QuickStep-Cloning: a sequence-independent, ligation-free method for rapid construction of recombinant plasmids

Polishing the craft of genetic diversity creation in directed evolution

(2013) Kang Lan Tee et al.   BIOTECHNOLOGY ADVANCES

Exponential Megapriming PCR (EMP) Cloning—Seamless DNA Insertion into Any Target Plasmid without Sequence Constraints

(2013) Alexander Ulrich et al.   PLoS One

An asymmetric PCR-based, reliable and rapid single-tube native DNA engineering strategy

(2012) Yanzhen Bi et al.   BMC BIOTECHNOLOGY

Inverse Fusion PCR Cloning

(2012) Markus Spiliotis   PLoS One

Unidirectional cloning by cleaving heterogeneous sites with a single sandwiched zinc finger nuclease

(2011) Kazuki Shinomiya et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Phosphorothioate-based ligase-independent gene cloning (PLICing): An enzyme-free and sequence-independent cloning method

(2010) Milan Blanusa et al.   ANALYTICAL BIOCHEMISTRY

Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids

(2010) Anton Bryksin et al.   BIOTECHNIQUES

High-throughput cloning of human liver complete open reading frames using homologous recombination in Escherichia coli

(2009) Dewu Zhu et al.   ANALYTICAL BIOCHEMISTRY

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

A universal method for directional cloning of PCR products based on asymmetric PCR

(2008) Bao-Li Wang et al.   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY