Journal
ACS MACRO LETTERS
Volume 7, Issue 10, Pages 1220-1225Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsmacrolett.8b00565
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- Korean Institute for Basic Science [IBS-R020-D1]
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Bulk-level measurements of dynamics have suggested that phase-separated, protein-nucleic acid rich droplets can be viewed as simple liquids. In this report, we show that histone proteins spontaneously phase separate into liquid-like droplets in the presence of DNA. Using super-resolution fluorescence microscopy, we find that molecular transport in these droplets is non-Fickian (subdiffusive) at nanoscopic length scales. This observation cannot be explained by charge-charge interactions. Instead, our results strongly suggest that cation-pi interactions drive the non-Fickian behavior. Given the ubiquity of cationic and aromatic moieties in protein-nucleic acid rich, liquid-like phases observed in cells, we anticipate that non-Fickian diffusion is a general transport mechanism in such phases.
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