Journal
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
Volume -, Issue 98, Pages -Publisher
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/52412
Keywords
Immunology; Issue 98; Heligmosomoides polygyrus; Helminth; Life Cycle; Excretory-Secretory Products; Immunology; Infection; Mouse
Categories
Funding
- Wellcome Trust through the Edinburgh Clinical Academic Track
- BBSRC
- American Asthma Foundation
- Asthma UK
- Rainin Foundation
- Asthma UK [SPD-2012-172] Funding Source: researchfish
- Biotechnology and Biological Sciences Research Council [1276234] Funding Source: researchfish
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Heligmosomoides polygyrus (formerly known as Nematospiroides dubius, and also referred to by some as H. bakeri) is a gastrointestinal helminth that employs multiple immunomodulatory mechanisms to establish chronic infection in mice and closely resembles prevalent human helminth infections. H. polygyrus has been studied extensively in the field of helminth-derived immune regulation and has been found to potently suppress experimental models of allergy and autoimmunity (both with active infection and isolated secreted products). The protocol described in this paper outlines management of the H. polygyrus life cycle for consistent production of L3 larvae, recovery of adult parasites, and collection of their excretory-secretory products (HES).
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