Journal
SCIENTIFIC REPORTS
Volume 7, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41598-017-01454-4
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Funding
- Ministere de l'Enseignement Superieur et de la Recherche
- Universite Paris-Descartes
- Centre National de la Recherche Scientifique (CNRS)
- Institut National de la Sante et de la Recherche Medicale (INSERM)
- Agence Nationale de la Recherche (ANR Chipset) [ANR-15-CE11-0008-04]
- SIRIC CARPEM
- Region Ile-de-France
- ITMO National Cancer Alliance for Life Sciences and Health (AVIESAN, INSERM) [HAP_2012001]
- Association pour la Recherche sur le Cancer for fellowships within the Frontiers in Life Science Ph.D. program (FdV)
- INSERM Physicancer program [PC201423]
- ERC [306385-SofI]
- French state [ANR-10-IDEX-03-02]
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Droplet-based microfluidics is extensively and increasingly used for high-throughput single-cell studies. However, the accuracy of the cell counting method directly impacts the robustness of such studies. We describe here a simple and precise method to accurately count a large number of adherent and non-adherent human cells as well as bacteria. Our microfluidic hemocytometer provides statistically relevant data on large populations of cells at a high-throughput, used to characterize cell encapsulation and cell viability during incubation in droplets.
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