Article
Multidisciplinary Sciences
Bastien Bissaro, Bennett Streit, Ingvild Isaksen, Vincent G. H. Eijsink, Gregg T. Beckham, Jennifer L. DuBois, Asmund K. Rohr
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Multidisciplinary Sciences
Bastien Bissaro, Eirik Kommedal, Asmund K. Rohr, Vincent G. H. Eijsink
NATURE COMMUNICATIONS
(2020)
Article
Multidisciplinary Sciences
Leszek Michalak, Sabina Leanti La Rosa, Shaun Leivers, Lars Jordhoy Lindstad, Asmund Kjendseth Rohr, Finn Lillelund Aachmann, Bjorge Westereng
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Biotechnology & Applied Microbiology
Anton A. Stepnov, Zarah Forsberg, Morten Sorlie, Giang-Son Nguyen, Alexander Wentzel, Asmund K. Rohr, Vincent G. H. Eijsink
Summary: The study describes a novel two-domain cellulose-active family AA10 LPMO from a marine actinomycete, and investigates the effects of the reductant and copper ions on the LPMO reaction. Results show that ascorbate-driven LPMO reactions are extremely sensitive to very low amounts of free copper, whereas reactions driven by gallic acid are almost unaffected by the presence of free copper ions.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Multidisciplinary Sciences
Fatemeh Askarian, Satoshi Uchiyama, Helen Masson, Henrik Vinther Sorensen, Ole Golten, Anne Cathrine Bunaes, Sophanit Mekasha, Asmund Kjendseth Rohr, Eirik Kommedal, Judith Anita Ludviksen, Magnus O. Arntzen, Benjamin Schmidt, Raymond H. Zurich, Nina M. van Sorge, Vincent G. H. Eijsink, Ute Krengel, Tom Eirik Mollnes, Nathan E. Lewis, Victor Nizet, Gustav Vaaje-Kolstad
Summary: The Pseudomonas aeruginosa lytic polysaccharide monooxygenase CbpD is a chitin-oxidizing virulence factor that promotes bacterial survival in human blood, with its catalytic activity promoted by azurin and pyocyanin. Deletion of CbpD hinders the bacterium's ability to establish lethal systemic infection and leads to increased bacterial clearance. CbpD's key role in virulence is attributed to its ability to attenuate the terminal complement cascade in human serum.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Marianne Stabell, Thomas Saether, Asmund K. Rohr, Odd S. Gabrielsen, Ola Myklebost
Summary: Through methylation of a lysine residue in the KAE SUMOylation motif of HMGA2, the sequence is transformed into a consensus SUMO motif, leading to increased SUMOylation at that site. Similar methylation-dependent SUMO motifs are found in other chromatin factors, suggesting that crosstalk between methylation and SUMOylation is a general mode for regulating chromatin function.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Cardiac & Cardiovascular Systems
Mohammed Shah, Zhenhe He, Ali Rauf, Siavash B. Kalkhoran, Christina Mathisen Heiestad, Kare-Olav Stenslokken, Christopher R. Parish, Oliver Soehnlein, Sapna Arjun, Sean M. Davidson, Derek Yellon
Summary: This study found that histones released during myocardial infarction are toxic to cardiomyocytes, and this toxicity is independent of TLR4. Inhibiting the release of histones can reduce cardiomyocyte death, providing a new therapeutic opportunity for ischemia-reperfusion injury.
CARDIOVASCULAR RESEARCH
(2022)
Article
Microbiology
Lars J. Lindstad, Galiana Lo, Shaun Leivers, Zijia Lu, Leszek Michalak, Gabriel V. Pereira, Asmund K. Rohr, Eric C. Martens, Lauren S. McKee, Petra Louis, Sylvia H. Duncan, Bjorge Westereng, Phillip B. Pope, Sabina Leanti La Rosa
Summary: The study demonstrates that the dominant butyrate producer Faecalibacterium prausnitzii in the human gut is able to acquire and degrade various beta-mannooligosaccharides, contributing to a more comprehensive understanding of beta-mannan metabolism and the cross-feeding interactions between beneficial microbes in the human gut. The findings highlight the importance of microbial metabolism of beta-mannans/beta-MOS as a common dietary component, offering potential insights for developing dietary formulations that may boost this beneficial symbiont and butyrate production in the gut.
Article
Biochemistry & Molecular Biology
Fredrik Gjerstad Stopamo, Asmund Kjendseth Rohr, Sophanit Mekasha, Dejan M. Petrovic, Aniko Varnai, Vincent G. H. Eijsink
Summary: The study reports the structural and functional characteristics of a single-domain AA11 LPMO from Aspergillus fumigatus. The LPMO, AfAA11A, shows substrate-binding surface features similar to those of known chitin-active LPMOs and exhibits considerable affinity for both alpha-chitin and beta-chitin, enhancing chitin degradation.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Sofie S. Kristensen, Thomas F. Oftedal, Asmund K. Rohr, Vincent G. H. Eijsink, Geir Mathiesen, Dzung B. Diep
Summary: This study investigates the molecular mechanisms underlying the interaction between RseP and Enterocin K1 (EntK1) and the bacteriocin sensitivity. Through mutational analysis, the researchers identified key regions and motifs of RseP involved in bacteriocin binding and activity. They found that the extracellular PDZ domain is the main determinant of EntK1 sensitivity. These findings may contribute to the development of novel bacteriocins and the identification of RseP as a potential antibacterial target.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Ole Golten, Ivan Ayuso-Fernandez, Kelsi R. Hall, Anton A. Stepnov, Morten Sorlie, asmund Kjendseth Rohr, Vincent G. H. Eijsink
Summary: The catalytic activity of polysaccharide-degrading mono-copper lytic polysaccharide monooxygenases (LPMOs) is affected by the choice of reductant and pH. The reaction rate with ascorbic acid is not pH-dependent between pH 6.0 and 8.0, while the reaction rate with gallic acid increases at higher pH. This correlation is attributed to the ionization state of the reductant, which affects its ability to generate H2O2 from molecular oxygen. However, l-cysteine shows a different behavior due to increased binding to the copper active site at higher pH, counteracting the increase in H2O2 production.
Article
Chemistry, Multidisciplinary
Kelsi R. Hall, Chris Joseph, Ivan Ayuso-Fernandez, Ashish Tamhankar, Lukas Rieder, Rannei Skaali, Ole Golten, Frank Neese, Asmund K. Rohr, Sergio A. V. Jannuzzi, Serena DeBeer, Vincent G. H. Eijsink, Morten Sorlie
Summary: By mutating a conserved glutamine/glutamate in Lytic polysaccharide monooxygenases (LPMOs), it was found that the nature and distance of the headgroup to the copper can fine-tune the functionality and reactivity of LPMOs. The presence of glutamate near the copper decreased the reduction potential and the ratio between reduction and reoxidation rates. These findings provide important insights for understanding LPMOs and LPMO-inspired synthetic catalysts.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Meeting Abstract
Biochemistry & Molecular Biology
G. Valerio, A. K. Rohr, J. J. G. Moura, C. Cordas
Meeting Abstract
Biochemistry & Molecular Biology
C. M. Cordas, G. N. Valerio, A. K. Rohr, V. G. H. Eijsink, J. J. G. Moura
Meeting Abstract
Chemistry, Multidisciplinary
Marta Hammerstad, Ingvild Gudim, Marie Lofstad, Kjendseth Asmund Rohr, Hans-Petter Hersleth
ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES
(2019)
