Article
Oncology
Omid Fotouhi, Sheikh Nizamuddin, Stephanie Falk, Oliver Schilling, Ruth Knuechel-Clarke, Martin L. Biniossek, H. T. Marc Timmers
Summary: Alternative splicing of UTX transcripts plays a crucial role in its function, with the absence of exon 14 resulting in the inability of UTX transcripts to localize to the nucleus and lesser interactions with other chromatin regulatory complexes.
Article
Biochemistry & Molecular Biology
Joshua Meehan, Suzanne M. McDermott, Alasdair Ivens, Zachary Goodall, Zihao Chen, Zihao Yu, Jia Woo, Tyler Rodshagen, Laura McCleskey, Rebecca Sechrist, Kenneth Stuart, Lanying Zeng, Silvi Rouskin, Nicholas J. Savill, Achim Schnaufer, Xiuren Zhang, Jorge Cruz-Reyes
Summary: U-insertion/deletion RNA editing in trypanosome mitochondria is guided by gRNAs and may control respiration developmentally. Accurate canonical editing is required for normal cell growth, even though most edits do not match the canonical pattern. KREH2 helicase differentially controls extensive non-canonical editing via a novel regulatory gRNA, leading to the formation of stable RNA structures.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Pathology
Paul W. Harms, Monique E. Verhaegen, Kevin Hu, Steven M. Hrycaj, May P. Chan, Chia-Jen Liu, Marina Grachtchouk, Rajiv M. Patel, Aaron M. Udager, Andrzej A. Dlugosz
Summary: MCC and SCCIS show significant overlap in gene mutations, with no unique gene mutations found in all cases of MCC. Transcriptome analysis reveals large differences in gene expression between MCC and SCCIS, including epidermal markers and immune genes. Immunohistochemistry studies show increased expression of SOX2 in the MCC component.
Article
Biochemistry & Molecular Biology
Chang-Hyun Kim, Sang-Moo Park, Sun-jae Lee, Young-Dae Kim, Se-Hwan Jang, Seon-Min Woo, Taeg-Kyu Kwon, Zee-Yong Park, Ik-Joo Chung, Hye-Ran Kim, Chang-Duk Jun
Summary: NSrp70 is selectively expressed in developing thymocytes, highest at the DP stage. It regulates the cell cycle and survival of thymocytes by controlling the alternative processing of various RNA splicing factors. Knockout of NSrp70 in mice resulted in severe defects in T cell maturation and peripheral lymphopenia.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Multidisciplinary Sciences
Norimasa Iwanami, Andreas S. Richter, Katarzyna Sikora, Thomas Boehm
Summary: Unconventional T cells, such as iNKT cells, play an important role in vertebrate immune defenses. The study suggests that Tnpo3 is involved in regulating the splicing of pre-mRNA encoding the characteristic TCR alpha chain of iNKT cells.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Meagan McMahon, George O'Dell, Jessica Tan, Andras Sarkozy, Mate Vadovics, Juan Manuel Carreno, Eduard Puente-Massaguer, Hiromi Muramatsu, Csaba Bajusz, Willemijn Rijnink, Mitchell Beattie, Ying K. Tam, Ericka Kirkpatrick Roubidoux, Isabel Francisco, Shirin Strohmeier, Masaru Kanekiyo, Barney S. Graham, Florian Krammer, Norbert Pardi
Summary: This study demonstrates the use of nucleoside-modified mRNA-LNP vaccines to target multiple influenza virus antigens. The results show that the vaccine induces antigen-specific immune responses and provides effective protection against various influenza viruses in mice.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Tiantian Gan, Ziwei Lin, Lijun Bao, Tian Hui, Xiaopeng Cui, Yanzhen Huang, Hexin Wang, Chao Su, Feng Jiao, Minjuan Zhang, Yonghua Qian
Summary: Through analyzing the physiological changes and proteomic profiles of salt-tolerant and salt-sensitive mulberry varieties under salt stress, it was found that the salt-tolerant variety showed stronger adaptation at the proteomic level, with phenylpropanoid biosynthesis playing an important role in mulberry salt tolerance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Abu Osman, Bingyu Yan, Ying Li, Kevin D. Pavelko, Jasmine Quandt, Abdulrahman Saadalla, Mahendra Pal Singh, Majid Kazemian, Fotini Gounari, Khashayarsha Khazaie
Summary: Research shows that TCF-1 collaborates with FOXP3 to regulate suppressive activities of T-reg cells, with TCF-1 deficiency promoting inflammation and compromising T-cell cytotoxicity control. TCF-1 primarily suppresses transcription of co-bound genes by Foxp3, and deficiency in TCF-1 leads to activation and characteristics of the T(H)17 subset in T-reg cells. TCF-1 deficiency in T-reg cells can determine colorectal cancer outcome by regulating inflammation and T-cell cytotoxicity.
Article
Biochemical Research Methods
Desmond O. Agwunobi, Mengxue Li, Ningmei Wang, Guomin Chang, Xiaojing Zhang, Xiaomin Xue, Zhijun Yu, Hui Wang, Jingze Liu
Summary: This study investigated the molecular mechanism of lemongrass essential oil on the Haemaphysalis longicornis tick using proteomic analysis. Pathway analysis revealed the activation of metabolic pathways mediated by oxidoreductases and transferases. Furthermore, evidence of cytotoxic mode of action was found, involving reactive oxygen species, mitochondrial calcium overload, mitochondrial uncoupling, depolarization, and ATP depletion. The RNAi of a major detoxification enzyme also caused morphological alterations, which requires further investigation.
Article
Fisheries
Yeke Wang, Feng Chen, Jun He, Jun Chen, Ge Xue, Yan Zhao, Yanghui Peng, Ping Xie
Summary: This study reveals significant differences in the structure and protein composition of the egg envelopes of common carp adhesive eggs and grass carp semi-buoyant eggs, with common carp eggs having a thicker egg envelope and adhesive layer. Proteomic changes in common carp adhesive eggs after fertilization suggest a role in cell and fish egg morphology maintenance. Proteins upregulated in common carp adhesive eggs after fertilization, such as coagulation factor XIII A, zona pellucida proteins, and cystatin, may play a role in egg envelope hardening and embryonic development protection. Additionally, higher levels of alpha-2-macroglobulin-like protein in fertilized common carp eggs indicate potential for effective immune defense.
Article
Biochemistry & Molecular Biology
Lejeune Clara, Cornu David, Sago Laila, Redeker Virginie, Virolle Marie-Joelle
Summary: In this study, the growth and transcriptional/regulatory protein profiles of Streptomyces coelicolor and Streptomyces lividans were compared under conditions of phosphate limitation or proficiency. It was found that these two strains differ significantly in their ability to produce bioactive specialized metabolites. The study also revealed that S. coelicolor is subjected to nitrogen stress, which is closely linked to cell wall stress and osmotic stress. The intensification of these stresses leads to an increase in antibiotic biosynthesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemical Research Methods
Josie A. Christopher, Aikaterini Geladaki, Charlotte S. Dawson, Owen L. Vennard, Kathryn S. Lilley
Summary: The internal environment of cells is crowded with molecules, requiring spatial organization through subcellular compartmentalization. These compartments provide specific conditions for molecules to carry out their biological functions, and molecules traffic between these compartments. Aberrant localization of proteins or RNA species is associated with various pathological conditions. Differential expression studies often overlook the importance of subcellular information, and subcellular transcriptomics and proteomics data do not always overlap, highlighting their complementary nature.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Immunology
Maxime Lepretre, Nicole Faury, Amelie Segarra, Stephane Claverol, Lionel Degremont, Melissa Palos-Ladeiro, Jean Armengaud, Tristan Renault, Benjamin Morga
Summary: This study focused on experimental infection of Pacific oyster spat with ostreid herpesvirus type 1 (OsHV-1) and identified viral proteins with potential immunomodulatory functions, as well as key proteins in the host immune response against the viral infection. The results suggest that OsHV-1 may manipulate the host immune response, leading to weakening and death of Pacific oysters, while also highlighting the importance of proteins involved in RNA interference and interferon-like pathways for antiviral defenses in Pacific oysters.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Jinpeng Zou, Liang Yang, Yuhong Li, Mingxin Piao, Yaxing Li, Nan Yao, Xiaohong Zhang, Qian Zhang, Guanghui Hu, Deguang Yang, Zecheng Zuo
Summary: The yield of maize is significantly affected by low temperature, especially chilling stress during the seedling stage. This study investigated the protein changes underlying the chilling stress response in maize seedlings using physiological, proteomics, and hormone assays. The results identified signaling proteins and pathways involved in chilling stress, with the ABA pathway playing a key role in the chilling resistance of the tolerant maize line KR701. The catalogued chilling-responsive proteins provide a valuable resource for further research and maize breeding.
Article
Biochemistry & Molecular Biology
Ahmed Hammad Mirza, Liujuan Cui, Shuyan Zhang, Pingsheng Liu
Summary: Brown adipose tissue (BAT) contains lipid droplet-anchored mitochondria (LDAM) which are functional across different temperatures, with comparative proteomics analysis revealing differential protein expressions between LDAM and cytoplasmic mitochondria (CM) at different temperatures, indicating a preorganized and functional organelle complex.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2021)