Journal
G3-GENES GENOMES GENETICS
Volume 4, Issue 6, Pages 1143-1145Publisher
GENETICS SOCIETY AMERICA
DOI: 10.1534/g3.114.010454
Keywords
Zea mays; insertion-deletion polymorphism; genetic mapping; molecular marker; positional cloning
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Funding
- National Science Foundation [IOS-1031416, IOS-1025976]
- National Institute of Food and Agriculture [2011-67003-30215, 2011-67013-30032]
- Division Of Integrative Organismal Sys
- Direct For Biological Sciences [1031416] Funding Source: National Science Foundation
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Positional cloning in maize (Zea mays) requires development of markers in the region of interest. We found that primers designed to amplify annotated insertion-deletion polymorphisms of seven base pairs or greater between B73 and Mo17 produce polymorphic markers at a 97% frequency with 49% of the products showing co-dominant fragment length polymorphisms. When the same polymorphisms are used to develop markers for B73 and W22 or Mo17 and W22 mapping populations, 22% and 31% of markers are co-dominant, respectively. There are 38,223 Indel polymorphisms that can be converted to markers providing high-density coverage throughout the maize genome. This strategy significantly increases the efficiency of marker development for fine-mapping in maize.
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