Journal
SCIENTIFIC REPORTS
Volume 7, Issue -, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41598-017-02009-3
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Funding
- Health Department of Western Australia [RTP 9]
- Bill and Melinda Gates Foundation (Gates Foundation Grand Challenges) [OPP 1150984]
- Accelerate Diagnostics
- Achaogen Inc.
- Allecra Therapeutics
- Amplex
- AstraZeneca UK Ltd.
- Basilea Pharmaceutica
- Becton Dickinson Diagnostics
- BioMerieux
- Bio-Rad Laboratories
- BSAC
- Cepheid
- Check-Points B.V.
- Cubist Pharmaceuticals
- Department of Health
- Enigma Diagnostics
- Food Standards Agency
- GlaxoSmithKline Services Ltd.
- Henry Stewart Talks
- IHMA Ltd.
- Kalidex Pharmaceuticals
- Melinta Therapeutics
- Merck Sharpe Dohme Corp
- Meiji Seika Pharma Co
- Mobidiag
- Momentum Biosciences Ltd.
- Nordic Pharma Ltd.
- Norgine Pharmaceuticals
- Rempex Pharmaceuticals Ltd.
- Roche
- Rokitan Ltd.
- Smith Nephew UK Ltd.
- Trius Therapeutics
- VenatoRx Pharmaceuticals and Wockhardt Ltd.
- University Government
- State Government
- Commonwealth Government
- Bill & Melinda Gates Foundation - Grand Challenges Explorations Initiative [OPP1150984] Funding Source: researchfish
- Bill and Melinda Gates Foundation [OPP1150984] Funding Source: Bill and Melinda Gates Foundation
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The expanding global distribution of multi-resistant Klebsiella pneumoniae demands faster antimicrobial susceptibility testing (AST) to guide antibiotic treatment. Current ASTs rely on time-consuming differentiation of resistance and susceptibility after initial isolation of bacteria from a clinical specimen. Here we describe a flow cytometry workflow to determine carbapenem susceptibility from bacterial cell characteristics in an international K. pneumoniae isolate collection (n = 48), with a range of carbapenemases. Our flow cytometry-assisted susceptibility test (FAST) method combines rapid qualitative susceptible/non-susceptible classification and quantitative MIC measurement in a single process completed shortly after receipt of a primary isolate (54 and 158 minutes respectively). The qualitative FAST results and FAST-derived MIC (MICFAST) correspond closely with broth microdilution MIC (MICBMD, Matthew's correlation coefficient 0.887), align with the international AST standard (ISO 200776-1; 2006) and could be used for rapid determination of antimicrobial susceptibility in a wider range of Gram negative and Gram positive bacteria.
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