Review
Acoustics
Qian Chen, Wei Qin, Weizhi Qi, Lei Xi
Summary: Photoacoustic imaging (PAI) is a rapidly growing biomedical imaging technology with handheld and semi-handheld devices for potential clinical applications. These devices include photoacoustic computed tomography (PACT), optoacoustic mesoscopy (OAMes), and photoacoustic microscopy (PAM), each with different laser delivery, scanning protocol, and acoustic detection methods. In addition to technical developments, associated clinical studies have been reviewed, and the advantages and disadvantages of these new techniques have been evaluated. Challenges and perspectives for miniaturized PAI in the future are also proposed.
Article
Physics, Applied
Daohuai Jiang, Hongbo Chen, Rui Zheng, Fei Gao
Summary: This paper presents a handheld free-scan 3D photoacoustic tomography (PAT) system based on a linear-array ultrasound probe. The system allows real-time 2D imaging and large field-of-view 3D volumetric imaging, reconstructed from multiple 2D images with coordinate information acquired using a global positioning system (GPS). The feasibility of the system is validated through simulation and experimental studies, and in vivo 3D imaging of human wrist vessels demonstrates its clinical potential.
JOURNAL OF APPLIED PHYSICS
(2022)
Article
Engineering, Electrical & Electronic
Eric Zou, Cheng Fang, Dezhen Song
Summary: This article presents the use of photoacoustics to detect watermelon ripeness. A new handheld PA probe is designed to measure rind thickness and inner flesh redness, two visual hallmarks of watermelon ripeness. Data acquisition and machine learning techniques are employed to estimate the ripeness of watermelons. The results show that the PA probe is a promising solution for rapid and non-destructive ripeness detection.
IEEE SENSORS JOURNAL
(2023)
Article
Optics
Daewoon Seong, Sangyeob Han, Jaeyul Lee, Euimin Lee, Yoonseok Kim, Junsoo Lee, Mansik Jeon, Jeehyun Kim
Summary: Photoacoustic imaging is a non-invasive technique merging high optical contrast and high acoustic resolution in deep tissues. The development of a waterproof, handheld PAM system with extended field of view has improved its versatility for wide-range imaging applications. Studies confirm the feasibility and applicability of the system for various biomedical imaging purposes.
Article
Chemistry, Analytical
Chiho Yoon, Changyeop Lee, Keecheol Shin, Chulhong Kim
Summary: This paper presents a new motion compensation method for removing motion artifacts in photoacoustic imaging, and its effectiveness is verified through experiments. This method has significant implications for clinical applications of photoacoustic imaging.
Article
Chemistry, Analytical
Xiaoxia Chen, Xingxing Ren, Yuhan Zhu, Ziyan Fan, Lele Zhang, Zhengjie Liu, Ling Dong, Zijuan Hai
Summary: The study focused on the development of two CTB-activated FL/PA probes, demonstrating high sensitivity and spatial resolution in monitoring CTB activity in vivo. HCy-Cit-Val showed superior performance and is envisioned to be suitable for clinical early diagnosis of CTB-related cancer in the near future.
ANALYTICAL CHEMISTRY
(2021)
Article
Acoustics
Changyeop Lee, Seonghee Cho, Donghyun Lee, Jonghun Lee, Jong-Il Park, Hong-Ju Kim, Sae Hyun Park, Wonseok Choi, Ung Kim, Chulhong Kim
Summary: This study proposes a panoramic volumetric clinical photoacoustic and ultrasound imaging system for imaging vascular networks and measuring hemoglobin oxygen saturation levels. The system achieves large field-of-view imaging through multiple photoacoustic and ultrasound scans, which are mosaic-stitched after correction. The findings of this study have important implications for various clinical fields such as cardiovascular imaging, dermatology, vascular surgery, internal medicine, and oncology.
Article
Optics
Jiaying Xiao, Jinsheng Jiang, Jiaxi Zhang, Yongjun Wang, Bo Wang
Summary: The study of molecular photoacoustic endoscopic imaging in deep tissues is limited due to technical difficulties. In this work, a multimodal acoustic-resolution-based PAE system was set up to image the rabbit rectum and explore the potential of molecular PAE for deep-seated targets. The results show that the system has achieved high resolution and signal-to-noise ratio at an imaging depth of 1.4 cm, and can distinguish the rectum wall and indocyanine green.
Article
Chemistry, Analytical
Yi Qi, Ruochong Zhang, Poongkulali Rajarahm, Shuyan Zhang, Amalina Binte Ebrahim Attia, Renzhe Bi, Malini Olivo
Summary: In this study, a portable confocal Raman spectroscopy system with a simultaneous dual-wavelength source and a miniaturized handheld probe was developed. The system can acquire spectra in both fingerprint and high wavenumber regions simultaneously. An innovative design and a unique Raman spectra separation algorithm were implemented to improve the efficiency and accuracy of spectra acquisition.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yi Qi, Ruochong Zhang, Poongkulali Rajarahm, Shuyan Zhang, Amalina Binte Ebrahim Attia, Renzhe Bi, Malini Olivo
Summary: In this study, a portable confocal Raman spectroscopy (CRS) system with a simultaneous dual-wavelength source and a miniaturized handheld probe was developed. A novel algorithm was also developed for accurate spectral separation. The results demonstrated that the proposed CRS system and algorithm greatly improve the integration of CRS in the clinical workflow.
ANALYTICAL CHEMISTRY
(2023)
Article
Optics
Paul R. Torke, Robert Nuster, Guenther Paltauf
Summary: This study demonstrates an alternative approach for photoacoustic imaging using illumination patterns obtained from a Hadamard matrix. The generated ultrasound waves are measured with a single detector and reconstructed using computational ghost imaging. Compared to raster scan, this method provides better contrast-to-noise ratio by illuminating multiple pixels simultaneously and allows for depth-resolved imaging by exploiting temporal information. It is particularly valuable in situations where sample radiant exposure is limited due to safety precautions or properties of the light source.
Article
Optics
Zhan Pang, Yike Wang, Wei Qin, Weizhi Qi, Lei Xi
Summary: This study developed a handheld volumetric photoacoustic/ultrasound (PA/US) imaging probe with a special light-ultrasound coupling design and an internal scanning mechanism. The probe achieved optimized signal-to-noise ratio (SNR) and a large field of view (FOV) for deep tissue imaging through a coaxial design and rotational acoustic reflectors. In vivo human trials demonstrated the high imaging quality and stability of the system for potential clinical translations.
Article
Multidisciplinary Sciences
Yizhi Liang, Wubing Fu, Qiang Li, Xiaolong Chen, Huojiao Sun, Lidai Wang, Long Jin, Wei Huang, Bai-Ou Guan
Summary: Photoacoustic endoscopy with a miniaturized, optical-resolution sensor shows promise in the detection of gastrointestinal cancer and inflammation. The sensor utilizes acoustic-induced optical phase change to enhance the acoustic response and offers resistance to thermal drift and vibrational perturbations through heterodyne phase detection using dual-frequency laser beams. In vivo imaging of a rat rectum with the endoscope allows visualization of oxygen saturation changes during acute inflammation, which is difficult to observe with other imaging modalities.
NATURE COMMUNICATIONS
(2022)
Article
Optics
Shui-Jing Tang, Mingjie Zhang, Jialve Sun, Jia-Wei Meng, Xiao Xiong, Qihuang Gong, Dayong Jin, Qi-Fan Yang, Yun-Feng Xiao
Summary: Researchers demonstrate real-time measurement of natural vibrations of single mesoscopic particles using photoacoustic excitation and acoustic coupling to an optical microresonator for readout. This technology extends the reach of vibrational spectroscopy and is applied for biomechanical fingerprinting of microorganisms at the single-cell level.
Article
Optics
Rui Cao, Jingjing Zhao, Lei Li, Lin Du, Yide Zhang, Yilin Luo, Laiming Jiang, Samuel Davis, Qifa Zhou, Adam de la Zerda, Lihong Wang
Summary: Optical-resolution photoacoustic microscopy can visualize wavelength-dependent optical absorption at the cellular level. However, it has a limited depth of field. To overcome this limitation, needle-shaped beam photoacoustic microscopy is proposed. This approach extends the depth of field and provides new perspectives for slide-free intraoperative pathological imaging and in vivo organ-level imaging.
Review
Medicine, Research & Experimental
Eun-Yeong Park, Haeni Lee, Seongyi Han, Chulhong Kim, Jeesu Kim
Summary: Photoacoustic imaging combines the advantages of optical and acoustic modalities, providing strong optical contrast and high ultrasound resolution in deep tissue. It can visualize complementary information to ultrasound imaging, making it a valuable tool for clinical applications.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2022)
Review
Chemistry, Multidisciplinary
Wonseok Choi, Byullee Park, Seongwook Choi, Donghyeon Oh, Jongbeom Kim, Chulhong Kim
Summary: Photoacoustic imaging (PAI) has been investigated for decades as a niche biomedical imaging modality. Despite its desirable contrast and resolution, PAI faces limitations such as low signal-to-noise ratio, diminished image contrast, and spatial resolution constraints in deep tissue. Practical limitations, including insufficient targeting and difficulties in developing clinically translatable agents, have also been identified. This review discusses recent research efforts in addressing these challenges and proposes future directions for contrast-enhanced PAI.
Article
Optics
Wangyu Kim, Wonseok Choi, Joongho Ahn, Changyeop Lee, Chulhong Kim
Summary: In this study, a dual-modal PA and US imaging system using a 2D matrix transducer array and a motorized 2D scanning system was demonstrated. The system enlarged the field-of-view of volumetric images by merging multiple PA volumes to form a wide-field image. In vivo imaging of rat sentinel lymph nodes and bladders stained with methylene blue was achieved. The technique has the potential for real-time monitoring and wide-field imaging in various preclinical and clinical studies.
Article
Biochemical Research Methods
Jinhee Yoo, Donghyeon Oh, Chulhong Kim, Hyung Ham Kim, Ji-Yong Um
Summary: Photoacoustic (PA) imaging is a high-fidelity biomedical imaging technique that utilizes molecular-specific optical absorption in biological tissue. By introducing a switchable preamplifier, the signal-to-noise ratio (SNR) of PA imaging can be selectively enhanced, allowing for better visualization of deep tissues. This study demonstrates the application of the preamplifier in in vivo whole-body PA/US imaging, showing improved SNR and revealing deeply located organs and vascular networks. The switchable amplifier strengthens the mutual complement between PA/US imaging and can potentially address the imbalance in image depth between the two modalities.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Chemistry, Multidisciplinary
Seongwook Choi, Jinge Yang, Soo Young Lee, Jiwoong Kim, Jihye Lee, Won Jong Kim, Seungchul Lee, Chulhong Kim
Summary: This study demonstrates a deep learning-based approach to reduce limited-view artifacts and improve image quality and temporal resolution in photoacoustic computed tomography (PACT). The approach, called DL-PACT, enables high-quality imaging in a fast and cost-effective manner, which is significant for various clinical applications such as neurology, cardiology, pharmacology, endocrinology, and oncology.
Article
Engineering, Biomedical
Taejeong Kim, Hyun Jin Kim, Wonseok Choi, Yeong Mi Lee, Jung Hyun Pyo, Junseok Lee, Jeesu Kim, Jihoon Kim, Joung-Hun Kim, Chulhong Kim, Won Jong Kim
Summary: Deep brain stimulation through implanted electrodes is effective for neuronal disorders, but limited by side effects. This study demonstrates that systemically administered piezoelectric nanoparticles, activated by high-intensity focused ultrasound, can stimulate deep brain tissue without direct brain electrode insertion. The nanoparticles temporarily disrupt the blood-brain barrier and stimulate dopamine release, effectively alleviating symptoms in a Parkinson's disease mouse model. This strategy has potential for developing minimally invasive therapies for neurodegenerative diseases.
NATURE BIOMEDICAL ENGINEERING
(2023)
Review
Chemistry, Multidisciplinary
Seongyi Han, Tsedendamba Ninjbadgar, Mijeong Kang, Chulhong Kim, Jeesu Kim
Summary: Photoacoustic agents have wide applications in theranostic field. By evaluating the biodistribution obtained from photoacoustic images, the efficiency and response of theranostic agents can be analyzed. This study summarizes the recent advances in photoacoustic-guided phototherapy, providing guidance for future development of theranostic agents. With the recent applications of photoacoustic imaging in clinical trials, theranostic agents with photoacoustic monitoring have potential for clinical translation.
Article
Acoustics
Joongho Ahn, Jin Woo Baik, Donggyu Kim, Karam Choi, Seunghyun Lee, Sung-Min Park, Jin Young Kim, Sung Hyun Nam, Chulhong Kim
Summary: Postprandial hyperglycemia induces endothelial dysfunction, leading to increased cardiovascular risks. This study demonstrates the use of photoacoustic microscopy to noninvasively monitor morphological changes in blood vessels during acute hyperglycemia. The results show that acute hyperglycemia causes transient vasoconstriction in arterioles, with an opposite trend of change in blood glucose. These findings provide insights into vascular glucose homeostasis and the relationship between diabetes and cardiovascular diseases.
Article
Acoustics
Changyeop Lee, Seonghee Cho, Donghyun Lee, Jonghun Lee, Jong-Il Park, Hong-Ju Kim, Sae Hyun Park, Wonseok Choi, Ung Kim, Chulhong Kim
Summary: This study proposes a panoramic volumetric clinical photoacoustic and ultrasound imaging system for imaging vascular networks and measuring hemoglobin oxygen saturation levels. The system achieves large field-of-view imaging through multiple photoacoustic and ultrasound scans, which are mosaic-stitched after correction. The findings of this study have important implications for various clinical fields such as cardiovascular imaging, dermatology, vascular surgery, internal medicine, and oncology.
Review
Nanoscience & Nanotechnology
Byullee Park, Donghyeon Oh, Jeesu Kim, Chulhong Kim
Summary: Functional photoacoustic imaging is a promising biological imaging technique that provides scalable resolution, imaging depth, and functional information. It can capture super-resolution images at the nanoscale and measure various physiological parameters at the microscopic and macroscopic scales. This comprehensive review discusses recent advances in technology and applications, and explores the future prospects of functional photoacoustic imaging in the biomedical field.
Review
Medicine, Research & Experimental
Haeni Lee, Wonseok Choi, Chulhong Kim, Byullee Park, Jeesu Kim
Summary: Photoacoustic imaging has been developed as a new biomedical molecular imaging modality and has been combined with ultrasound imaging to visualize physiological and morphological information in biological systems. Dual-modal photoacoustic and ultrasound imaging showed synergistic advances in photoacoustic imaging with the guidance of ultrasound images. This review presents the recent progresses in dual-modal photoacoustic and ultrasound imaging systems at various study scales and summarizes different strategies for combining ultrasound structural information with photoacoustic molecular information.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2023)
Article
Computer Science, Artificial Intelligence
Chiho Yoon, Sampa Misra, Kwang-Ju Kim, Chulhong Kim, Bum Joon Kim
Summary: In this study, a deep learning method was used to classify patients requiring EVT within 6 hours. A DL-based segmentation model was developed to predict the ROI from multi-modal MR images, and radiomic and deep features were extracted. The results showed that combining radiomic and deep features improved the classification performance, with an F0.5 score of 80.6% achieved in classifying SOT within 6 hours. The DL model using multi-modal MR images has the potential to become a practical decision-support tool for stroke treatments.
EXPERT SYSTEMS WITH APPLICATIONS
(2023)
Review
Chemistry, Analytical
Changyeop Lee, Chulhong Kim, Byullee Park
Summary: Photoacoustic imaging is a non-invasive biomedical imaging technique that combines optics and acoustics. This review focuses on the emergence of three-dimensional handheld PA imaging systems and their potential applications in the biomedical field. The review provides an overview of different imaging techniques and discusses potential solutions for system limitations.
Article
Chemistry, Multidisciplinary
Hyori Lee, Byullee Park, Jihye Lee, Yeoul Kang, Moongyu Han, Junseok Lee, Chulhong Kim, Won Jong Kim
Summary: This study designs a charge-convertible nanomedicine that facilitates deep penetration into solid tumors via transcytosis, providing a versatile theragnosis platform for noninvasive photoacoustic imaging and high therapeutic efficiency. The nanomedicine is an albumin-based calcium phosphate nanomedicine loaded with IR820 for high-resolution photoacoustic imaging and enhanced photothermal therapy. The pH-triggered transcytosis of the nanomedicine enabled by caveolae-mediated endocytosis and calcium ion-induced exocytosis is demonstrated in cellular, spheroid, and in vivo tumor models.
Article
Chemistry, Multidisciplinary
Donghyeon Oh, Donghyun Lee, Jinseok Heo, Jooyoung Kweon, Uijung Yong, Jinah Jang, Yong Joo Ahn, Chulhong Kim
Summary: A new non-invasive imaging system has been developed to assess renal microcirculation. The system allows researchers to observe the entire 3D vascular network in a living rat's kidney at a high resolution without using any contrast agent. By monitoring the kidneys with acute kidney injury and diabetic kidney disease, hemodynamic and morphological changes can be quantitatively measured. This study provides valuable insights for future research in diabetes and kidney transplantation.
