Article
Multidisciplinary Sciences
Aaron A. Smargon, Assael A. Madrigal, Brian A. Yee, Kevin D. Dong, Jasmine R. Mueller, Gene W. Yeo
Summary: This study reveals that Cas9 can interact with endogenous human RNA transcripts in human cells, and this interaction is correlated with elevated RNA editing even under guide RNA co-expression. However, there is no evidence to support the hypothesis that human RNAs can guide Cas9 to bind and cleave human genomic DNA broadly.
NATURE COMMUNICATIONS
(2022)
Article
Engineering, Biomedical
Jun Suzuka, Masumi Tsuda, Lei Wang, Shinji Kohsaka, Karin Kishida, Shingo Semba, Hirokazu Sugino, Sachiyo Aburatani, Martin Frauenlob, Takayuki Kurokawa, Shinya Kojima, Toshihide Ueno, Yoshihiro Ohmiya, Hiroyuki Mano, Kazunori Yasuda, Jian Ping Gong, Shinya Tanaka
Summary: The study reveals that a double-network hydrogel can rapidly reprogramme differentiated tumor cells into cancer stem cells by inducing phosphorylation of tyrosine kinases. After culturing human brain cancer cells on the double-network gel, they exhibited higher tumorigenicity when intracranially injected in immunodeficient mice compared to cells cultured on single-network gels.
NATURE BIOMEDICAL ENGINEERING
(2021)
Article
Multidisciplinary Sciences
Rasool Erfani, Cameron Carmichael, Thea Sofokleous, Qiuyu Wang
Summary: Plasma therapy is an exciting and novel cancer treatment method that can be influenced by treatment time and different parameters. Changing the polarity of the reactor can alter the plasma discharge regime, thereby directly affecting the treatment effectiveness. This treatment method can induce cell death, which is not completely counteracted by N-acetyl cysteine. Gene expression analysis also identified key genes associated with plasma-induced cell death.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Matthias Brandt, Volker Gerke, Timo Betz
Summary: Endothelial cells play a crucial role in inflammation, and the pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) has a significant mechanical impact on these cells within a short time.
Article
Engineering, Biomedical
Amro A. A. Hussien, Barbara Niederoest, Maja Bollhalder, Nils Goedecke, Jess G. G. Snedeker
Summary: Extracellular matrix stiffness plays a crucial role in regulating cellular states. By developing a biomaterial composed of mechanovariant silicone substrates, this study investigates the mechanosensing responses of human tendon-derived stromal cells. The results show that matrix elasticity affects transcriptional programs related to chromatin remodeling, Hippo signaling, cell stemness, synapse assembly, and angiogenesis. The findings highlight the importance of matrix biophysical cues in determining the transcriptional identity of tendon cells and regulating mechanosensitive processes.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Microbiology
Sara Nunes, Rafael Tiburcio, Icaro Bonyek-Silva, Pablo Rafael Oliveira, Ricardo Khouri, Viviane Boaventura, Aldina Barral, Claudia Brodskyn, Natalia Machado Tavares
Summary: Skin ulcers in cutaneous leishmaniasis (CL) are influenced by the interaction between dendritic cells (DCs) and natural killer (NK) cells. This study identified the crosstalk between DCs and NK cells pathway as a potential pathway involved in the pathogenesis of CL, with molecules such as TLR4, TNFRSF1B, IL-15, IL-6, CD40, CCR7, TNF, and IFNG playing significant roles. The expression of CCR7, in particular, was found to be correlated with lesion development.
Article
Physics, Particles & Fields
Dan Wen, Yongshun Hu
Summary: In the framework of linearized quantum gravity, we studied the quantum gravitational quadrupole-quadrupole interaction between two entangled non point-like objects in the presence of both Dirichlet and Neumann boundaries. The interaction can be enhanced or weakened depending on the geometrical arrangement of the objects with respect to the boundaries. The results showed different behaviors in the near and far regimes, with a new r(-2) far-regime behavior observed in the perpendicular configuration.
EUROPEAN PHYSICAL JOURNAL C
(2023)
Article
Multidisciplinary Sciences
Paula Ordonez, Kate N. Bishop, Jonathan P. Stoye, Harriet Cordelia Theed Groom
Summary: SAMHD1 inhibits HIV-1 replication in quiescent myeloid cells. A two-color restriction assay has been developed to analyze SAMHD1 in U937 cells, demonstrating a significant reduction in HIV infection. Flow cytometry and viruses encoding mutated proteins have been utilized to further understand the restriction activity of SAMHD1.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Multidisciplinary Sciences
Yusuke Kuwahara, Kazutaka Yasukawa, Koichiro Fujinaga, Tatsuo Nozaki, Junichiro Ohta, Honami Sato, Jun-Ichi Kimura, Kentaro Nakamura, Yusuke Yokoyama, Yasuhiro Kato
Summary: The solid earth responds to climate change through chemical weathering and seafloor magmatism, causing short-term climate fluctuations during glacial-interglacial cycles. This suggests that climatic changes on shorter timescales can trigger rapid feedbacks from the solid earth.
SCIENTIFIC REPORTS
(2021)
Article
Oncology
Zheng Wang, Chengyan Chai, Rui Wang, Yimei Feng, Lei Huang, Yiming Zhang, Xia Xiao, Shijie Yang, Yunfang Zhang, Xi Zhang
Summary: This study utilized advanced single-cell RNA sequencing to construct a comprehensive atlas of over 130,000 single-MSC transcriptomes across multiple tissues and donors, revealing seven tissue-specific and five conserved MSC subpopulations with distinct gene expression signatures. The study demonstrated the significant contribution of extracellular matrix to MSC heterogeneity and identified potential improvements in MSC-based therapeutic efficacy.
CLINICAL AND TRANSLATIONAL MEDICINE
(2021)
Article
Pharmacology & Pharmacy
Madhu Prakash Ganeshkar, Anjana Thatesh Gaddigal, Parashuram Shivappa, Paramanna Bhagappa Poojari, Premakshi Hucharayappa Goder, Chandrappa Mukappa Kamanavalli
Summary: Nanomaterials are being extensively researched in nanomedicine, but their use is limited by the lack of comprehensive toxicity profiles. In this study, a novel bioconjugate nanoparticle, Rut-PEG-CeO2NPs, was fabricated and evaluated for its antibacterial, hemolytic, anticoagulation, anti-angiogenesis, and cytotoxicity properties. The results showed that Rut-PEG-CeO2NPs exhibited broad spectrum antibacterial activity and caused significant cell damage. It also induced higher hemolysis in diabetic donors compared to healthy donors. Moreover, Rut-PEG-CeO2NPs showed anticoagulation and anti-angiogenic effects, as well as higher cell viability in leukemia cells compared to CeO2NPs and Rutin alone. These findings suggest that Rut-PEG-CeO2NPs have potential therapeutic applications due to their unique properties.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Genetics & Heredity
Yong-Yu Huang, Guo-Dong Wang, Jun-Sheng Liu, Li-Li Zhang, Shi-Yu Huang, Yi-Lei Wang, Zhang-Wu Yang, Hui Ge
Summary: RNA-seq analysis of Penaeus vannamei revealed that the growth differences between individuals are associated with biological processes such as sensory perception, response to stimuli, and response to stress. Key differentially expressed genes include opsins, heat shock proteins, and superoxide dismutase.
Article
Genetics & Heredity
Francesca Antonaros, Rossella Zenatelli, Giulia Guerri, Matteo Bertelli, Chiara Locatelli, Beatrice Vione, Francesca Catapano, Alice Gori, Lorenza Vitale, Maria Chiara Pelleri, Giuseppe Ramacieri, Guido Cocchi, Pierluigi Strippoli, Maria Caracausi, Allison Piovesan
Summary: The study analyzed gene expression profiles of T21 and normal control blood cells, finding that the most over-expressed genes encode interferon-induced proteins and genes involved in mitochondrial translation and energy production were also altered.
Article
Cell Biology
Juergen Fritsch, Julia Frankenheim, Lothar Marischen, Timea Vadasz, Anja Troeger, Stefan Rose-John, Dirk Schmidt-Arras, Wulf Schneider-Brachert
Summary: ADAM17 plays a key role in fine-tuning death receptor signaling, and its absence can increase cell death. A better understanding of TNF-R1 superfamily receptor decisions may lead to more effective treatments for infectious, inflammatory diseases, or cancer.
Letter
Cell & Tissue Engineering
Shaoyang Zhang, Jing Yi Wang, Baojie Li, Feng Yin, Huijuan Liu
Summary: UC-MSCs exhibit heterogeneity during in vitro expansion, but single-cell RNA sequencing has identified two distinct populations of freshly isolated UC-MSCs. These populations show different gene expression patterns, suggesting that they may be suitable for treating different diseases.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell Biology
Svantje Tauber, Swantje Hauschild, Katrin Paulsen, Annett Gutewort, Christiane Raig, Eva Huerlimann, Josefine Biskup, Claudia Philpot, Hartwin Lier, Frank Engelmann, Antonella Pantaleo, Augusto Cogoli, Proto Pippia, Liliana E. Layer, Cora S. Thiel, Oliver Ullrich
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
(2015)
Article
Biotechnology & Applied Microbiology
Cora S. Thiel, Swantje Hauschild, Svantje Tauber, Katrin Paulsen, Christiane Raig, Arnold Raem, Josefine Biskup, Annett Gutewort, Eva Huerlimann, Felix Unverdorben, Isabell Buttron, Beatrice Lauber, Claudia Philpot, Hartwin Lier, Frank Engelmann, Liliana E. Layer, Oliver Ullrich
BIOMED RESEARCH INTERNATIONAL
(2015)
Article
Biotechnology & Applied Microbiology
Katrin Paulsen, Svantje Tauber, Claudia Dumrese, Gesine Bradacs, Dana M. Simmet, Nadine Goelz, Swantje Hauschild, Christiane Raig, Stephanie Engeli, Annett Gutewort, Eva Huerlimann, Josefine Biskup, Felix Unverdorben, Gabriela Rieder, Daniel Hofmaenner, Lisa Mutschler, Sonja Krammer, Isabell Buttron, Claudia Philpot, Andreas Huge, Hartwin Lier, Ines Barz, Frank Engelmann, Liliana E. Layer, Cora S. Thiel, Oliver Ullrich
BIOMED RESEARCH INTERNATIONAL
(2015)
Article
Multidisciplinary Sciences
Svantje Tauber, Beatrice A. Lauber, Katrin Paulsen, Liliana E. Layer, Martin Lehmann, Swantje Hauschild, Naomi R. Shepherd, Jennifer Polzer, Juergen Segerer, Cora S. Thiel, Oliver Ullrich
Article
Multidisciplinary Sciences
Cora S. Thiel, Andreas Huge, Swantje Hauschild, Svantje Tauber, Beatrice A. Lauber, Jennifer Polzer, Katrin Paulsen, Hartwin Lier, Frank Engelmann, Burkhard Schmitz, Andreas Schuette, Liliana E. Layer, Oliver Ullrich
Article
Cell Biology
Svantje Tauber, Swantje Hauschild, Claudia Crescio, Christian Secchi, Katrin Paulsen, Antonella Pantaleo, Angela Saba, Isabell Buttron, Cora Sandra Thiel, Augusto Cogoli, Proto Pippia, Oliver Ullrich
CELL COMMUNICATION AND SIGNALING
(2013)
Article
Biochemistry & Molecular Biology
Svantje Tauber, Swantje Christoffel, Cora Sandra Thiel, Oliver Ullrich
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Article
Multidisciplinary Sciences
Cora S. Thiel, Diane de Zelicourt, Svantje Tauber, Astrid Adrian, Markus Franz, Dana M. Simmet, Kathrin Schoppmann, Swantje Hauschild, Sonja Krammer, Miriam Christen, Gesine Bradacs, Katrin Paulsen, Susanne A. Wolf, Markus Braun, Jason Hatton, Vartan Kurtcuoglu, Stefanie Franke, Samuel Tanner, Samantha Cristoforetti, Beate Sick, Bertold Hock, Oliver Ullrich
SCIENTIFIC REPORTS
(2017)
Article
Multidisciplinary Sciences
Cora S. Thiel, Swantje Hauschild, Andreas Huge, Svantje Tauber, Beatrice A. Lauber, Jennifer Polzer, Katrin Paulsen, Hartwin Lier, Frank Engelmann, Burkhard Schmitz, Andreas Schuette, Liliana E. Layer, Oliver Ullrich
SCIENTIFIC REPORTS
(2017)
Article
Biochemistry & Molecular Biology
Cora Sandra Thiel, Swantje Christoffel, Svantje Tauber, Christian Vahlensieck, Diane de Zelicourt, Liliana E. Layer, Beatrice Lauber, Jennifer Polzer, Oliver Ullrich
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)