Article
Clinical Neurology
Keun Woo Park, Hyunwoo Ju, Il-Doo Kim, John W. Cave, Yang Guo, Wei Wang, Zhuhao Wu, Sunghee Cho
Summary: The study found that monocyte-derived macrophages (MDMs) play a role in the progressive stroke-induced injury and repair processes. Manipulating monocyte entry at different stroke stages may be an effective immune-based strategy to limit injury propagation in chronic stroke.
Article
Biochemistry & Molecular Biology
Svitlana Skoroplyas, Ondrej Vasicek, Michaela Chorvatova, Petra Dad'ova, Katerina Lehka, Lukas Kubala
Summary: Pseurotin D has the potential to inhibit DTH reaction through inhibiting the activation and differentiation of both T cells and B cells.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Review
Oncology
Ines Roger, Paula Montero, Martin Perez-Leal, Javier Milara, Julio Cortijo
Summary: The clinical practice faces the problem of delayed-type hypersensitivity (DTH) caused by various drugs, especially in antineoplastic treatments. DTH reactions can range from mild self-limiting maculopapular eruptions to severe Stevens-Johnson Syndrome, resulting in worsened patient quality of life. Apart from impacting patients, these reactions also lead to economic losses due to drug withdrawal and high hospitalization costs. However, there is currently no standard in vitro or in vivo method to evaluate the sensitizing potential of drugs in preclinical studies. This review aims to comprehensively evaluate in vitro and in vivo methods for detecting DTH and testing antineoplastic hypersensitivity reactions caused by different antineoplastic families.
Article
Chemistry, Medicinal
Ines Roger, Paula Montero, Antonio Garcia, Javier Milara, Pilar Ribera, Jose Alejandro Perez-Fidalgo, Julio Cortijo
Summary: Delayed-type hypersensitivity (DTH) is a common reaction caused by various drugs, especially antineoplastic drugs, which may affect patients' quality of life. However, there is currently a lack of standardized methods for evaluating the sensitizing potential of drugs in the preclinical phase. This study aimed to adapt in vitro techniques to predict the sensitizing potential of antineoplastic agents and discovered that inhibition of the VEGFR1 pathway may be a potential trigger of DTH.
Article
Chemistry, Analytical
Mousumi Baruah, Haw-Young Kwon, Heewon Cho, Young-Tae Chang, Animesh Samanta
Summary: The differentiation of macrophage phenotypes is crucial for accurate diagnosis and treatment of inflammatory diseases. A fluorescent probe called PMS-T has been developed to distinguish M1 from M0 and M2 macrophages by detecting the levels of hypochlorite (OCl-). This probe has the potential to serve as a chemical tool for monitoring inflammatory diseases, such as rheumatoid arthritis, and overcome the limitations of immunohistochemistry.
ANALYTICAL CHEMISTRY
(2023)
Article
Immunology
Yuehan Gao, He Li, Zhaohuai Li, Lihui Xie, Xiuxing Liu, Zhaohao Huang, Binyao Chen, Xianchai Lin, Xianggui Wang, Yingfeng Zheng, Wenru Su
Summary: The study identified different subtypes within human moDCs and blood DCs, revealing their transcriptomic differences and differentiation trajectories, which will enable more accurate functional and developmental analyses of human cDC2s and moDCs.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Lisa Willemsen, Hung-Jen Chen, Cindy P. A. A. van Roomen, Guillermo R. Griffith, Ricky Siebeler, Annette E. Neele, Jeffrey Kroon, Marten A. Hoeksema, Menno P. J. de Winther
Summary: This study discovered that lipid accumulation in macrophages suppresses the expression of interferon-stimulated genes (ISGs) and secretion of IFN-beta, which can be rescued by exogenous IFN-beta supplementation. Activation of the cholesterol-sensing nuclear liver X receptor (LXR) recapitulated the cholesterol-initiated interferon suppression. Similar interferon suppression was observed in monocytes of familial hypercholesterolemia patients. These findings provide new insights into controlling inflammation in hyperlipidemic conditions and support future therapeutic approaches targeting macrophage reprogramming to reduce atherosclerotic plaque progression and improve stability.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Subhadeep Das, Abhilasha Batra, Subhankar Kundu, Rati Sharma, Abhijit Patra
Summary: By employing time-resolved imaging and DC-Lyso fluorescent probe, we investigated the dynamic polarity of lysosomes in live cells and nematodes. DC-Lyso rapidly labels lysosomes and exhibits polarity-sensitive long lifetime. The changes in fluorescence lifetime of DC-Lyso suggest an increase in local polarity during lysosomal dynamics and interorganelle interactions.
Article
Medicine, Research & Experimental
Roman Mehling, Johannes Schwenck, Christina Lemberg, Christoph Trautwein, Laimdota Zizmare, Daniela Kramer, Anne Mueller, Birgit Fehrenbacher, Irene Gonzalez-Menendez, Leticia Quintanilla-Martinez, Katrin Schroeder, Ralph P. Brandes, Martin Schaller, Wolfram Ruf, Martin Eichner, Kamran Ghoreschi, Martin Roecken, Bernd J. Pichler, Manfred Kneilling
Summary: ROS/RNS play a dominant role in acute and chronic delayed-type hypersensitivity reactions, with MPO and NOX2 identified as the main sources. However, depletion of only one primary source has marginal and conflicting effects on the outcomes of acute and chronic diseases, demonstrating that ROS/RNS are not a singular entity with different properties at various disease stages.
Article
Chemistry, Medicinal
Katarzyna Nazimek, Eugenio Bustos-Moran, Noelia Blas-Rus, Bernadeta Nowak, Justyna Toton-Zuranska, Michal T. Seweryn, Pawel Wolkow, Olga Woznicka, Rafal Szatanek, Maciej Siedlar, Philip W. Askenase, Francisco Sanchez-Madrid, Krzysztof Bryniarski
Summary: This study demonstrates that suppressor T cell-derived EVs carrying miRNA-150 can antigen-specifically downregulate mouse delayed-type hypersensitivity. Pre-incubation with antigen-specific antibodies enhances the inhibitory effect of macrophage-released EVs on effector T cells. The specific binding of EVs to MHC class II-expressing EVs promotes their aggregation and significantly increases their suppressive activity in vivo, potentially offering therapeutic benefits.
Article
Immunology
Alycia P. Fratzke, Anthony E. Gregory, Erin J. van Schaik, James E. Samuel
Summary: Q-VAX (R), a vaccine for Q fever, provides long-term protection but can cause reactions in sensitized individuals, limiting its use outside Australia. Studying different mouse strains revealed that the reactions to C. burnetii whole cell vaccine are consistent with a Th1 delayed-type hypersensitivity response.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Ju Hui Yun, Kyung Hyung Lee, Jun Yeob Lee
Summary: The benzoylphenyltriazine acceptor is explored as a strong acceptor for TADF emitters due to its high external quantum efficiency. By adding a weak electron-withdrawing benzoyl unit, the acceptor's electron-accepting strength is improved, leading to enhanced light emission performance of TADF emitters. Comparing with a phenyltriazine-based emitter, the benzoylphenyltriazine acceptor shows superior delayed-fluorescence characteristics with a higher quantum efficiency.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2021)
Review
Allergy
Feng Xu, Hongting Zhang, Hao Xiao, Juan Meng
Summary: This case report highlights a delayed-type IV allergic reaction to furazolidone in a patient receiving treatment for H.pylori infection. Clinicians should be aware of the potential for hypersensitivity to furazolidone.
CONTACT DERMATITIS
(2023)
Article
Engineering, Electrical & Electronic
Sung Yong Byeon, Seung Chan Kim, Jun Yeob Lee
Summary: The new blue fluorophores 1TCzTrz and 13TCzTrz derived from a triscarbazole donor and a diphenyltriazine acceptor were studied for their structure-property relationship, revealing that 1TCzTrz showed higher luminescence efficiency and external quantum efficiency compared to 13TCzTrz, although the latter was advantageous in increasing horizontal emitting dipole orientation.
ACS APPLIED ELECTRONIC MATERIALS
(2022)
Article
Allergy
Heena Mehta, Carolyn Jack, Catherine Maari, Etienne Saint-Cyr Proulx, Robert Bissonnette, Francisco Ramirez-Valle, Marika Sarfati
Summary: The potential benefit of inducing delayed-type hypersensitivity (DTH) reaction in healthy volunteers as experimental models to study skin inflammatory disorders was reported. This study investigated the immunophenotype of skin T cells and their modulation by oral drugs in a local DTH reaction.
Review
Biology
Zhao Feng, Anan Li, Hui Gong, Qingming Luo
Summary: This article provides a brief history of the development of stereotaxic brain atlases and clarifies key conceptual elements and technical indices. It discusses advanced technologies and future trends in image acquisition, coordinate construction, image processing, anatomical structure recognition, and publishing. The use of single-cell resolution and micron-level location precision is expected to become a major trend in the development of neuroscience through stereotaxic brain atlases.
SCIENCE CHINA-LIFE SCIENCES
(2022)
Article
Optics
Peng Wu, Dejie Zhang, Jing Yuan, Shaoqun Zeng, Hui Gong, Qingming Luo, Xiaoquan Yang
Summary: This paper presents a method combining deep learning with Fresnel incoherent correlation holography to achieve significant large depth of field (DoF) fluorescence microscopy. By using the Auto-ASP method and generative adversarial network, high-quality images with a DoF larger than traditional wide-field microscopy are obtained. The method has a simple structure and can be easily integrated with existing fluorescence microscopic imaging technologies.
Article
Optics
Tianpeng Luo, Jing Yuan, Jin Chang, Yanfeng Dai, Hui Gong, Qingming Luo, Xiaoquan Yang
Summary: In traditional fluorescence microscopy, achieving a large uniform imaging field with high resolution is difficult. In this study, we developed a confocal fluorescence microscope that combines a microlens array with a spatial light modulator to address this issue. Our system uses a multi-spot array generated by a spatial light modulator and a microlens array to form an optical probe array. We introduced a multi-spot adaptive pixel-reassignment method for image scanning microscopy (MAPR-ISM) to improve spatial resolution. Additionally, we employed an optimized double weighted Gerchberg-Saxton algorithm (ODWGS) with signal feedback from the camera to generate a uniform image. Our prototype system provides a lateral resolution of approximately 0.82 μm with about 1.6 times resolution enhancement after ISM processing, and exhibits a nonuniformity of 3% across the entire imaging field. Experimental results with fluorescent beads, mouse brain slices, and melanoma slices were presented to validate the applicability and effectiveness of our system.
Article
Optics
K. A. I. X. I. A. N. Liu, Y. U. X. U. A. N. Jiang, W. E. N. S. O. N. G. LI, H. A. I. T. A. O. Chen, Q. I. N. G. M. I. N. G. Luo, Y. O. N. G. Deng
Summary: In this study, a spatially adaptive split Bregman network (SSB-Net) was proposed to overcome the spatial nonuniformity of measurement sensitivity and ill-posed reconstruction in mesoscopic fluorescence molecular tomography (MFMT) in reflective geometry. The SSB-Net was derived from the split Bregman algorithm and utilized residual blocks and 3D convolution neural networks (3D-CNNs) to learn spatially nonuniform error compensation, spatially dependent proximal operator, and sparsity transformation. Simulations and experiments showed that the SSB-Net enabled high-fidelity MFMT reconstruction within a depth of a few millimeters, paving the way for practical reflection-mode diffuse optical imaging technique.
Article
Optics
Xiaojun Zhao, Guangcai Liu, Rui Jin, Hui Gong, Qingming Luo, Xiaoquan Yang
Summary: Fluorescence microscopy is often affected by system and sample-induced aberration, which can be solved by image deconvolution. We proposed a novel Richardson-Lucy model-driven deconvolution framework that improves reconstruction performance and speed. Within this framework, we designed two kinds of neural networks that are partially interpretable compared to previous deep learning methods. We introduced Richardson-Lucy into deep feature space, which has better generalizability than convolutional neural networks (CNN). We further accelerated the process with an unmatched backprojector, achieving a five times faster reconstruction speed than classic RL. Our deconvolution approaches outperform both CNN and traditional methods in terms of image quality for blurred images caused by out-of-focus or imaging system aberration.
Article
Medicine, Research & Experimental
Songlin Huang, Borui Li, Zheng Liu, Mengli Xu, Dong Lin, Jiahong Hu, Dongjian Cao, Qi Pan, Jing Zhang, Jing Yuan, Qingming Luo, Zhihong Zhang
Summary: This study proposed a method for selectively labeling hepatic lymphatic vessels (LVs) using sequential injection of antibodies (STSI-Ab) in vivo. The molecular information and function of hepatic lymphatic endothelial cells (LyECs) were further investigated. This research is valuable for understanding the structure and function of hepatic lymphatics.
Article
Chemistry, Multidisciplinary
Zheng Liu, Zhan Fan, Jinxin Liu, Jialu Wang, Mengli Xu, Xinlin Li, Yilun Xu, Yafang Lu, Chenlu Han, Zhihong Zhang
Summary: An immunomodulatory approach based on lipid nanoparticles carrying alpha-helical configurational melittin is developed to treat allergic contact dermatitis and atopic dermatitis by suppressing T cell-mediated inflammatory reactions.
Article
Optics
Wei Qiao, Yafeng Li, Kefu Ning, Qingming Luo, Hui Gong, Jing Yuan
Summary: Line confocal (LC) microscopy is a fast 3D imaging technique with limited resolution and optical sectioning. To overcome this, the differential synthetic illumination (DSI) method based on multi-line detection is proposed to enhance resolution and sectioning capability. DSI-LC improves resolution by 1.28x and 1.26x in the X and Z axes, respectively, and enhances optical sectioning by 2.6x compared to LC. It also demonstrates spatially resolved power and contrast in imaging various samples. DSI-LC provides a promising approach for 3D large-scale and functional imaging with improved resolution and robustness.
Article
Optics
Yuxuan Jiang, Kaixian Liu, Wensong Li, Qingming Luo, Yong Deng
Summary: We propose a deep background-mismodeling-learned reconstruction framework for high-accuracy fluorescence diffuse optical tomography (FDOT). A learnable regularizer incorporating background mismodeling is formulated as mathematical constraints and is automatically learned using a physics-informed deep network. Experimental results demonstrate the significant improvement in FDOT accuracy by implicitly learning the background mismodeling, validating the effectiveness of the proposed framework. It can also be applied as a general method to improve image modalities based on linear inverse problems with unknown background modeling errors.
Article
Optics
Mengli Xu, Zheng Liu, Xinlin Li, Xinru Wang, Xuenan Yuan, Chenlu Han, Zhihong Zhang
Summary: The liver is the largest internal organ of the human body, responsible for blood circulation, material metabolism, and immune response. Optical imaging is an effective tool for studying the liver's fine vascular structure and immunocyte distribution. This article provides an overview of the liver's structure, three-dimensional imaging, and immune function of various cell components, with a focus on 3D imaging methods. It also summarizes and prospects the development of 3D imaging of liver vessels and immune cells.
JOURNAL OF INNOVATIVE OPTICAL HEALTH SCIENCES
(2023)
Article
Chemistry, Analytical
Shuang Yang, Yutong Han, Yafeng Li, Lei Zhang, Guoquan Yan, Jing Yuan, Qingming Luo, Huali Shen, Xiaohui Liu
Summary: Recent developments in phosphoproteomics have enabled the identification and quantification of over 10,000 phosphosites, but current analyses are limited in sample size, reproducibility, and robustness. To address these challenges, the miniPhos method was introduced, which uses a minimal amount of sample to obtain sufficient information for deciphering biological significance. This method allows for rapid sample pretreatment and high efficiency phosphopeptide collection, resulting in the quantification of thousands of phosphosites and providing insights into cellular regulation in the mouse brain.
ANALYTICAL CHEMISTRY
(2023)
Article
Computer Science, Interdisciplinary Applications
Tianfang Zhu, Gang Yao, Dongli Hu, Chuangchuang Xie, Pengcheng Li, Xiaoquan Yang, Hui Gong, Qingming Luo, Anan Li
Summary: This study proposes MorphoGNN, a single neuron morphological embedding based on a graph neural network. By considering the point-level structure information of reconstructed nerve fibers, MorphoGNN captures the lower-dimensional representation of a single neuron and demonstrates cutting-edge performance in tasks such as neuron classification, retrieval, reconstruction quality classification, and neuron clustering.
IEEE TRANSACTIONS ON MEDICAL IMAGING
(2023)
Article
Biochemistry & Molecular Biology
Juan Chen, Haihua Ma, Zhiyu Deng, Qingming Luo, Hui Gong, Ben Long, Xiangning Li
Summary: Organoids have the potential to replicate human phenotypes and functions, making them valuable for developmental research, disease modeling, and drug screening. A comprehensive strategy involving array embedding, staining, and imaging of organoids in both agarose sections and in 3D was developed to analyze biomarker distribution and study phenotypic changes. The strategy allows for easy comparison between different groups and provides valuable insights into disease models and drug screening.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Yunfei Zhang, Guangcai Liu, Xiangning Li, Hui Gong, Qingming Luo, Xiaoquan Yang
Summary: Optical microscopy is a powerful tool for studying the structure and function of organisms, but imaging large volume samples in three dimensions is time-consuming and challenging. In this study, we developed an online clearing and staining method that allows for efficient imaging of large volume samples at cellular resolution. By optimizing the staining and imaging depth, we were able to reduce sectioning and scanning time and increase the operational efficiency of the system by more than two-fold. Using this method, we successfully obtained high-resolution images of Aβ plaques in the whole mouse brain and complete cytoarchitecture images of an adult porcine hemisphere in approximately 49 hours.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Cell Biology
Yutong Han, Zhan Zhang, Yafeng Li, Guoqing Fan, Mengfei Liang, Zhijie Liu, Shuo Nie, Kefu Ning, Qingming Luo, Jing Yuan
Summary: Automated evaluation of all glomeruli throughout the whole kidney is crucial for studying kidney function and understanding kidney disease mechanisms. This study proposes a deep learning-based segmentation method called FastCellpose to efficiently segment all glomeruli in whole mouse kidneys. The method shows superior performance compared to other cellular segmentation methods and significantly improves processing speed. By using this high-performance framework, the researchers were able to quantitatively analyze the developmental changes of mouse glomeruli, providing new insights for kidney development and function research.