Article
Oncology
Han-Chao Feng, Christos Andreadis, Ji-Long Liu
Summary: The formation of cytoophidia in fission yeast is influenced by the histone chaperone Slm9, as knockout of the slm9 gene leads to fragmentation and changes in morphology of the cytoophidia. This study reveals a potential link between a chromosomal regulatory factor and cytoophidium biogenesis.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Plant Sciences
Daniel Hickl, David Scheuring, Torsten Moehlmann
Summary: CTPS2 plays an essential role in providing CTP supply during Arabidopsis embryo development, with its loss-of-function mutants being embryo lethal. The distinct expression pattern of CTPS2 throughout embryo development suggests a cell-type-specific regulation, and complementation with CTPS2 under its endogenous promoter rescues the observed phenotype.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Multidisciplinary Sciences
Jingyi Li, Tao Zhang, Tao Ren, Xiaoyu Liao, Yilong Hao, Je Sun Lim, Jong-Ho Lee, Mi Li, Jichun Shao, Rui Liu
Summary: This study identifies an oxygen-sensitive methylation of ULK1 with an important role in hypoxic stress adaptation by promoting autophagy induction.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Yufeng Wang, Yajing Lyu, Kangsheng Tu, Qiuran Xu, Yongkang Yang, Shaima Salman, Nguyet Le, Haiquan Lu, Chelsey Chen, Yayun Zhu, Ru Wang, Qingguang Liu, Gregg L. Semenza
Summary: It has been demonstrated that PADI4 expression is induced by hypoxia in a HIF-dependent manner, and is required for the transcription of HIF target genes. The recruitment of PADI4 by HIFs to target genes and local histone citrullination are essential for transcriptional responses to hypoxia.
Article
Multidisciplinary Sciences
Zsolt Karanyi, Agnes Mosolygo-L, Orsolya Fero, Adrienn Horvath, Beata Boros-Olah, Eva Nagy, Szabolcs Hetey, Imre Holb, Henrik Mihaly Szaker, Marton Miskei, Tibor Csorba, Lorant Szekvolgyi
Summary: Arabidopsis NODULIN HOMEOBOX (NDX) is a key regulator of heterochromatin that controls siRNA production and non-CG DNA methylation at pericentromeric regions. Inactivation of NDX leads to differential siRNA accumulation and DNA methylation. Hi-C analysis shows significant chromatin structural changes in the ndx mutant, with decreased intrachromosomal interactions at pericentromeres and increased interchromosomal contacts between KNOT-forming regions.
NATURE COMMUNICATIONS
(2022)
Article
Genetics & Heredity
Mathilde Dura, Aurelie Teissandier, Melanie Armand, Joan Barau, Clementine Lapoujade, Pierre Fouchet, Lorraine Bonneville, Mathieu Schulz, Michael Weber, Laura G. Baudrin, Sonia Lameiras, Deborah Bourc'his
Summary: DNA methylation is crucial for spermatogenesis, and the sterility of DNA methyltransferase mutant mice demonstrates its role. In this study, we uncover the division of labor in establishing the methylation landscape of male germ cells and its functions in spermatogenesis. DNMT3C prevents retrotransposons from interfering with meiosis, while DNMT3A broadly methylates the genome (except DNMT3C-dependent retrotransposons) and regulates spermatogonial stem cell (SSC) plasticity. Single-cell RNA sequencing and chromatin profiling reveal that Dnmt3A mutant SSCs can only self-renew and lose the ability to differentiate due to spurious enhancer activation. These findings highlight the importance of DNA methylation in male fertility: the epigenetic programming of SSC commitment to differentiation and lifelong spermatogenesis supply.
Article
Cell Biology
Yuanjiao Du, Weiping Chang, Lei Gao, Lin Deng, Wei-Ke Ji
Summary: In this study, Tex2, a lipid transfer protein found on the tubular endoplasmic reticulum, was discovered to be recruited to late endosomes/lysosomes by TMEM55, phosphatases that convert PI(4,5)P-2 to PI5P on LE/lys. The interaction between Tex2 and TMEM55 is regulated by endosome-resident type 2 PI4K activities. Tex2 knockout results in defects in lysosomal trafficking, digestive capacity, and lipid composition of LE/lys membranes. These findings highlight the importance of Tex2 in maintaining lysosomal functions through its presence at TMEM55-dependent ER-LE/lys MCSs.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Suchetana B. Dutta, Gerit Arne Linneweber, Maheva Andriatsilavo, Peter Robin Hiesinger, Bassem A. Hassan
Summary: The development of neuronal connectivity requires stabilization of dynamic axonal branches at synapse formation sites. In the study using Drosophila brain as a model system, it was found that epidermal growth factor receptor (EGFR) activity plays a crucial role in regulating circuit wiring during two distinct temporal intervals. EGFR is required for primary axonal branching early on, and then independently required later to prevent degradation of the synaptic active zone protein Bruchpilot (Brp). This temporally restricted molecular mechanism is essential for coupling axonal branching and synaptic stabilization.
Article
Biochemistry & Molecular Biology
Rachel Curnock, Katy Yalci, Johan Palmfeldt, Marja Jaattela, Bin Liu, Bernadette Carroll
Summary: The accumulation of senescent cells is a driver of aging, and one characteristic of senescent cells is an increase in lysosomal content. However, little is known about the causes and consequences of lysosomal biogenesis in senescence.
Review
Biochemistry & Molecular Biology
Stephen L. Bearne, Chen-Jun Guo, Ji-Long Liu
Summary: Cytidine-5'-triphosphate synthase (CTPS) is an enzyme involved in the biosynthesis of CTP. It requires an allosteric effector (GTP) to activate the glutamine hydrolysis process and also plays a role in the assembly and maintenance of the NH3 tunnel.
Article
Cell Biology
Matthew G. Lowe, Ming-Ren Yen, Fei-Man Hsu, Linzi Hosohama, Zhongxun Hu, Tsotne Chitiashvili, Timothy J. Hunt, Isaac Gorgy, Matthew Bernard, Sissy E. Wamaitha, Pao-Yang Chen, Amander T. Clark
Summary: The development of primordial germ cells (PGCs) is essential for reproduction. This study explores the role of embryonic ectoderm development (EED) and histone 3 lysine 27 trimethylation (H3K27me3) in sex-specific PGC differentiation. The findings suggest that EED plays a critical role in regulating the timing of sex-specific PGC differentiation and X chromosome dosage compensation in both ovaries and testes.
DEVELOPMENTAL CELL
(2022)
Article
Cell Biology
Mathieu Paquette, Leeanna El-Houjeiri, Linda C. Zirden, Pietri Puustinen, Paola Blanchette, Hyeonju Jeong, Kurt Dejgaard, Peter M. Siegel, Arnim Pause
Summary: It has been shown that AMPK regulates the transcriptional activity of TFEB and TFE3 through phosphorylation, with a significant impact during nutrient deprivation, FLCN depletion, and pharmacological manipulation of MTORC1 or AMPK. MTORC1 specifically controls the cytosolic retention of TFEB and TFE3, while AMPK is essential for their transcriptional activity. This dual and opposing regulation mechanism is reminiscent of the regulation of ULK1, another key regulator of autophagy.
Article
Biology
Claudia N. Spaan, Ruben J. de Boer, Wouter L. Smit, Jonathan H. M. van derMeer, Manon van Roest, Jacqueline L. M. Vermeulen, Pim J. Koelink, Marte A. J. Becker, Simei Go, Joana Silva, William J. Faller, Gijs R. van den Brink, Vanesa Muncan, Jarom Heijmans
Summary: This study found that mutations in APC are often followed by mutations in oncogene KRAS in the development of colorectal cancer, and the latter changes cellular metabolism and is associated with the Warburg phenomenon. The researchers hypothesize that targeting Grp78 in AK-mutant intestines interferes with the metabolic phenotype imposed by Kras mutations. They found that Grp78 plays a critical role in Kras-mutated adenomagenesis, possibly through its involvement in GLUT1 expression and localization, affecting glycolysis and the Warburg effect.
LIFE SCIENCE ALLIANCE
(2023)
Article
Biology
Yongchao Han, Lei Peng, Tao Wang, P. Robin Hiesinger
Summary: Neurotransmitters are crucial for synaptic transmission and are generated by de novo synthesis. In this study, a specific histidine transporter, TADR, was identified to play a critical role in visual transmission in Drosophila. These findings provide genetic evidence that TADR-dependent local de novo synthesis of histamine is necessary for synaptic transmission.
Article
Cell Biology
Claire E. Senner, Ziqi Dong, Malwina Prater, Miguel R. Branco, Erica D. Watson
Summary: One-carbon metabolism plays a crucial role in fetal development, but its molecular function is complex and unclear. This study reveals genome-wide epigenetic instability in Mtrr ( gt/gt ) placentas and identifies abnormal DNA methylation and expression of key genes, suggesting a role for histone modifications in epigenetic inheritance.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Chia-Liang Tsai, Ching-Yun Huang, Yi-Ching Lu, Li-Mei Pai, Daniel Horak, Yunn-Hwa Ma
Summary: In this study, it was found that cyclic strain reduces the internalization of magnetic nanoparticles (MNPs) by vascular smooth muscle cells (VSMCs), possibly due to decreased microvilli number and reduced vesicle size. Additionally, cyclic strain also decreases the uptake of other types of nanoparticles by cells. This finding can enhance the targeted delivery efficiency of nanomedicine.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Biology
Jiale Zhong, Chen-Jun Guo, Xian Zhou, Chia-Chun Chang, Boqi Yin, Tianyi Zhang, Huan-Huan Hu, Guang-Ming Lu, Ji-Long Liu
Summary: The bifunctional enzyme P5CS plays a vital role in proline and ornithine synthesis and its mutations can lead to human diseases and plant resistance impairment. We have discovered that P5CS forms cytoophidia in vivo and filaments in vitro, and have solved the structures of Drosophila full-length P5CS using cryo-electron microscopy. The filamentation of P5CS is crucial for the coordination between different domains and provides a structural basis for its catalytic function.
Article
Developmental Biology
Pei-Yu Wang, Archan Chakraborty, Hsin-Ju Ma, Jhen-Wei Wu, Anna C-C Jang, Wei-Cheng Lin, Hai-Wei Pi, Chau-Ting Yeh, Mei-Ling Cheng, Jau-Song Yu, Li-Mei Pai
Summary: This study highlights the regulatory role of cytidine triphosphate synthase (CTPsyn) in collective border cell migration through regulating the asymmetrical distribution of phosphatidylinositol (PI) 4,5-bisphosphate (PIP2).
Article
Virology
Wayne W. Yeh, Yin-Quan Chen, Wan-Shan Yang, Yung-Chih Hong, Sen Kao, Tze-Tze Liu, Ting-Wen Chen, Lung Chang, Pei-Ching Chang
Summary: This study investigates the role of KDM4A SUMOylation in primary effusion lymphoma (PEL) caused by KSHV infection. The findings suggest that SUMOylation of KDM4A plays a crucial role in PEL cell survival, movement, and angiogenesis. IL-10 is identified as a downstream target of KDM4A, and its involvement in PEL-induced angiogenesis is demonstrated. Targeting KDM4A may be a promising therapeutic option for treating PEL.
JOURNAL OF VIROLOGY
(2022)
Article
Cell Biology
Yi-Tung Chen, Chia-Hua Kan, Hsuan Liu, Yu-Hao Liu, Chih-Ching Wu, Yu-Ping Kuo, Ian Yi-Feng Chang, Kai-Ping Chang, Jau-Song Yu, Bertrand Chin-Ming Tan
Summary: Recent findings have highlighted the role of long noncoding RNAs (lncRNAs) in gene regulation, particularly in pathways associated with tumorigenesis. HOXA10-AS, a tumor-biased expressed lncRNA, plays a critical role in oral cancer growth and metastasis and is also associated with drug sensitivity.
CELL DEATH & DISEASE
(2022)
Article
Polymer Science
Hang Chen, Shurui Zhang, Yung-Chin Hsiao, Qun Wang, Jau-Song Yu, Wanming Li
Summary: The study developed a no-wash fluorescent aptasensor for sensitive detection of metastatic colorectal cancer (mCRC) cells. This aptasensor can detect target cells in one step and shows good specificity and sensitivity. Additionally, it can detect cancer cells in human whole blood with good reproducibility.
Article
Oncology
Chen-Yun Wang, Cing-Hong Wang, Ru-Tsun Mai, Ting-Wen Chen, Chia-Wei Li, Chi-Hong Chao
Summary: In this study, the researchers found that mutant p53 enhances fatty acid oxidation in basal-like breast cancer by upregulating CPT1C through dysregulating the miR-200c-ZEB2 axis. This contributes to metabolic preference changes, epithelial-mesenchymal transition, migration, invasion, and cancer stemness. Targeting mutant p53-CPT1C-driven metabolic reprogramming could be a promising therapeutic strategy.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tzu-Lung Lin, Cha-Chen Lu, Ting-Wen Chen, Chih-Wei Huang, Jang-Jih Lu, Wei-Fan Lai, Ting-Shu Wu, Chih-Ho Lai, Hsin-Chih Lai, Ya-Lei Chen
Summary: This study examined the effects of the probiotic strain P. goldsteinii MTS01 on autism spectrum disorder (ASD)-like behaviors in a mouse model. The results showed that P. goldsteinii MTS01 significantly reduced intestinal and systemic inflammation, improved gut health, and regulated multiple signaling pathways in the brain. These findings suggest that P. goldsteinii MTS01 has potential as a next-generation probiotic for ameliorating ASD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Infectious Diseases
Yung-Che Chen, Chang -Chun Hsiao, Chao-Chien Wu, Tung-Ying Chao, Sum-Yee Leung, Yu -Ping Chang, Chia-Cheng Tseng, Chiu-Ping Lee, Po-Yuan Hsu, Ting-Ya Wang, Po-Wen Wang, Ting-Wen Chen, Meng-Chih Lin
Summary: This study explores a new approach for treating active tuberculosis (TB) by screening and validating microRNAs with immune-regulatory functions. The research identified differentially expressed microRNAs associated with TB and revealed their roles in regulating autophagy, apoptosis, and macrophage phagocytosis. The findings provide evidence for microRNA-based host-directed immunotherapy and offer new therapeutic targets for treatment-refractory TB.
JOURNAL OF INFECTION
(2022)
Article
Food Science & Technology
Chih-Chuan Lin, Chia-Pang Shih, Chia-Cheng Wang, Chun-Hsiang Ouyang, Chien-Chun Liu, Jau-Song Yu, Chih-Hong Lo
Summary: The Taiwan bivalent freeze-dried hemorrhagic (FH) antivenom has been found to be effective in neutralizing venom and reducing limb swelling caused by snakebites from Protobothrops mucrosquamatus and Viridovipera stejnegeri in Taiwan.
Article
Oncology
Srinivas Dash, Chia-Chun Wu, Chih-Ching Wu, Sum-Fu Chiang, Yu-Ting Lu, Chien-Yuh Yeh, Jeng-Fu You, Lichieh Julie Chu, Ta-Sen Yeh, Jau-Song Yu
Summary: A study found that membrane proteins CD59 and TSPAN9 derived from colorectal cancer (CRC) cells are novel biomarkers for the early detection of CRC. Quantitative analysis of EV samples from 73 CRC patients and 80 healthy subjects identified CD59 and TSPAN9 as potential markers for distinguishing CRC patients from healthy individuals.
Article
Biochemistry & Molecular Biology
Hsiang-Pu Feng, Yu -Chin Liu, Chih-Liang Wang, Wei -Chao Liao, Jau-Song Yu, Chia-Jung Yu
Summary: KPNA2 is a nucleoplasmic protein responsible for nuclear import. Aberrant nuclear accumulation of KPNA2 has been observed in cancer tissues. AMPK is involved in the phosphorylation and acetylation of KPNA2, but its impact on the nucleocytoplasmic distribution of KPNA2 and its oncogenic role is unclear.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Cell Biology
Chung-Pu Wu, Cheng-Yu Hung, Ya-Ju Hsieh, Megumi Murakami, Yang-Hui Huang, Tsung-Yao Su, Tai-Ho Hung, Jau-Song Yu, Yu-Shan Wu, Suresh V. Ambudkar
Summary: The phosphoinositide-3-kinase (PI3K)/Akt signaling pathway plays a crucial role in tumor growth, and the drug HS-173, a PI3K inhibitor, has shown potential in halting cancer cell proliferation. However, this study found that the multidrug efflux transporters ABCB1 and ABCG2 can reduce the efficacy of HS-173 through drug-efflux processes. Inhibiting the drug-efflux function of ABCB1 and ABCG2 can restore the effectiveness of HS-173 in multidrug-resistant cancer cells.
Article
Medicine, General & Internal
Chien-Lun Chen, Yi-Ting Chen, Wen-Yu Liao, Yu-Sun Chang, Jau-Song Yu, Bao-Rong Juo
Summary: This study establishes a metabolomic platform for analyzing urine samples and identifies four metabolites related to prostate cancer. This research provides insights for future studies in this field.
