Journal
STEM CELL RESEARCH & THERAPY
Volume 5, Issue -, Pages -Publisher
BMC
DOI: 10.1186/scrt435
Keywords
-
Funding
- Deutsche Forschungsgemeinschaft DFG [ED79/1-2]
- German Ministry of Education and Research, BMBF [01 GN 0813]
- Merck KGaA, Darmstadt, Germany
Ask authors/readers for more resources
Integrating viruses represent robust tools for cellular reprogramming; however, the presence of viral transgenes in induced pluripotent stem cells (iPSCs) is deleterious because it holds the risk of insertional mutagenesis leading to malignant transformation. Here, we combine the robustness of lentiviral reprogramming with the efficacy of Cre recombinase protein transduction to derive iPSCs devoid of transgenes. By genome-wide analysis and targeted differentiation towards the cardiomyocyte lineage, we show that transgene-free iPSCs are superior to iPSCs before Cre transduction. Our study provides a simple, rapid and robust protocol for the generation of clinical-grade iPSCs suitable for disease modeling, tissue engineering and cell replacement therapies.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available