Article
Plant Sciences
Rui Lin, Jianing Song, Mingjia Tang, Lingyu Wang, Jingquan Yu, Yanhong Zhou
Summary: This study reveals that CaM6 negatively regulates plant cold tolerance by attenuating ICE1 and decreasing the expression levels of ICE1-dependent stress- and metabolism-related genes. The findings shed light on the mechanism of plant response to cold stress and provide insight into the role of CaM6 in the regulation of ICE1.
Article
Biochemistry & Molecular Biology
Zhuoyang Li, Diyi Fu, Xi Wang, Rong Zeng, Xuan Zhang, Jinge Tian, Shuaisong Zhang, Xiaohong Yang, Feng Tian, Jinsheng Lai, Yiting Shi, Shuhua Yang
Summary: This study identifies a negative regulator, bZIP68, that negatively regulates cold tolerance in maize and reveals its differential expression in response to cold stress between maize and its wild ancestor teosinte, which is attributed to natural variation in the bZIP68 promoter.
Article
Plant Sciences
Dongmei Zhang, Kaijie Zhu, Xingyi Shen, Jian Meng, Xue Huang, Yuqi Tan, Francesca Cardinale, Jihong Liu, Guohuai Li, Junwei Liu
Summary: Two ethylene response factors (ERFs), PpERF98 and PpERF1, have been identified as negative regulators in peach response to L. theobromae infection. Silencing of PpERF98-1/2 increases peach resistance to L. theobromae through increasing SA content and pathogenesis-related gene expression. PpERF98-1/2 directly bind the promoters of PpERF1 genes and activate the ERF branch of the jasmonate/ethylene signaling pathway.
Article
Plant Sciences
Tian Ma, Shuge Wang, Cunyi Sun, Jiawang Tian, Hong Guo, Sujuan Cui, Hongtao Zhao
Summary: In this study, the mutation of LFR in Arabidopsis resulted in hypersensitivity to freezing stress and reduced expression levels of CBFs and COR genes. LFR was found to interact directly with ICE1 and activate ICE1 and CBF3 gene expression in response to cold stress.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Changxuan Xia, Yanshan Gong, Kang Chong, Yunyuan Xu
Summary: The study showed that OsPP2C27 negatively regulates the OsMAPK3-OsbHLH002-OsTPP1 signaling pathway by directly dephosphorylating both OsMAPK3 and OsbHLH002, preventing the sustained activation of the positive pathway for cold stress and maintaining normal growth under chilling conditions.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Plant Sciences
Jian-Ping An, Rui-Rui Xu, Xin Liu, Ling Su, Kuo Yang, Xiao-Fei Wang, Gui-Luan Wang, Chun-Xiang You
Summary: In this study, it was demonstrated that the AP2/ERF family protein MdABI4 integrates jasmonic acid and abscisic acid signals to modulate cold tolerance through the JAZ-ABI4-ICE1-CBF regulatory cascade in apple. Furthermore, the negative modulation of MdABI4-improved cold tolerance by MdJAZ1 and MdJAZ2 was found to be through interference with the interaction between MdABI4 and MdICE1 proteins. These findings provide insights into the crosstalk between jasmonic acid and abscisic acid signals in response to cold stress.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Meng Li, Xiaoyu Duan, Ge Gao, Tao Liu, Hongyan Qi
Summary: This study revealed that arginine decarboxylase (ADC) functions as a positive regulator of melon seedling cold tolerance, with the transcription factors CmABF1 and CmCBF4 directly targeting ADC to trigger its expression. Additionally, other CBF and ABF members were also found to have functional redundancy and complementarity with CmABF1 and CmCBF4.
HORTICULTURE RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Jianyan Huang, Xiaobo Zhao, Marco Burger, Yurong Wang, Joanne Chory
Summary: The study revealed the positive role of ethylene signaling, ERF95, and ERF97 in basal thermotolerance of Arabidopsis thaliana and established the relationship between them. EIN3 physically binds to the promoters of ERF95 and ERF97, enhancing plant response to heat stress. ERF95 and ERF97 function genetically downstream of EIN3 and increase basal thermotolerance by regulating a common set of target genes.
Article
Plant Sciences
Chunbo Song, Mengbo Wu, Ying Zhou, Zehao Gong, Weiwei Yu, Yi Zhang, Zhenfeng Yang
Summary: The research identified two cold-responsive NAC transcription factors in bananas that negatively regulate cold tolerance in banana fruits by upregulating the expression of phospholipid degradation genes, forming a positive feedback loop to induce phospholipid degradation and produce phosphatidic acid. Ethylene was found to directly inhibit phospholipid degradation in banana and transgenic tomato fruits.
HORTICULTURE RESEARCH
(2022)
Article
Plant Sciences
Hyung-Woo Jeon, Mary E. Byrne
Summary: Plant leaves have various shapes due to different species, developmental stages, and environments. Mutations in the homeodomain transcription factors Saw1 and Saw2 can lead to earlier appearance of leaf margin serrations.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Yexing Jing, Ziyi Yang, Ruizhen Yang, Yunwei Zhang, Weihua Qiao, Yun Zhou, Jiaqiang Sun
Summary: Drought stress causes significant crop losses annually, jeopardizing global food security. Identification of genetic components related to drought tolerance in plants is crucial. This study reveals that loss of function of the chromatin remodeling factor PICKLE (PKL) enhances drought tolerance in Arabidopsis.
Review
Plant Sciences
Siti Nor Akmar Abdullah, Azzreena Mohamad Azzeme, Kobra Yousefi
Summary: This paper reviews the mechanisms of plant response to cold stress, including transcription factor regulation, early and late responses, and long-term memory reprogramming. The regulatory network can be divided into CBF-dependent and independent pathways. In addition, post-transcriptional regulation by miRNAs, control during ribosomal translation, and post-translational regulation have important effects on the cellular abundance of key regulatory factors.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Mingzhe Sun, Yang Shen, Yue Chen, Yan Wang, Xiaoxi Cai, Junkai Yang, Bowei Jia, Weifeng Dong, Xi Chen, Xiaoli Sun
Summary: miR1320, a rice species-specific microRNA, regulates cold stress tolerance in rice by targeting the ethylene-responsive transcription factor OsERF096 and repressing the JA-mediated cold signaling pathway. The study provides insights into the regulatory mechanism of miR1320 and OsERF096 under cold stress and demonstrates the impact of OsERF096 on hormone content and signaling pathways.
Article
Plant Sciences
Pengpeng Zheng, Lei Cao, Cheng Zhang, Xue Fang, Lihuan Wang, Min Miao, Xiaofeng Tang, Yongsheng Liu, Shuqing Cao
Summary: Previous studies have shown the post-translational modifications and protein stability regulation of ICE1 in cold stress, but the interaction of ICE1 with other transcription factors and their role in cold stress tolerance and transition to growth are not well understood. In this study, we found that MBP43 directly binds to the promoters of CBF genes to repress their expression, negatively regulating freezing tolerance. Furthermore, PRL1 promotes MYB43 protein degradation to attenuate its repression of CBF genes and antagonism with ICE1, thereby modulating plant cold stress responses.
Article
Biochemistry & Molecular Biology
Ozgur Oksuz, Jonathan E. Henninger, Robert Warneford-Thomson, Ming M. Zheng, Hailey Erb, Adrienne Vancura, Kalon J. Overholt, Susana Wilson Hawken, Salman F. Banani, Richard Lauman, Lauren N. Reich, Anne L. Robertson, Nancy M. Hannett, Tong I. Lee, Leonard I. Zon, Roberto Bonasio, Richard A. Young
Summary: Transcription factors (TFs) regulate gene expression programs through two domains, one that binds specific DNA sequences and another that binds protein coactivators or corepressors. It has been discovered that at least half of TFs can also bind RNA through a previously unrecognized domain similar to the arginine-rich motif of the HIV transcriptional activator Tat. RNA binding enhances TF function by promoting dynamic interactions between DNA, RNA, and TF on chromatin. TF-RNA interactions are conserved and crucial for vertebrate development, but can be disrupted in diseases. The ability to bind DNA, RNA, and protein is a fundamental property of many TFs and essential for their gene regulatory function.
Review
Biochemistry & Molecular Biology
Leia Colin, Felix Ruhnow, Jian-Kang Zhu, Chunzhao Zhao, Yang Zhao, Staffan Persson
Summary: Salt stress causes ionic toxicity, osmotic stress, and oxidative stress, directly affecting plant growth and development. Plants perceive salt stress through the cell wall and coordinate effective responses. Some mechanisms underlying these processes have been elucidated.
Review
Agronomy
Takuya Suzaki
Summary: During plant evolution, leguminous and a few other plant species have developed root nodule symbiosis (RNS), a nitrogen nutrient acquisition strategy based on mutual interaction with nitrogen-fixing bacteria. Recent studies have focused on understanding the molecular mechanisms underlying nodule organogenesis, with particular emphasis on the NODULE INCEPTION transcription factor and its target genes. Additionally, the role of the legume SHORTROOTSCARECROW module in the unique properties of legume cortical cells has been elucidated.
Article
Biochemistry & Molecular Biology
Hanna Nishida, Takuya Suzaki
Summary: Root nodule symbiosis is influenced by nitrogen availability and Lotus japonicus NODULE INCEPTION (NIN)-LIKE PROTEIN (NLP) transcription factors LjNLP1 and LjNLP4 play important roles in gene regulation and nodulation response to nitrate. LjNLP1 specifically regulates the expression of the NITRATE TRANSPORTER 2 (LjNRT2) gene family, while both LjNLP1 and LjNLP4 regulate the expression of the LjNRT1 gene family, suggesting distinct functions in nitrate transport regulation.
GENES & GENETIC SYSTEMS
(2023)
Article
Biochemistry & Molecular Biology
Islam M. Y. Abdellatif, Shaoze Yuan, Shizue Yoshihara, Takuya Suzaki, Hiroshi Ezura, Kenji Miura
Summary: The study finds that PHY photoreceptors play an important role in the drought response of tomato plants. The mutant lines of PHYA and PHYB in tomatoes show enhanced drought tolerance through reduced membrane damage, increased expression of ROS scavenger genes, inhibition of H2O2 accumulation, and enhanced antioxidant activities. These mutants also exhibit higher expression levels of aquaporins and higher relative water content, indicating improved hydration status under drought stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Mingjing Wang, Hailong Zhang, Xiaonan Zhao, Jingwen Zhou, Guochen Qin, Yuqi Liu, Xiaoyue Kou, Zhenjie Zhao, Tao Wu, Jian-Kang Zhu, Xianzhong Feng, Lixin Li
Summary: The gene AtSYP81 in Arabidopsis thaliana plays a critical role in root growth and development by regulating peroxisome- and peroxidase-mediated ROS homeostasis. Knockdown of AtSYP81 resulted in reduced root meristem activity and disruption of root stem cell niche identity. Transcriptome analysis revealed changed expression of class III peroxidases, adding evidence of the regulatory role of AtSYP81 in ROS signaling.
Article
Plant Sciences
Sheng Wu, Anqi Zhou, Kozue Hiugano, Akiyoshi Yoda, Xiaonan Xie, Kenji Yamane, Kenji Miura, Takahito Nomura, Yanran Li
Summary: We identified a strigol synthase (cytochrome P450 711A enzyme) in the Prunus genus through gene screening and confirmed its unique catalytic activity. We reconstructed the biosynthetic pathway of strigol in Nicotiana benthamiana and reported the total biosynthesis of strigol from xylose in the Escherichia coli-yeast consortium, enabling large-scale production.
Article
Plant Sciences
Akiyoshi Yoda, Xiaonan Xie, Kaori Yoneyama, Kenji Miura, Christopher S. P. McErlean, Takahito Nomura
Summary: Seeds of root parasitic plants are induced to germinate by strigolactones exudated from host roots. The biosynthesis pathway of 5-deoxystrigol, a major strigolactone, has not been fully understood. In this study, researchers identified Sobic.005G213500 as a regulator that affects the stereoselective biosynthesis of 5-deoxystrigol and provides insights into how different strigolactones are produced to combat parasitic weed infestations.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Plant Sciences
Sayuri Ando, Mika Nomoto, Hidekazu Iwakawa, Simon Vial-Pradel, Lilan Luo, Michiko Sasabe, Iwai Ohbayashi, Kotaro T. Yamamoto, Yasuomi Tada, Munetaka Sugiyama, Yasunori Machida, Shoko Kojima, Chiyoko Machida
Summary: Arabidopsis ASYMMETRIC LEAVES2 (AS2) is crucial for the formation of flat symmetric leaves. AS2 represses the expression of ETTIN/AUXIN RESPONSE FACTOR3 (ETT/ARF3) gene. The formation of AS2 bodies is tightly linked to the morphology and functions of the nucleolus.
Article
Biodiversity Conservation
Takatoshi Wakabayashi, Megumi Nakayama, Yurie Kitano, Masato Homma, Kenji Miura, Hirosato Takikawa, Masaharu Mizutani, Yukihiro Sugimoto
Summary: Parasitic witchweeds (Striga species) are a serious threat to food security in Africa, attacking cereal grains and legumes. The chemicals released from host roots, known as germination stimulants, mainly strigolactones (SLs), were first identified in cotton root exudates over 50 years ago. This discovery of a complete biosynthesis pathway for strigolactones in cotton has the potential to enhance our understanding of SL-mediated rhizosphere interactions and improve cotton's effectiveness as a trap crop.
PLANTS PEOPLE PLANET
(2023)
Article
Biochemistry & Molecular Biology
Masahide Seki, Yuta Kuze, Xiang Zhang, Ken-ichi Kurotani, Michitaka Notaguchi, Haruki Nishio, Hiroshi Kudoh, Takuya Suzaki, Satoko Yoshida, Sumio Sugano, Tomonao Matsushita, Yutaka Suzuki
Summary: TSS-seq2 is an improved method for detecting TSS, with higher accuracy and less bias compared to previous methods, achieved by introducing split ligation and other modifications. It can be conducted with as little as 5 ng of RNA and has been successfully applied to TSS analysis of four plant species.
NUCLEIC ACIDS RESEARCH
(2023)
Review
Plant Sciences
Songkui Cui, Shoko Inaba, Takuya Suzaki, Satoko Yoshida
Summary: Plants have evolved different strategies to meet their nutritional needs, with parasitic plants using specialized structures to invade host plants and obtain nutrients, and legumes developing nodules to fix nitrogen. The formation of these organs is controlled by plant hormones and environmental factors, demonstrating the plasticity of plant organ development.
CURRENT OPINION IN PLANT BIOLOGY
(2023)
Article
Plant Sciences
Shohei Nosaki, Nobutaka Mitsuda, Shingo Sakamoto, Kazuki Kusubayashi, Ayumi Yamagami, Yuqun Xu, Thi Bao Chau Bui, Tohru Terada, Kenji Miura, Takeshi Nakano, Masaru Tanokura, Takuya Miyakawa
Summary: The transcription factor BIL1/BZR1 is involved in regulating gene expression in response to brassinosteroid hormones in plants. This study reveals that BIL1/BZR1 can repress the transcription of certain genes by binding tightly to specific regions on the DNA. Furthermore, the study demonstrates that BIL1/BZR1 achieves selectivity for particular DNA sequences by reading the shape of the DNA rather than directly recognizing the nucleobases. These findings provide valuable insights into the molecular and structural basis of transcriptional repression by BIL1/BZR1 and its role in gene regulation.
Review
Biochemistry & Molecular Biology
Xiaohan Li, Siyan Xu, Martina Bianca Fuhrmann-Aoyagi, Shaoze Yuan, Takeru Iwama, Misaki Kobayashi, Kenji Miura
Summary: Global warming and climate change have had a significant impact on plant growth and food production. To ensure sustainable crop yields, it is crucial to minimize these effects. The use of genome-editing technologies, such as CRISPR/Cas9 and TALENs, has recently gained attention in plant breeding for genetic modification. These technologies have the potential to enhance our understanding of gene function and improve crops' tolerance to abiotic stresses.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)
Article
Plant Sciences
Qingchao Chen, Tao Hu, Xiaohua Li, Chun-Peng Song, Jian-Kang Zhu, Liqing Chen, Yang Zhao
Summary: During drought stress, plants increase their root:shoot ratio by enhancing the long-distance sucrose transport, mediated by the phosphorylation of sucrose transporters SWEET11 and 12. This phosphorylation, induced by drought and abscisic acid treatments, improves sugar loading in the phloem and supports enhanced root growth.
