Article
Biochemistry & Molecular Biology
Aleksandra Rodak, Katharina Stadlbauer, Madhusudhan Reddy Bobbili, Oskar Smrzka, Florian Rueker, Gordana Wozniak Knopp
Summary: This study developed an antibody-drug conjugate that showed cytotoxicity for IgE-positive cells. The antibody had fast internalization and strict target specificity, providing a valuable starting point for the development of an anti-IgE cell-depleting agent.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Pharmacology & Pharmacy
Anna Olewicz-Gawlik, Arleta Kowala-Piaskowska
Summary: Growing evidence suggests the pathogenic role of autoreactive IgE in autoimmune diseases. The incidence of autoimmune and allergic diseases in industrialized countries is consistently increasing, leading to efforts to understand the regulation of IgE-mediated mechanisms. Autoantibodies of IgE have been found in patients with autoimmune diseases, and it is now known that self-reactive IgE can cause inflammatory response in several conditions. Understanding these mechanisms has led to the successful use of anti-IgE strategies in managing these disorders. This review discusses the current knowledge on IgE-mediated autoimmunity and anti-IgE treatment, and paves the way for further exploration.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Medicine, General & Internal
Cesar Picado, Inaki Ortiz de Landazuri, Alexandru Vlagea, Irina Bobolea, Ebymar Arismendi, Rosanel Amaro, Jacobo Sellares, Joan Bartra, Raimon Sanmarti, Jose Hernandez-Rodriguez, Jose-Manuel Mascaro, Jordi Colmenero, Eva C. Vaquero, Mariona Pascal
Summary: This study highlights the clinical manifestations of Selective IgE deficiency (SIgED), which include various infectious diseases, autoimmune conditions, eczematous dermatitis, and malignancies. Patients with SIgED have an increased risk of developing malignancies, warranting further prospective studies to better characterize this condition and determine its inclusion in the list of antibody deficiencies.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Hematology
Peter A. A. Norris, Gurleen Kaur, Ramsha Khan, Guangheng Zhu, Heyu Ni, Alan H. Lazarus
Summary: Anti-CD44 exhibits anti-inflammatory effects in murine ITP by inhibiting macrophage phagocytosis through blockade of Fc gamma R, potentially serving as an alternative to IVIg. The specific Fc gamma R blockade mechanism of anti-CD44 contributes to its therapeutic efficacy in ITP models, highlighting its potential as a treatment option.
Article
Food Science & Technology
Andy Shing Seng Lim, Kae Yi Tan, Naeem H. Quraishi, Saud Farooque, Zahoor Ahmed Khoso, Kavi Ratanabanangkoon, Choo Hock Tan
Summary: Snakebite envenoming is a neglected tropical disease in South Asia. The study evaluated the Pakistani Viper Antivenom (PVAV) raised against vipers of Pakistani origin. PVAV showed high purity, immuno-specificity and neutralization efficacy against the venoms of the specific vipers. It has the potential to be a new domestic antivenom for viperid envenoming in Pakistan.
Article
Medical Laboratory Technology
Jian-Hang Xue, Yong-Jing Wang, Wei Li, Qiu-Ling Li, Qiu-Yan Xu, Jian-Jun Niu, Li-Li Liu
Summary: There is a strong correlation between neutralizing antibodies and anti-receptor-binding domain (RBD) IgG antibodies, suggesting that anti-RBD IgG antibody levels can accurately assess immunity following SARS-CoV-2 infection or vaccination.
ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE
(2022)
Article
Medicine, General & Internal
Cesar Picado, Adriana P. Garcia-Herrera, Jose Hernandez-Rodriguez, Alexandru Vlagea, Mariona Pascal, Joan Bartra, Jose Manuel Mascaro
Summary: Selective immunoglobulin E deficiency (SIgED) is an unrecognized primary immunodeficiency that is associated with various skin manifestations and autoimmune diseases.
JOURNAL OF CLINICAL MEDICINE
(2022)
Review
Immunology
Zicheng Liu, Daria Trifonova, Inna Tulaeva, Ksenja Riabova, Antonina Karsonova, Evgeny Kozlov, Olga Elisyutina, Musa Khaitov, Margarete Focke-Tejkl, Ting-Huan Chen, Alexander Karaulov, Rudolf Valenta
Summary: Albumins from animals are highly cross-reactive allergens for patients suffering from IgE-mediated allergy. The mechanisms of albumin sensitization have not yet been investigated in detail, but it is important for the development of allergen-specific prevention and immunotherapy strategies.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Organic
Ravindra S. Phatake, Tizian Mueller, Arthur Averdunk, Urs Gellrich
Summary: We have discovered a bis(pentafluorophenyl)borane-catalyzed isomerization reaction that selectively converts terminal alkenes to internal alkenes. This transformation can be achieved under mild reaction conditions and with low catalyst loadings. Interestingly, our method also allows for long-range isomerization of aryl alkenes. We have investigated the reaction mechanism using dispersion-corrected spin-component scaled double hybrid DFT computations, revealing a hydroboration/retro-hydroboration sequence.
ORGANIC CHEMISTRY FRONTIERS
(2023)
Article
Biology
Kangcheng Song, Miao Wei, Wenjun Guo, Li Quan, Yunlu Kang, Jing-Xiang Wu, Lei Chen
Summary: TRPC5 channel is a nonselective cation channel involved in various physiological processes. Inhibitors of TRPC5, such as clemizole and HC-070, stabilize the ion channel in a nonconductive closed state, potentially offering new therapeutic strategies for anxiety disorders, depression, and kidney diseases. The cryo-EM structures of human TRPC5 with inhibitors provide insights into the binding sites and inhibitory mechanisms, facilitating the development of more effective inhibitors targeting TRPC5.
Review
Allergy
Oscar Palomares, Dirk Elewaut, Peter M. Irving, Xavier Jaumont, Paolo Tassinari
Summary: Autoimmune diseases have a high prevalence rate, but effective and targeted treatments are still lacking. Regulatory T cells play an important role in many autoimmune diseases, and immunoglobulin E also appears to be involved in autoimmune diseases. Anti-IgE treatment may restore the balance of regulatory T cells and could be used in the treatment of autoimmune diseases involving regulatory T cells.
Article
Multidisciplinary Sciences
Shabareesh Pidathala, Aditya Kumar Mallela, Deepthi Joseph, Aravind Penmatsa
Summary: The researchers used the Drosophila melanogaster dopamine transporter as a surrogate to study the structure and function of norepinephrine, revealing potential paradigms for designing chronic pain inhibitors.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Yannic C. Bartsch, Deniz Cizmeci, Jaewon Kang, Hailong Gao, Wei Shi, Abishek Chandrashekar, Ai-ris Y. Collier, Bing Chen, Dan H. Barouch, Galit Alter
Summary: The omicron (B.1.1.529) BA.1 variant has mutations that evade vaccine immunity, but severe disease is still relatively low due to T cell recognition and non-neutralizing antibodies. However, the BA.2 sublineage, which is more transmissible, may evade some vaccine-induced responses. This study found that vaccine-induced immune responses were compromised against both omicron sublineages, but non-neutralizing Fc effector functions were attenuated more in BA.2 compared to BA.1.
Article
Nanoscience & Nanotechnology
Weiqiang Wang, Marcos Gil-Garcia, Salvador Ventura
Summary: Grafting biomolecules onto nanostructures' surfaces is a common strategy for targeting pathogenic cells with diagnostic and therapeutic applications. Multivalent nanomaterials for dual- or multitargeting are attracting significant interest due to the limitations of using monofunctionalized nanomaterials. This study presents a modular methodology for obtaining multivalent antibody-functionalized nanomaterials with multitargeting properties, showcasing potential for immunotherapy.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Immunology
Helen Byakwaga, Arturo Barbachano-Guerrero, Dongliang Wang, Shane McAllister, Kamal Naphri, Miriam Laker-Oketta, Conrad Muzoora, Peter W. Hunt, Jeffrey Martin, Christine A. King
Summary: In a study of untreated HIV-infected adults in Uganda, it was found that patients with KS had higher plasma IgE levels, which were associated with the presence and severity of KS. Higher eosinophil counts and plasma interleukin 33 concentrations were also linked to IgE levels, indicating a potential role of IgE-driven atopic inflammation in the pathogenesis of KS. Targeting IgE-mediated mast cell activation could be a novel approach for the treatment or prevention of KS.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Biophysics
Stefi V. Benjamin, Paul I. Creeke, Alistair J. Henry, James M. McDonnell
BIOMOLECULAR NMR ASSIGNMENTS
(2020)
Article
Biochemical Research Methods
Alkistis N. Mitropoulou, Tom Ceska, James T. Heads, Andrew J. Beavil, Alistair J. Henry, James M. McDonnell, Brian J. Sutton, Anna M. Davies
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
(2020)
Review
Allergy
D. Ferastraoaru, H. J. Bax, C. Bergmann, M. Capron, M. Castells, D. Dombrowicz, E. Fiebiger, H. J. Gould, K. Hartmann, U. Jappe, G. Jordakieva, D. H. Josephs, F. Levi-Schaffer, V Mahler, A. Poli, D. Rosenstreich, F. Roth-Walter, M. Shamji, E. H. Steveling-Klein, M. C. Turner, E. Untersmayr, S. N. Karagiannis, E. Jensen-Jarolim
CLINICAL AND TRANSLATIONAL ALLERGY
(2020)
Article
Biophysics
Yurema Teijeiro-Gonzalez, Alessandro Crnjar, Andrew J. Beavil, Rebecca L. Beavil, Jakub Nedbal, Alix Le Marois, Carla Molteni, Klaus Suhling
Summary: The study successfully investigated and interpreted homo-FRET between fluorescent proteins using a combination of time- and polarization-resolved fluorescence spectroscopy with molecular dynamics simulations, providing a powerful tool and new method for research in this field.
BIOPHYSICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Susan K. Vester, Rebecca L. Beavil, Steven Lynham, Andrew J. Beavil, Deborah S. Cunninghame Graham, James M. McDonnell, Timothy J. Vyse
Summary: The study identified nucleolin as a cell surface receptor of C1QTNF4, with interaction mediated by the second C1q-like domain of C1QTNF4 and the C terminus of nucleolin. C1QTNF4 primarily targets monocytes and B cells, actively internalizing upon cell binding. The findings contribute to a better understanding of C1QTNF4's role in the healthy immune system and its association with the development of systemic lupus erythematosus.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Veronica F. Ilkow, Anna M. Davies, Balvinder Dhaliwal, Andrew J. Beavil, Brian J. Sutton, James M. McDonnell
Summary: This study provides insights into the evolutionary differences between murine and human CD23, as well as highlighting some of the functional variances between CD23 in different species.
Article
Biochemical Research Methods
Caroline Pollard, Mark Hudson, James M. McDonnell, Paul G. Royall, Kim Wolff
Summary: A specific POCT for detecting MDMA in latent fingerprints has not been explored. The study aimed to design a sensitive POCT using SPR and LFA technology. By identifying a high-affinity antibody binding pair and testing titrations of fluorescently labelled antibody and antigen concentrations to allow clear distinction between negative and positive outcomes, a sensitive LFA screening tool was successfully designed. The tool showed better performance compared to a lower threshold of 40 pg/10 mu l.
DRUG TESTING AND ANALYSIS
(2022)
Article
Multidisciplinary Sciences
Minkoo Ahn, Tomasz Wlodarski, Alkistis Mitropoulou, Sammy H. S. Chan, Haneesh Sidhu, Elena Plessa, Thomas A. Becker, Nediljko Budisa, Christopher A. Waudby, Roland Beckmann, Anais M. E. Cassaignau, Lisa D. Cabrita, John Christodoulou
Summary: The structure and surface of the ribosome exit tunnel modulate the free energy of cotranslational protein folding through variations in protein loops. The shape of the tunnel, dynamic interactions between the ribosome surface and the unfolded nascent chain, and altered exit pathway influence the folding outcome.
NATURE COMMUNICATIONS
(2022)
Review
Immunology
J. M. McDonnell, B. Dhaliwal, B. J. Sutton, H. J. Gould
Summary: The evolution of IgE in mammals provided an additional layer of immune protection at body surfaces for rapid and local response against environmental antigens. The IgE immune response includes expulsive and inflammatory forces against local antigen stimulation, but it may also cause tissue damage and allergic disease. Two well-known IgE receptors, FceRI and CD23, mediate IgE activities. Unlike other antibody receptors, CD23 also regulates IgE expression to maintain IgE homeostasis. Recent research has revealed previously unknown mechanisms for regulation of IgE and IgE complexes, such as the dynamic structure of IgE and its allosteric modulation capacity.
ANNUAL REVIEW OF IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Anna M. Davies, Rebecca L. Beavil, Momchil Barbolov, Balraj S. Sandhar, Hannah J. Gould, Andrew J. Beavil, Brian J. Sutton, James M. McDonnell
Summary: This study reveals the three-dimensional structures of the Fab region of IgD, providing insights into the least well characterized mammalian immunoglobulin isotype. It identifies conformational diversity within the C81 domain and structural similarities between IgD and IgG, as well as differences with IgA and IgM, supporting predicted evolutionary relationships among mammalian antibody isotypes. The unique conformation of the upper hinge region in IgD Fab may contribute to the overall disposition of the long linker sequence between the Fab and Fc regions found in human IgD.
MOLECULAR IMMUNOLOGY
(2023)