within the CRISPR system, with many showing high nucleotide homology to known Campylobacter phages. While most spacer sequences matched known phages, there were also some unidentified spacer sequences present.
Article
Veterinary Sciences
Michael J. Yaeger, Orhan Sahin, Paul J. Plummer, Zuowei Wu, Judith A. Stasko, Qijing Zhang
Summary: The main lesions observed in cases of Campylobacter abortion in sheep included placentitis with placental bacterial colonies, placental vasculitis, and fetal pneumonia. Experimentally induced abortions provided better-preserved specimens for defining placental changes more precisely.
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2021)
Article
Multidisciplinary Sciences
Michael Yaeger, Jonathan P. Mochel, Zuowei Wu, Paul Plummer, Orhan Sahin, Joseph Smith, Melda Ocal, Ashenafi Beyi, Changyun Xu, Qijing Zhang, Ronald W. Griffith
Summary: Prior IV administration of C. jejuni appeared to substantially alter the pharmacokinetics of tulathromycin in pregnant ewes, reducing both the peak plasma concentrations and elimination half-life compared to previous reports. Further controlled trials are needed to confirm these observations.
Article
Biotechnology & Applied Microbiology
Fuat Aydin, Secil Abay, Tuba Kayman, Emre Karakaya, Hamit Kaan Mustak, Inci Basak Mustak, Nuket Bilgen, Muammer Goncuoglu, Ayhan Duzler, Ozgur Guran, Orhan Sahin, Izzet Burcin Saticioglu
Summary: A polyphasic taxonomic study was conducted on 74 Gram-negative microorganisms isolated from fecal samples of Anatolian ground squirrels in Kayseri, Turkey, which may represent a novel bacterial species named Campylobacter anatolicus.
SYSTEMATIC AND APPLIED MICROBIOLOGY
(2021)
Article
Microbiology
Ashenafi Feyisa Beyi, Debora Brito-Goulart, Tyler Hawbecker, Brandon Ruddell, Alan Hassall, Renee Dewell, Grant Dewell, Orhan Sahin, Qijing Zhang, Paul J. Plummer
Summary: The study found that high and low doses of enrofloxacin can impact the gut microbiota and resistome of calves, with high doses causing a larger shift in bacterial genera. Using metagenomic ProxiMeta Hi-C, multiple unique antimicrobial resistance genes were detected, with high doses favoring clonal expansion of ARG-carrying bacterial hosts. Hi-C sequencing provides valuable insights into ARG ecology in complex samples, but further validation is needed due to limitations in sample size and sequencing depth.
Article
Microbiology
Ashenafi Feyisa Beyi, Debora Brito-Goulart, Tyler Hawbecker, Clare Slagel, Brandon Ruddell, Alan Hassall, Renee Dewell, Grant Dewell, Orhan Sahin, Qijing Zhang, Paul J. Plummer
Summary: The use of danofloxacin can lead to changes in the gut microbiota of calves, resulting in shifts in the composition of several bacterial taxa and affecting the relationship between Campylobacter and other genera. Danofloxacin may also contribute to the selection and enrichment of resistance genes, even against antibiotics unrelated to danofloxacin.
Article
Multidisciplinary Sciences
Ashenafi Feyisa Beyi, Jonathan P. Mochel, Geraldine Magnin, Tyler Hawbecker, Clare Slagel, Grant Dewell, Renee Dewell, Orhan Sahin, Johann F. Coetzee, Qijing Zhang, Paul J. Plummer
Summary: The pharmacokinetics of danofloxacin and enrofloxacin were evaluated in plasma and feces of healthy and infected calves. The study showed that the fecal concentration of danofloxacin was lower in the infected group and FQs had higher overall concentrations in feces compared to plasma.
SCIENTIFIC REPORTS
(2022)
Article
Immunology
Panchan Sitthicharoenchai, Eric R. Burrough, Bailey L. Arruda, Orhan Sahin, Jessica G. dos Santos, Drew R. Magstadt, Pablo E. Pineyro, Kent J. Schwartz, Michael C. Rahe
Summary: Streptococcus gallolyticus was identified as a causative agent in 7.59% of valvular endocarditis cases in swine. This emerging infection in swine may provide insights for the study of endocarditis in humans.
EMERGING INFECTIOUS DISEASES
(2022)
Article
Microbiology
Zuowei Wu, Orhan Sahin, Qijing Zhang
Summary: Campylobacter hepaticus has been identified as the causative agent of chicken spotty liver disease. The complete genome sequence of C. hepaticus strain USA52 from the United States has been reported, consisting of a chromosome of 1,509,100 bp with an average GC content of 28.02%.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)