Journal
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
Volume -, Issue 98, Pages -Publisher
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/52354
Keywords
Bioengineering; Issue 98; Vascular surgery; Tissue Engineered Vessel; Surgical Technique; Bio-Engineering; Vascular Grafts; Implantation; Sheep; Large animal model; Carotid Artery; Anastomosis
Categories
Funding
- National Heart and Lung Institute [R01 HL086582]
- New York Stem Cell Science Fund (NYSTEM) [C024316]
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The development of Tissue Engineered Vessels (TEVs) is advanced by the ability to routinely and effectively implant TEVs (4-5 mm in diameter) into a large animal model. A step by-step protocol for inter-positional placement of the TEV and real-time digital assessment of the TEV and native carotid arteries is described here. In vivo monitoring is made possible by the implantation of flow probes, catheters and ultrasonic crystals (capable of recording dynamic diameter changes of implanted TEVs and native carotid arteries) at the time of surgery. Once implanted, researchers can calculate arterial blood flow patterns, invasive blood pressure and artery diameter yielding parameters such as pulse wave velocity, augmentation index, pulse pressures and compliance. Data acquisition is accomplished using a single computer program for analysis throughout the duration of the experiment. Such invaluable data provides insight into TEV matrix remodeling, its resemblance to native/sham controls and overall TEV performance in vivo.
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