Article
Biochemistry & Molecular Biology
Yonghua Jiang, Yingwei Qi, Xilong Chen, Qian Yan, Jiezhen Chen, Hailun Liu, Fachao Shi, Yingjie Wen, Changhe Cai, Liangxi Ou
Summary: The acidity of fruits is determined by their organoleptic quality and nutritive value. In litchi fruits, the variation in acidity is mainly associated with malate and citrate metabolisms, and genes such as LITCHI026501.m2, LITCHI020148.m5, and LITCHI003343.m3 play important roles. These findings have significant implications for improving the quality of litchi fruits.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Li Yang, Chuanji Zhao, Zetao Bai, Lingli Yang, M. Eric Schranz, Shengyi Liu, Klaas Bouwmeester
Summary: This study compared two Brassica napus lines with different resistance levels to black rot disease and evaluated the differential gene expression during infection. The resistant line showed a gradual increase in differentially expressed genes (DEGs), while the susceptible line displayed a steady amount of DEGs. Analysis of gene co-expression networks identified multiple defense-related genes and enriched pathways associated with defense.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Sihao Hou, Tiantian Zhao, Zhen Yang, Lisong Liang, Wenxu Ma, Guixi Wang, Qinghua Ma
Summary: This study elucidated the mechanism of self-incompatibility and pollen-stigma interactions in Corylus plants through transcriptomic analysis, and identified key genes potentially involved in self-incompatibility. Additionally, the S-locus region was identified, laying the foundation for further analyses.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Dan Wang, Jietang Zhao, Yaqi Qin, Yonghua Qin, Guibing Hu
Summary: Sucrose synthase (SUS) is a key enzyme in plant sucrose metabolism. In this study, five SUS genes were identified in litchi, sharing high similarity in sequences. These genes were classified into three groups, showing evolutionary conservation. Real-time PCR analysis revealed different expression patterns of these genes in various tissues and developmental stages, indicating their roles in regulating sugar accumulation in litchi aril.
HORTICULTURAL PLANT JOURNAL
(2021)
Article
Genetics & Heredity
Yanjie Hou, Xianquan Qin, Hongye Qiu, Dongbo Li, Ning Xu, Shuwei Zhang, Chen Fang, Hongli Li
Summary: Litchi is an important commercial fruit crop, but graft incompatibility is a major obstacle for its large-scale cultivation. This study analyzed the metabolic and transcriptomic differences between graft compatible and incompatible rootstock-scion combinations of litchi. The results provide insights into the molecular mechanism of graft incompatibility in litchi.
FRONTIERS IN GENETICS
(2023)
Article
Multidisciplinary Sciences
Balazs Kalapos, Csilla Juhasz, Eszter Balogh, Gabor Kocsy, Istvan Tobias, Gabor Gullner
Summary: The study found significant differences in the impact of two tobamoviruses on the transcriptome of pepper, with Obuda pepper virus markedly suppressing genes related to photosynthesis while PMMoV had minimal effect on disease signaling and defense pathways. This provides a deeper insight into the defense mechanisms underlying tobamovirus resistance in pepper.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Sheetal M. Bhosle, Ragiba Makandar
Summary: The study conducted comparative transcriptome analysis of pea genotypes infected with Erysiphe pisi to detect molecular components involved in compatible and incompatible interactions. Significant increase in gene expression related to defense and hormonal signaling was observed in the resistant genotype.
FEMS MICROBIOLOGY ECOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Junren Wen, Yong Sui, Shuyi Li, Jianbin Shi, Sha Cai, Tian Xiong, Fang Cai, Lei Zhou, Shengnan Zhao, Xin Mei
Summary: In this study, the biotransformation of lotus seedpod and litchi pericarp procyanidins by LABs was analyzed. The results showed that LABs could degrade procyanidins into small molecular compounds and enhance the antioxidant capacity of certain flavonoids.
Article
Immunology
Julia Cecilia Madela-Moenchinger, Silver Anthony Wolf, Emanuel Wyler, Agnieszka Bauer, Marius Mischke, Lars Moeller, Vanda Juranic Lisnic, Markus Landthaler, Anna Malyshkina, Sebastian Voigt
Summary: Dendritic cells (DC) play a crucial role in antiviral immunity, but can also be infected by Cytomegalovirus (CMV). This study investigates the infection of rat CMV on XCR1+ DC and its effects on cell surface markers and migration behavior. The results show that RCMV infection reduces XCR1 and MHCII surface expression, reverts DC activation, and impairs the chemotactic activity of infected DC.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Priyadarshini Rout, Nihal Ravindranath, Dinkar Gaikwad, Satyabrata Nanda
Summary: This study analyzed the transcriptomic, physiological, and gene-silencing responses of the BPH biotype 4 during compatible and incompatible rice-BPH interactions. It was found that in the incompatible interaction, there was a significant reduction in honeydew production and negative weight gain in BPH. Additionally, the trehalose and glucose contents were significantly high and low, respectively, during the incompatible interaction. The study also identified differentially expressive genes involved in vital BPH physiological processes.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Horticulture
Le Van Trong, Nguyen Nhu Khanh, Le Thi Huyen, Ha Thi Phuong, Vu Thi Thu Hien
Summary: The study examined the changes in physiological indicators of litchi fruit grown in Vietnam during growth and development. It was found that harvesting litchi fruit at 10 weeks after anthesis is crucial for preserving its nutritional value during storage.
REVISTA BRASILEIRA DE FRUTICULTURA
(2021)
Article
Biotechnology & Applied Microbiology
Xufang Shen, Hongwei Yan, Jieming Jiang, Weiyuan Li, Yuyu Xiong, Qi Liu, Ying Liu
Summary: The study compared the differential transcriptomic responses of the Takifugu rubripes larvae brain after E-2 treatment, revealing numerous differentially expressed genes and enriched metabolic pathways and signaling pathways. This provides important clues for further research on the possible involvement of the brain-pituitary-gonadal axis network in sex differentiation in T. rubripes.
Article
Microbiology
Ling Sun, Li-Ping Liu, Ya-Zhen Wang, Lei Yang, Cunsheng Zhang, Mei-Xiang Yue, Mokhtar Dabbour, Benjamin Kumah Mintah, Liang Wang
Summary: This study investigated the effect of ultrasonication on the liquid fermentation of Ganoderma lucidum, showing significant changes in metabolic and transcriptional levels. Ultrasonication induced more metabolites biosynthesis and lasting influence on gene expression in mycelia. The enhanced triterpenoids production of G. lucidum by up-regulating terpenoids synthase genes suggests ultrasound as an efficient approach for producing more metabolites.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Fisheries
Xuemei Tang, Shulun Jiang, Henglin Wang, Yanfeng Zhou, Fei Peng, Xizhao Zhang, Yifan Zhou, Shiyue Guo, Yang You
Summary: This study investigated the dynamic changes in biological functions during the early development of clearhead icefish through transcriptome sequencing and different analysis strategies. It found that genes related to digestion and feeding regulation gradually increased during early development, and identified enriched pathways associated with eye, bone, heart development, and metabolism at different stages.
Article
Biochemical Research Methods
Zhe-Xin Li, Wen-Lin Zhang, Deng-Wei Jue, Xia Liu, Yu-Song Jiang, Jian-Min Tang
Summary: Kiwifruit is rich in dietary fiber and vitamin C, but it is susceptible to Botrytis cinerea pathogenesis. This study found that the fungus caused changes in enzyme activity and identified thousands of differentially expressed genes. Certain gene families and metabolic pathways were also found to be involved in the response to B. cinerea. The hub genes identified in this study could be potential targets for breeding kiwifruit with improved resistance to biotic stress.
PLANT MOLECULAR BIOLOGY REPORTER
(2022)
Article
Genetics & Heredity
Jietang Zhao, Linhuan Chen, Anna Ma, Dan Wang, Hanle Lu, Lei Chen, Huicong Wang, Yonghua Qin, Guibing Hu
Summary: The repressor of anthocyanin synthesis, LcMYBx, inhibits the accumulation of anthocyanin in plants by interacting with bHLH proteins, resulting in faded petals.
Article
Biochemistry & Molecular Biology
Xinglong Hu, Fangfang Xie, Wenwei Liang, Yinhao Liang, Zhike Zhang, Jietang Zhao, Guibing Hu, Yonghua Qin
Summary: NAC transcription factors are a large family of plant regulators that play important roles in plant growth and stress responses. This study identified 64 NAC genes in pitaya and analyzed their characteristics and functions. The study also identified candidate genes involved in cold stress tolerance and provided valuable information for breeding cold-tolerant pitaya varieties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Canbin Chen, Fangping Li, Fangfang Xie, Jiaxuan Chen, Qingzhu Hua, Jianye Chen, Zhijiang Wu, Zhike Zhang, Rong Zhang, Jietang Zhao, Guibing Hu, Yonghua Qin
Summary: Pitaya, a novel fruit with a delicious taste and superior horticulture ornamental value, has diverse uses in agricultural produce, processed foods, industrial and medicinal products. It also serves as an excellent plant material for biological research. A comprehensive database of pitaya has been constructed, providing genomic, gene expression, miRNA, metabolite, and proteomic data from different pitaya cultivars. The database also includes videos on flowering process and planting tutorials. These valuable data will significantly facilitate future studies on pitaya genetics, breeding, and function research.
Article
Plant Sciences
Fangfang Xie, Canbin Chen, Jiaxuan Chen, Yuanju Yuan, Qingzhu Hua, Zhike Zhang, Jietang Zhao, Guibing Hu, Jianye Chen, Yonghua Qin
Summary: This study examined the sugar and organic acid metabolism in pulp of yellow-peel pitayas and identified genes involved in these processes. The results highlighted the changes in sugar and organic acid contents during fruit development stages and revealed the key genes related to sugar and organic acid metabolism in pitayas. This study provides new insights for regulating the quality of pitayas.
Article
Biochemistry & Molecular Biology
Canbin Chen, Fangfang Xie, Kamran Shah, Qingzhu Hua, Jiayi Chen, Zhike Zhang, Jietang Zhao, Guibing Hu, Yonghua Qin
Summary: This study reports the characterization and function of 70 WRKY genes in pitaya. It is found that HmoWRKY42 acts as a transcriptional repressor and regulates pitaya betalain biosynthesis by inhibiting the transcription of HmocDOPA5GT1. These findings provide a foundation for future research on the regulation and functions of the HuWRKY gene family.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Fangfang Xie, Canbin Chen, Jiayi Chen, Jiaxuan Chen, Qingzhu Hua, Kamran Shah, Zhike Zhang, Jietang Zhao, Guibing Hu, Jianye Chen, Yonghua Qin
Summary: In this study, a 1R-MYB gene named HuMYB132 was identified in red-pulp pitaya. It was found to positively regulate the synthesis of betalain in pitaya by promoting the transcriptional activities of HuADH1, HuCYP76AD1-1, and HuDODA1. This research provides new insights into the regulation of betalain biosynthesis in pitaya.
Article
Plant Sciences
Dan Wang, Lei Chen, Yabing Yang, Farhat Abbas, Yaqi Qin, Hanle Lu, Biao Lai, Zichen Wu, Bing Hu, Yonghua Qin, Huicong Wang, Jietang Zhao, Guibing Hu
Summary: Anthocyanins, health-promoting compounds with strong antioxidant properties, accumulate in the pericarp but not in the aril of litchi fruit. Metabolome and transcriptome analyses revealed that a block in cyanidin glycosylation is responsible for the deficiency of anthocyanin biosynthesis in the aril. Additionally, 9 candidate genes, including LcMYB1, were identified to play a significant role in regulating anthocyanin biosynthesis. This study provides insights into the molecular mechanism of tissue-specific anthocyanin accumulation and will contribute to the development of new red-fleshed litchi germplasm.
PHYSIOLOGIA PLANTARUM
(2023)
Correction
Biochemical Research Methods
Yaqi Qin, Dan Wang, Jiaxin Fu, Zhike Zhang, Yonghua Qin, Guibing Hu, Jietang Zhao
Correction
Plant Sciences
Canbin Chen, Fangfang Xie, Qingzhu Hua, Noemi Tel-Zur, Lulu Zhang, Zhike Zhang, Rong Zhang, Jietang Zhao, Guibing Hu, Yonghua Qin
Article
Plant Sciences
Jianmei Zeng, Jiayi Chen, Kamran Shah, Fangfang Xie, Canbin Chen, Jiaxuan Chen, Jietang Zhao, Guibing Hu, Zhike Zhang, Yonghua Qin
Summary: In this study, 16 HuSPL genes were identified from the pitaya genome, which were distributed unevenly among nine chromosomes. It was found that these genes could be targeted by Hmo-miR156/157b and exhibited differential expression patterns during fruit maturation. HuSPL12 could inhibit the expression of HuWRKY40 by binding to its promoter and interact with HuMYB1, HuMYB132, or HuWRKY42, which are responsible for betalain biosynthesis.
PHYSIOLOGIA PLANTARUM
(2023)
Article
Biochemistry & Molecular Biology
Jiayi Chen, Fangfang Xie, Kamran Shah, Canbin Chen, Jianmei Zeng, Jiaxuan Chen, Zhike Zhang, Jietang Zhao, Guibing Hu, Yonghua Qin
Summary: In this study, 165 HubHLH genes were identified, and their functions related to betalain biosynthesis in pitaya were revealed. HubHLH159 gene showed high expression level in red-pulp pitaya, and promoted betalain biosynthesis by activating the expression of HuADH1, HuCYP76AD1-1, and HuDODA1.
Article
Plant Sciences
Sha Li, Yaqi Qin, Shiqi Jing, Dan Wang, Zhike Zhang, Yonghua Qin, Guibing Hu, Jietang Zhao
Summary: Agrobacterium rhizogenes-mediated hairy root culture is a useful method for gene function analysis and plant secondary metabolite production. In this study, red litchi hairy roots were successfully obtained by transforming with A. rhizogenes-mediated LcMYB1. Anthocyanin profiling and transcriptome analysis revealed significant changes in metabolites and gene expression associated with flavonoid biosynthesis. Key structural genes in anthocyanin biosynthesis were identified, while genes involved in lignin production were down-regulated, indicating a competition between flavonoids and lignin in the phenylpropanoid pathway. Several transcription factors were also found to play a role in regulating anthocyanin biosynthesis in litchi hairy roots. These findings provide insights into the molecular mechanisms of anthocyanin accumulation in litchi hairy roots regulated by LcMYB1.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Forestry
Jun-Wen Yi, Han-Tao Ge, Farhat Abbas, Jie-Tang Zhao, Xu-Ming Huang, Gui-Bing Hu, Hui-Cong Wang
Summary: The level of glucose is negatively correlated with fruit abscission in litchi. Furthermore, LcHXK1 is identified as a key regulator in fruit abscission through its function as a hexose sensor.
Article
Plant Sciences
Zhike Zhang, Yemiao Xing, Muthusamy Ramakrishnan, Canbin Chen, Fangfang Xie, Qingzhu Hua, Jianye Chen, Rong Zhang, Jietang Zhao, Guibing Hu, Yonghua Qin
Summary: This study investigated the changes in sugar content and sugar-metabolizing enzymes in different sections of pitaya fruit during various developmental phases. Genes related to sugar metabolism were screened using transcriptome analysis. The results revealed that sugar accumulation varied in different sections of pitaya fruit, and vacuolar acid invertase and sucrose synthase played crucial roles in sugar metabolism. HpVAI1 was found to have a key regulatory role in fruit development. These findings provide important information for improving fruit quality and breeding new pitaya cultivars.
HORTICULTURAL PLANT JOURNAL
(2022)
Article
Horticulture
Jiaxuan Chen, Yuanju Yuan, Fangfang Xie, Zhike Zhang, Jianye Chen, Rong Zhang, Jietang Zhao, Guibing Hu, Yonghua Qin
Summary: This study analyzed the changes in organic acid content and composition, as well as the expression patterns of key genes related to organic acid metabolism, during fruit maturation of different pitaya cultivars. The results showed that the organic acid content and composition varied among the different cultivars. Citramalate synthase played an important role in the synthesis of citramalic acid, and different HuIPMS genes exhibited differential expression levels in different parts of the pitaya fruit. These findings provide important insights into the molecular mechanism of citramalic acid metabolism during pitaya fruit maturation.
