Article
Cell Biology
Lei Sang, Rui Dong, Rui Liu, Qinggang Hao, Weiyu Bai, Jianwei Sun
Summary: Our study reveals that NHR-14/HNF4 alpha cooperates with CEP-1/p53 to regulate DNA damage-induced apoptosis.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Biochemistry & Molecular Biology
Antonia Piazzesi, Yiru Wang, Joshua Jackson, Lena Wischhof, Viktoria Zeisler-Diehl, Enzo Scifo, Ina Oganezova, Thorben Hoffmann, Pablo Gomez Martin, Fabio Bertan, Chester J. J. Wrobel, Frank C. Schroeder, Dan Ehninger, Kristian Haendler, Joachim L. Schultze, Lukas Schreiber, Gerhild Van Echten-Deckert, Pierluigi Nicotera, Daniele Bano
Summary: Mitochondrial dysfunction can be influenced by enhanced metabolic flexibility, which can extend the lifespan of mitochondrial mutants. CEST-2.2, a carboxylesterase mainly localized in the intestine, has been found to stimulate the survival of mitochondrial deficient animals. Through transcriptome and lipidome analysis, it has been shown that CEST-2.2 promotes lipid metabolism and fatty acid beta-oxidation, enhancing the respiratory capacity of mitochondria and extending the lifespan of mutant nematodes.
Article
Multidisciplinary Sciences
Daniel F. Midkiff, Javier Huayta, James D. Lichty, Joseph P. Crapster, Adriana San-Miguel
Summary: In this study, we used microfluidic technologies and image processing to perform high-throughput automated screening for short-lived mutants based on protein aggregation. We identified lifespan mutants by screening for accelerated protein aggregation through quantitative analysis of fluorescently labeled aggregates, avoiding the need for conditional sterilization or manual separation.
Article
Cell Biology
Nan Xin, Jenni Durieux, Chunxia Yang, Suzanne Wolff, Hyun-Eui Kim, Andrew Dillin
Summary: The mitochondrial unfolded protein response (UPRmt) maintains mitochondrial proteostasis by regulating the transcription factor ATFS-1. After UPRmt activation, ATFS-1 enters the nucleus to regulate UPRmt genes. The study found that despite reduced mitochondrial membrane potential, mitochondrial import increases during UPRmt activation, and an intact import machinery is essential for UPRmt-mediated lifespan extension.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Cell Biology
Qian Zhang, Zihao Wang, Wenfeng Zhang, Qingbo Wen, Xinyu Li, Jun Zhou, Xueying Wu, Yongqing Guo, Yangli Liu, Changshuo Wei, Wenfeng Qian, Ye Tian
Summary: Parental stress memories can be passed on to offspring by elevating mitochondrial DNA levels, inducing UPRmt in the next generation to better cope with challenges.
NATURE CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Siqi Jiang, Na Deng, Bisheng Zheng, Tong Li, Rui Hai Liu
Summary: Rhodiola extract was found to significantly extend the lifespan of C. elegans and enhance its stress resistance by regulating gene expression and promoting protein translocation.
Article
Biochemical Research Methods
Nguyen Phuoc Long, Hyung Min Kim
Summary: This study investigated the metabolic consequences of mitochondrial DNA mutations in C. elegans using an untargeted metabolomics approach. Three mutant strains (clk-1, mev-1, and phb-2) exhibited significant metabolic differences compared to the wildtype worms, with distinct patterns of metabolic alterations observed in each mutant strain. The metabolome changes appeared to be correlated with the lifespan of C. elegans, with clk-1 and mev-1 strains showing different metabolomes despite having opposite lifespan phenotypes. These findings shed light on the metabolic effects of mitochondrial genetic variants and may contribute to a better understanding of mitochondrial disease mechanisms.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Multidisciplinary Sciences
Christine E. Robbins, Bhumil Patel, Danielle L. Sawyer, Barrie Wilkinson, Brian K. Kennedy, Mark A. McCormick
Summary: Deletion of genes encoding ribosomal proteins extends lifespan in yeast by increasing translation of the transcription factor Gcn4. Inhibitors of cytosolic tRNA synthetase and mitochondrial tRNA synthetase also extend the lifespan of C. elegans, possibly acting through the same downstream pathway.
Article
Biochemistry & Molecular Biology
Shiling Feng, Chunyan Zhang, Tao Chen, Lijun Zhou, Yan Huang, Ming Yuan, Tian Li, Chunbang Ding
Summary: The study found that OLE significantly prolongs the lifespan of Caenorhabditis elegans and increases their resistance to lethal heat shock and oxidative stress. OLE regulates stress resistance and lifespan extension in nematodes through the activation of the IIS and SKN-1/Nrf2 signaling pathways.
Article
Genetics & Heredity
Yangli Tian, Qiaoju Kang, Xuemeng Shi, Yuan Wang, Nali Zhang, Huan Ye, Qifeng Xu, Tao Xu, Rongying Zhang
Summary: SNX-3 plays a crucial role in organizing tubular endosomes for efficient recycling of CIE cargoes by competing with EEA-1 for binding to early endosomes. However, the mechanism by which SNX-3 couples cargo capture and membrane remodeling in the absence of the retromer trimer complex remains to be fully understood. Our findings provide insight into the sorting and trafficking of CIE pathways.
Article
Cell Biology
Mengnan Zhu, Fukang Teng, Na Li, Li Zhang, Shuxian Zhang, Fan Xu, Jing Shao, Haipeng Sun, Huanhu Zhu
Summary: This study identified a lipid biosynthesis pathway that mediates amino acid sensing in the intestine and neuronal mTORC1, affecting development by regulating protein translation and ribosomal biogenesis. Findings suggest a similar mechanism may operate in mammals, providing new insights into the role of amino acid sensing mechanisms in animals.
DEVELOPMENTAL CELL
(2021)
Article
Cell Biology
Li-Tzu Chen, Chih-Ta Lin, Liang-Yi Lin, Jiun-Min Hsu, Yu-Chun Wu, Chun-Liang Pan
Summary: In C. elegans, loss of neuronal fzo-1/mito-fusin induces nonautonomous UPRmt through multiple neurotransmitters and neurohormones. This triggers nonautonomous mitochondrial fragmentation, requiring genes involved in autophagy and mitophagy. The systemic activation of UPRmt and mitochondrial fragmentation improves resistance to pathogenic Pseudomonas infection in C. elegans.
DEVELOPMENTAL CELL
(2021)
Article
Chemistry, Medicinal
Jing-Shan Wu, Chun-Guo Lin, Chang-Long Jin, Yan-Xia Zhou, Ying-Xiu Li
Summary: The marine bacterium Planococcus maritimu ML1206 was found to have antioxidant properties and could enhance the ability of nematodes to resist oxidative stress and extend their lifespan. These findings suggest that ML1206 has potential anti-aging and antioxidant functions and could be used in aquaculture and as a dietary supplement.
Article
Multidisciplinary Sciences
Peng Wang, Qiushi Wang, Lianwan Chen, Zheng Cao, Hailian Zhao, Ruibao Su, Ning Wang, Xiaojing Ma, Jin Shan, Xinyan Chen, Qi Zhang, Baochen Du, Zhiheng Yuan, Yanmei Zhao, Xiaorong Zhang, Xuejiang Guo, Yuanchao Xue, Long Miao
Summary: This study identifies a germline-specific mitochondrial RBP AMG-1, which is crucial for spermatogenesis in Caenorhabditis elegans. AMG-1 mainly binds to mtDNA-encoded ribosomal RNA and regulates mitochondrial protein homeostasis. Additionally, SLRP-1 interacts with AMG-1 to regulate germline development and reproductive success.
Article
Cell Biology
Yangli Liu, Jun Zhou, Ning Zhang, Xueying Wu, Qian Zhang, Wenfeng Zhang, Xinyu Li, Ye Tian
Summary: Mitochondrial perturbations in neurons can regulate organism-wide mitochondrial homeostasis and adaptability by activating the unfolded protein response in the intestine. This regulation involves the coordinated action of GPCR and its downstream signaling in sensory neurons.
DEVELOPMENTAL CELL
(2022)
Article
Medicine, Research & Experimental
Cecile Otten, Jessica Knox, Gwenola Boulday, Mathias Eymery, Marta Haniszewski, Martin Neuenschwander, Silke Radetzki, Ingo Vogt, Kristina Haehn, Coralie De Luca, Cecile Cardoso, Sabri Hamad, Carla Igual Gil, Peter Roy, Corinne Albiges-Rizo, Eva Faurobert, Jens P. von Kries, Monica Campillos, Elisabeth Tournier-Lasserve, W. Brent Derry, Salim Abdelilah-Seyfried
EMBO MOLECULAR MEDICINE
(2018)
Article
Cell Biology
Benjamin Lant, Swati Pal, Eric Michael Chapman, Bin Yu, Daniel Witvliet, Soo Choi, Lisa Zhao, Corinne Albiges-Rizo, Eva Faurobert, W. Brent Derry
Article
Biochemistry & Molecular Biology
Anh T. Tran, Eric M. Chapman, Mathieu N. Flamand, Bin Yu, Samuel J. Krempel, Thomas F. Duchaine, Matthew Eroglu, W. Brent Derry
CELL DEATH AND DIFFERENTIATION
(2019)
Article
Multidisciplinary Sciences
Eric M. Chapman, Benjamin Lant, Yota Ohashi, Bin Yu, Michael Schertzberg, Christopher Go, Deepika Dogra, Janne Koskimaki, Romuald Girard, Yan Li, Andrew G. Fraser, Issam A. Awad, Salim Abdelilah-Seyfried, Anne-Claude Gingras, W. Brent Derry
NATURE COMMUNICATIONS
(2019)
Article
Biochemistry & Molecular Biology
Claire E. Richardson, Callista Yee, Kang Shen
Review
Biochemistry & Molecular Biology
Salim Abdelilah-Seyfried, Elisabeth Tournier-Lasserve, W. Brent Derry
TRENDS IN MOLECULAR MEDICINE
(2020)
Editorial Material
Biochemistry & Molecular Biology
W. Brent Derry
Summary: CRISPR is a prokaryotic immune surveillance system used by bacteria to defend against attackers by cleaving foreign nucleic acid fragments. The pioneers of CRISPR-Cas system were awarded the Nobel Prize and their technology is widely used in molecular biology laboratories worldwide.
Review
Oncology
Uswa Shahzad, Michael S. Taccone, Sachin A. Kumar, Hidehiro Okura, Stacey Krumholtz, Joji Ishida, Coco Mine, Kyle Gouveia, Julia Edgar, Christian Smith, Madeline Hayes, Xi Huang, W. Brent Derry, Michael D. Taylor, James T. Rutka
Summary: This review discusses the benefits of using non-murine species in cancer research, such as fruit flies, nematodes, and zebrafish, to study molecular processes related to cell development and cancer. These organisms have been found to share high genetic homology with humans and offer advantages such as shorter lifespans and suitability for high-throughput drug screening. The proposal suggests that utilizing these powerful screening tools and strategies can expedite discoveries in the field of cancer research and neuro-oncology.
Article
Cell Biology
Ceryl Tan, Miriam B. Ginzberg, Rachel Webster, Seshu Iyengar, Shixuan Liu, David Papadopoli, John Concannon, Yuan Wang, Douglas S. Auld, Jeremy L. Jenkins, Hannes Rost, Ivan Topisirovic, Andreas Hilfinger, W. Brent Derry, Nish Patel, Ran Kafri
Summary: This study demonstrates that cell size is determined by size checkpoints, and p38 and CDK4 cooperate to regulate cell size akin to a thermostat.
DEVELOPMENTAL CELL
(2021)
Article
Multidisciplinary Sciences
Aishwarya Subramanian, Mathew Hall, Huayun Hou, Marat Mufteev, Bin Yu, Kyoko E. Yuki, Haruka Nishimura, Anson Sathaseevan, Benjamin Lant, Beibei Zhai, James Ellis, Michael D. Wilson, Mads Daugaard, W. Brent Derry
Summary: This study identifies a conserved functional role of alternative polyadenylation in oncogenic Ras function, revealing the impact of cfim-1/CFIm25 on cell migration and the previously unknown regulator mrp-5/ABCC1 through experiments in Caenorhabditis elegans and human cells.
Article
Biology
Michael A. Q. Martinez, Angelina A. Mullarkey, Callista Yee, Chris Z. Zhao, Wan Zhang, Kang Shen, David Q. Matus
Summary: Development of the Caenorhabditis elegans reproductive tract is regulated by the anchor cell (AC). Transcription factors EGL-43, HLH-2, and NHR-67 play crucial roles in the regulation of AC invasion. Recent research has shown a mechanistic connection between EGL-43 loss and AC cell-cycle entry, suggesting that Notch signaling has mitogenic effects in the invasive AC. Using a novel co-expression system called AIDHB, researchers discovered that LIN-12 is not required for AC proliferation following loss of EGL-43.
Article
Biochemistry & Molecular Biology
Matthew Eroglu, Bin Yu, W. Brent Derry
Summary: Highly efficient insertion of deletions, substitutions, and small insertions (up to 150 bp) into the Caenorhabditis elegans genome using CRISPR/Cas9 has been made easier with the use of single-stranded oligonucleotide donors. However, inserting larger sequences such as fluorescent markers and functional domains remains challenging due to uncertainty and labor-intensive protocols. In this study, a simplified method for generating long single-stranded DNA (ssDNA) donors in CRISPR/Cas9 is presented, which shows significantly increased insertion frequency compared to double-stranded DNA (dsDNA) donors. This approach complements the existing CRISPR/Cas9 toolkit for C. elegans and enables efficient insertion of longer sequences with a simple and standardized protocol.
Article
Genetics & Heredity
Sarel J. Urso, Anson Sathaseevan, W. Brent Derry, Todd Lamitina
Summary: Maintenance of osmotic homeostasis is an important physiological process involving the upregulation of proteins that accumulate solutes called organic osmolytes. A genetic screen in Caenorhabditis elegans identified mutants with no induction of osmolyte biosynthesis gene expression (Nio mutants). The mutations were found in the genes cpf-2/CstF64 and symk-1/Symplekin, which are nuclear components of the mRNA cleavage and polyadenylation complex.
Editorial Material
Biochemistry & Molecular Biology
W. Brent Derry
Summary: Developmental programs are networks of signaling pathways that regulate the formation of tissues and organs during organismal development. Disruptions or activations of these programs can lead to diseases through abnormal growth, differentiation, or migration of cells. This collection of articles in The FEBS Journal highlights the role of deregulated signaling pathways in human diseases.
Article
Medicine, Research & Experimental
Janne Koskimaki, Romuald Girard, Yan Li, Laleh Saadat, Hussein A. Zeineddine, Rhonda Lightle, Thomas Moore, Sean Lyne, Kenneth Avner, Robert Shenkar, Ying Cao, Changbin Shi, Sean P. Polster, Dongdong Zhang, Julian Carrion-Penagos, Sharbel Romanos, Gregory Fonseca, Miguel A. Lopez-Ramirez, Eric M. Chapman, Evelyn Popiel, Alan T. Tang, Amy Akers, Pieter Faber, Jorge Andrade, Mark Ginsberg, W. Brent Derry, Mark L. Kahn, Douglas A. Marchuk, Issam A. Awad