Article
Immunology
Yang Ji, Peihong Wang, Tingting Xu, Yanzi Zhou, Rongchang Chen, Huaiqiu Zhu, Kai Zhou
Summary: A study aimed to develop a method to distinguish four epidemiologically prevalent species of Enterobacter cloacae complex (ECC) with clinical significance has successfully achieved a high specificity and sensitivity in species-specific PCR, especially for E. cloacae. The established multiplex PCR provides an accurate, expeditious, and cost-effective way for routine diagnosis and molecular surveillance of ECC strains at species level.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Environmental Sciences
Shilin Tan, Shixuan Huang, Zekun Liu, Moutong Chen, Hanfang Chen, Qinghua Ye, Shi Wu, Xiaojuan Yang, Shuhong Zhang, Jumei Zhang, Qingping Wu, Meiyan Yang
Summary: In this study, a phage named Q7622 was isolated from the sewage of Jiangcun poultry market in Guangzhou city, which can effectively target multidrug-resistant bacteria without known virulence and resistance genes. Comparative genomic and phylogenetic analysis revealed that Q7622 is a novel Enterobacter cloacae phage strain of the genus Kanagawavirus.
FOOD AND ENVIRONMENTAL VIROLOGY
(2023)
Article
Immunology
Sabrina Klein, Sebastien Boutin, Isabel Spaeth, Christoph Kimmich, Juliane Brandt, Carsten Mueller-Tidow, Klaus Heeg, Dennis Nurjadi
Summary: The study highlights a transmission cluster of bla(KPC-2)-positive Enterobacter cloacae among patients treated in a highly frequented outpatient department, underscoring the potential role of outpatient clinics as transmission grounds for multidrug-resistant organisms.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Biotechnology & Applied Microbiology
Yingying Xia, Yufeng Xu, Yiling Zhou, Yanyan Yu, Yinhua Chen, Chunxia Li, Wei Xia, Jun Tao
Summary: In this study, a highly Cd-resistant strain, LPY6, was obtained through domestication of Enterobacter cloacae strain Cu6. LPY6 was found to alleviate the toxic effects of Cd on wheat seedling growth. The high Cd-resistance mechanism of LPY6 was deciphered through genome comparative and genetic analysis, revealing the involvement of mutated genes, including cadA and norVW, in Cd resistance in E. cloacae.
INTERNATIONAL MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Min Liao, Yixin Luo, Na Xu, Xiaomei Xie, Xinjun Gan, Dan Cao
Summary: A heterotrophic nitrifier and aerobic denitrifier, identified as Enterobacter cloacae DK-6, was isolated from chaff bedding of a pig farm. Strain DK-6 showed efficient nitrogen removal abilities under aerobic condition, achieving removal efficiencies of 86.98%, 94.66%, and 100% for ammonium-N, nitrate-N, and nitrite-N, respectively. The study also found that strain DK-6 preferred to utilize nitrite-N when mixed nitrogen sources were present, leading to improved nitrate-N removal efficiency. The results suggested that strain DK-6 has significant potential in treating multiple nitrogen pollutants in wastewater under aerobic condition and lower carbon/nitrogen ratio.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2022)
Article
Microbiology
Xu Dong, Mei Zhu, Yaxuan Li, Dawei Huang, Lan Wang, Chunxia Yan, Linhua Zhang, Fubo Dong, Junwan Lu, Xi Lin, Kewei Li, Qiyu Bao, Cheng Cong, Wei Pan
Summary: In this study, 172 clinical isolates of Enterobacter cloacae complex (ECC) were collected from three hospitals in Zhejiang, China between 2019 and 2020. Whole-genome sequencing was performed to analyze the population structure and drug resistance mechanisms. The majority of isolates belonged to E. hormaechei and E. kobei, and resistance genes were mainly associated with beta-lactam antibiotics. Species-specific genetic differences and the presence of carbapenemase-encoding genes in an E. hormaechei isolate were also identified. Overall, genome-wide molecular characterization provides important insights into the ECC population and resistance mechanisms.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Dennis Nurjadi, Martin Scherrer, Uwe Frank, Nico T. Mutters, Alexandra Heininger, Isabel Spaeth, Vanessa M. Eichel, Jonas Jabs, Katja Probst, Carsten Mueller-Tidow, Juliane Brandt, Klaus Heeg, Sebastien Boutin
Summary: The hospital water environment can serve as a reservoir for multiward outbreaks, leading to acquisitions or transmissions of multidrug-resistant organisms in a hospital setting. Most Gram-negative bacteria have the ability to form biofilms and persist in hospital plumbing systems for extended periods. Eliminating these reservoirs is crucial to preventing further transmission, but proposed decontamination regimens, such as using acetic acid, can only suppress rather than fully eliminate the environmental reservoir. This study demonstrated that colonization with multidrug-resistant organisms can be acquired through showering in showers with contaminated water traps and drains.
MICROBIOLOGY SPECTRUM
(2021)
Article
Environmental Sciences
Xiaobo Wang, Zhansheng Wu, Huichun Xiang, Yanhui He, Shuangxi Zhu, Ziyan Zhang, Xueping Li, Jianwen Wang
Summary: This study sequenced and analyzed the whole genome of Enterobacter cloacae Rs-2, and found its potential in improving crop growth. The application of Rs-2 as a soil inoculant in pot experiments showed great success. This study provides guidance and a foundation for further improvement and application of this strain in agricultural production.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Infectious Diseases
Matus Stefanek, Sigurd Wenner, Vitor Borges, Miguel Pinto, Joao Paulo Gomes, Joao Rodrigues, Isabel Faria, Maria Ana Pessanha, Filomena Martins, Raquel Sabino, Cristina Verissimo, Isabel D. Nogueira, Patricia Almeida Carvalho, Helena Bujdakova, Luisa Jordao
Summary: This study evaluated the biofilm formation and antimicrobial resistance of Enterobacter cloacae complex and Candida parapsilosis co-isolated from a catheter-related bloodstream infection (CRBSI) patient. The bacteria showed multidrug resistance, including resistance to colistin and beta-lactams. The Candida parapsilosis exhibited resistance to fluconazole. The nature of biofilms varied depending on the material and the pathogens involved, and the distribution of microorganisms differed on the inner and outer surface of the central venous catheter.
Article
Food Science & Technology
Fang Liu, Panpan Jin, Zhilan Sun, Lihui Du, Daoying Wang, Tong Zhao, Michael P. Doyle
Summary: This study showed that carvacrol oil inhibits biofilm formation and EPS production of Enterobacter cloacae by down-regulating multiple genes related to biofilm formation. Additionally, the use of other antimicrobial agents can further reduce the number of bacterial cells in carvacrol-treated biofilms.
Article
Microbiology
Tripti Misra, Meghana Tare, Prabhat Nath Jha
Summary: This study provides a detailed characterization of the biofilm formation process of E. cloacae SBP-8 on different surfaces. The results show that medical devices are highly colonized by this bacterium. The study also investigates the formation and composition of the biofilm using microscopy and spectroscopic techniques.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Environmental Sciences
Alireza Mohammadzadeh, Mustafa M. Kadhim, Talib Zeedan Taban, Omirserik Baigenzhenov, Andrei Ivanets, Basant Lal, Naveen Kumar, Ahmad Hosseini-Bandegharaei
Summary: In this study, the adsorption properties of Enterobacter cloacae towards uranium were investigated. The results showed that Enterobacter cloacae has a high affinity for fast and selective biosorption of uranium ions. Additionally, a composite material of Enterobacter cloacae/carbon nanotube was synthesized and utilized for preconcentration and determination of uranium, demonstrating low detection limit, high precision, and good accuracy.
Article
Immunology
Muhammad Asif, Hafsa Naseem, Iqbal Ahmad Alvi, Abdul Basit, Shafiq-Ur-Rehman
Summary: The study isolated and characterized the lytic bacteriophage EBP against Enterobacter cloacae, which showed stable antibacterial activity under various environmental conditions and a narrow host range. The genome of EBP contains necessary enzymes and proteins for lysis. Phylogenetic analysis revealed similarities of EBP with certain Coronobacter and Citrobacter phages, classifying EBP as a Myoviridae phage based on electron microscopy and molecular characterization.
Article
Multidisciplinary Sciences
William Bennett, Katrin Mende, Wesley R. Campbell, Miriam Beckius, Laveta Stewart, Faraz Shaikh, Azizur Rahman, David R. Tribble, Joseph M. Yabes
Summary: This study evaluated the characteristics, antibiotic utilization, and outcomes of battlefield-related Enterobacter cloacae infections. Patients with E. cloacae infections sustained more blast trauma, required more initial blood products, and had longer durations of antibiotic therapy and hospitalizations compared to patients with non-E. cloacae infections.
Editorial Material
Biochemistry & Molecular Biology
Zhiyong Zong, Yu Feng, Alan McNally
Summary: Enterobacter is a globally important pathogen with resistance to carbapenem and colistin. Further research is needed to understand colistin heteroresistance and the emergence of certain Enterobacter lineages clinically.
TRENDS IN MICROBIOLOGY
(2021)
Article
Microbiology
Brenda K. Schroeder, William L. Schneider, Douglas G. Luster, Aaron Sechler, Timothy D. Murray
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2018)
Article
Plant Sciences
Bianca L. Barrantes-Infante, Brenda K. Schroeder, Sergei A. Subbotin, Timothy D. Murray
Article
Microbiology
Edward W. Davis, Javier F. Tabima, Alexandra J. Weisberg, Lucas Dantas Lopes, Michele S. Wiseman, Michael S. Wiseman, Tal Pupko, Michael S. Belcher, Aaron J. Sechler, Matt A. Tancos, Brenda K. Schroeder, Timothy D. Murray, Douglas G. Luster, William L. Schneider, Elizabeth E. Rogers, Fernando D. Andreote, Niklaus J. Grunwald, Melodie L. Putnam, Jeff H. Chang
Article
Mathematical & Computational Biology
Lisa Harper, Jacqueline Campbell, Ethalinda K. S. Cannon, Sook Jung, Monica Poelchau, Ramona Walls, Carson Andorf, Elizabeth Arnaud, Tanya Z. Berardini, Clayton Birkett, Steve Cannon, James Carson, Bradford Condon, Laurel Cooper, Nathan Dunn, Christine G. Elsik, Andrew Farmer, Stephen P. Ficklin, David Grant, Emily Grau, Nic Herndon, Zhi-Liang Hu, Jodi Humann, Pankaj Jaiswal, Clement Jonquet, Marie-Angelique Laporte, Pierre Larmande, Gerard Lazo, Fiona McCarthy, Naama Menda, Christopher J. Mungall, Monica C. Munoz-Torres, Sushma Naithani, Rex Nelson, Daureen Nesdill, Carissa Park, James Reecy, Leonore Reiser, Lacey-Anne Sanderson, Taner Z. Sen, Margaret Staton, Sabarinath Subramaniam, Marcela Karey Tello-Ruiz, Victor Unda, Deepak Unni, Liya Wang, Doreen Ware, Jill Wegrzyn, Jason Williams, Margaret Woodhouse, Jing Yu, Doreen Main
DATABASE-THE JOURNAL OF BIOLOGICAL DATABASES AND CURATION
(2018)
Article
Biochemistry & Molecular Biology
Sook Jung, Taein Lee, Chun-Huai Cheng, Katheryn Buble, Ping Zheng, Jing Yu, Jodi Humann, Stephen P. Ficklin, Ksenija Gasic, Kristin Scott, Morgan Frank, Sushan Ru, Heidi Hough, Kate Evans, Cameron Peace, Mercy Olmstead, Lisa W. DeVetter, James McFerson, Michael Coe, Jill L. Wegrzyn, Margaret E. Staton, Albert G. Abbott, Dorrie Main
NUCLEIC ACIDS RESEARCH
(2019)
News Item
Plant Sciences
T. D. Murray, B. Barrantes-Infante, B. K. Schroeder
Editorial Material
Plant Sciences
J. W. Woodhall, M. Harrington, S. Keith, A. Oropeza, M. Thornton, B. Schroeder
Editorial Material
Plant Sciences
K. D. Beck, C. Reyes-Corral, M. Rodriguez-Rodriguez, C. May, R. Barnett, M. K. Thornton, A. A. Bates, J. W. Woodhall, B. K. Schroeder
Article
Chemistry, Analytical
Gajanan S. Kothawade, Sindhuja Sankaran, Austin A. Bates, Brenda K. Schroeder, Lav R. Khot
Article
Plant Sciences
Ann M. Callahan, Tetyana N. Zhebentyayeva, Jodi L. Humann, Christopher A. Saski, Kelsey D. Galimba, Laura L. Georgi, Ralph Scorza, Dorrie Main, Christopher D. Dardick
Summary: The 'HoneySweet' plum is resistant to Plum pox potyvirus through an RNAi-triggered mechanism. Whole genome sequencing revealed two different transgene insertion events, but the specific locations of the insertions remain undetermined due to potential sequence differences between the two plum cultivars. RNA-seq data confirmed that the insertions did not disrupt any genes or cause significant expression changes in neighboring genes.
HORTICULTURE RESEARCH
(2021)
Article
Plant Sciences
Jing Yu, Sook Jung, Chun-Huai Cheng, Taein Lee, Ping Zheng, Katheryn Buble, James Crabb, Jodi Humann, Heidi Hough, Don Jones, J. Todd Campbell, Josh Udall, Dorrie Main
Summary: Over the past eight years, the volume of data generated by the cotton research community has exponentially increased, leading to the creation of the CottonGen online community database which provides access to integrated genetic and breeding data and analysis tools. The data stored in CottonGen includes genes, genotypes, and phenotypes, and the platform facilitates utilization of these resources for research on cotton crop improvement through curation and online tools.
Article
Plant Sciences
Lauren E. Braley, Jeremy B. Jewell, Jose Figueroa, Jodi L. Humann, Dorrie Main, Guadalupe A. Mora-Romero, Natalia Moroz, James W. Woodhall, Richard Allen White, Kiwamu Tanaka
Summary: Early detection of causal pathogens is crucial for preventing crop loss. This study assessed the use of the Oxford Nanopore MinION sequencer as a molecular diagnostic tool for soilborne diseases in potato fields. The results demonstrated the high potential of Nanopore sequencing as a minimally biased method for comprehensive pathogen detection. The GraphMap-based MinION sequencing method could contribute to disease management by identifying pathogens in field soil prior to planting.
Article
Mathematical & Computational Biology
Sook Jung, Taein Lee, Ksenija Gasic, B. Todd Campbell, Jing Yu, Jodi Humann, Sushan Ru, Daniel Edge-Garza, Heidi Hough, Dorrie Main
Summary: In the era of big data, efficient management systems like the Breeding Information Management System (BIMS) are essential for breeders to keep track of and analyze the large amounts of data generated by breeding programs. BIMS allows breeders to integrate their private breeding data with public data, enhancing genetic understanding and utility for marker-assisted breeding. The system also includes tools like the Android App Field Book for easier data collection and analysis.
DATABASE-THE JOURNAL OF BIOLOGICAL DATABASES AND CURATION
(2021)
Article
Mathematical & Computational Biology
Sook Jung, Chun-Huai Cheng, Katheryn Buble, Taein Lee, Jodi Humann, Jing Yu, James Crabb, Heidi Hough, Dorrie Main
Summary: Tripal MegaSearch is a module for querying and downloading biological data stored in Chado, allowing users to select data types and customize fields. Administrators can set example data types, while users can restrict datasets and download desired data accordingly.
DATABASE-THE JOURNAL OF BIOLOGICAL DATABASES AND CURATION
(2021)
Article
Mathematical & Computational Biology
Katheryn Buble, Sook Jung, Jodi L. Humann, Jing Yu, Chun-Huai Cheng, Taein Lee, Stephen P. Ficklin, Heidi Hough, Bradford Condon, Margaret E. Staton, Jill L. Wegrzyn, Dorrie Main
DATABASE-THE JOURNAL OF BIOLOGICAL DATABASES AND CURATION
(2019)
