Article
Multidisciplinary Sciences
Qing-xin Yu, Jiao-chen Wang, Jun-fei Liu, Lu-xia Ye, Yi-qing Guo, Hai-hong Zheng
Summary: This study investigated the role of ADRM1 in bladder cancer through bioinformatics analysis and immunohistochemical analysis. The results showed that high ADRM1 expression was associated with worse overall survival, enrichment in immune-related pathways, and a positive correlation with immune checkpoints. In the tumor microenvironment, high ADRM1 expression was associated with increased infiltration of CD8+ T cells and macrophages. Furthermore, patients with low ADRM1 expression were found to be sensitive to certain chemotherapy drugs. These findings highlight the prognostic significance of ADRM1 in bladder cancer and its potential as a biomarker and therapeutic target.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Wonhee Han, Youngmu Koo, Leila Chaieb, Byeong-Rak Keum, Jin-Kwan Han
Summary: This study identifies UCHL5 as a negative regulator of Wnt signaling and reveals its important role in the regulation of Axin1 protein. The study shows that UCHL5 is required for both the stabilization and the polymerization of Axin1 proteins.
SCIENTIFIC REPORTS
(2022)
Review
Genetics & Heredity
Yosuk Min, Hong-Beom Park, Kwang-Hyun Baek, Sohyun Hwang
Summary: In ovarian cancer, the survival rate is much higher for early stages than for advanced stages. However, most patients are diagnosed in the advanced stages and often experience recurrence. To address this, new biomarkers for early diagnosis and treatment are needed. This review focuses on deubiquitinating enzymes and their regulated substrates in ovarian cancer cells, which could aid in the discovery of biomarkers and therapeutic candidates.
Article
Parasitology
Shu Wei Yan, Yuan Yuan Hu, Yan Yan Song, Hua Nan Ren, Jia Ming Shen, Ruo Dan Liu, Shao Rong Long, Peng Jiang, Jing Cui, Zhong Quan Wang
Summary: TsCX, a crucial protease in Trichinella spiralis, promotes larval invasion of intestinal epithelial cells and is located throughout the worm body, potentially serving as a target for vaccines against enteral stages of the parasite.
VETERINARY PARASITOLOGY
(2021)
Review
Cell Biology
Yi Xiong, Chao Yu, Qianting Zhang
Summary: This review discusses the functions of ubiquitin proteasome system (UPS) components in mammalian spermatogenesis, including the composition of proteasome isoforms at each stage, the specificity and associated degradation events of each isoform, E3 ubiquitin ligases mediating protein ubiquitination, and the deubiquitinases involved in spermatogenesis and male fertility. Exploring the UPS machineries in spermatogenesis provides insights into protein dynamics during male germ cell production and sheds light on the etiology and pathogenesis of human male infertility.
Review
Biochemistry & Molecular Biology
Karen A. Sap, Karlijne W. Geijtenbeek, Sabine Schipper-Krom, Arzu Tugce Guler, Eric A. Reits
Summary: Huntington's disease (HD) is a neurodegenerative disorder caused by a CAG repeat expansion in the HTT gene, resulting in the accumulation and toxicity of mutant HTT (mHTT) protein. The ubiquitin-proteasome system (UPS) is involved in clearing mHTT proteins, but the polyQ expansion in mHTT affects its interaction with ubiquitinating enzymes and targeting for degradation. Various ubiquitin-modifying enzymes have been identified that can improve mHTT turnover and overall homeostasis, providing potential targets for therapeutic intervention in HD.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Kheerthana Duraivelan, Sagarika Dash, Dibyendu Samanta
Summary: Nectins, a family of cell surface glycoproteins, mediate cell-cell adhesion and signaling pathways, with specific heterophilic interactions dictating their physiological relevance.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Su Yeon Kim, Ji-young Lee, Yun-jung Cho, Kwan Hoon Jo, Eun Sook Kim, Je Ho Han, Kwang-Hyun Baek, Sung-dae Moon
Summary: This study found that USP37 is a specific deubiquitinating enzyme for CDC73, and the two proteins interact through specific domains, suggesting that USP37 plays an important role in the stability of CDC73 in HPT-JT syndrome.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Parasitology
Jie Zeng, Ru Zhang, Kai Ning Ma, Lu Lu Han, Shu Wei Yan, Ruo Dan Liu, Xi Zhang, Zhong Quan Wang, Jing Cui
Summary: TsAPP is a novel Trichinella spiralis aminopeptidase P (TsAPP) that hydrolyzes polypeptides and plays an important role in the invasion process.
EXPERIMENTAL PARASITOLOGY
(2022)
Article
Parasitology
Anna Zawistowska-Deniziak, Justyna Bien-Kalinowska, Katarzyna Basalaj
Summary: This study investigated the impact of direct contact of different stages of T. spiralis on the polarization of human THP-1 macrophages, revealing significant increases in levels of pro-inflammatory cytokines like IL-1 beta and TNF alpha in adult parasite and newborn larvae (NBL). Additionally, muscle larvae excretory-secretory products were found to significantly suppress the inflammatory response while enhancing anti-inflammatory IL-10 levels, suggesting anti-inflammatory potential.
PARASITOLOGY RESEARCH
(2021)
Article
Multidisciplinary Sciences
Xiuxiu Lu, Venkata R. Sabbasani, Vasty Osei-Amponsa, Christine N. Evans, Julianna C. King, Sergey G. Tarasov, Marzena Dyba, Sudipto Das, King C. Chan, Charles D. Schwieters, Sulbha Choudhari, Caroline Fromont, Yongmei Zhao, Bao Tran, Xiang Chen, Hiroshi Matsuo, Thorkell Andresson, Raj Chari, Rolf E. Swenson, Nadya I. Tarasova, Kylie J. Walters
Summary: The authors have identified and characterized XL5, a lead compound that binds to the N-terminal Pru domain of human Rpn13 (hRpn13), solved the NMR structure of XL5-ligated hRpn13 Pru, and developed XL5-PROTACs that preferentially target an identified hRpn13 Pru fragment present in multiple myeloma cells.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Ji Ma, Shudong Ma, Ying Zhang, Yi Shen, Lei Huang, Tianhao Lu, Lu Wang, Yunhan Wen, Zhenhua Ding
Summary: Depletion of kinectin1 (KTN1) inhibits tumorigenesis of cutaneous squamous cell carcinoma (cSCC) by reducing epidermal growth factor receptor (EGFR) protein levels. The underlying mechanism involves KTN1 knockdown promoting EGFR degradation in cSCC cells through the ubiquitin-proteasome system. KTN1 depletion also leads to tumor suppressor functions mediated by CCDC40, PSMA1, and ADRM1, providing novel insights into EGFR degradation in cSCC.
CELL DEATH & DISEASE
(2021)
Article
Geriatrics & Gerontology
Jerome Teuliere, Charles Bernard, Eduardo Corel, Francois-Joseph Lapointe, Johannes Martens, Philippe Lopez, Eric Bapteste
Summary: The genetic roots of ageing and longevity variations are not well understood. Comparative analyses have identified evolutionarily conserved pathways associated with longevity or ageing. This study focused on Opisthokonta species to analyze protein interactions related to ageing or longevity, providing comprehensive understanding of the evolution of ageing and longevity pathways. The study identified key antagonistic regulators of ageing/longevity and predicted novel evolutionarily conserved longevity/ageing-related proteins in humans, which could serve as potential targets for anti-ageing treatments.
Article
Multidisciplinary Sciences
Shuwen Zhang, Shitao Zou, Deyao Yin, Lihong Zhao, Daniel Finley, Zhaolong Wu, Youdong Mao
Summary: This study presents high-resolution cryo-electron microscopy structures of human USP14 in complex with the 26S proteasome, revealing two pathways of proteasome state transitions induced by USP14. The findings also highlight the dynamic USP14-ATPase interactions that decouple ATPase activity from deubiquitylation and introduce regulatory checkpoints in the proteasome function. These results provide insights into the complete functional cycle of the USP14-regulated proteasome and have implications for the development of USP14-targeted therapies.
Review
Cell Biology
Non-Nuoc Tran, Byung-Hoon Lee
Summary: Amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) are neurodegenerative diseases associated with the abnormal aggregation of TDP-43. The ubiquitinating and deubiquitinating pathways may modulate the pathogenicity and toxicity of TDP-43 through mechanisms such as protein stability and translocation. Understanding these mechanisms could lead to the development of targeted therapies for ALS and FTD.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Editorial Material
Parasitology
Murray E. Selkirk, Richard E. Davis, Kleoniki Gounaris, Rick M. Maizels
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2018)
Article
Genetics & Heredity
Silvana Rosic, Rachel Amouroux, Cristina E. Requena, Ana Gomes, Max Emperle, Toni Beltran, Jayant K. Rane, Sarah Linnett, Murray E. Selkirk, Philipp H. Schiffer, Allison J. Bancroft, Richard K. Grencis, Albert Jeltsch, Petra Hajkova, Peter Sarkies
Article
Multidisciplinary Sciences
Marion Rolot, Annette M. Dougall, Alisha Chetty, Justine Javaux, Ting Chen, Xue Xiao, Benedicte Machiels, Murray E. Selkirk, Rick M. Maizels, Cornelis Hokke, Olivier Denis, Frank Brombacher, Alain Vanderplasschen, Laurent Gillet, William G. C. Horsnell, Benjamin G. Dewals
NATURE COMMUNICATIONS
(2018)
Article
Parasitology
Peter J. Taylor, Jana Hagen, Farid N. Faruqu, Khuloud T. Al-Jamal, Bonnie Quigley, Morgan Beeby, Murray E. Selkirk, Peter Sarkies
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2020)
Article
Parasitology
Rita Berkachy, Danielle J. Smyth, Corinna Schnoeller, Yvonne Harcus, Rick M. Maizels, Murray E. Selkirk, Kleoniki Gounaris
Summary: The study characterized five apyrases secreted by the natural parasite of mice, Heligmosomoides polygyrus, which showed strict calcium dependence and a broad substrate specificity for nucleoside tri- and diphosphates. Immunization with an apyrase cocktail provided partial protection against reinfection in mice, indicating the potential role of nematode secreted apyrases in modulating immune response.
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2021)
Article
Allergy
Matthew Darby, Luke B. Roberts, Claire Mackowiak, Alisha Chetty, Sasha Tinelli, Corinna Schnoeller, Valerie Quesniaux, Sylvie Berrard, Dieudonnee Togbe, Murray E. Selkirk, Bernard Ryffel, William G. C. Horsnell
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2021)
Article
Infectious Diseases
Mellina T. Srey, Alessia Taccogna, Yelena Oksov, Sara Lustigman, Pei-Yi Tai, John Acord, Murray E. Selkirk, Tracey J. Lamb, David B. Guiliano
PLOS NEGLECTED TROPICAL DISEASES
(2020)
Article
Infectious Diseases
Anja de Lange, Ulrich Fabien Prodjinotho, Hayley Tomes, Jana Hagen, Brittany-Amber Jacobs, Katherine Smith, William Horsnell, Chummy Sikasunge, Dorit Hockman, Murray E. Selkirk, Clarissa Prazeres da Costa, Joseph Valentino Raimondo
PLOS NEGLECTED TROPICAL DISEASES
(2020)
Article
Microbiology
Jana Hagen, Peter Sarkies, Murray E. Selkirk
Summary: The use of a pseudotyped viral vector has successfully enabled genetic manipulation of parasitic nematodes, including gene silencing and expression of exogenous genes. This method holds promise for advancing research on the complex biology of nematode parasites and identifying new targets for infection control.
Article
Immunology
Luke B. Roberts, Corinna Schnoeller, Rita Berkachy, Matthew Darby, Jamie Pillaye, Menno J. Oudhoff, Naveen Parmar, Claire Mackowiak, Delphine Sedda, Valerie Quesniaux, Bernhard Ryffel, Rachel Vaux, Kleoniki Gounaris, Sylvie Berrard, David R. Withers, William G. C. Horsnell, Murray E. Selkirk
Summary: ILC2s are shown to synthesize and release acetylcholine during parasitic nematode infection. Exposure to extracts of certain substances or factors in vivo can promote the synthesis of acetylcholine by ILC2s. The ACh derived from ILC2s plays a functional role in expanding ILC2s for maximal induction of type 2 immunity.
SCIENCE IMMUNOLOGY
(2021)
Article
Immunology
Luke B. Roberts, Geraldine M. Jowett, Emily Read, Tomas Zabinski, Rita Berkachy, Murray E. Selkirk, Ian Jackson, Umar Niazi, Nelomi Anandagoda, Masatake Araki, Kimi Araki, Jagath Kasturiarachchi, Chela James, Tariq Enver, Rachael Nimmo, Rita Reis, Jane K. Howard, Joana F. Neves, Graham M. Lord
Summary: miR-142 plays a critical role in the homeostasis and function of ILC2s, deficiency of miR-142 leads to biased development of ILC2 progenitors and significant changes in phenotype, affecting ILC2-mediated immune responses.
JOURNAL OF IMMUNOLOGY
(2021)
Correction
Respiratory System
E. Culerier, D. Togbe, I. Maillet, M. Le Bert, M. Selkirk, W. Horsnell, V. Quesniaux, B. Ryffel
REVUE DES MALADIES RESPIRATOIRES
(2022)
Article
Immunology
Luke B. Roberts, Rita Berkachy, Madina Wane, Dhiren F. Patel, Corinna Schnoeller, Graham M. Lord, Kleoniki Gounaris, Bernhard Ryffel, Valerie Quesniaux, Matthew Darby, William G. C. Horsnell, Murray E. Selkirk
Summary: Acetylcholine plays an important role in regulating immune responses and is associated with the development of various diseases. This study demonstrates that Acetylcholine derived from group 2 innate lymphoid cells (ILC2s) limits excessive pulmonary neutrophilic inflammation and enhances eosinophilia. However, depletion of Acetylcholine inhibits ILC2 activation and suppresses eosinophilic inflammation. Acetylcholine also regulates macrophage phenotype in allergic airway inflammation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biology
Alice Brown, Murray E. Selkirk, Peter Sarkies
Summary: This study investigated the secretion mechanism and stability of miRNAs in the parasitic nematode Trichinella spiralis. Proteomics analysis identified two RNA binding proteins secreted by T. spiralis larvae. One of these proteins showed selective and sequence-specific binding to miRNA, while the other protein exhibited non-specific binding. These findings provide insights into the possible mechanism of miRNA secretion in T. spiralis and contribute to our understanding of extracellular miRNA biology.
Meeting Abstract
Immunology
Luke B. Roberts, Geraldine M. Jowett, Emily Read, Rita Berkachy, Murray E. Selkirk, Umar Niazi, Nelomi Anandagoda, Chela James, Rachael Nimmo, Jane K. Howard, Joana F. Neves, Graham M. Lord
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)