Article
Medicine, Research & Experimental
Mark A. Bryniarski, Ruben M. Sandoval, Donna M. Ruszaj, John Fraser-McArthur, Benjamin M. Yee, Rabi Yacoub, Lee D. Chaves, Silvia B. Campos-Bilderback, Bruce A. Molitoris, Marilyn E. Morris
Summary: Despite its importance in the pharmacokinetics of therapeutic proteins and peptides, the processes that occur following glomerular filtration are poorly understood. This study aimed to address this issue and develop a methodology to study the renal disposition of a therapeutic protein called exenatide. The results showed that exenatide is primarily cleared through glomerular filtration and subsequent renal catabolism.
MOLECULAR PHARMACEUTICS
(2023)
Review
Cell Biology
Nazli Alizadeh-Tabrizi, Stefan Hall, Christian Lehmann
Summary: Intravital microscopy is a unique imaging method that provides real-time insights into cellular functions without the need for tissue extraction. Despite specific challenges in lung imaging, advancements in surgical and optical techniques have enabled researchers to uncover mechanisms of leukocyte recruitment and function in models of pulmonary inflammation and infection.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biology
Iara Mota-Silva, Miguel A. R. B. Castanho, Ana Santos Silva-Herdade
Summary: Inflammation is crucial for organism protection and wound repair, but chronic inflammation can lead to microvasculature damage. Therefore, monitoring inflammation is important in testing potential therapeutics. Intravital microscopy (IVM) is a commonly used technique to monitor leukocyte trafficking in vivo and report systemic conditions. This study aims to investigate whether IVM can be performed using the ear lobe instead of the commonly used cremaster muscle. Results showed that the ear lobe protocol for IVM is efficient, non-invasive, more reliable, cost-effective and timesaving, making it a suitable alternative for future studies.
Review
Biotechnology & Applied Microbiology
Ravikumar Vaghela, Andreas Arkudas, Raymund E. Horch, Maximilian Hessenauer
Summary: Intravital microscopy (IVM) offers real-time imaging and tracking of cellular events. It plays a significant role in studying tissue regeneration processes and analyzing host reactions to implanted biomaterials.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kayle Dickson, Cassidy Scott, Hannah White, Juan Zhou, Melanie Kelly, Christian Lehmann
Summary: Beta-caryophyllene has been shown to have anti-inflammatory effects and potential antibacterial properties. This study investigated the effects of beta-caryophyllene in a murine model of urinary tract infection (UTI) and found that it significantly reduced bacterial burden, improved behavioral responses, and reduced inflammation in the bladder. This suggests that beta-caryophyllene could be a useful adjunct therapy for UTI management.
Article
Immunology
Wagdi Almishri, Rachelle P. Davis, Abdel-Aziz Shaheen, Mohammed O. Altonsy, Craig N. Jenne, Mark G. Swain
Summary: Mirtazapine treatment rapidly alters hepatic B cell populations, enhancing hepatic retention of CXCR3-expressing innate-like B cells that generate a more anti-inflammatory cytokine profile. Mirtazapine-induced hepatic B cell shifts could potentially represent a novel therapeutic approach to immune-mediated liver diseases characterized by B cell driven pathology.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Physiology
Patrick Arndt, Jan Sradnick, Hannah Kroeger, Stefan Holtzhausen, Friederike Kessel, Michael Gerlach, Vladimir Todorov, Christian Hugo
Summary: This study proposes the hypothesis of a local intra-extraglomerular regenerative feedback system for renin cells (RCN) and establishes a new quantifiable system for RCN responses. The study utilizes intravital microscopy and three-dimensional image processing algorithms to investigate the dynamic migration and repair process of RCN in individual glomeruli.
FRONTIERS IN PHYSIOLOGY
(2022)
Review
Endocrinology & Metabolism
Theja Bhamidipati, Manishekhar Kumar, Sumit S. S. Verma, Sujit K. K. Mohanty, Sedat Kacar, Diamond Reese, Michelle M. M. Martinez, Malgorzata M. M. Kamocka, Kenneth W. W. Dunn, Chandan K. K. Sen, Kanhaiya Singh
Summary: Type 2 diabetes mellitus causes peripheral vascular disease and its vascular complications may lead to organ dysfunction. This review discusses the importance of epigenetic processes such as DNA methylation and histone modifications in the development of diabetes complications.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Review
Cell Biology
Sofie Vandendriessche, Seppe Cambier, Paul Proost, Pedro E. Marques
Summary: The complement system plays a crucial role in controlling microorganisms and removing dead cells, while also stimulating adaptive immune responses and tissue regeneration. Complement receptors on leukocytes and parenchymal cells are essential for recognizing complement-derived molecules and promoting immune responses.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Immunology
Stefanie Wissmann, Bettina Stolp, Ana Marcos Jimenez, Jens Stein
Summary: This study reveals the interdependency of DOCK2 and p110 delta signaling pathways during the migration of naive B cells to CXCL13. p110 delta contributes mainly to CXCR5-mediated migration, while DOCK2 complements its role. Interference with both pathways completely abolishes B cell chemotaxis to CXCL13.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Alexandros Marios Sofias, Geir Bjorkoy, Jordi Ochando, Linda Sonstevold, Maria Hegvik, Catharina de Lange Davies, Olav Haraldseth, Twan Lammers, Willem J. M. Mulder, Sjoerd Hak
Summary: Active-targeting nanomedicine formulations exhibit complex in vivo behavior, with recent studies showing extensive uptake by circulating phagocytes targeting inflamed lesions. Mechanistically, the targeting of cRGD-NPs towards inflamed lesions is similar to NP accumulation in cancerous lesions. Circulating phagocytes engage cRGD-NPs and are homed to the inflamed endothelium, contributing to the extensive presence of cRGD-NP-positive phagocytes in angiogenic vessels. This study highlights the importance of detailed investigations into the in vivo behavior of nanoparticles for their potential as advanced therapeutic agents.
Letter
Oncology
Shrikant B. Mali
Summary: Intravital microscopy and optical coherency tomography are powerful imaging tools that have revolutionized cancer diagnosis and therapies. They allow for real-time observation of biological processes in vivo, providing insights into tumor behaviors. Recent developments in techniques for observing deep tissues have improved the detection of subcellular events and made these technologies indispensable in basic sciences and drug development.
Article
Biochemistry & Molecular Biology
Piper A. Robida, Alena P. Chumanevich, Alexa Orr Gandy, John W. Fuseler, Prakash Nagarkatti, Mitzi Nagarkatti, Carole A. Oskeritzian
Summary: The study suggests that in the pre-lesional phase of atopic dermatitis, elevated levels of ceramides lead to increased apoptosis of skin cells, which may be influenced by activated mast cells. The results reveal that the MC/Cer axis may be an essential pathogenic feature in the development of atopic dermatitis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Violaine Hubert, Ines Hristovska, Szilvia Karpati, Sarah Benkeder, Arindam Dey, Chloe Dumot, Camille Amaz, Naura Chounlamountri, Chantal Watrin, Jean-Christophe Comte, Fabien Chauveau, Emmanuel Brun, Patrice Marche, Frederic Lerouge, Stephane Parola, Yves Berthezene, Thomas Vorup-Jensen, Olivier Pascual, Marlene Wiart
Summary: This study proposes and validates a preclinical in vivo MRI tool to monitor neuroinflammation following ischemic stroke, based on injection of a novel multimodal nanoprobe designed for internalization by phagocytic cells. The study confirms efficient internalization of NanoGd by microglia and shows hypointense signals in the ischemic lesion using in vivo MRI with NanoGd injection in a mouse model. Additionally, the study demonstrates NanoGd internalization by activated phagocytic cells through both intravital microscopy and ex vivo analysis.
Review
Immunology
Eoin N. Blythe, Lynne C. Weaver, Arthur Brown, Gregory A. Dekaban
Summary: CD11d/CD18 is a recently discovered and poorly understood β2 integrin, playing a crucial role in localizing white blood cells to inflammation sites. Although its adhesive mechanisms and signaling pathways have been investigated, further research is needed to fully understand its functions.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Anke Liepelt, Alexander Wehr, Marlene Kohlhepp, Jana C. Mossanen, Karina Kreggenwinkel, Bernd Denecke, Ivan G. Costa, Tom Luedde, Christian Trautwein, Frank Tacke
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2019)
Article
Gastroenterology & Hepatology
Jana C. Mossanen, Marlene Kohlhepp, Alexander Wehr, Oliver Krenkel, Anke Liepelt, Anjali A. Roeth, Diana Moeckel, Felix Heymann, Twan Lammers, Nikolaus Gassler, Juliane Hermann, Joachim Jankowski, Ulf P. Neumann, Tom Luedde, Christian Trautwein, Frank Tacke
Article
Biochemistry & Molecular Biology
Junji Iwasaki, Mamdouh Afify, Christian Bleilevens, Uwe Klinge, Ralf Weiskirchen, Julia Steitz, Michael Vogt, Shintaro Yagi, Kazuyuki Nagai, Shinji Uemoto, Rene H. Tolba
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Engineering, Biomedical
Daniela F. Duarte Campos, Malena Rohde, Mitchell Ross, Parham Anvari, Andreas Blaeser, Michael Vogt, Claudia Panfil, Gary Hin-Fai Yam, Jodhbir S. Mehta, Horst Fischer, Peter Walter, Matthias Fuest
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2019)
Article
Cell Biology
Daniela F. Duarte Campos, Siyuan Zhang, Franziska Kreimendahl, Marius Koepf, Horst Fischer, Michael Vogt, Andreas Blaeser, Christian Apel, Marcella Esteves-Oliveira
CONNECTIVE TISSUE RESEARCH
(2020)
Letter
Gastroenterology & Hepatology
Jana C. Mossanen, Frank Tacke
Article
Multidisciplinary Sciences
Jana C. Mossanen, Tobias U. Jansen, Jessica Pracht, Anke Liepelt, Lukas Buendgens, Christian Stoppe, Andreas Goetzenich, Tim-Philipp Simon, Ruediger Autschbach, Gernot Marx, Frank Tacke
SCIENTIFIC REPORTS
(2020)
Article
Veterinary Sciences
Magdalena A. Czubala, Eva Eilles, Andreas Staubi, Natacha Ipseiz, Michael Vogt, Leonie Zieglowski, Lisa Ernst, Rene H. Tolba, Philip R. Taylor, Ralf Weiskirchen
Summary: The research team has proposed a digital platform called '3R Blackboard' to facilitate the sharing of surplus biological materials from animals, aiming to reduce the number of animals used. Pilot study has shown that this platform is highly effective, resulting in a significant reduction in the number of animals used, as well as resource saving, knowledge exchange, and establishment of new collaborations.
LABORATORY ANIMALS
(2022)
Article
Biotechnology & Applied Microbiology
Romain Lopez, Baoguo Li, Hadas Keren-Shaul, Pierre Boyeau, Merav Kedmi, David Pilzer, Adam Jelinski, Ido Yofe, Eyal David, Allon Wagner, Can Ergen, Yoseph Addadi, Ofra Golani, Franca Ronchese, Michael I. Jordan, Ido Amit, Nir Yosef
Summary: Most spatial transcriptomics technologies have resolution limitations. However, the DestVI method can provide higher-resolution analysis of cellular organization and gene expression changes in tissues.
NATURE BIOTECHNOLOGY
(2022)
Article
Instruments & Instrumentation
Federica De Lorenzi, Larissa Yokota Rizzo, Rasika Daware, Alessandro Motta, Maike Baues, Matthias Bartneck, Michael Vogt, Marc van Zandvoort, Leonard Kaps, Qizhi Hu, Marielle Thewissen, Luca Casettari, Cristianne J. F. Rijcken, Fabian Kiessling, Alexandros Marios Sofias, Twan Lammers
Summary: This study explores the use of cyclic arginine-glycine-aspartic acid (cRGD) peptide-decorated polymeric micelles for tumor-targeted drug delivery. The results show that decoration density, incubation time and temperature, and the expression levels of the alpha(v)beta(3) integrin receptor on target cells all influence the uptake of cRGD-decorated polymeric micelles by the target cells.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2023)
Review
Pharmacology & Pharmacy
Jeffrey Momoh, Dimitrios Kapsokalyvas, Michael Vogt, Sjoerd Hak, Fabian Kiessling, Marc van Zandvoort, Twan Lammers, Alexandros Marios Sofias
Summary: Intravital microscopy (IVM) has broad applications in various fields, including biology, physiology, immunology, and drug development. This review provides an overview of the modalities, methods, and model organisms used in IVM, as well as its applications in nanomedicine and biological process studies.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Article
Engineering, Biomedical
Ramin Nasehi, Ali T. Abdallah, Marcella Pantile, Carlo Zanon, Michael Vogt, Stephan Ruetten, Horst Fischer, Sanja Aveic
Summary: The lack of in vitro cell models is a challenge for discovering new molecular targets and therapeutics for pediatric bone metastatic disease. Using a beta-tricalcium phosphate (β-TCP) multicellular 3D in vitro bone microtissue model, the study successfully reconstitutes key phenotypic and transcriptional patterns of native metastatic tumor cells and enhances their stemness and proinvasive features. The geometry and chemical impact of β-TCP reveals a prometastatic landscape of the tumor microenvironment, providing insights for further analysis of the disease and preclinical target validations.
MATERIALS TODAY BIO
(2023)
Article
Materials Science, Biomaterials
Sanja Aveic, Simon Janssen, Ramin Nasehi, Max Seidelmann, Michael Vogt, Marcella Pantile, Stephan Ruetten, Horst Fischer
Summary: The geometry of synthetic beta-tricalcium-phosphate structures was found to impact the interaction between neuroblastoma tumor cells and stromal components. Growth conditions in a 3D environment are crucial for determining cell proliferation rates, with channeled 3D structures stimulating more cytokine production.
BIOMATERIALS SCIENCE
(2021)
Article
Gastroenterology & Hepatology
Felix Piecha, Jan-Michael Gaenssler, Sabine Jordan, Can Ergen, Harald Ittrich, Johannes Kluwe, Sven Pischke, Ansgar W. Lohse, Julian Schulze zur Wiesch
HEPATOLOGY COMMUNICATIONS
(2019)
Article
Gastroenterology & Hepatology
Matthias Bartneck, Peter L. Schrammen, Diana Moeckel, Olivier Govaere, Anke Liepelt, Oliver Krenkel, Can Ergen, Misti Vanette McCain, Dirk Eulberg, Tom Luedde, Christian Trautwein, Fabian Kiessling, Helen Reeves, Twan Lammers, Frank Tacke
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2019)