Article
Pharmacology & Pharmacy
Yasemin Karaman, Yesim Kaya-Yasar, Cemil Can Eylem, Sevgen Celik Onder, Emirhan Nemutlu, Turgut Emrah Bozkurt, Inci Sahin-Erdemli
Summary: Mitochondrial dysfunction is related to the pathophysiology of airway diseases, and new therapeutic approaches targeting mitochondria are being developed. Hydrogen sulfide (H2S) is involved in the pathophysiological processes of airway inflammation. The effect of mitochondria-targeted slow H2S releasing donor AP39 on LPS-induced airway inflammation in mice was evaluated.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Critical Care Medicine
Chloe Loyer, Arnaud Lapostolle, Tomas Urbina, Alexandre Elabbadi, Jean-Remi Lavillegrand, Thomas Chaigneau, Coraly Simoes, Julien Dessajan, Cyrielle Desnos, Melanie Morin-Brureau, Yannick Chantran, Pierre Aucouturier, Bertrand Guidet, Guillaume Voiriot, Hafid Ait-Oufella, Carole Elbim
Summary: This study reveals that COVID-19 patients have continuously activated neutrophils in their blood, which are associated with endothelial and vascular activation. Additionally, COVID-19 patients have aging-angiogenic neutrophil subsets and reduced oxidative burst response to bacterial stimuli. Moreover, COVID-19 patients who died exhibit more severe neutrophil exhaustion and impaired oxidative burst response.
Article
Biochemistry & Molecular Biology
Israel Perez-Torres, Linaloe Manzano-Pech, Veronica Guarner-Lans, Maria Elena Soto, Vicente Castrejon-Tellez, Ricardo Marquez-Velasco, Alvaro Vargas-Gonzalez, Raul Martinez-Memije, Leonardo Del Valle-Mondragon, Julieta Anabell Diaz-Juarez, Maria Sanchez-Aguilar, Juan Carlos Torres-Narvaez
Summary: Deodorized garlic can reduce heart damage caused by lipopolysaccharide through the cross-talk between the H2S and NO systems.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Jianghong Zhong, Qijing Li, Huqiao Luo, Rikard Holmdahl
Summary: The study shows that mutations in the Ncf1 gene reduce ROS formation, protecting against lung metastasis, with the involvement of IL-1 beta and RAGE in detrimental ROS formation by neutrophils.
COMMUNICATIONS BIOLOGY
(2021)
Article
Plant Sciences
Ji-Ye Lim, Ji-Hyun Lee, Dae-Ho Yun, Young-Mi Lee, Dae-Ki Kim
Summary: Nodakenin exhibits anti-inflammatory, antioxidant, and anti-apoptotic activities by regulating oxidative stress, inflammatory response, and apoptosis-related proteins, suggesting it may be an adjunctive preventive agent for liver injury.
Article
Biochemistry & Molecular Biology
Szilvia Torok, Nikoletta Almasi, Zsuzsanna Valkusz, Aniko Posa, Csaba Varga, Krisztina Kupai
Summary: The study showed that hydrogen sulfide treatment can reduce colonic lesions in experimental rat colitis, decrease the formation of NETs and expression of inflammatory mediators, indicating a potential anti-inflammatory effect of hydrogen sulfide through inhibiting NETs formation, suggesting a new therapeutic approach against IBD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Food Science & Technology
Lucia Recinella, Maria Loreta Libero, Valentina Citi, Annalisa Chiavaroli, Alma Martelli, Roberta Foligni, Cinzia Mannozzi, Alessandra Acquaviva, Simonetta Di Simone, Vincenzo Calderone, Giustino Orlando, Claudio Ferrante, Serena Veschi, Anna Piro, Luigi Menghini, Luigi Brunetti, Sheila Leone
Summary: Multiple studies have shown that aged black garlic has anti-inflammatory, antioxidant, and cardioprotective effects. This study investigated the protective effects of aged black garlic extract (ABGE) alone or in combination with multivitamins on mouse hearts exposed to E. coli lipopolysaccharide. The study also examined the hydrogen sulfide releasing properties and membrane hyperpolarization effect of the formulation composed of ABGE and multivitamins using human aortic smooth muscle cells (HASMCs). The results showed that ABGE, vitamins D and C, and the formulation suppressed the expression of inflammatory genes on mouse hearts and the formulation increased intracellular hydrogen sulfide formation, suggesting its potential use in cardiovascular disease.
Article
Plant Sciences
Beibei Zhang, Mengnan Zeng, Yangyang Wang, Meng Li, Yuanyuan Wu, Ruiqi Xu, Qinqin Zhang, Jufang Jia, Yanjie Huang, Xiaoke Zheng, Weisheng Feng
Summary: OA effectively ameliorated renal function, alleviated inflammatory response and oxidative stress, transformed apoptotic, MAPK and PPAR-gamma signaling pathways in AKI mice, regulated metabolisms of phenylalanine, purine, sphingolipid, taurine, and hypotaurine. Moreover, the role of OA in NRK-52e injury was blocked by FTS.
Article
Biochemistry & Molecular Biology
Lorena Diaz Sanchez, Lissette Sanchez-Aranguren, Keqing Wang, Corinne M. Spickett, Helen R. Griffiths, Irundika H. K. Dias
Summary: Endothelial dysfunction is associated with cardiovascular complications and hydrogen sulfide (H2S) has shown beneficial effects on the vasculature through anti-inflammatory and redox-modulating mechanisms. This study investigates the link between H2S and mitochondrial health in endothelial cells and the mechanisms by which H2S rescues apoptosis. The results demonstrate that TNF-alpha-induced endothelial dysfunction leads to oxidative stress, apoptosis, and mitochondrial alterations, which can be alleviated by post-treatment with the slow-releasing H2S donor, GYY4137.
Article
Pharmacology & Pharmacy
L. Testai, R. Montanaro, L. Flori, E. Pagnotta, V. Vellecco, E. Gorica, L. Ugolini, L. Righetti, V. Brancaleone, M. Bucci, E. Piragine, A. Martelli, L. Di Cesare Mannelli, C. Ghelardini, V. Calderone
Summary: This study investigated the cardioprotective effects of ERU, an isothiocyanate derived from glucosinolate glucoerucin, and explored its mechanism of action, focusing on the role of mitochondrial Kv7.4 potassium channels. ERU was found to release H2S and protect H9c2 cells against oxidative damage. In an in vivo myocardial infarct model, ERU showed protective effects, reducing ischemic area, troponin I levels, and inflammatory outcomes, which were abolished by treatment with a Kv7.4 channel blocker. ERU also exhibited the characteristics of a mitochondrial potassium channel opener in isolated cardiac mitochondria and resulted in persulfidation of mitoKv7.4 channels.
BIOCHEMICAL PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Andre G. Buret, Thibault Allain, Jean-Paul Motta, John L. Wallace
Summary: This article reviews the interaction between hydrogen sulfide (H2S), gut microbiota, and health, highlighting the toxic or therapeutic effects of H2S depending on its concentration and source. Low levels of H2S directly stabilize mucus layers, prevent microbiota biofilm fragmentation, and help resolve inflammation and tissue injury. Additionally, dietary H2S may also modulate the abundance and function of microbiota.
ANTIOXIDANTS & REDOX SIGNALING
(2022)
Article
Biochemistry & Molecular Biology
Hsin-Ying Lu, Hung-Lung Hsu, Chih-Han Li, Shao-Jung Li, Shing-Jong Lin, Chun-Ming Shih, Chun-Che Shih
Summary: Research suggests that H2S may inhibit the formation of AD by reducing inflammatory response, oxidative stress, and positively participating in vascular remodeling. These findings provide evidence for H2S as a novel and promising therapeutic strategy to prevent the development of AD.
Article
Cell Biology
Jianping Sun, Xiaoyu Li, Xiaoyu Gu, Hailong Du, Gengshen Zhang, Jianliang Wu, Feng Wang
Summary: Reports suggest that hydrogen sulfide (H2S) has multiple biological and physiological effects on the pathogenesis of traumatic brain injury (TBI), but the exact molecular mechanism is not fully known. This study showed that H2S treatment alleviated TBI-induced deficits and brain pathology by modulating glutamate-mediated oxidative stress in a p53/GLS-2 pathway-dependent manner.
Article
Immunology
Frances S. Grudzinska, Alice Jasper, Elizabeth Sapey, David R. Thickett, Claudio Mauro, Aaron Scott, Jonathan Barlow
Summary: Neutrophil responses are crucial in inflammatory and infective events, but their dysregulation is linked to poor patient outcomes. Immunometabolism has provided insights into cellular functions in health and disease. This study presents optimized protocols for using XF analysis to probe glycolysis and oxidative burst in neutrophils and highlights the challenges of examining mitochondrial function using this technology. XF technology is a powerful platform with a user-friendly interface, but caution is advised when assessing neutrophil mitochondrial respiration.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Hematology
Carolin Brings, Julia Froebel, Patrick Cadeddu, Ulrich Germing, Rainer Haas, Norbert Gattermann
Summary: This study found that the formation of neutrophil extracellular traps (NETs) was significantly impaired in patients with myelodysplastic syndrome (MDS). MDS neutrophils generated fewer NETs and showed less cellular swelling compared to controls. The decrease in chromatin decondensation and the release of neutrophil elastase were also diminished in MDS patients. These findings suggest a partial myeloperoxidase (MPO) defect may contribute to the deficient NETosis in MDS.
Article
Cell Biology
Kornelia K. Zimmermann, Sashko G. Spassov, Karl M. Strosing, Paul M. Ihle, Helen Engelstaedter, Alexander Hoetzel, Simone Faller
Article
Anesthesiology
Johannes Schmidt, Christin Wenzel, Marlene Mahn, Sashko Spassov, Heidi Cristina Schmitz, Silke Borgmann, Ziwei Lin, Joerg Haberstroh, Stephan Meckel, Sebastian Eiden, Steffen Wirth, Hartmut Buerkle, Stefan Schumann
EUROPEAN JOURNAL OF ANAESTHESIOLOGY
(2018)
Article
Multidisciplinary Sciences
Julie Wagner, Karl M. Strosing, Sashko G. Spassov, Ziwei Lin, Helen Engelstaedter, Sabine Tacke, Alexander Hoetzel, Simone Faller
Article
Cell Biology
Sashko G. Spassov, Christoph Kessler, Rebecca Jost, Stefan Schumann
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2019)
Article
Critical Care Medicine
Johannes Schmidt, Christin Wenzel, Sashko Spassov, Silke Borgmann, Ziwei Lin, Jakob Wollborn, Jonas Weber, Joerg Haberstroh, Stephan Meckel, Sebastian Eiden, Steffen Wirth, Stefan Schumann
CRITICAL CARE MEDICINE
(2020)
Article
Veterinary Sciences
Sara Lozano-Zahonero, Matthias Schneider, Sashko Spassov, Stefan Schumann
LABORATORY ANIMALS
(2020)
Article
Multidisciplinary Sciences
Sashko Spassov, Christin Wenzel, Sara Lozano-Zahonero, Dimona Boycheva, Lea Streicher, Johannes Schmidt, Stefan Schumann
SCIENTIFIC REPORTS
(2020)
Article
Physiology
Christin Wenzel, Johannes Schmidt, Gerald Urban, Stefan Schumann, Sashko Georgiev Spassov
Summary: The study found that spontaneous breathing during surgical intervention under anaesthesia impaired gas exchange and muscle strength in rats, but short-term mechanical ventilation restored blood gas homeostasis and muscle strength. The need to monitor and maintain blood gas balance to ensure quality and relevance of small animal experiments was emphasized.
EXPERIMENTAL PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Sashko G. Spassov, Simone Faller, Andreas Goeft, Marc-Nicolas A. Von Itter, Andreas Birkigt, Peter Meyerhoefer, Andreas Ihle, Raphael Seiler, Stefan Schumann, Alexander Hoetzel
Summary: This study investigates the anti-inflammatory effects of hydrogen sulfide (H2S) on lung cells subjected to mechanical stretch. The results demonstrate that H2S can reduce inflammation in macrophages, preserve endothelial integrity, and limit inflammatory response in neutrophils by maintaining redox homeostasis.
Article
Biophysics
Silke Borgmann, Kim Linz, Christian Braun, Patryk Dzierzawski, Sashko Spassov, Christin Wenzel, Stefan Schumann
Summary: In this study, two novel methods for estimating the lung area using functional tidal images or active contouring methods were presented. A convolutional neural network was trained to determine whether or not the heart region was visible within tidal images. The results showed that the method based on functional tidal images provided the best estimate of the lung area.
PHYSIOLOGICAL MEASUREMENT
(2022)
Article
Cell Biology
Ulrich Goebel, Stefanie Scheid, Sashko Spassov, Nils Schallner, Jakob Wollborn, Hartmut Buerkle, Felix Ulbrich
Summary: Experimental studies have shown that argon can regulate neuronal damage and inflammation by inhibiting certain transcription factors, thereby exerting neuroprotective effects. Further research indicates that argon can reduce the inflammatory response after retinal ischemia/reperfusion injury.
NEURAL REGENERATION RESEARCH
(2021)