Article
Biochemistry & Molecular Biology
Iris J. C. Dautzenberg, Martijn J. W. E. Rabelink, Rob C. Hoeben
Summary: This study evaluated the loss and stability of lentiviral vector particles during transduction of cells, showing that sensitivity to trypsin and half-life depend on the vector's envelope protein. Additionally, transferring culture medium to larger dishes can greatly reduce the quantity of vector particles in the culture.
Article
Biotechnology & Applied Microbiology
Jun Liu, Yingying Mao, Qingqing Li, Zhenzhen Qiu, Jingjing Li, Xiaoxin Li, Wenhan Liang, Mingyu Xu, Andrew Li, Xiangsheng Cai, Wangsheng Wu, Huangyao Chen, Renhe Yan, Jinlong Li, Weiwang Gu, Hongwei Li
Summary: Gene therapy has become an attractive field for studying various diseases, but progress in targeting kidney diseases has been limited due to low gene delivery efficiency in renal cells. In this study, a pseudotyped lentiviral vector called ZIKV-E was designed and produced, which showed high transduction efficiency in renal tubular epithelial cells. This vector could serve as a foundation for gene therapy for inherited renal diseases in humans.
HUMAN GENE THERAPY
(2022)
Review
Microbiology
Marcela Salazar-Garcia, Samyr Acosta-Contreras, Griselda Rodriguez-Martinez, Armando Cruz-Rangel, Alejandro Flores-Alanis, Genaro Patino-Lopez, Victor M. Luna-Pineda
Summary: This article introduces the method of using pseudotyped virus VSV-SARS-CoV-2 S for research and testing. Pseudovirus can be handled in a BSL-2 laboratory and is suitable for neutralizing antibody assays and viral fusion-focused treatment analysis.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Xiaodan Wu, Zhenyu Zhong, Yan Li, Yifan Wang, Yaping Tian, Xulong Liu, Xin Zhang, Weiyong Tao, Jianglin Wang, Yingying Du, Shengmin Zhang
Summary: The modification of immune cells through implanted biomaterials is a promising postoperative immunotherapy. An injectable hydrogel scaffold loaded with engineered exosome mimetics is developed, which recruits and programs endogenous macrophages into M1 binding with anti-CD47 antibody (M1-aCD47 macrophages) for postoperative cancer immunotherapy. The M1-aCD47 macrophages own dual-functions of tumor-homing and enhanced phagocytosis, and can inhibit tumor recurrence and metastasis by promoting phagocytosis of macrophages to cancer cells and T cell-mediated immune responses.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Min Gao, Tianhao Yang, Weiji Qin, Qian Wang, Mingyue Huang, Hui Peng, Meng Shao, Wanqing Yao, Xiaoqing Yi, Gengyun Sun, Xiaoyan He
Summary: A cell membrane-anchoring nano-photosensitizer (CMA-nPS) is developed to induce tumor-specific Ca2+ overload through disruption of endogenous Ca2+ homeostasis. The photosensitizer is anchored to the lung cancer cell membrane via functionalized cell membranes, resulting in membrane damage, mitochondrial dysfunction, and intracellular Ca2+ overload under light guidance. This approach shows synergistically enhanced antitumor efficacy in vitro and in vivo. It offers a new strategy for Ca2+-overload-based cancer therapy and anchoring photosensitizer on the cell membrane, with potential application in lung cancer treatment.
Article
Microbiology
Ariel Shepley-McTaggart, Jingjing Liang, Yang Ding, Marija A. Djurkovic, Valeriia Kriachun, Olena Shtanko, Oriol Sunyer, Ronald N. Harty
Summary: The study identifies filamin A and filamin B as regulators of Ebola and Marburg virus entry into cells. Filamin A promotes virus entry while filamin B inhibits it. These findings may lead to the development of broad spectrum therapeutic targets for preventing and treating Ebola and Marburg virus infections.
Article
Chemistry, Multidisciplinary
Li-Li Huang, Weiwei Wang, Zhongjie Wang, Han Zhang, Houli Liu, Guanghao Wu, Weidong Nie, Hai-Yan Xie
Summary: This study constructs an engineered oncolytic adenovirus (OA) that enhances the direct oncolysis and antitumor immune response. By decorating tumor cell membranes and utilizing their homing ability, the engineered OA can target specific tumors. The synergy of virus-mediated killing and immune effects leads to effective tumor inhibition.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Medicine, Research & Experimental
Takele Argaw, Michael P. Marino, Andrew Timmons, Lindsey Eldridge, Kazuyo Takeda, Pingjuan Li, Anna Kwilas, Wu Ou, Jakob Reiser
Summary: The use of Tupaia paramyxovirus (TPMV) envelope glycoproteins engineered to target human cell-surface receptors in lentiviral (LV) vectors allows for efficient transduction of cells expressing specific receptors. This novel pseudotyping strategy shows potential for developing targeted LV vectors for in vivo applications.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2021)
Article
Immunology
Hiva Azizi, Jason P. Knapp, Yue Li, Alice Berger, Marc-Alexandre Lafrance, Jannie Pedersen, Marc-Antoine de la Vega, Trina Racine, Chil-Yong Kang, Jamie F. S. Mann, Jimmy D. Dikeakos, Gary Kobinger, Eric J. Arts
Summary: The researchers used vesicular stomatitis virus (VSV) as a platform for a potential HIV-1 vaccine. However, they faced obstacles in selecting a highly immunogenic HIV-1 Envelope (Env) for maximum expression on recombinant VSV particles. By fusing the transmembrane domain (TM) and cytoplasmic tail (CT) of SIVMac239 with the HIV-1 Env, they created a novel chimera that expressed high levels on a VSV vector carrying the Ebola virus glycoprotein (GP). This new chimera, derived from a subtype A primary isolate, showed promising results in terms of antibody levels and neutralizing activity.
Article
Immunology
Teresa E. Sorvillo, Robert W. Cross, Dylan M. Johnson, Natalie S. Dobias, Karla A. Fenton, Chad E. Mire, Thomas W. Geisbert
Summary: The rVSV Delta G-JUNVGP vaccine showed excellent protective efficacy against JUNV virus in guinea pig models, with a 100% survival rate in vaccinated animals and robust antibody responses. This study represents the first demonstration of the vaccine's efficacy in providing full protection against lethal JUNV challenge with a single injection.
Article
Biotechnology & Applied Microbiology
Motomu Nakatake, Nozomi Kuwano, Emi Kaitsurumaru, Hajime Kurosaki, Takafumi Nakamura
Summary: The novel fusogenic oncolytic vaccinia virus (FUVAC) showed enhanced cytopathic effects and immunogenic cell death in cancer cells compared to its nonfusogenic parent virus. FUVAC not only inhibited tumor growth in both treated and untreated tumors in mice, but also improved the tumor immune microenvironment, leading to systemic antitumor immunity. Administering FUVAC before immune checkpoint inhibitor (ICI) treatment resulted in complete responses in both treated and untreated tumors, establishing a long-term tumor-specific immune memory.
Article
Pharmacology & Pharmacy
Jiayi Zhang, Annie Brown, Brendan Johnson, David Diebold, Kyle Asano, Gerard Marriott, Biao Lu
Summary: In this study, the effects of various membrane scaffolds on the physicochemical properties, molecular profiles, and cell uptake of extracellular vesicles (EVs) were investigated. The results showed that the incorporation of different molecular scaffolds altered the size, surface protein profiles, and cell uptake functions of EVs.
Article
Microbiology
Simone Bach, Jana-Christin Demper, Paul Klemm, Julia Schlereth, Marcus Lechner, Andreas Schoen, Lennart Kaemper, Friedemann Weber, Stephan Becker, Nadine Biedenkopf, Roland K. Hartmann
Summary: The study revealed the initiation of leaderRNA synthesis at nt 2 opposite to the genome, with termination predominantly occurring in the region of nt 60-80. The steady-state NP mRNA:leaderRNA ratio was determined after EBOV infection, showing higher levels in minigenome-transfected cells. Additionally, the synthesis of leaderRNA was found to be suppressed in the presence of VP30, and the termination of leaderRNA was not mediated by cryptic gene end (GE) signals in the 3'-leader promoter.
Article
Multidisciplinary Sciences
Mishar Kelishadi, Hosein Shahsavarani, Alijan Tabarraei, Mohammad Ali Shokrgozar, Ladan Teimoori-Toolabi, Kayhan Azadmanesh
Summary: Oncolytic viruses (OVs) are a promising cancer treatment modality that can selectively target and kill cancer cells. Researchers successfully engineered UL39 gene in HSV-1 to express EGFP-p53 using CRISPR/Cas9 editing approach, enhancing the oncolytic capabilities of the virus. HSV-1-P53 demonstrated cytolytic ability in various cell lines with different p53 expression rates.
Article
Chemistry, Physical
Sen Cui, Chunxiang Cui, Licong Kang, Shuangjin Liu
Summary: A casting strategy was developed to improve a Titanium-Aluminum alloy by effectively reinforcing a Ti3Al matrix alloy with in situ ceramic particles and inoculants, resulting in refined grain size and lamellar width, and enhanced mechanical properties. Additionally, SiC/Nb4C3 core-shell structures were observed in the interface, and their microstructure was studied in detail.
JOURNAL OF ALLOYS AND COMPOUNDS
(2021)