Article
Biochemistry & Molecular Biology
Ivan Belyaev, Alessandra Marolda, Jan-Philipp Praetorius, Arjun Sarkar, Anna Medyukhina, Kerstin Huenniger, Oliver Kurzai, Marc Thilo Figge
Summary: Rapid identification of pathogens is essential for early diagnosis and treatment of life-threatening bloodstream infections. Researchers have successfully differentiated different infection scenarios and accurately identified pathogen-free samples by observing the behavior and morphodynamics of neutrophils. This study has significant implications for the development of new molecular diagnostic tools.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Biochemical Research Methods
Feline E. Dijkgraaf, Mireille Toebes, Mark Hoogenboezem, Marjolijn Mertz, David W. Vredevoogd, Tiago R. Matos, Marcel B. M. Teunissen, Rosalie M. Luiten, Ton N. Schumacher
Summary: This ex vivo imaging approach allows for the study of immune cell behavior in human skin and can be applied to research on immune cell function in murine skin. The ability to track the spatiotemporal behavior of immune cells in skin, including their response to immune stimuli, provides a platform for investigating physiological immune cell behavior as well as immune cell behavior in skin diseases.
Article
Immunology
Maria Adelaida Gomez, Ashton Trey Belew, Adriana Navas, Mariana Rosales-Chilama, Julieth Murillo, Laura A. L. Dillon, Theresa A. Alexander, Alvaro Martinez-Valencia, Najib M. El-Sayed
Summary: This study investigates the early host determinants of healing and non-healing responses in human cutaneous leishmaniasis caused by Leishmania (Viannia) panamensis. Transcriptomic profiling of peripheral blood mononuclear cells from CL patients showed that the parasite modulates adaptive immune cell functions early on, impacting immune responses beyond innate and phagocytic functions.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Takako Tabata, Matthew Petitt, Julia Li, Xiaoyuan Chi, Wei Chen, Irina Yurgelonis, Sabine Wellnitz, Simon Bredl, Tiago Vicente, Xinzhen Yang, Philip R. Dormitzer, Lenore Pereira
Summary: Human cytomegalovirus (HCMV), the leading viral cause of congenital disease and permanent birth defects, could potentially be prevented by a prophylactic vaccine targeting the pentameric complex and gB, as they elicit neutralizing antibodies.
Article
Chemistry, Physical
Mohammad Zhiani, Saeid Barzi, Ali Ahmadi, Francesco Vizza, Hussein Gharibi, Amirhossein Azhari
Summary: This study presents a new design for AGFCs and demonstrates their feasibility and high performance in the presence of glucose fuel and oxygen supply from different sources.
JOURNAL OF POWER SOURCES
(2022)
Article
Biochemistry & Molecular Biology
Giuseppe Annunziata, Xavier Capo, Maria Magdalena Quetglas-Llabres, Margalida Monserrat-Mesquida, Silvia Tejada, Josep A. Tur, Roberto Ciampaglia, Fabrizia Guerra, Maria Maisto, Gian Carlo Tenore, Ettore Novellino, Antoni Sureda
Summary: The study evaluated a novel nutraceutical formulation, Taurisolo(R), based on polyphenolic extract from Aglianico cultivar grape pomace, showing significant antioxidant activity and the ability to activate nuclear factors, reduce reactive oxygen species levels, decrease pro-inflammatory gene expression, and induce anti-inflammatory cytokine expression.
Article
Engineering, Environmental
Sandra Ballesteros, Josefa Domenech, Antonia Velazquez, Ricard Marcos, Alba Hernandez
Summary: The biomedical applications of graphene-based nanomaterials have seen significant growth in recent years, with important implications for the interaction between GBN and blood cells triggering immune responses. The study used an innovative approach to study the effects of GBN on the secretome and showed potential impacts on cell growth, providing relevant data for biomedical use of GBN in patients.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Ophthalmology
Noor Alantary, Wayne Heaselgrave, Scott Hau
Summary: This study correlated the different forms of Acanthamoeba on ex vivo confocal microscopy (EVCM) with in vivo confocal microscopy (IVCM) and cultured positive cases of Acanthamoeba keratitis. EVCM and IVCM showed good correlation, and cyst morphology changed with imaging depth.
BRITISH JOURNAL OF OPHTHALMOLOGY
(2022)
Article
Environmental Sciences
Vaclav Tvrdy, Patricia Dias, Iveta Nejmanova, Alejandro Carazo, Eduard Jirkovsky, Jana Pourova, Jaka Fadraersada, Monika Moravcova, Lucija Peterlin Masic, Marija Sollner Dolenc, Premysl Mladenka
Summary: The human population is regularly exposed to bisphenols, which are potentially toxic. This study aimed to test the effects of 14 bisphenols on the cardiovascular system and compare their effects in different settings. Most of the tested bisphenols could relax rat aorta, but their potency varied significantly.
Article
Engineering, Electrical & Electronic
Tristan Putzeys, Anastasiya Starovoyt, Nicolas Verhaert, Michael Wubbenhorst
Summary: This study utilized dielectric relaxation spectroscopy to investigate the molecular relaxation properties of human perilymph, finding that the geometry of white blood cells in perilymph is related to dielectric absorption. Interestingly, perilymph obtained from fixed temporal bones matches fresh perilymph better than when obtained from fresh-frozen sources, indicating the importance of well-preserved cell geometry.
IEEE TRANSACTIONS ON DIELECTRICS AND ELECTRICAL INSULATION
(2021)
Article
Orthopedics
I. Hellberg, V. -P. Karjalainen, M. A. J. Finnilä, E. Jonsson, A. Turkiewicz, P. Önnerfjord, V. Hughes, J. Tjörnstrand, M. Englund, S. Saarakkala
Summary: This study proposed a micro-computed tomography (mu CT) based 3D analysis of meniscal calcifications ex vivo, including a new grading system. The results showed that mu CT images can effectively depict meniscal calcifications, and the new grading system can accurately measure the severity of meniscal calcification. Two different patterns of degeneration were observed.
OSTEOARTHRITIS AND CARTILAGE
(2023)
Article
Developmental Biology
Rachel R. Zabel, Rodolfo R. Favaro, Tanja Groten, Paul Brownbill, Sarah Jones
Summary: Studying pregnancy pathologies using the ex vivo placenta perfusion model can improve understanding of the diseases, enhance diagnostic and treatment strategies. The unique and safe nature of the model makes it a valuable tool for exploring maternal-fetal transfer, hemodynamics, and testing therapeutic agents.
Article
Cell Biology
Tatjana Reif, Gerhard Dyckhoff, Ralph Hohenberger, Carl-Christian Kolbe, Henning Gruell, Florian Klein, Eicke Latz, Bettina Stolp, Oliver T. Fackler
Summary: Polymorphonuclear neutrophils (PMNs) can effectively suppress HIV-1 replication in target cells and tissues through an unconventional mechanism that does not involve degranulation, formation of neutrophil extracellular traps, or integrin-dependent cell communication.
CELL REPORTS MEDICINE
(2021)
Article
Infectious Diseases
Minh Patrick Le, Lucile Pencole, Gilles Peytavin, Florian Bouchet-Crivat, Laurent Mandelbrot
Summary: In this study, it was found that doravirine crosses the placenta and accumulates within it, potentially providing a prophylaxis for vertical HIV transmission. Further investigation is needed to determine the safety and efficacy of this new antiretroviral agent in pregnancy.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Cell & Tissue Engineering
Daisuke Araki, Jian Fei Fu, Heather Huntsman, Stefan Cordes, Fayaz Seifuddin, Luigi J. Alvarado, Patali S. Cheruku, Ayla Cash, Javier Traba, Yuesheng Li, Mehdi Pirooznia, Richard H. Smith, Andre Larochelle
Summary: Activation of NOTCH signaling using engineered DELTA-like ligand (DXI) under low oxygen tension culture conditions in human adult HSPCs can limit ER stress, increase the frequency of LTR-HSCs significantly, and help maintain undifferentiated phenotypes in cultured cells.
Article
Biochemical Research Methods
Inmaculada Moreno, Mercedes Dominguez, Alfredo Torano
JOURNAL OF IMMUNOLOGICAL METHODS
(2020)
Article
Veterinary Sciences
A. Roy, J. A. Infantes-Lorenzo, M. Dominguez, I. Moreno, M. Perez, N. Garcia, T. Garcia-Seco, J. Alvarez, B. Romero, C. Gortazar, L. de Juan, L. Dominguez, J. Bezos
RESEARCH IN VETERINARY SCIENCE
(2020)
Article
Multidisciplinary Sciences
Jordi Martinez-Guijosa, Beatriz Romero, Jose Antonio Infantes-Lorenzo, Elena Diez, Mariana Boadella, Ana Balseiro, Miguel Veiga, David Navarro, Inmaculada Moreno, Javier Ferreres, Mercedes Dominguez, Cesar Fernandez, Lucas Dominguez, Christian Gortazar
Article
Veterinary Sciences
Jose Antonio Infantes-Lorenzo, Christian Gortazar, Lucas Dominguez, Marta Munoz-Mendoza, Mercedes Dominguez, Ana Balseiro
RESEARCH IN VETERINARY SCIENCE
(2020)
Article
Infectious Diseases
Isabel G. Fernandez-de-Mera, Francisco J. Rodriguez Del-Rio, Jose de la Fuente, Marta Perez-Sancho, Dolores Hervas, Inmaculada Moreno, Mercedes Dominguez, Lucas Dominguez, Christian Gortazar
Summary: The investigation in a rural community in Spain with a high prevalence of COVID-19 found that 12% of samples collected from environmental surfaces tested positive for SARS-CoV-2 RNA. While this highlights the potential for environmental transmission, it is not yet recommended as a standard approach for COVID-19 epidemiology.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Biochemical Research Methods
Alfredo Torano, Inmaculada Moreno, Jose Antonio Infantes, Mercedes Dominguez
Summary: The study presents a competitive inhibition ELISA method for measuring monoclonal antibody inhibition constants, suggesting similar results to surface plasmon resonance. The method is simple, sensitive, and reproducible, making it a useful tool for calculating mAb inhibition constants.
JOURNAL OF IMMUNOLOGICAL METHODS
(2021)
Article
Veterinary Sciences
Javier Ortega, Jose A. Infantes-Lorenzo, Javier Bezos, Alvaro Roy, Lucia de Juan, Beatriz Romero, Inmaculada Moreno, Alberto Gomez-Buendia, Irene Agullo-Ros, Lucas Dominguez, Mercedes Dominguez
Summary: The study evaluated the performance of an in-house ELISA for TB diagnosis in goats using oral fluid samples, showing that the test is highly specific but has limited sensitivity. However, it performs better in detecting infected animals with severe lesions.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Veterinary Sciences
Maria Victoria Ortega-Garcia, Francisco Javier Salguero, Nerea Garcia, Mercedes Dominguez, Inmaculada Moreno, Alexis Berrocal
Summary: This study describes five cases of equine cutaneous leishmaniosis in Costa Rica, with the etiological diagnosis based on the presence of the parasite in the lesions. The results showed granulomatous inflammation within the dermis and emphasized the importance of Leishmania spp. as a causative agent of equine cutaneous disease in the New World.
VETERINARY MEDICINE AND SCIENCE
(2021)
Article
Infectious Diseases
Sandra Barroso-Arevalo, Alberto Barneto, Angel Manuel Ramos, Belen Rivera, Rocio Sanchez, Lidia Sanchez-Morales, Marta Perez-Sancho, Aranzazu Buendia, Elisa Ferreras, Juan Carlos Ortiz-Menendez, Inmaculada Moreno, Consuelo Serres, Carmen Vela, Maria Angeles Risalde, Lucas Dominguez, Jose M. Sanchez-Vizcaino
Summary: The study in Spain shows that pets are susceptible to SARS-CoV-2 infection in natural conditions, but at a low level. Only 0.79% of the total animals analyzed were positive for viral infection. Infected humans remain the main source of transmission, but including animals in COVID-19 surveillance is still recommended.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Immunology
Jaime Larraga, Pedro J. Alcolea, Ana M. Alonso, Luis T. C. Martins, Inmaculada Moreno, Mercedes Dominguez, Vicente Larraga
Summary: Leishmania parasites cause significant morbidity and mortality in many developing countries. Researchers have identified a vaccine candidate, LiUBC1, which shows partial protection against L. infantum infection in a Syrian hamster model.
Article
Veterinary Sciences
J. Ortega, J. A. Infantes-Lorenzo, A. Roy, L. de Juan, B. Romero, I Moreno, M. Dominguez, L. Dominguez, J. Bezos
Summary: This study analyzed goat milk samples to investigate the changes in antibody levels and its relationship with serological test results. The study also examined the effects of lactation stage, booster effect, and freeze-thaw cycles on milk samples.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Veterinary Sciences
J. A. Infantes-Lorenzo, B. Romero, A. Rodriguez-Bertos, A. Roy, J. Ortega, L. de Juan, I. Moreno, M. Dominguez, L. Dominguez, J. Bezos
BMC VETERINARY RESEARCH
(2020)
Article
Multidisciplinary Sciences
A. I. Olias-Molero, I. Moreno, M. J. Corral, M. D. Jimenez-Anton, M. J. Day, M. Dominguez, J. M. Alunda
SCIENTIFIC REPORTS
(2020)