Article
Oncology
Anson Ku, Jacob Fredsoe, Karina D. Sorensen, Michael Borre, Mikael Evander, Thomas Laurell, Hans Lilja, Yvonne Ceder
Summary: The development of an acoustic trapping technology allows for automatic enrichment of EVs from clinical samples, enabling robust and disease-discriminatory small RNA sequencing for diagnostic purposes.
FRONTIERS IN ONCOLOGY
(2021)
Article
Multidisciplinary Sciences
Gaolian Xu, Julien Reboud, Yunfei Guo, Hao Yang, Hongchen Gu, Chunhai Fan, Xiaohua Qian, Jonathan M. Cooper
Summary: Accelerating the design of nucleic acid amplification methods is challenging, but necessary for diagnosing diseases. This study presents a generalizable isothermal nucleic acid amplification methodology that can be applied to clinical settings, allowing for the detection of short miRNAs and difficult pathogenic targets.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Shiyi Wang, Ziwen Zong, Jianguo Xu, Bangben Yao, Zhou Xu, Li Yao, Wei Chen
Summary: In this study, a visual strategy for rapid and ultrasensitive detection of OTA was proposed by integrating primer-mediated exponential rolling circle amplification (P-ERCA) with a designed nucleic acid lateral flow strip (LFS). This method demonstrates highly sensitive detection of OTA and could be utilized as a universal method for detection of other chemical analytes.
ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Juxiang Liu, Xulong Hu, Yinxia Hu, Ping Chen, Hao Xu, Wenjun Hu, Yanteng Zhao, Ping Wu, Gang L. Liu
Summary: We present a low-cost, rapid, ultrasensitive, and convenient biosensing strategy for the detection of miR-21 using a nanoplasmonic array chip coupled with gold nanoparticles (AuNPs). This platform combines NanoSPR and LSPR effects, allowing real-time monitoring of subtle OD changes caused by variations in the dielectric constant during miRNA hybridization. The method has a broad detection range (100 aM to 1 mu M), a limit of detection of 1.85 aM, and single-base resolution for discriminating highly homologous miRNAs. It also exhibits high throughput characteristics, enabling simultaneous detection of 96 samples and offering more than 86.5 times enhancement in sensitivity compared to traditional biosensors.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Virology
Come J. Thieulent, Mariano Carossino, Laura Peak, Wendy Wolfson, Udeni B. R. Balasuriya
Summary: A panel of multiplex RT-qPCR assays has been developed and validated for the simultaneous detection of enteric viral infections, including SARS-CoV-2, in dogs and cats. The assays demonstrated high sensitivity and reliability.
Article
Biochemistry & Molecular Biology
Yuan Chen, Haibo Long, Tuizi Feng, Yueling Pei, Yanfang Sun, Xinchun Zhang
Summary: In this study, a novel primer-TaqMan probe set was developed for the identification and quantification of Meloidogyne enterolobii using qPCR and ddPCR technologies. Compared with qPCR, ddPCR exhibited better performance in detecting the nematodes concealed in field soil.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Fisheries
Yanmin Guo, Yahui Wang, Zhaobin Fan, Xianlin Zhao, Sven M. Bergmann, Hanxu Dong, Yuqi Jin, Dongli Sun, Qianyi Mai, Weiqiang Liu, Weiwei Zeng
Summary: A qPCR and real-time RAA assay were established for the detection of LMBV, with qPCR more suitable for quantitative analysis in the laboratory and real-time RAA more suitable as a point-of-care diagnostic tool for on-site detection and screening of LMBV under farm conditions.
JOURNAL OF FISH DISEASES
(2022)
Article
Chemistry, Multidisciplinary
Qian Zhang, Yun Han, Chen-Chen Li, Xiaoran Zou, Fei Ma, Chun-Yang Zhang
Summary: We have developed a dual-functional dumbbell probe-based fluorescent biosensor that can detect miRNAs in lung cancer cells and tissues through cascade amplification, and holds great potential in biomedical research and clinical diagnosis.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Yong Ju, Hyo Yong Kim, Jun Ki Ahn, Hyun Gyu Park
Summary: NASBA is an isothermal amplification technique for detecting RNA targets, but lacks sufficient efficiency. We developed NESBA by integrating a nicking and extension chain reaction system, which greatly enhances RNA amplification sensitivity through the design of specific primer sets.
Article
Chemistry, Analytical
Anton M. Solovjev, Ivan I. Galkin, Alexey V. Medved'ko, Olga Yu. Pletjushkina, Shulin Zhao, Ivan Yu. Sakharov
Summary: Heterogeneous and homogeneous-heterogeneous assays for miRNA-141 quantitation were developed using ICSDPR, with the former showing higher sensitivity. The assays demonstrated high specificity and successful detection of miRNA-141 in human cultured cells. The study further highlighted the intracellular content of miRNA-141 in different cell lines, showcasing the potential utility of these assays in miRNA research.
Article
Agriculture, Multidisciplinary
Shuai Zhi, Jinling Shen, Xingang Li, Yuan Jiang, Junxin Xue, Taisong Fang, Jin Xu, Xuan Wang, Yuhao Cao, Danting Yang, Zhiyuan Yao, Daniel Yu
Summary: A new RAA-exo-probe and RAA-CRISPR/Cas12a assays were developed for the rapid detection of Campylobacter jejuni in food samples. The assays showed high specificity and sensitivity, with a detection threshold lower than the traditional culture method. The simplicity and low equipment requirement make these assays suitable for field or poorly equipped labs.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Yajuan Dong, Xiuming Wu, Shenwei Li, Renfei Lu, Yingxue Li, Zhenzhou Wan, Jianru Qin, Guoying Yu, Xia Jin, Chiyu Zhang
Summary: This study compared the performance of 19 sets of primers in COVID-19 detection and identified 6 sets of primers with faster amplification. Among them, the Set-4 primer set had the highest positive detection rate of SARS-CoV-2.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Hye-Soon Song, Hyeon-Su Kim, Ji-Ye Kim, Yong-Kuk Kwon, Hye-Ryoung Kim
Summary: Newcastle disease (ND) is a highly pathogenic viral infection of poultry with significant economic impacts worldwide. Novel sets of visual reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays have been developed for the rapid detection and differentiation of NDV, showing potential for field or resource-limited environments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Thomas Leichtfried, Helga Reisenzein, Siegrid Steinkellner, Richard A. Gottsberger
Summary: The study found that slight modifications of the reverse transcription step in the real-time PCR assay can significantly improve the detection limit, especially for certain plant viruses. This discovery could enhance the sensitivity in detecting viroids and benefit regulatory outcomes for national plant protection organizations.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Chemistry, Analytical
Shan Huang, Bo Li, Pingping Mu, Wenqian Zhang, Yi Liu, Qi Xiao
Summary: A nitrogen-doped carbon dots (N-CDs)-based ratio fluorescence sensing strategy was developed for the highly sensitive and selective detection of microRNA-21 (miRNA-21). This approach demonstrated practical feasibility and excellent specificity for the analysis of miRNA-21 in HeLa cell lysates and human serum samples.
ANALYTICA CHIMICA ACTA
(2023)
Article
Endocrinology & Metabolism
Feifei Cheng, Andrea O. Luk, Mai Shi, Chuiguo Huang, Guozhi Jiang, Aimin Yang, Hongjiang Wu, Cadmon K. P. Lim, Claudia H. T. Tam, Baoqi Fan, Eric S. H. Lau, Alex C. W. Ng, Kwun Kiu Wong, Luke Carroll, Heung Man Lee, Alice P. Kong, Anthony C. Keech, Elaine Chow, Mugdha Joglekar, Stephen K. W. Tsui, Wing Yee So, Hon Cheong So, Anandwardhan A. Hardikar, Alicia J. Jenkins, Juliana C. N. Chan, Ronald C. W. Ma
Summary: This study found that shorter leukocyte telomere length is associated with an increased risk of glycemic progression in patients with type 2 diabetes. Shorter telomere length is independently associated with higher glycemic exposure during follow-up and a greater risk of diabetes progression. The findings suggest that telomere length may serve as a useful biomarker for monitoring glycemic progression in individuals with type 2 diabetes.
Article
Gastroenterology & Hepatology
Mugdha Joglekar, Subhshri Sahu, Wilson K. M. Wong, Sarang N. Satoor, Charlotte X. Dong, Ryan J. Farr, Michael D. Williams, Prapti Pandya, Gaurang Jhala, Sundy N. Y. Yang, Yi Vee Chew, Nicola Hetherington, Dhan Thiruchevlam, Sasikala Mitnala, Guduru Rao, Duvvuru Nageshwar Reddy, Thomas Loudovaris, Wayne J. Hawthorne, Andrew G. Elefanty, Vinay M. Joglekar, Edouard G. Stanley, David Martin, Helen E. Thomas, David Tosh, Louise T. Dalgaard, Anandwardhan A. Hardikar
Summary: This study reveals the presence of functional insulin-producing cells in the epithelial cells of the gallbladder, both during development and adulthood. Interestingly, these insulin-producing cells in the gallbladder are not targeted by autoimmune cells in a mouse model of type 1 diabetes. These findings highlight the evolutionary and developmental similarities between the gallbladder and the pancreas, and provide potential insights for type 1 diabetes therapy.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2022)
Article
Multidisciplinary Sciences
Ryan J. Farr, Christina L. Rootes, John Stenos, Chwan Hong Foo, Christopher Cowled, Cameron R. Stewart
Summary: Host biomarkers, specifically miRNAs, are being studied for their potential as tools for improved COVID-19 detection. This study analyzed the expression of miRNAs in anterior nasal tissues from COVID-19 patients and identified eight miRNAs with altered expression, with miR-142-3p showing the strongest upregulation. Machine learning analysis revealed a three-miRNA signature (miR-30c-2-3p, miR-628-3p, and miR-93-5p) that can independently classify COVID-19 cases with 100% accuracy. These findings provide further insight into the host miRNA response to SARS-CoV-2 infection and suggest potential biomarkers for improved COVID-19 detection.
Article
Medicine, General & Internal
Callum J. Hensman, Judith L. Gooley, Andrzej S. Januszewski, Melissa H. Lee, Richard J. Maclsaac, Raymond C. Boston, Glenn M. Ward, Alicia J. Jenkins
Summary: Insulin antibody levels are higher in adults with type 1 diabetes compared to those without diabetes. The levels are also found to be decreasing after islet cell transplantation.
INTERNAL MEDICINE JOURNAL
(2022)
Review
Medicine, General & Internal
Ehsan Alvandi, Wilson K. M. Wong, Mugdha Joglekar, Kevin J. Spring, Anandwardhan A. Hardikar
Summary: A systematic review and meta-analysis showed that lower fecal concentrations of short-chain fatty acids (SCFAs) are associated with higher risk and incidence of colorectal cancer (CRC).
Article
Medicine, General & Internal
Kwun Kiu Wong, Feifei Cheng, Cadmon K. P. Lim, Claudia H. T. Tam, Greg Tutino, Lai Yuk Yuen, Chi Chiu Wang, Yong Hou, Michael H. M. Chan, Chung Shun Ho, Mugdha V. Joglekar, Anandwardhan A. Hardikar, Alicia J. Jenkins, Boyd E. Metzger, William L. Lowe, Wing Hung Tam, Ronald C. W. Ma
Summary: The study investigates the relationship between maternal and offspring glucose metabolism with offspring leukocyte telomere length (LTL) in early life. The findings suggest that offspring sex is an important modifier of the disparate relationships in insulin production and response.
Correction
Endocrinology & Metabolism
Benjamin N. Rao, Nicola Quinn, Andrzej S. Januszewski, Tunde Peto, Laima Brazionis, Nanda Aryal, Rachel L. O'Connell, Liping Li, Paula Summanen, Russell Scott, Justin O'Day, Anthony C. Keech, Alicia J. Jenkins, FIELD Study Grp
DIABETES RESEARCH AND CLINICAL PRACTICE
(2023)
Article
Endocrinology & Metabolism
Andrzej S. Januszewski, Pawel Niedzwiecki, Nirupa Sachithanandan, Glenn M. Ward, Connie S. Karschimkus, David N. O'Neal, Dorota A. Zozulinska-Ziolkiewicz, Aleksandra A. Uruska, Alicia J. Jenkins
Summary: The aim of this study was to validate the accuracy of insulin sensitivity estimated by simple formulae derived from complex euglycemic hyperinsulinaemic clamp studies. Clamps were performed on 104 adults with Type 1 diabetes, and two formulae utilizing age, sex, HDL-C, HbA1c, pulse pressure, BMI, and waist-hip-ratio showed the highest correlation with measured glucose disposal rate and the best performance in detecting low insulin sensitivity.
DIABETES & METABOLIC SYNDROME-CLINICAL RESEARCH & REVIEWS
(2023)
Review
Ophthalmology
Laima Brazionis, Nicola Quinn, Sami Dabbah, Chris D. Ryan, Dennis M. Moller, Hilary Richardson, Anthony C. Keech, Andrzej S. Januszewski, Jakob Grauslund, Malin Lundberg Rasmussen, Tunde Peto, Alicia J. Jenkins
Summary: Advancements in retinal imaging technology have made it possible to quantitatively evaluate the retinal vasculature. Changes in retinal vessel caliber and geometry have been observed in various systemic diseases and neurodegenerative diseases. Several retinal vessel analysis softwares are available, some specific to certain diseases and others for a broader context. In research studies, semi-automated software has identified associations between retinal vessel caliber and geometry and the presence or risk of diseases such as diabetes mellitus, cardiovascular disease, and dementia. This article reviews and compares widely used semi-automated retinal vessel analysis softwares and their associations with ocular imaging findings in common systemic diseases. Original data comparing retinal caliber grading in individuals with Type 1 diabetes using two softwares is also provided, showing good agreement.
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
(2023)
Article
Medicine, Research & Experimental
Sahar I. Da'as, Ikhlak Ahmed, Waseem H. Hasan, Doua A. Abdelrahman, Elbay Aliyev, Sabah Nisar, Ajaz Ahmad Bhat, Mugdha V. Joglekar, Anandwardhan A. Hardikar, Khalid A. Fakhro, Ammira S. Al-Shabeeb Akil
Summary: This study identifies miR-223-3p as a potential biomarker for glycemic control and beta cell function in type 2 diabetes (T2D) patients, and reveals its association with diabetic retinopathy (DR) development. Functional validation in a zebrafish model demonstrates that overexpression of miR-223-3p leads to hyperglycemia and degenerated retinal vasculature. Targeting miR-223-3p may be a promising therapeutic strategy for controlling DR in T2D patients.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Biochemical Research Methods
Matthew B. O'Rourke, Andrzej S. Januszewski, David R. Sullivan, Imre Lengyel, Alan J. Stewart, Swati Arya, Ronald C. Ma, Sanjeev Galande, Anandwardhan A. Hardikar, Mugdha V. Joglekar, Anthony C. Keech, Alicia J. Jenkins, Mark P. Molloy
Summary: Robust and affordable plasma proteomic biomarker workflows are essential for large-scale clinical studies. This study evaluated various aspects of sample preparation for liquid chromatography-mass spectrometry (LC-MS) analysis of over 1500 samples from the FIELD trial. Optimized methods were applied in a pilot study, resulting in the identification of 172 proteins using LC-MS of undepleted plasma. The study also found that variations in blood collection tube type, delipidation methods, and freeze-thaw cycles had minimal impact on the results.
PROTEOMICS CLINICAL APPLICATIONS
(2023)
Meeting Abstract
Substance Abuse
F. Shihana, M. V. Joglekar, S. Tae--Hwi, P. Haber, A. A. Hardikar, D. Seth
ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH
(2022)
Meeting Abstract
Endocrinology & Metabolism
W. K. M. Wong, M. V. Joglekar, V. Saini, C. X. Dong, P. S. Kunte, B. L. Anderson, K. Z. Dajani, A. M. Simpson, P. E. MacDonald, P. A. Senior, K. K. Danielson, A. M. J. Shapiro, A. A. Hardikar
Meeting Abstract
Endocrinology & Metabolism
A. Grzelka-Wozniak, A. S. Januszewski, P. Niedzwiecki, A. J. Jenkins, D. Zozulinska-Ziolkiewicz, A. Uruska
Meeting Abstract
Endocrinology & Metabolism
A. S. Januszewski, L. M. Carroll, Y. Wen-Loh, M. L. H. Huang, D. N. O'Neal, A. J. Jenkins
