Article
Biology
Alejandro Gimeno, Claire E. Stanley, Zacharie Ngamenie, Ming-Hui Hsung, Florian Walder, Stefanie S. Schmieder, Saskia Bindschedler, Pilar Junier, Beat Keller, Susanne Vogelgsang
Summary: Researchers have developed a microfluidic platform for real-time measurement of fungal-fungal interactions at the hyphal level. This device uses microchannel geometry to enhance the visibility of hyphal growth and provides control channels for comparisons between localized and systemic effects, facilitating the study of the relationship between the pathogenic fungus Fusarium graminearum and the biological control agent Clonostachys rosea.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Chang-Kwon Kim, Lauren R. H. Krumpe, Emily Smith, Curtis J. Henrich, Isaac Brownell, Karen L. Wendt, Robert H. Cichewicz, Barry R. O'Keefe, Kirk R. Gustafson
Summary: A new linear peptide named roseabol A (1) and a known compound 13-oxo-trans-9,10-epoxy-11(E)-octadecenoic acid (2) were isolated from the fungus Clonostachys rosea. Roseabol A (1) contains amino acid residues characteristic of the peptaibol family of peptides, while compound 2 showed inhibitory activity against Merkel cell carcinoma.
Article
Microbiology
Zhan-Bin Sun, Shu-Fan Yu, Man-Hong Sun, Shi-Dong Li, Ya-Feng Hu, Han-Jian Song
Summary: This study sequenced and analyzed the transcriptome of C. rosea mycoparasitizing a Basidiomycota fungus, R. solani, and identified several mycoparasitism-related genes, including ABC transporter-, glucanase- and chitinase-encoding genes. Comparative transcriptomic analysis with S. sclerotiorum revealed unique expression of several genes in C. rosea mycoparasitizing R. solani.
Article
Biotechnology & Applied Microbiology
Edoardo Piombo, Ramesh Raju Vetukuri, Poorva Sundararajan, Sandeep Kushwaha, Dan Funck Jensen, Magnus Karlsson, Mukesh Dubey
Summary: In this study, the role of sRNA-mediated gene regulation in mycoparasitic interactions was investigated by performing sRNA and degradome tag sequencing of the mycoparasitic fungus Clonostachys rosea interacting with the plant-pathogenic mycohosts Botrytis cinerea and Fusarium graminearum. The majority of differentially expressed sRNAs were downregulated during the interactions with the mycohosts compared to a C. rosea self-interaction control, thus allowing desuppression of mycohost-responsive genes. Degradome analysis showed a positive correlation between high degradome counts and antisense sRNA mapping and led to the identification of 201 sRNA-mediated potential gene targets for 282 differentially expressed sRNAs. The study expands the understanding of sRNA in regulating interspecific fungal interactions and mycoparasitism.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Rakibul Hasan, Binna Lv, Md Jamal Uddin, Yingying Chen, Lele Fan, Zhanbin Sun, Manhong Sun, Shidong Li
Summary: Clonostachys rosea is an important mycoparasite with potential for controlling plant fungal diseases. This study assessed the interactions between C. rosea and Botrytis cinerea during co-culture and encounter on tomato leaves. The results showed that C. rosea can parasitize host plants and disintegrate cells of the pathogen by absorbing nutrients.
Article
Agronomy
Vladimir Mironov, Anna Shchelushkina, Olga Selitskaya, Yury Nikolaev, Alexander Merkel, Shenghua Zhang
Summary: This study examines the impact of introducing autochthonous compost species on the phytopathogen-suppressive and phytostimulation activity of composts. The results show that the introduction of autochthonous bacterial-fungal composition can improve the fertilizing properties of compost and exhibit strong inhibitory activity against fungal phytopathogens.
Article
Plant Sciences
Alfia Khairullina, Nikola Micic, Hans J. Lyngs Jorgensen, Nanna Bjarnholt, Leif Bulow, David B. Collinge, Birgit Jensen
Summary: The fungal biocontrol agent Clonostachys rosea is effective in reducing Fusarium graminearum DNA and DON toxin accumulation in oat, and can activate resistance-related gene expression. These findings suggest that C. rosea has potential as a biocontrol agent against Fusarium head blight in oat.
Article
Biochemistry & Molecular Biology
Zerihun A. Demissie, Kelly A. Robinson, Michele C. Loewen
Summary: The Clonostachys rosea strains ACM941 and 88-710 share a high degree of genetic similarity, with over 96% of their genomes being encoded in a similar manner. This genetic sequence provides a foundation for further studies on the beneficial properties of these strains.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Article
Microbiology
Sahimerdan Turkolmez, Goksel Ozer, Sibel Dervis
Summary: In this study, the endophytic ability and growth effect of Clonostachys rosea strain ST1140 on solanaceous plants were tested. The study also investigated different organic substrates for cultivating and multiplying the strain. The results showed that inoculating seeds with ST1140 resulted in faster and better seed germination and plant growth compared to non-inoculated controls.
CURRENT MICROBIOLOGY
(2023)
Article
Microbiology
Abhishek Kumar Tamta, Renu Pandey, Jiten R. Sharma, Rajnish Rai, Mritunjoy Barman, M. G. Deeksha, Debasis Mitra, Pradeep Kumar Das Mohapatra, Rokayya Sami, Amina A. M. Al-Mushhin, Fadi Baakdah, Yasser S. Mostafa, Sulaiman A. Alrumman, Mahmoud Helal
Summary: Mango hopper (Amritodus atkinsoni Lethierry) causes devastating damage to mango crops during the early vegetative stage. This study reveals the potential entomopathogenic role of Clonostachys rosea in controlling A. atkinsoni and provides optimized media for its large-scale culturing.
Article
Microbiology
Kelly A. Robinson, Antony D. St-Jacques, Sam W. Shields, Amanda Sproule, Zerihun A. Demissie, David P. Overy, Michele C. Loewen
Summary: A study found that a fungus called Clonostachys rosea is able to convert 15-acetyl-deoxynivalenol (15-ADON) into 15-acetyl-deoxynivalenol-3-O-glycoside (15-ADON-3G). By knocking out UDP-GTases genes in C. rosea, it was discovered that multiple UDP-GTase enzymes are involved in this conversion process. These findings contribute to our understanding of the functions and relationships of UDP-GTases in fungi and plants.
Article
Zoology
Zhiyuan Yang, Qijing Wu, Jinlan Fan, Jierong Huang, Zhongzhen Wu, Jintian Lin, Shuying Bin, Benshui Shu
Summary: The Asian citrus psyllid is a serious pest of citrus that also transmits the devastating citrus disease Huanglongbing. Clonostachys rosea, a versatile fungus, shows potential as a biological control agent against ACP adults. Comparative transcriptomic analyses revealed genes involved in immunity, apoptosis, and cuticle formation that were differentially expressed after treatment with C. rosea, indicating its virulence against ACP adults.
JOURNAL OF INVERTEBRATE PATHOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Lin Wang, Anna-Lene Kiffe-Delf, Philipp Niklas Ostermann, Viktor Emanuel Simons, Di He, Ying Gao, Lasse van Geelen, Hao-Fu Dai, You-Xing Zhao, Heiner Schaal, Attila Mandi, Sandor Balazs Kiraly, Tibor Kurtan, Zhen Liu, Rainer Kalscheuer
Summary: Clonostachys rosea is a fungus that is widely distributed and has a high capacity to adapt to complex environments in soil, plants, or sea. It can be used as a biocontrol agent to protect plants from pathogenic fungi, nematodes, and insects. However, the secondary metabolites produced by C. rosea have been scarcely studied.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Maryam Elfeki, Shrikant Mantri, Chase M. Clark, Stefan J. Green, Nadine Ziemert, Brian T. Murphy
Summary: Environmental microorganisms are a significant source of bioactive natural products, but there is no apparent geographic distribution pattern for these products across different sampling locations, indicating a high degree of chemical diversity between sediment microbial communities.
ACS CHEMICAL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Rong Chen, Tianyuan Hu, Ming Wang, Yuhan Hu, Shu Chen, Qiuhui Wei, Xiaopu Yin, Tian Xie
Summary: This study analyzed the metabolites of Curcuma wenyujin's leaf and tuber tissues, identifying 11 curcuminoid metabolites and revealing the biosynthesis pathway. Two candidate type III polyketide synthases (PKSs) were identified, with CwPKS1 being the first one capable of synthesizing hydrogenated derivatives of curcuminoids. Notably, a mutation (G219D) resulted in the complete inactivation of CwPKS1.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2022)
Article
Evolutionary Biology
Martin Broberg, Mukesh Dubey, Mudassir Iqbal, Mikael Gudmundssson, Katarina Ihrmark, Hans-Josef Schroers, Dan Funck Jensen, Mikael Brandstrom Durling, Magnus Karlsson
Summary: This study analyzed the genome sequences of 11 Clonostachys strains and identified gene families evolving under selection for gene gains or losses. The results showed that gene families involved in drug efflux and biosynthesis of secondary metabolites are under evolutionary pressure in Clonostachys.
EVOLUTIONARY APPLICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Mukesh Dubey, Dan Funck Jensen, Magnus Karlsson
Summary: The study characterized the biological function of the aegerolysin gene agl1 in the mycoparasitic fungus Trichoderma atroviride, revealing its involvement in conidiation and antagonism. The data demonstrate the role of AGL1 in conidiation and antagonism in the mycoparasitic fungus T. atroviride.
MOLECULAR GENETICS AND GENOMICS
(2021)
Article
Microbiology
Edoardo Piombo, Ramesh R. Vetukuri, Anders Broberg, Pruthvi B. Kalyandurg, Sandeep Kushwaha, Dan Funck Jensen, Magnus Karlsson, Mukesh Dubey
Summary: This study investigated the role of DCL-dependent RNAi in mycoparasitic interactions by deleting dcl genes in Clonostachys rosea, revealing the importance of DCL in producing sRNAs involved in antagonistic actions. The study also identified novel microRNA-like RNAs in C. rosea and demonstrated potential cross-species communication mediated by sRNAs targeting virulence factors in other fungi.
MICROBIOLOGY SPECTRUM
(2021)
Article
Mycology
Beatriz Gomez-Munoz, Aikaterini Efthymiou, Mukesh Dubey, Jonathan Solve, Mette Nicolaisen, Dan F. Jensen, Ole Nybroe, John Larsen
Summary: The successful application of microbial biofertilizers is hindered by the lack of knowledge about their ecology. A study found that the addition of cellulose as a carbon source promotes the growth and phosphorus solubilization of Penicillium aculeatum, particularly in non-sterile soil.
Article
Agronomy
Carol Kalin, Anna Berlin, Agnese Kolodinska Brantestam, Mukesh Dubey, Anna-Kerstin Arvidsson, Paul Riesinger, Malin Elfstrand, Magnus Karlsson
Summary: This study investigated the genetic diversity of the oomycete pathogen Aphanomyces euteiches, which causes root rot in pea plants, in different countries in Europe. The results showed that strains from Italy were genetically distinct from strains in other countries, and strains from Finland and the eastern parts of Sweden were significantly different from other populations. However, although there were genetic differences between strains, there was no clear correlation between genetic relatedness and virulence levels.
Review
Agronomy
David B. Collinge, Dan Funck Jensen, Mojgan Rabiey, Sabrina Sarrocco, Michael W. Shaw, Richard H. Shaw
Summary: The global demand for sustainable agriculture is driving the use of biological control in crop disease management. Biological control using antagonistic microorganisms can complement other sustainable disease management practices and address diseases that are resistant to or lack other control methods. The prospects for biological control are promising, providing environmental protection and improving crop yield and quality.
Article
Biotechnology & Applied Microbiology
Edoardo Piombo, Ramesh Raju Vetukuri, Poorva Sundararajan, Sandeep Kushwaha, Dan Funck Jensen, Magnus Karlsson, Mukesh Dubey
Summary: In this study, the role of sRNA-mediated gene regulation in mycoparasitic interactions was investigated by performing sRNA and degradome tag sequencing of the mycoparasitic fungus Clonostachys rosea interacting with the plant-pathogenic mycohosts Botrytis cinerea and Fusarium graminearum. The majority of differentially expressed sRNAs were downregulated during the interactions with the mycohosts compared to a C. rosea self-interaction control, thus allowing desuppression of mycohost-responsive genes. Degradome analysis showed a positive correlation between high degradome counts and antisense sRNA mapping and led to the identification of 201 sRNA-mediated potential gene targets for 282 differentially expressed sRNAs. The study expands the understanding of sRNA in regulating interspecific fungal interactions and mycoparasitism.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Soil Science
Yvonne Bosch, Christopher M. Jones, Roger Finlay, Magnus Karlsson, Mats Larsbo, Thomas Keller, Sara Hallin
Summary: Based on a long-term tillage experiment, it was found that non-inversion tillage can increase fungal denitrification activity in the upper soil layers, while reducing tillage intensity can increase the genetic potential for fungal denitrification. Different tillage regimes select for fungal denitrifiers with different functional capabilities and lifestyles, mainly by altering carbon and nitrogen related niches.
SOIL BIOLOGY & BIOCHEMISTRY
(2022)
Editorial Material
Plant Sciences
Johan A. Stenberg, Paul G. Becher, Mattias Jonsson, Magnus Karlsson, Ingvar Sundh, Maria Viketoft
TRENDS IN PLANT SCIENCE
(2023)
Article
Microbiology
Edoardo Piombo, Micol Guaschino, Dan Funck Jensen, Magnus Karlsson, Mukesh Dubey
Summary: This study investigates the composition and activity of fungal secretomes in mycoparasitic and beneficial fungal-plant interactions. The research reveals the importance of secreted proteins in these interactions and identifies potential proteins involved in plant defense and fungal virulence. The findings contribute to our understanding of Clonostachys spp. adaptation and provide a basis for future biocontrol strategies.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Kajsa Himmelstrand, Mikael Brandstrom Durling, Magnus Karlsson, Jan Stenlid, Ake Olson
Summary: This study analyzed 60 mitochondrial genomes of five necrotrophic fungal pathogens on conifers and found low genomic and genetic variation but multiple rearrangements in the complex. Additionally, several shared genes and sequences were discovered. Within-species variation was very low, indicating a slow evolution of the mitochondrial genome in this fungal complex.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Plant Sciences
Carol Kalin, Agnese Kolodinska Brantestam, Anna-Kerstin Arvidsson, Mukesh Dubey, Malin Elfstrand, Magnus Karlsson
Summary: This study evaluated the resistance of six new back-crossed pea breeding lines derived from the cross between the susceptible commercial cultivar Linnea and the landrace PI180693 against Aphanomyces euteiches and Phytophthora pisi. The resistance of these lines was assessed in growth chamber, green house, and field trials. The results showed that the resistance from PI180693 interacted with the virulence levels of A. euteiches and was less effective against root rot caused by P. pisi. However, the breeding lines performed well in commercial production traits, indicating their potential use in commercial breeding programs.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Microbiology
Yvonne Bosch, Grace Pold, Aurelien Saghai, Magnus Karlsson, Christopher M. Jones, Sara Hallin
Summary: Fungal denitrifiers are sparse but cosmopolitan in soils, dominated by saprotrophs and pathogens. Bacterial and archaeal denitrifiers, rather than fungal denitrifiers, are the main contributors to N2O emissions.
MICROBIOLOGY SPECTRUM
(2023)
