Journal
THERANOSTICS
Volume 3, Issue 6, Pages 395-408Publisher
IVYSPRING INT PUBL
DOI: 10.7150/thno.5935
Keywords
Xeno-nucleic acid; Nucleic acid; Molecular beacon; Diagnostics
Categories
Funding
- National Basic Research 973 Program [2013CB733700]
- Fundamental Research Funds for the Central Universities [WJ1213007]
- talent program of ECUST [YJ0142149]
- National Natural Science Foundation of China [21341001]
- Program of Shanghai Pujiang [K100-2-1275]
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Molecular beacons (MBs) of DNA and RNA have aroused increasing interest because they allow a continuous readout, excellent spatial and temporal resolution to observe in real time. This kind of dual-labeled oligonucleotide probes can differentiate between bound and unbound DNA/RNA in homogenous hybridization with a high signal-to-background ratio in living cells. This review briefly summarizes the different unnatural sugar backbones of oligonucleotides combined with fluorophores that have been employed to sense DNA/RNA. With different probes, we epitomize the fundamental understanding of driving forces and these recognition processes. Moreover, we will introduce a few novel and attractive emerging applications and discuss their advantages and disadvantages. We also highlight several perspective probes in the application of cancer therapeutics.
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