Article
Cell Biology
Serena Barachini, Marina Montali, Francesca M. Panvini, Vittoria Carnicelli, Gian Luca Gatti, Nicola Piolanti, Enrico Bonicoli, Michelangelo Scaglione, Gabriele Buda, Paolo D. Parchi
Summary: Mesangiogenic progenitor cells (MPCs) isolated from human bone marrow mononuclear cells have the ability to differentiate into mesenchymal stromal cells while retaining endothelial differentiative potential. Their power to couple mesengenesis and angiogenesis highlights their tissue regenerative potential, particularly in musculoskeletal tissues.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Domenico Aprile, Nicola Alessio, Ibrahim H. Demirsoy, Tiziana Squillaro, Gianfranco Peluso, Giovanni Di Bernardo, Umberto Galderisi
Summary: The study demonstrated that multi-lineage stem cells can be isolated from a mouse model, offering a valid alternative for disease research and cell therapy, and showing potential applications for these cells.
Article
Cell & Tissue Engineering
Sara Bucar, Andre Dargen de Matos Branco, Marcia F. Mata, Joao Coutinho Milhano, Iris Caramalho, Joaquim M. S. Cabral, Ana Fernandes-Platzgummer, Claudia L. da Silva
Summary: The study compared the capacity of MSC from different sources to expand UCB CD34(+) cells ex vivo, with AT-derived MSC showing superior expansion potential and maintenance of primitive cells. UCM-derived MSC demonstrated inferior hematopoietic supportive capacity compared to MSC from adult tissues. Further research is needed to determine the impact of HPL on the hematopoietic supportive capacity of MSC.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Agriculture, Dairy & Animal Science
Ejaz R. Dar, Mudasir B. Gugjoo, Moien Javaid, Shahid Hussain, Mujeeb R. Fazili, Kuldeep Dhama, Taha Alqahtani, Ali M. Alqahtani, Riaz A. Shah, Talha Bin Emran
Summary: The study evaluates the influence of ambient temperature, donor physiological status, and tissue source on sheep mesenchymal stem cells. Results show that adipose tissue-derived MSCs from full term pregnant female sheep exhibit higher potential for proliferation and differentiation compared to male sheep-derived MSCs. Cryopreserved MSCs have similar characteristics to fresh cells. Therefore, it is important to consider tissue type and donor physiological status when applying MSCs in clinical settings.
Article
Biochemistry & Molecular Biology
Lelia Bertoni, Sandrine Jacquet-Guibon, Thomas Branly, Melanie Desance, Florence Legendre, Martine Melin, Pascaline Rivory, Daniel-Jean Hartmann, Amandine Schmutz, Jean-Marie Denoix, Magali Demoor, Fabrice Audigie, Philippe Galera
Summary: This study evaluated the efficacy of allogeneic bone-marrow derived mesenchymal stem cells compared to umbilical cord blood derived MSCs in limiting the progression of OA-associated changes in horses. Results showed a significant reduction in OA-related changes with bone-marrow derived MSCs, suggesting promising therapeutic potential for OA in horses. Further research is needed to validate these results and discover innovative strategies for optimizing the therapeutic benefits of these cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Saleheh Jahani, Nabi Zare, Yousef Mirzaei, Reza Arefnezhad, Hooman Zarei, Pouya Goleij, Nader Bagheri
Summary: Ovarian cancer is a heterogeneous complex condition with high mortality, weak prognosis, and late-stage presentation. Current treatments have diverse side effects and limited effectiveness. The use of mesenchymal stem cells (MSCs) from various sources has gained attention for treating ovarian cancer. This review aims to discuss the effects of MSCs from different sources on ovarian cancer and their underlying mechanisms.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2023)
Article
Oncology
Amir K. Varkouhi, Xiaolin He, Ana Paula Teixeira Monteiro, Hajera Amatullah, James N. Tsoporis, Sahil Gupta, Amin M. Ektesabi, Shirley H. J. Mei, Duncan J. Stewart, Armand Keating, Claudia C. dos Santos
Summary: Comparative study on the therapeutic effects of bone marrow-derived MSCs (BM-MSCs) and umbilical cord-derived MSCs (UC-MSCs) in a mouse model of polymicrobial sepsis showed significant differences in gene expression profile, surface markers, and therapeutic potency between the two MSC types. BM-MSCs improved survival with enhanced bacterial clearance and improved inflammatory profile post CLP surgery, while UC-MSCs did not enhance survival or bacterial clearance despite some improvement in the inflammatory profile of septic animals.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Multidisciplinary Sciences
Romina Marcoccia, Salvatore Nesci, Barbara Merlo, Giulia Ballotta, Cristina Algieri, Alessandra Pagliarani, Eleonora Iacono
Summary: This study compared the characteristics of canine adipose tissue and umbilical cord matrix MSCs, finding no significant differences in cell proliferation, migration, spheroid formation ability, and differentiation potential. ATP production pathways were also evaluated, with mitochondrial respiration being the main pathway for both types of MSCs. Mitochondrial respiration and ATP turnover were higher in UC-MSCs compared to AT-MSCs, but both had 100% coupling efficiency, indicating a potential for differentiation.
Article
Cell & Tissue Engineering
Merline Benny, Benjamin Courchia, Sebastian Shrager, Mayank Sharma, Pingping Chen, Joanne Duara, Krystalenia Valasaki, Michael A. Bellio, Andreas Damianos, Jian Huang, Ronald Zambrano, Augusto Schmidt, Shu Wu, Omaida C. Velazquez, Joshua M. Hare, Aisha Khan, Karen C. Young
Summary: This study compared the regenerative effects of MSC obtained from bone marrow (BM) and umbilical cord tissue (UCT) in an experimental BPD model. The results showed that UCT-MSC had better proliferation and expression of anti-inflammatory cytokines, leading to better wound healing and reduced lung macrophage infiltration compared to BM-MSC.
STEM CELLS TRANSLATIONAL MEDICINE
(2022)
Article
Veterinary Sciences
Eva Punzon, Raquel Salguero, Xavier Totusaus, Cristian Mesa-Sanchez, Llorenc Badiella, Maria Garcia-Castillo, Almudena Pradera
Summary: The efficacy and safety of intra-articular administration of xenogeneic MSCs for the treatment of canine osteoarthritis were demonstrated in this study. Best results were observed at 8 weeks after treatment, with 63% of the patients showing improvement in gait analysis. Long-term follow-up showed that 59% of the owners reported a duration of effect longer than 6 months after a single intra-articular injection of equine umbilical cord MSCs. No systemic or permanent adverse events were detected.
JAVMA-JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION
(2022)
Article
Biochemistry & Molecular Biology
Maria Yoshida, Ayumu Nakashima, Naoki Ishiuchi, Kisho Miyasako, Keisuke Morimoto, Yoshiki Tanaka, Kensuke Sasaki, Satoshi Maeda, Takao Masaki
Summary: Both adipose-derived and bone marrow-derived mesenchymal stem cells (ADSCs and BMSCs) can suppress renal fibrosis, with ADSCs showing a stronger effect under serum-containing culture conditions. However, ADSC infusion in mice may lead to a higher death rate from pulmonary embolism. Therefore, the selection of ADSCs or BMSCs for clinical applications should consider the target disease and culture method.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Endocrinology & Metabolism
Ziru Li, Clifford J. Rosen
Summary: Bone marrow adipose tissue (BMAT) is a significant component of the bone marrow space in healthy adults, but its expansion is associated with aging, obesity, anorexia nervosa, and irradiation. While traditionally viewed as a negative component, recent studies have revealed that BMAT can serve as an energy reservoir and also plays a role in regulating bone formation and hematopoiesis. This review summarizes the unique characteristics of BMAT and provides an updated understanding of its physiological roles based on a newly established mouse model.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Review
Biochemistry & Molecular Biology
Victor J. Costela-Ruiz, Lucia Melguizo-Rodriguez, Chiara Bellotti, Rebeca Illescas-Montes, Deborah Stanco, Carla Renata Arciola, Enrico Lucarelli
Summary: The success of regenerative medicine depends on the appropriate selection of mesenchymal stem cell (MSC) sources, as they influence the regenerative milieu. MSCs from bone marrow, adipose tissue, and dental tissue are all potential candidates for oral and dental regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Hannah J. Stage, Susanne Trappe, Katharina Soellig, Dagmar S. Trachsel, Katharina Kirsch, Cornelia Zieger, Roswitha Merle, Joerg R. Aschenbach, Heidrun Gehlen
Summary: This study investigated the proliferation and multilineage differentiation potential of equine adipose stem cells (ASCs) obtained from different tissue sources. The study found that ASCs had high proliferation and differentiation potential, but were unable to undergo cardiomyogenic differentiation. This study provides a basis for future research in equine medicine.
Article
Multidisciplinary Sciences
Yu-Hee Kim, Hyun-Ji Lee, Kyung-Ah Cho, So-Youn Woo, Kyung-Ha Ryu
Summary: This study found that conditioned medium obtained from human tonsil-derived MSCs (T-MSC CM) can inhibit adipocyte differentiation by regulating glucocorticoid signal transduction, thereby reducing obesity. The experiments showed that T-MSC CM can attenuate early-stage adipocyte differentiation and protect mice from the negative effects induced by glucocorticoid treatment. Additionally, T-MSC CM enhances megakaryocyte differentiation and protects blood platelet counts.
Article
Chemistry, Physical
Camila Fernanda Zorzella Creste, Patricia Rodrigues Orsi, Fernanda Cruz Landim-Alvarenga, Luis Antonio Justulin, Marjorie de Assis Golim, Benedito Barraviera, Rui Seabra Ferreira
Article
Agriculture, Dairy & Animal Science
Thamiris Vieira Marsico, Jose Nelio de Sousa Sales, Christina Ramires Ferreira, Mateus Jose Sudano, Joao Henrique Moreira Viana, Luiz Sergio de Almeida Camargo, Marcos Nogueira Eberlin, Marcelo Marcondes Seneda, Pietro Sampaio Baruselli
Summary: This study aimed to characterize the lipid profiles of oocytes from different dairy cattle breeds (Bos indicus, Bos taurus, and their crossbreed), and revealed distinct lipid profile patterns between Gir and Holstein, while Gir and crossbreed oocytes showed greater similarities. The predominant lipids identified were phospholipids in Gir (Bos indicus) oocytes and triacylglycerol in Holstein (Bos taurus) oocytes.
Article
Reproductive Biology
Roniele Santana Valente, Tamie Guibu de Almeida, Mayra Fernanda Alves, Daniela Martins Paschoal, Andrea Cristina Basso, Mateus Jose Sudano
Article
Biochemistry & Molecular Biology
Franciele Lanzarini, Fernanda Alves Pereira, Janine de Camargo, Andressa Minozzo Oliveira, Katia Roberta Anacleto Belaz, Jose Javier Melendez-Perez, Marcos Nogueira Eberlin, Mario Celso Sperotto Brum, Fernando Silveira Mesquita, Mateus Jose Sudano
Summary: The translational blockage of ELOVL5 affects embryonic lipid content and composition, leading to downregulation of specific lipid species and increased cytoplasmic lipid droplet deposition. However, it does not have detrimental effects on embryonic development and blastocyst cell number.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Diego Noe Rodriguez-Sanchez, Giovana Boff Araujo Pinto, Luciana Politti Cartarozzi, Alexandre Leite Rodrigues de Oliveira, Ana Livia Carvalho Bovolato, Marcio de Carvalho, Jorge Vicente Lopes da Silva, Janaina de Andrea Dernowsek, Marjorie Golim, Benedito Barraviera, Rui Seabra Ferreira, Elenice Deffune, Mathues Bertanha, Rogerio Martins Amorim
Summary: The study demonstrated that the combination of 3D-printed PLC-NGCs with AdMSCs derived from adipose tissue exerted neuroregenerative effects on rats with sciatic nerve injury, improving motor function and nerve conduction.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Chemistry, Medicinal
Eliza de Oliveira Cardoso, Karina Basso Santiago, Bruno Jose Conti, Fernanda Lopes Conte, Karen Ingrid Tasca, Graziela Gorete Romagnoli, Marjorie de Assis Golim, Claudia Aparecida Rainho, Jairo Kenupp Bastos, Jose Mauricio Sforcin
Summary: The study found that the ethanol solution extract of propolis (EEP) in combination with Docetaxel (DTX) enhances cytotoxicity against breast cancer cells while exerting no cytotoxic effects on monocytes. The combination also stimulates HLA-DR expression in monocytes, increases cytokine production, enhances antifungal activity, and reduces oxidative stress. These findings have practical implications and provide new insights for complementary medicine.
PHYTOTHERAPY RESEARCH
(2022)
Article
Behavioral Sciences
Felipe Jacques Sanches, Jessica Cardia de Melo, Sabrina Palha Ferreira, Luzia Aparecida Trinca, Omar Gurrola Arambula, Flavia Helena Pereira Padovani, Arthur Oscar Schelp, Rogerio Martins Amorim
Summary: Episodic memory in elderly dogs declines similar to humans, regardless of gender and size, which may be related to the physiological aging process or preclinical pathological manifestation of cognitive impairment. Further studies are needed to evaluate episodic-like memory in dogs with canine cognitive dysfunction syndrome to better understand its physiological and pathological behavior in canine species.
BEHAVIOURAL BRAIN RESEARCH
(2022)
Article
Environmental Sciences
Helga Caputo Nunes, Samara Costa Tavares, Heloisa Vicente Garcia, Maira Smaniotto Cucielo, Sergio Alexandre Alcantara dos Santos, Mirian Carolini Esgoti Aal, Marjorie Assis de Golim, Luis Antonio Justulin Jr, Amanda Oliveira Ribeiro, Elenice Deffune, Wellerson Rodrigo Scarano, Flavia Karina Delella
Summary: Chemical contamination is an important factor to consider in cell therapy strategy design. This study found that BPA and TCDD can significantly reduce cell viability and affect adipogenic differentiation and DNA damage.
ENVIRONMENTAL TOXICOLOGY
(2022)
Article
Veterinary Sciences
Lorena Battistin, Fabricio Moreira Cerri, Marcos Jun Watanabe, Regina Kiomi Takahira, Marcio Garcia Ribeiro, Noeme Sousa Rocha, Jose Paes de Oliveira-Filho, Alexandre Secorun Borges, Rogerio Martins Amorim
Summary: The clinical findings of pleural empyema in six horses were studied retrospectively. The horses showed various clinical signs, including tachycardia, tachypnea, cyanotic mucosa, hyperthermia, dyspnea, fluid presence, and coarse crackling on auscultation. Blood tests revealed leukocytosis, neutrophilia, hyperfibrinogenemia, and increased urea and globulins. Pathogens such as Aspergillus fumigatus and Streptococcus were isolated from transtracheal wash and pleural effusion. The fatality rate was 83% and post-mortem examination revealed fibrin in the pleural cavity with adhesion between the parietal and visceral pleura. Pleural empyema is a complex disease that is difficult to treat conventionally.
JOURNAL OF EQUINE VETERINARY SCIENCE
(2023)
Article
Veterinary Sciences
Cristiana R. Bromberger, Joao Pedro M. Oliveira, Ana Maria D. Costa, Rogerio M. Amorim, Alexandre S. Borges, Jose P. Oliveira-Filho
Summary: This study aimed to describe the epidemiological and clinical signs of cattle cases treated at the Large Animal Hospital in the midwestern region of Sao Paulo state. A retrospective analysis of 638 clinical cases treated from January 2010 to December 2019 was conducted. Digestive system diseases were the most prevalent, followed by neurological and respiratory diseases. Rabies was the main cause of death and neurological signs in cattle.
PESQUISA VETERINARIA BRASILEIRA
(2023)
Article
Veterinary Sciences
Lucas V. O. Ferreira, Maria L. G. Lourenco, Regina K. Takahira, Juliana M. Alonso, Jose P. Oliveira-Filho, Alexandre S. Borges, Simone B. Chiacchio, Rogerio M. Amorim
Summary: Vagal indigestion (VI) is a digestive disorder characterized by dysfunction of the vagus nerve. This study analyzed clinical and laboratory aspects of mini-cattle diagnosed with VI. The predominant clinical manifestations were gas accumulation, abdominal distension, decreased rumen motility, and pasty stools. Treatment included ruminal fistulation and implantation of a permanent rumen cannula.
PESQUISA VETERINARIA BRASILEIRA
(2023)
Article
Veterinary Sciences
Fabricio Moreira Cerri, Isabella Mendonca Zanella Cecconi Cardoso, Vania Maria de Vasconcelos Machado, Jose Paes de Oliveira-Filho, Rogerio Martins Amorim, Alexandre Secorun Borges, Danilo Giorgi Abranches de Andrade
Summary: This study aimed to describe the clinical signs and MRI findings in a sheep with PEM. Thiamine therapy proved to be effective and capable of reverting the clinical signs. MRI can be used as a useful tool for the ante-mortem diagnosis of PEM.
ACTA SCIENTIAE VETERINARIAE
(2022)
Article
Veterinary Sciences
Lukas G. Albertino, Natalia M. Dias, Juliana J. Moreira, Jose P. Oliveira-Filho, Regina K. Takahira, Renee Laufer-Amorim, Alexandre S. Borges, Rogerio M. Amorim
Summary: Equine leukoencephalomalacia is a disease caused by ingesting fumonisin-contaminated food, mainly affecting horses. Clinical signs include acute onset and rapid evolution of brain injuries, with altered mental state and behavioral disturbance as the main symptoms.
PESQUISA VETERINARIA BRASILEIRA
(2021)
Article
Cell & Tissue Engineering
Felipe Perez Benavides, Giovana Boff Araujo Pinto, Marta Cristina Thomas Heckler, Diana Milena Rodriguez Hurtado, Livia Ramos Teixeira, Marina Mitie de Souza Monobe, Gisele Fabrino Machado, Guilherme Dias de Melo, Diego Noe Rodriguez-Sanchez, Fernanda da Cruz Landim e Alvarenga, Rogerio Martins Amorim
Summary: The study demonstrates that intrathecal transplantation of BM-MSCs in dogs provides a safe, easy, and minimally invasive route for the use of cell-based therapeutics in central nervous system diseases.
CELL TRANSPLANTATION
(2021)
Article
Biochemistry & Molecular Biology
Thamiris Marsico, Diana P. Caetano, Rafaela Rodrigues, Roniele S. Valente, Patricia K. Fontes, Fernando S. Mesquita, Sonia C. da Silva Andrade, Andrea C. Basso, Marcelo F. G. Nogueira, Mateus J. Sudano
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2020)