Article
Veterinary Sciences
Haeseung Lee, Dongmi Kwak
Summary: This study investigated the infection rate of G. duodenalis in wild boars by comparing assemblages through PCR amplification of specific genes. The results showed that 20.4% of the 612 tested samples were positive for G. duodenalis, with the highest infection rate in the central region and in autumn. Phylogenetic analysis revealed three genetic assemblages, with A and B showing 100% identity with Giardia sequences isolated from humans and farmed pigs in Korea and Japan. Continuous management and monitoring of this pathogen are necessary to prevent transmission and protect animal and human health.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Immunology
Matthew H. Seabolt, Dawn M. Roellig, Konstantinos T. Konstantinidis
Summary: This study investigates the sub-assemblages of the parasitic protozoan Giardia duodenalis using genomic data and coalescent techniques, identifying two new clades and demonstrating their distinctiveness in terms of host adaptation and population structuring. The study also proposes the formal classification of sub-assemblage AII as a new species, Giardia hominis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Ozlem Orunc Kilinc, Adnan Ayan, Burcak Aslan Celik, Ozgur Yasar Celik, Nazmi Yuksek, Gurkan Akyildiz, Fatma Ertas Oguz
Summary: This study investigated the molecular characterization of G. duodenalis in buffaloes in the Van region in Turkiye. The results showed that buffaloes are an important reservoir for waterborne and foodborne giardiasis, with assemblage E and assemblage B isolates.
Article
Veterinary Sciences
Sang-Ik Oh, Suk-Han Jung, Han-Kyoung Lee, Changyong Choe, Tai-Young Hur, Kyoung-Min So
Summary: Giardia duodenalis infection was found in 5.6% of Korean native calves, with higher prevalence in calves aged >= 1 month and experiencing watery diarrhea. The majority of positive samples belonged to assemblage E, while a few belonged to zoonotic assemblage A, emphasizing the importance of continuous surveillance of genetic mutations in G. duodenalis.
VETERINARY SCIENCES
(2021)
Article
Veterinary Sciences
Zhao-Jun Heng, Jian-Fa Yang, Xin-Yan Xie, Cui-Rong Xu, Jun-Rong Chen, Jun Ma, Jun-Jun He, Hua-Ming Mao
Summary: This study investigated the occurrence and genetic diversity of Giardia duodenalis in Holstein cattle in Yunnan Province, China. The results showed a high infection rate in preweaned calves, with assemblage E being the predominant group. Mixed infection of assemblages A and E was also detected in three samples.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Review
Infectious Diseases
Xueping Zhang, Jiaming Dan, Liqin Wang, Haifeng Liu, Ziyao Zhou, Xiaoping Ma, Zhihua Ren, Hualin Fu, Yi Geng, Yan Luo, Yue Xie, Guangneng Peng, Zhijun Zhong
Summary: This study analyzed the genetic diversity and structure of G. duodenalis assemblage E in Chinese dairy cattle, revealing high haplotype diversity and low nucleotide diversity. The results also showed low genetic differentiation and frequent genetic communication among populations, with genetic variation within populations higher than among populations. Overall, the findings suggest that the G. duodenalis assemblage E population in Chinese dairy cattle has experienced demographic expansion.
INFECTION GENETICS AND EVOLUTION
(2021)
Article
Parasitology
Ya-Fei Song, Meng-Jie Chu, Fei Huang, Yang Liu, Hui-Hong Lu, Si-Ang Li, Shu-Yan Wang
Summary: Giardia duodenalis, a common intestinal protozoan, was found to have a prevalence of 3.59% in pigs from Guangxi Zhuang Autonomous Region. Multilocus genotyping analysis revealed the presence of A1 genotype. These findings provide important data for the prevention and control of G. duodenalis infections in pigs and humans in the region.
Article
Immunology
Dongfang Li, Han Deng, Yaxin Zheng, Hongyan Zhang, Sen Wang, Lan He, Junlong Zhao
Summary: This study investigated the infection rates and species/genotypes of Cryptosporidium spp. and G. duodenalis in pigs in Hubei, China. The results showed the presence of both parasites in intensive farms, posing a potential public health risk.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Parasitology
Josephine S. Y. Hublin, Jenny G. Maloney, Nadja S. George, Aleksey Molokin, Jason E. Lombard, Natalie J. Urie, Chelsey B. Shivley, Monica Santin
Summary: Giardia duodenalis is a common parasitic cause of gastrointestinal illness worldwide. Conventional molecular methods underestimate the occurrence of mixed infections in cattle. Next generation amplicon sequencing demonstrates superior sensitivity in detecting mixed infections.
VETERINARY PARASITOLOGY
(2022)
Review
Veterinary Sciences
Ali Taghipour, Mitra Sharbatkhori, Farideh Tohidi, Mohammad R. Ghanbari, Panagiotis Karanis, Meysam Olfatifar, Hamidreza Majidiani, Sasan Khazaei, Saeed Bahadory, Ehsan Javanmard
Summary: This study reports on the global distribution of G. duodenalis in cattle and evaluates the prevalence, risk factors, and genetic characterization of its infection. The results show that G. duodenalis infection is widespread among cattle and is associated with diarrhea and pre-weaned calves.
PREVENTIVE VETERINARY MEDICINE
(2022)
Article
Immunology
Chi Sun Yun, Bo-Youn Moon, Kichan Lee, Su Min Kang, Bok-Kyung Ku, Mi-Hye Hwang
Summary: This study aimed to detect and identify subspecies of gastrointestinal protozoa parasites in cats from South Korea. The results showed a high rate of infection with cat-specific Cryptosporidium and Cystoisospora, which are associated with living conditions and diarrhea symptoms in South Korean cats. Zoonotic and other animal-specific subtype of protozoan parasites have also been detected in cat feces.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Public, Environmental & Occupational Health
Piotr Solarczyk, Miroslawa Dabert, Alain C. Frantz, Natalia Osten-Sacken, Artur Trzebny, Agnieszka Wojtkowiak-Giera, Mike Heddergott
Summary: The study in Germany and Luxembourg revealed that raccoons carry a zoonotic genotype of Giardia duodenalis, posing a threat to public health in Europe and other regions where this species is common and lives in close association with humans.
ZOONOSES AND PUBLIC HEALTH
(2021)
Article
Public, Environmental & Occupational Health
Yuzhen Sui, Xiangqian Zhang, Haidong Wang, Fuchang Yu, Liping Zheng, Yunan Guo, Ying Lu, Minghui Chen, Bukang Wang, Hongyu Dai, Fang Liu, Junqiang Li, Haiju Dong, Chao Tong, Longxian Zhang
Summary: This study detected the infection of Giardia duodenalis in asymptomatic pet dogs in Zhengzhou, China, and investigated the relationship between gut microbiota and fecal characteristics. The results showed that the transmission of zoonotic giardiasis between humans and pet dogs is rare in Zhengzhou, and the use of Lactobacillus spp. as a potential probiotic agent could improve intestinal health in dogs and humans by treating G. duodenalis.
Review
Public, Environmental & Occupational Health
Sara Kalavani, Sara Matin, Vahid Rahmanian, Ahmad Meshkin, Ali Taghipour, Amir Abdoli
Summary: Giardia duodenalis is a major cause of diarrhea in children, and a systematic review and meta-analysis of studies on Asian children found a pooled prevalence of G. duodenalis infection at 15.1%. The highest and lowest pooled prevalence values were found in Tajikistan (26.4%) and China (0.6%) respectively. Male children had a higher prevalence of infection compared to females. The findings highlight the need for prevention and control measures for Giardiasis, especially in Asian countries with high prevalence rates.
INTERNATIONAL HEALTH
(2023)
Article
Immunology
Hong-Li Geng, Wei-Lan Yan, Jian-Min Wang, Jin-Xin Meng, Miao Zhang, Ji-Xin Zhao, Kai-Meng Shang, Jing Liu, Wen-Hua Liu
Summary: This study investigated the epidemiology of Giardia duodenum in sheep and goats in China and found a total prevalence rate of 7%. The infection rate in sheep and goats over 12 months was higher than in those under 12 months, and the highest prevalence was observed in summer. Geographical location, genotype, and climate were identified as potential risk factors for giardiasis prevalence. It is recommended to strengthen management measures and policies to reduce the incidence rate and economic loss in China's animal husbandry.
MICROBIAL PATHOGENESIS
(2023)
Article
Microbiology
Welmoed van Loon, Clara Bergmann, Felix Habarugira, Costanza Tacoli, Darius Savelsberg, Rafael Oliveira, Djibril Mbarushimana, Jules Ndoli, Augustin Sendegeya, Claude Bayingana, Frank P. Mockenhaupt
Summary: The study found an association between Plasmodium falciparum multidrug resistance-1 gene (pfmdr1) polymorphisms and altered antimalarial susceptibility, with a significant increase in the prevalence of the wild-type allele N86 and the wild-type combination NYD in recent years. The molecular data suggests a pattern shift in the pfmdr1 gene associated with artemether-lumefantrine resistance.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Lisa J. Kruger, Julian A. F. Klein, Frank Tobian, Mary Gaeddert, Federica Lainati, Sarah Klemm, Paul Schnitzler, Ralf Bartenschlager, Berati Cerikan, Christopher J. Neufeldt, Olga Nikolai, Andreas K. Lindner, Frank P. Mockenhaupt, Joachim Seybold, Terry C. Jones, Victor M. Corman, Nira R. Pollock, Britta Knorr, Andreas Welker, Margaretha de Vos, Jilian A. Sacks, Claudia M. Denkinger
Summary: The study confirmed that the microfluidic immunofluorescence LumiraDx (TM) assay has good sensitivity and specificity for SARS-CoV-2 detection. The assay also demonstrated favorable ease-of-use, making it a promising tool for pandemic control.
Article
Environmental Sciences
Sebastian Ruf, Franziska Hommes, Welmoed van Loon, Joachim Seybold, Tobias Kurth, Marcus A. Mall, Frank P. Mockenhaupt, Stefanie Theuring
Summary: The study investigated an outbreak in a Berlin kindergarten in November and December 2020 and found that viral spread within the facility mainly occurred through kindergarten staff, while children primarily transmitted infections within their families. To prevent outbreaks, strict adherence to hygiene rules, staff vaccinations against SARS-CoV-2, and immediate reaction to suspected cases by quarantining and frequent testing are reasonable measures.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Medicine, General & Internal
Lisa J. Krueger, Amilcar Tanuri, Andreas K. Lindner, Mary Gaeddert, Lisa Koppel, Frank Tobian, Lukas E. Brummer, Julian A. F. Klein, Federica Lainati, Paul Schnitzler, Olga Nikolai, Frank P. Mockenhaupt, Joachim Seybold, Victor M. Corman, Terence C. Jones, Christian Drosten, Claudius Gottschalk, Stefan F. Weber, Stephan Weber, Orlando C. Ferreira, Diana Mariani, Erika Ramos dos Santos Nascimento, Terezinha M. Pereira Pinto Castineiras, Rafael Mello Galliez, Debora Souza Faffe, Isabela de Carvalho Leitao, Claudia dos Santos Rodrigues, Thiago Silva Frauches, Keity J. Chagas Vilela Nocchi, Natalia Martins Feitosa, Sabrina Santana Ribeiro, Nira R. Pollock, Britta Knorr, Andreas Welker, Margaretha de Vos, JilianA Sacks, Stefano Ongarello, Claudia M. Denkinger
Summary: This study evaluated the clinical performance and ease-of-use of seven SARS-CoV-2 antigen-detecting rapid diagnostic tests (Ag-RDTs). The results showed high sensitivity and specificity of the Ag-RDTs, especially in early-stage disease and individuals with high viral loads. The tests from Mologic, Bionote, and Standard Q demonstrated good usability.
Letter
Microbiology
Andreas K. Lindner, Lisa J. Krueger, Olga Nikolai, Julian A. F. Klein, Heike Roessig, Paul Schnitzler, Victor M. Corman, Terry C. Jones, Frank Tobian, Mary Gaeddert, Susen Burock, Jilian A. Sacks, Joachim Seybold, Frank P. Mockenhaupt, Claudia M. Denkinger
MICROBIOLOGY SPECTRUM
(2022)
Article
Medicine, General & Internal
Rashid Mansoor, Robert J. Commons, Nicholas M. Douglas, Benjamin Abuaku, Jane Achan, Ishag Adam, George O. Adjei, Martin Adjuik, Bereket H. Alemayehu, Richard Allan, Elizabeth N. Allen, Anupkumar R. Anvikar, Emmanuel Arinaitwe, Elizabeth A. Ashley, Hazel Ashurst, Puji B. S. Asih, Nathan Bakyaita, Hubert Barennes, Karen Barnes, Leonardo Basco, Quique Bassat, Elisabeth Baudin, David J. Bell, Delia Bethell, Anders Bjorkman, Caroline Boulton, Teun Bousema, Philippe Brasseur, Hasifa Bukirwa, Rebekah Burrow, Verena Carrara, Michel Cot, Umberto D'Alessandro, Debashish Das, Sabyasachi Das, Timothy M. E. Davis, Meghna Desai, Abdoulaye A. Djimde, Arjen M. Dondorp, Grant Dorsey, Chris J. Drakeley, Stephan Duparc, Emmanuelle Espie, Jean-Francois Etard, Catherine Falade, Jean Francois Faucher, Scott Filler, Carole Fogg, Mark Fukuda, Oumar Gaye, Blaise Genton, Awab Ghulam Rahim, Julius Gilayeneh, Raquel Gonzalez, Rebecca F. Grais, Francesco Grandesso, Brian Greenwood, Anastasia Grivoyannis, Christoph Hatz, Eva Maria Hodel, Georgina S. Humphreys, Jimee Hwang, Deus Ishengoma, Elizabeth Juma, S. Patrick Kachur, Piet A. Kager, Erasmus Kamugisha, Moses R. Kamya, Corine Karema, Kassoum Kayentao, Adama Kazienga, Jean-Rene Kiechel, Poul-Erik Kofoed, Kwadwo Koram, Peter G. Kremsner, David G. Lalloo, Moses Laman, Sue J. Lee, Bertrand Lell, Amelia W. Maiga, Andreas Martensson, Mayfong Mayxay, Wilfred Mbacham, Rose McGready, Herve Menan, Didier Menard, Frank Mockenhaupt, Brioni R. Moore, Olaf Muller, Alain Nahum, Jean-Louis Ndiaye, Paul N. Newton, Billy E. Ngasala, Frederic Nikiema, Akindeh M. Nji, Harald Noedl, Francois Nosten, Bernhards R. Ogutu, Olusola Ojurongbe, Lyda Osorio, Jean-Bosco Ouedraogo, Seth Owusu-Agyei, Anil Pareek, Louis K. Penali, Patrice Piola, Mateusz Plucinski, Zul Premji, Michael Ramharter, Caitlin L. Richmond, Lars Rombo, Philip J. Rosenthal, Sam Salman, Albert Same-Ekobo, Carol Sibley, Sodiomon B. Sirima, Frank M. Smithuis, Fabrice A. Some, Sarah G. Staedke, Peter Starzengruber, Nathalie Strub-Wourgaft, Inge Sutanto, Todd D. Swarthout, Din Syafruddin, Ambrose O. Talisuna, Walter R. Taylor, Emmanuel A. Temu, Julie Thwing, Halidou Tinto, Emiliana Tjitra, Offianan A. Toure, T. Hien Tran, Johan Ursing, Innocent Valea, Giovanni Valentini, Michele van Vugt, Lorenz von Seidlein, Stephen A. Ward, Vincent Were, Nicholas J. White, Charles J. Woodrow, William Yavo, Adoke Yeka, Issaka Zongo, Julie A. Simpson, Philippe J. Guerin, Kasia Stepniewska, Ric N. Price, Cally Roper
Summary: In patients with uncomplicated P. falciparum malaria, the nadir hemoglobin occurs 2 days after starting treatment. Factors associated with moderately severe anemia on day 7 in Africa and Asia include severe anemia at baseline, young age, high parasitemia, and delayed parasite clearance. In Asia, artemisinin-based treatments are associated with a greater risk of anemia during recovery compared to non-artemisinin-based treatments.
Letter
Medicine, General & Internal
Samipa Pudasaini, Kira Louisa Boldt, Jennifer Hitzek, Linus Moeckel, Anna Slagman, Stefanie Theuring, Frank Mockenhaupt, Martin Moeckel
DEUTSCHES ARZTEBLATT INTERNATIONAL
(2022)
Article
Infectious Diseases
Sophia Kindzierski, Welmoed van Loon, Stefanie Theuring, Franziska Hommes, Eberhard Thombansen, Malik Boettcher, Harald Matthes, Heike Roessig, David Weiger, Christof Wiesmann, Tobias Kurth, Valerie Kirchberger, Joachim Seybold, Frank P. Mockenhaupt, Maximilian Gertler
Summary: The prevalence of SARS-CoV-2 infection was assessed in teachers and preschool educators, with higher odds of infection found in preschool educators compared to teachers. The private environment was identified as a relevant source of infection for educational staff in 2020.
Article
Public, Environmental & Occupational Health
Andreas K. Lindner, Franziska Hommes, Olga Nikolai, Gabriela Equihua Martinez, Berna Guerer, Renate Krueger, Rasmus Leistner, Dennis Nurjadi, Frank P. Mockenhaupt, Philipp Zanger
Summary: PVL-positive S. aureus skin and soft tissue infections have a significant impact on patients' quality of life and mental health. However, physicians involved in pre-treatment often lack knowledge about the PVL toxin. Increased awareness for early diagnosis and treatment of this neglected disease is necessary.
JOURNAL OF TRAVEL MEDICINE
(2022)
Article
Infectious Diseases
Carol A. Abidha, Yaw Ampem Amoako, Richard King Nyamekye, George Bedu-Addo, Florian Grziwotz, Frank P. Mockenhaupt, Arndt Telschow, Ina Danquah
Summary: This study aimed to investigate the susceptibility of adults with diabetes mellitus (DM) in malaria-endemic areas to Plasmodium infection. The results showed that fasting blood glucose (FBG) was causally affected by parasite density, and daily ambient temperature also affected parasite density. Longer observation periods and larger samples are needed to confirm these findings and determine the direction of causality.
Article
Environmental Sciences
Stefanie Theuring, Welmoed van Loon, Franziska Hommes, Norma Bethke, Marcus A. Mall, Tobias Kurth, Joachim Seybold, Frank P. Mockenhaupt
Summary: The COVID-19 pandemic has had a negative impact on the psychosocial wellbeing of students in Berlin, Germany. Anxiety symptoms and fear of infection increased, while social contacts and health-related quality of life decreased. Factors such as gender, age, school attendance, household income, and presence of adults with anxiety symptoms were associated with anxiety. Fear of infection and low HRQoL were also linked to anxiety.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Microbiology
Lisa J. Krueger, Andreas K. Lindner, Mary Gaeddert, Frank Tobian, Julian Klein, Salome Steinke, Federica Lainati, Paul Schnitzler, Olga Nikolai, Frank P. Mockenhaupt, Joachim Seybold, Victor M. Corman, Terry C. Jones, Nira R. Pollock, Britta Knorr, Andreas Welker, Stephan Weber, Nandini Sethurarnan, Jayanthi Swaminathan, Hilda Solomon, Ajay Padmanaban, Ma Thirunarayan, L. Prabakaran, Margaretha de Vos, Stefano Ongarello, Jilian A. Sacks, Camille Escadafal, Claudia M. Denkinger
Summary: Our manufacturer-independent prospective study assessed the clinical performance of participants with presumed SARS-CoV-2 infection at three study sites in two countries. We evaluated the accuracy overall and in predefined subgroups based on C-T values and symptom duration. The SureStatus diagnostic test showed high sensitivity, especially in the first few days after symptom onset and when C-T values were low. The system usability and ease-of-use assessment provided valuable insights for the widespread use of SureStatus in point-of-care settings. The high sensitivity demonstrated by the evaluated Ag-RDT during the early phase of infection supports its role in public health screening.
MICROBIOLOGY SPECTRUM
(2022)
Article
Endocrinology & Metabolism
Felix P. Chilunga, Pleun S. Schwerzel, Karlijn A. C. Meeks, Erik Beune, Silver Bahendeka, Frank Mockenhaupt, Kerstin Klipstein-Grobusch, Charles Agyemang
Summary: Psychosocial stress is associated with type 2 diabetes and glycaemic control among Ghanaians, especially in men. Negative life events are negatively correlated with type 2 diabetes, while perceived discrimination is positively correlated with type 2 diabetes.
Article
Pediatrics
Stefanie Theuring, Mascha Kern, Franziska Hommes, Marcus A. A. Mall, Joachim Seybold, Frank P. P. Mockenhaupt, Toivo Glatz, Tobias Kurth
Summary: During the COVID-19 pandemic, children and adolescents worldwide have been disproportionately affected in their psychological health and wellbeing. A cohort study in Germany found that anxiety symptoms among school children decreased from June to August 2021, but increased again in September 2021. Factors such as gender, school type, household education and income level, and COVID-19 vaccination were significantly associated with reporting anxiety symptoms.
CHILD AND ADOLESCENT PSYCHIATRY AND MENTAL HEALTH
(2023)
Article
Immunology
Welmoed van Loon, Rafael Oliveira, Clara Bergmann, Felix Habarugira, Jules Ndoli, Augustin Sendegeya, Claude Bayingana, Frank P. Mockenhaupt
Summary: The study documented artemisinin resistance in Plasmodium falciparum patient isolates from Rwanda carrying K13 R561H, A675V, and C469F mutations, indicating the presence of artemisinin resistance in the region.
EMERGING INFECTIOUS DISEASES
(2022)