Article
Acoustics
Alexandra L. Wagner, Vera Danko, Anna Federle, Daniel Klett, David Simon, Rafael Heiss, Joerg Juengert, Michael Uder, Georg Schett, Markus F. Neurath, Joachim Woelfle, Maximilian J. Waldner, Regina Trollmann, Adrian P. Regensburger, Ferdinand Knieling
Summary: The study analyzed multi-wavelength optoacoustic imaging data in healthy adult volunteers, demonstrating good to excellent reproducibility for both intra- and inter-rater measurements, providing a framework for further clinical applications. However, challenges such as measurement time stability, exercise effects, and tissue variability still exist with this technology.
Article
Chemistry, Multidisciplinary
Lars-Philip Paulus, Adrian Buehler, Alexandra L. Wagner, Roman Raming, Joerg Juengert, David Simon, Koray Tascilar, Alexander Schnell, Ulrich Rother, Markus Eckstein, Werner Lang, Andre Hoerning, Georg Schett, Markus F. Neurath, Maximilian J. Waldner, Regina Trollmann, Joachim Woelfle, Sarah E. Bohndiek, Adrian P. Regensburger, Ferdinand Knieling
Summary: The use of multispectral optoacoustic tomography (MSOT) with indocyanine green (ICG) as a fluorescent dye allows real-time imaging and functional assessment of the gastrointestinal tract. This approach provides a non-ionizing and translatable method for clinical diagnosis.
Article
Acoustics
Adrian P. Regensburger, Alexandra L. Wagner, Vera Danko, Joerg Juengert, Anna Federle, Daniel Klett, Stephanie Schuessler, Adrian Buehler, Markus F. Neurath, Andreas Roos, Hanns Lochmueller, Joachim Woelfle, Regina Trollmann, Maximilian J. Waldner, Ferdinand Knieling
Summary: Proximal spinal muscular atrophy (SMA) is a rare genetic motor neuron disease with limited treatment monitoring biomarkers. A study demonstrated that handheld Multispectral Optoacoustic Tomography (MSOT) can visualize and quantify muscle wasting in pediatric SMA patients, showing significant differences in optoacoustic signal patterns compared to healthy volunteers. This non-invasive imaging approach could potentially provide a valuable tool for assessing disease burden in SMA patients.
Article
Multidisciplinary Sciences
Yinglong Wu, Lihe Sun, Xiaokai Chen, Jiawei Liu, Juan Ouyang, Xiaodong Zhang, Yi Guo, Yun Chen, Wei Yuan, Dongdong Wang, Ting He, Fang Zeng, Hongzhong Chen, Shuizhu Wu, Yanli Zhao
Summary: The authors address the challenges in using organic small-molecule contrast agents for multispectral optoacoustic imaging by constructing water-dispersible supramolecular optoacoustic assemblies based on cucurbit[8]uril. The constructed assemblies show enhanced optoacoustic performance due to increased absorption and decreased fluorescence. The formulated assemblies can effectively detect and diagnose various tumors and kidney injury in mouse models using multispectral optoacoustic imaging.
NATURE COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Adrian P. Regensburger, Emma Brown, Gerhard Kronke, Maximilian J. Waldner, Ferdinand Knieling
Summary: Optoacoustic or photoacoustic imaging technology allows non-invasive visualization of laser-illuminated tissue with high resolution and penetration depth, making it a versatile tool for biomedical research. When it comes to imaging inflammation, this technology offers advantages over current clinical methods in diagnosing and monitoring physiological and pathophysiological processes.
Article
Engineering, Biomedical
Nian Liu, Patrick O'Connor, Vipul Gujrati, Dimitris Gorpas, Sarah Glasl, Andreas Blutke, Axel Walch, Karin Kleigrewe, Michael Sattler, Oliver Plettenburg, Vasilis Ntziachristos
Summary: This study introduces the use of CR760 for nanoparticle formulation, optoacoustic imaging, and photothermal therapy. CR760 exhibits excellent optical properties, can be conveniently prepared in a single step, and can be covalently attached to cancer-targeting ligands to form nanoparticles with active tumor-targeting capability.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Optics
Paul Vetschera, Benno Koberstein-Schwarz, Tobias Schmitt-Manderbach, Christian Dietrich, Wibke Hellmich, Andrei Chekkoury, Panagiotis Symvoulidis, Josefine Reber, Gil Westmeyer, Hernan Lopez-Schier, Murad Omar, Vasilis Ntziachristos
Summary: Zebrafish, commonly used in developmental biology, have been recently studied in cancer research, tissue regeneration, metabolic disorders, functional genomics, and drug discovery. A novel multimodality system combining selective plane illumination microscopy (SPIM) and optoacoustic mesoscopy has been developed to observe zebrafish from larval stage to adulthood. This system enables visualization beyond the first days of development and allows for molecular imaging interrogation in adult fish. In addition, the optoacoustic modality reveals zebrafish features not visible with SPIM, providing a wider range of applications and time-scales for zebrafish studies.
LASER & PHOTONICS REVIEWS
(2023)
Review
Chemistry, Multidisciplinary
Yinglong Wu, Fang Zeng, Yanli Zhao, Shuizhu Wu
Summary: Optoacoustic imaging is a hybrid biomedical imaging technique that uses contrast agents to generate ultrasound waves in tissues, producing high-resolution images. Multispectral optoacoustic imaging can discriminate signals from different contrast agents and enable specific agent identification in tissues, as well as simultaneous molecular and physiological imaging. Contrast agents play essential roles in optoacoustic imaging, with various new agents being explored and applied in recent years.
CHEMICAL SOCIETY REVIEWS
(2021)
Article
Multidisciplinary Sciences
Tiago Granja, Sergio Faloni de Andrade, Luis Monteiro Rodrigues
Summary: Microcirculatory impairment is an important theme in vascular research. The development of live imaging systems, including optoacoustic tomography (OT), has provided new opportunities for real-time, quantifiable functional imaging. OT combines optical absorption and spatial resolution capacities with tissue depth, and shows great potential in functional imaging.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Computer Science, Interdisciplinary Applications
Justine Robin, Ali Ozbek, Michael Reiss, Xose Luis Dean-Ben, Daniel Razansky
Summary: Spherical matrix arrays, combined with clinically-approved microbubble injection, enable multimodal imaging for non-invasive mapping of vascular networks and oxygenation in deep tissues. In experiments, this method achieved high spatial resolution and signal to noise ratio, while allowing for flow velocity mapping.
IEEE TRANSACTIONS ON MEDICAL IMAGING
(2022)
Review
Genetics & Heredity
Nian Liu, Xiao Chen, Melanie A. Kimm, Matthias Stechele, Xueli Chen, Zhimin Zhang, Moritz Wildgruber, Xiaopeng Ma
Summary: Optical imaging technology has great potential in detecting inflammation, providing accurate molecular information and offering new insights into the diagnosis and therapeutic evaluation of inflammatory diseases. By using endogenous and exogenous contrast agents, optical imaging technology can accurately visualize sites of inflammation, enabling early disease detection and specific disease characterization.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2021)
Article
Nanoscience & Nanotechnology
Margarita R. Chetyrkina, Julijana Cvjetinovic, Fedor S. Fedorov, Stanislav Perevoschikov, Ekaterina S. Prikhozhdenko, Bjorn F. Mikladal, Yuri G. Gladush, Albert G. Nasibulin, Dmitry A. Gorin
Summary: Optoacoustic imaging is a noninvasive and fast-growing technique used in both research and clinical practice. In this study, the researchers propose using fibers based on single-walled carbon nanotubes as a stable and reliable material for designing a calibration grid. The study demonstrates the feasibility of using these fibers for calibrating and verifying the spatial resolution and sensitivity of optoacoustic imaging systems.
Article
Biotechnology & Applied Microbiology
Yanting Wen, Dan Wu, Jing Zhang, Shixie Jiang, Chunyan Xiong, Dan Guo, Zihui Chi, Yi Chen, Lun Li, Ying Yang, Ting Liu, Huabei Jiang
Summary: This study evaluated the feasibility of using photoacoustic tomography (PAT) and multispectral optoacoustic tomography (MSOT) for detecting histology in a rabbit tracheal stenosis model. The results showed that PAT/MSOT can be used to evaluate tracheal structure and function in vivo.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemical Research Methods
Aniwat Juhong, Bo Li, Yifan Liu, Cheng-You Yao, Chia-Wei Yang, Dalen W. Agnew, Yu Leo Lei, Gary D. Luker, Harvey Bumpers, Xuefei Huang, Wibool Piyawattanametha, Zhen Qiu
Summary: Multispectral optoacoustic tomography (MSOT) is a useful technique for detailed analysis of biological samples, but it's time-consuming. This study proposes a deep learning model based on hybrid recurrent and convolutional neural networks to generate sequential cross-sectional images for MSOT. By using a larger step size for input images, the proposed model can generate additional high-resolution images, reducing acquisition time by approximately 71%.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Biochemical Research Methods
Alejandra Hernandez Pichardo, James Littlewood, Arthur Taylor, Bettina Wilm, Raphael Levy, Patricia Murray
Summary: Tracking the fate of therapeutic cell types is crucial for evaluating their safety and effectiveness. Bioluminescence imaging (BLI) is effective but lacks spatial resolution, so a bimodal imaging approach that combines BLI with high-resolution techniques is necessary. In this study, the effectiveness of combining multispectral optoacoustic tomography (MSOT) or micro-computed tomography (micro-CT) with BLI was compared for tracking gold nanorod-labeled human mesenchymal stromal cells (MSCs). MSOT was found to be more sensitive than micro-CT in tracking the labeled cells in vivo, and when combined with BLI, it effectively tracked the fate of MSCs in mice depending on the route of administration.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Computer Science, Interdisciplinary Applications
Daniele Ancora, Athanasios Zacharopoulos, Jorge Ripoll, Giannis Zacharakis
IEEE TRANSACTIONS ON MEDICAL IMAGING
(2017)
Article
Immunology
Markus Ackerknecht, Kathrin Gollmer, Philipp Germann, Xenia Ficht, Jun Abe, Yoshinori Fukui, Jim Swoger, Jorge Ripoll, James Sharpe, Jens V. Stein
JOURNAL OF IMMUNOLOGY
(2017)
Article
Biochemical Research Methods
Matthias Rieckher, Stylianos E. Psycharakis, Daniele Ancora, Evangelos Liapis, Athanasios Zacharopoulos, Jorge Ripoll, Nektarios Tavernarakis, Giannis Zacharakis
BIOTECHNOLOGY JOURNAL
(2018)
Article
Biochemical Research Methods
Daniele Ancora, Diego Di Battista, Georgia Giasafaki, Stylianos E. Psycharakis, Evangelos Liapis, Jorge Ripoll, Giannis Zacharakis
Article
Cell Biology
Claudio Cantu, Anastasia Felker, Dario Zimmerli, Karin D. Prummel, Elena M. Cabello, Elena Chiavacci, Kevin M. Mendez-Acevedo, Lucia Kirchgeorg, Sibylle Burger, Jorge Ripoll, Tomas Valenta, George Hausmann, Nathalie Vilain, Michel Aguet, Alexa Burger, Daniela Panakova, Konrad Basler, Christian Mosimann
GENES & DEVELOPMENT
(2018)
Review
Anatomy & Morphology
Ignacio Albert-Smet, Asier Marcos-Vidal, Juan Jose Vaquero, Manuel Desco, Arrate Munoz-Barrutia, Jorge Ripoll
FRONTIERS IN NEUROANATOMY
(2019)
Article
Engineering, Biomedical
Wuwei Ren, Helene Isler, Martin Wolf, Jorge Ripoll, Markus Rudin
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING
(2020)
Article
Optics
Wuwei Ren, Jingjing Jiang, Aldo Di Costanzo Mata, Alexander Kalyanov, Jorge Ripoll, Scott Lindner, Edoardo Charbon, Chao Zhang, Markus Rudin, Martin Wolf
Review
Multidisciplinary Sciences
Maria Victoria Gomez-Gaviro, Daniel Sanderson, Jorge Ripoll, Manuel Desco
Article
Biochemical Research Methods
R. Fernandez, A. Marcos-Vidal, S. Gallego, A. Belendez, M. Desco, J. Ripoll
BIOMEDICAL OPTICS EXPRESS
(2020)
Article
Biochemical Research Methods
Wuwei Ren, Linlin Li, Jianru Zhang, Markus Vaas, Jan Klohs, Jorge Ripoll, Martin Wolf, Ruiqing Ni, Markus Rudin
Summary: Abnormal accumulation of amyloid-beta peptide (Aβ) in the brain is a major characteristic of Alzheimer's disease. This study demonstrates the feasibility of visualizing Aβ deposition in 3D using a multimodal FMT-MRI strategy, providing potential for detailed characterization of disease status and monitoring of treatment efficacy.
BIOMEDICAL OPTICS EXPRESS
(2022)
Proceedings Paper
Optics
Stylianos E. Psycharakis, Evangelos Liapis, Athanasios Zacharopoulos, Mariam-Eleni Oraiopoulou, Chrysoula Aivalioti, Vangelis Sakkalis, Joseph Papamatheakis, Jorge Ripoll, Giannis Zacharakis
ADVANCES IN MICROSCOPIC IMAGING II
(2019)
Proceedings Paper
Engineering, Biomedical
Mavi Nunn Polatoglu, Yan Liu, Ruiqing Ni, Jorge Ripoll, Markus Rudin, Martin Wolf, Wuwei Ren
DIFFUSE OPTICAL SPECTROSCOPY AND IMAGING VII
(2019)
Meeting Abstract
Biochemistry & Molecular Biology
Jun Abe, Philipp Germann, Jorge Ripoll, James Sharpe, Jens V. Stein
Article
Developmental Biology
Maria Victoria Gomez-Gaviro, Evan Balaban, Diana Bocancea, Maria Teresa Lorrio, Maria Pompeiano, Manuel Desco, Jorge Ripoll, Juan Jose Vaquero