Article
Horticulture
Chenjie Zhang, Chengwei Song, Linfeng Chen, Huili Ma, Yabing Zhang, Dalong Guo, Lili Guo, Xiaogai Hou
Summary: The aim of this study was to identify suitable reference genes for normalizing the expression levels of miRNA in different developmental stages and tissues. Five algorithms were used for stability analysis, and the results identified mtr-MIR160b-p3 and gma-miR394a-5p as the most stable miRNAs during bud development in early-flowering tree peony 'Feng dan', while PC-5p-19095 was the most stable during bud development in late-flowering tree peony 'Lian he'. Additionally, mtr-miR159a was found to be the most stable miRNA during flower development stages of different tree peony varieties.
Article
Biotechnology & Applied Microbiology
Jeongah Song, Jeonghee Cho, Jeongsik Park, Jeong Ho Hwang
Summary: This study identified stable reference genes in seven tissues across four developmental stages in minipigs, which can be used to evaluate mRNA expression levels. HPRT1 and 18S were found to be the most stable genes. The expression levels of ACE2 in the intestine and kidney increased significantly at PND28, consistent with the ACE2 expression pattern in humans.
Article
Multidisciplinary Sciences
Young-Mi Lee, Hayoung Cho, Ryeo-Ok Kim, Soyeon In, Se-Joo Kim, Eun-Ji Won
Summary: Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) is a primary method for evaluating gene expression, but the stability of reference genes in small invertebrates like Diaphanosoma celebensis under different ages and exposure conditions is limited. This study evaluated the expression profiles of nine candidate reference genes under diverse toxicant exposure and growth conditions, resulting in similar gene stabilities under chemical exposures but significantly different patterns based on age, impacting the expression of development and detoxification-related genes. This research is a crucial step towards standardized qRT-PCR analysis for ecotoxicology studies involving environmentally important invertebrates like D. celebensis.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Mengdi Chen, Zhengbo Wang, Ziyuan Hao, Hongying Li, Qi Feng, Xue Yang, Xiaojiao Han, Xiping Zhao
Summary: This study selected suitable internal reference genes for studying gene expression in Broussonetia papyrifera under abiotic stresses. Through RT-qPCR and stability analysis, the best internal reference genes and their combinations were determined for different stress conditions and tissues. The reliability of the screened internal reference genes was further verified by analyzing the expression patterns of target genes. This study lays the foundation for functional analysis and regulatory mechanism research of genes in B. papyrifera.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Zoology
Mengke Ni, Zhichao Li, Jing Li, Hui He, Yaling Wang, Yixuan Jiang, Xianwei Wang, Zhuanjian Li, Ming Li, Huifen Xu
Summary: This study aimed to screen the most stable reference genes in different muscle tissues of New Zealand white rabbits and Yufeng yellow rabbits. The results showed that the most stable reference genes varied among different muscle tissues and different breeds of rabbits, but HPRT1, PPIC, and SDHA had high stability and could be used as the best reference for RT-qPCR in muscle tissues.
Article
Plant Sciences
Rohit Bharati, Madhab Kumar Sen, Ram Kumar, Aayushi Gupta, Vishma Pratap Sur, Ingrid Melnikovova, Eloy Fernandez-Cusimamani
Summary: This study evaluated the stability of seven commonly used reference genes in rosemary under different elicitor and stress conditions using RT-qPCR. The combination of F1-ATPase, ATP synthase, and ACCase was recommended for normalizing gene expression experiments in rosemary under in vitro conditions. In addition, reference genes for several plant tissues, elicitors, and stresses were proposed.
Article
Agronomy
Junliang Yin, Lu Hou, Xinchen Jiang, Jie Yang, Yang He, Xiaokang Zhou, Xiongmeng Zhu, Andong Gong, Yongxing Zhu, Zhongyi Chen
Summary: Alligatorweed is an invasive semiaquatic weed that poses a serious threat to agricultural production and ecological balance. This study identified stable reference genes for gene expression analysis in A. philoxeroides, laying a foundation for further research on gene expression profiling and invasive weed management.
Article
Multidisciplinary Sciences
Hao Zhan, Hanzhang Liu, Tianchong Wang, Lin Liu, Wanfeng Ai, Xiujun Lu
Summary: This study selected 11 candidate reference genes and evaluated their expression stability in different tissues and under various stress conditions in Quercus mongolica. The results showed that the most stable genes varied in different experimental environments. Moreover, the study demonstrated the reliability of the selected reference genes in different tissues and under different stress conditions.
Article
Veterinary Sciences
Le Zhao, Haili Yang, Xingchun Li, Yumei Zhou, Taolu Liu, Yongju Zhao
Summary: This study evaluated candidate reference genes for the early whiting mechanism of goat brown adipose tissue (BAT) and identified the most stable reference genes. These findings contribute to further understanding the molecular mechanisms underlying BAT whiting in goats and provide important reference genes for research in this field.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Physiology
Jinrong Tang, Gemei Liang, Shaoqi Dong, Shuang Shan, Man Zhao, Xianru Guo
Summary: By evaluating the stability of reference genes in Athetis dissimilis under different conditions, suitable reference genes for different experimental conditions can be determined. Different reference genes are needed under different experimental conditions, providing a reliable basis for studying gene expression in A. dissimilis.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Yang Hu, Qi Jiang, Xiang Zhai, Liang Liu, Yuntian Hong
Summary: This study screened and verified the optimal panel of reference genes in colorectal cancer and normal colonic cell lines. The results showed that the expression levels of candidate reference genes varied in different cell lines, but the optimal panel of reference genes always consisted of two genes. Moreover, the optimal combination of reference genes varied among different cell lines.
SCIENTIFIC REPORTS
(2023)
Article
Genetics & Heredity
Hua Cao, Han Li, Lin Lu, Yulu Ji, Lulin Ma, Shenchong Li
Summary: This study selected and evaluated the suitability of ten candidate RGs for normalizing leaf color-related genes' expression levels via qRT-PCR. The stability rankings analysis disclosed that EF1 alpha exhibited the highest stability and was chosen as the most reliable reference gene. The results offer key genetic resources for the functional characterization of leaf color-related genes and will pave the way for molecular dissection of leaf color mutations in D. officinale.
Article
Biochemical Research Methods
Yunfang Tian, Zhigang Chu, Linqing Wang, Huiyu Wang, Xiuyun Yuan, Si Wu, Yuzhen Yang
Summary: In this study, reference genes for different organs and stages of Cymbidium faberi were identified through experimental detection. Different combinations of reference genes were determined for different stages. The results showed that the expression of CfAG1 gene was more stable when UBQ3, GAPDH, and ACT were used as reference genes.
Article
Biochemical Research Methods
Yunfang Tian, Zhigang Chu, Huiyu Wang, Guoxia Wang, Si Wu, Yuzhen Yang
Summary: Selection of reference genes is crucial for accurate analysis of RT-qPCR results. This study identified the most stable reference genes for Cymbidium sinense, with ACT and EF1 alpha being the top choices for all tissues. The expression of the target gene AG was successfully verified using EF1 alpha, ACT, GAPDH, UBQ2, and UBQ3 as reference genes. The findings have practical implications for studying key gene expression in C. sinense development.
Article
Plant Sciences
Rohit Bharati, Madhab Kumar Sen, Ram Kumar, Aayushi Gupta, Jana Ziarovska, Eloy Fernandez-Cusimamani, Olga Leuner
Summary: This study assessed the suitability of ten commonly used reference genes as optimal reference genes in M. officinalis under different abiotic stress conditions and plant organs. The combination of EF-1 alpha and GAPDH was recommended as the best reference genes for gene expression studies in M. officinalis.
Editorial Material
Cardiac & Cardiovascular Systems
Merry L. Lindsey, Zamaneh Kassiri, Kara Hansell Keehan, Keith R. Brunt, Jason R. Carter, Jonathan A. Kirk, Petra Kleinbongard, Amanda J. LeBlanc, Crystal M. Ripplinger
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Article
Infectious Diseases
Jose Alejandro Bohorquez, Enrica Sozzi, Miaomiao Wang, Monica Alberch, Xavier Abad, Alessandra Gaffuri, Davide Lelli, Rosa Rosell, Lester Josue Perez, Ana Moreno, Llilianne Ganges
Summary: Several emerging pestiviruses, including a new ovine pestivirus (OVPV) highly genetically similar to classical swine fever virus (CSFV), have been reported to cause disease recently. OVPV infection in piglets resulted in only mild clinical signs but induced early interferon-alpha levels and cross-neutralizing antibodies against CSFV. Close antigenic relation and robust protection against CSFV suggest possible coevolution of OVPV and CSFV as two branches from a shared origin in different hosts.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Editorial Material
Cardiac & Cardiovascular Systems
Victoria L. Nelson, Ashley L. Eadie, Keith R. Brunt
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2022)
Article
Immunology
Karl A. Ciuoderis, Michael G. Berg, Lester J. Perez, Abbas Hadji, Laura S. Perez-Restrepo, Leidi Carvajal Aristizabal, Kenn Forberg, Julie Yamaguchi, Andres Cardona, Sonja Weiss, Xiaoxing Qiu, Juan Pablo Hernandez-Ortiz, Francisco Averhoff, Gavin A. Cloherty, Jorge E. Osorio
Summary: Arbovirus infections, including dengue and Oropouche virus, are common causes of acute febrile illness in tropical countries like Colombia. This study conducted surveillance and found Oropouche virus to be an emerging pathogen. The results highlight the need for increased monitoring to assess the burden of Oropouche virus as a cause of acute febrile illness in Colombia.
EMERGING MICROBES & INFECTIONS
(2022)
Review
Cardiac & Cardiovascular Systems
Razoan Al Rimon, Victoria L. Nelson, Keith R. Brunt, Zamaneh Kassiri
Summary: This article discusses the multifaceted nature of myocardial ischemic injury, highlighting recent state-of-the-art findings and strategies that provide new directions for high-impact research on myocardial tissue remodeling.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2022)
Editorial Material
Cardiac & Cardiovascular Systems
Merry L. Lindsey, Jason R. Carter, Crystal M. Ripplinger, Zamaneh Kassiri, Kara Hansell Keehan, Keith R. Brunt, Jonathan A. Kirk, Petra Kleinbongard, Amanda J. LeBlanc
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
B. Coyle-Asbil, E. J. B. Holjak, J. P. Marrow, R. Alshamali, L. M. Ogilvie, B. A. Edgett, L. D. Hopkinson, K. R. Brunt, J. A. Simpson
Summary: Cardiac reserve is a widely used health indicator and prognostic tool, but assessing it in preclinical models is challenging. There is less focus on diastolic reserve compared to systolic reserve. This study aimed to determine the techniques and parameters for assessing systolic and diastolic reserves in preclinical models. The findings showed that systolic reserve can be assessed using all techniques, but current methods are ineffective for evaluating diastolic reserve in healthy mice in vivo.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Iryna Savinova, Alexandra Fournier, Stacy Grieve, Keith R. Brunt
Summary: This summary discusses the results of a study on blood cancer detection. The researchers used a new tool called Raman spectroscopy to detect unique molecules in the blood plasma of people with or recovering from blood cancer. The study found that this tool can improve the accuracy of blood cancer detection and monitoring the progress of recovery or relapse.
Editorial Material
Cardiac & Cardiovascular Systems
Merry L. Lindsey, Zamaneh Kassiri, Amanda J. LeBlanc, Crystal M. Ripplinger, Jonathan A. Kirk, Jason R. Carter, Petra Kleinbongard, Keith R. Brunt
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2023)
Editorial Material
Cardiac & Cardiovascular Systems
Merry L. Lindsey, Petra Kleinbongard, Zamaneh Kassiri, Jason R. Carter, Kara Hansell Keehan, Crystal M. Ripplinger, Amanda J. LeBlanc, Keith R. Brunt, Jonathan A. Kirk
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Chenxuan Wang, Barbora Hucik, Ousseynou Sarr, Liam H. Brown, Kyle R. D. Wells, Keith R. Brunt, Manabu T. Nakamura, Ewa Harasim-Symbor, Adrian Chabowski, David M. Mutch
Summary: The study aimed to investigate the impact of a D6D deficiency on triacylglycerol storage and lipolytic and lipogenic pathways in white adipose tissue. The results showed that a D6D deficiency alters fatty acid cycling in adipose tissue by promoting lipolysis and reducing fatty acid re-esterification.
JOURNAL OF LIPID RESEARCH
(2023)
Article
Microbiology
Gregory S. Orf, Lester J. Perez, Karl Ciuoderis, Andres Cardona, Simon Villegas, Juan P. Hernandez-Ortiz, Guy Baele, Aurash Mohaimani, Jorge E. Osorio, Michael G. Berg, Gavin A. Cloherty
Summary: The first 18 months of SARS-CoV-2 infections in Colombia experienced three epidemic waves. During the third wave, the Mu variant replaced Alpha and Gamma through intervariant competition. Phylogeographic analysis showed that Mu acquired increased fitness in Colombia and spread to North America and Europe. Despite not being the most transmissible, Mu's genetic composition and ability to evade preexisting immunity allowed it to dominate the Colombian epidemic landscape.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Lester J. Perez, Gavin A. Cloherty, Michael G. Berg
Summary: Picobirnaviruses (PBVs) are small double-segmented, double-stranded RNA viruses that are frequently associated with gastrointestinal and respiratory infections. The evolutionary analysis revealed that PBVs emerged simultaneously from avian Reovirus and fungal Partitivirus ancestors, indicating parallel evolution from different hosts has driven the genetic similarities among PBV species. These findings provide insights into the origins and genetic diversification of the Picobirnaviridae family.
MICROBIOLOGY SPECTRUM
(2023)
Article
Virology
Gregory S. Orf, Lester J. Perez, Todd Meyer, Ka-Cheung Luk, Kenn Forberg, Mary A. Rodgers, Abbas Hadji, Linda James, Samuel Mampunza, Asmeeta Achari, Guixia Yu, Scot Federman, Charles Y. Chiu, Carole A. McArthur, Gavin A. Cloherty, Michael G. Berg
Article
Virology
Lester J. Perez, Gregory S. Orf, Michael G. Berg, Mary A. Rodgers, Todd Meyer, Aurash Mohaimani, Ana Olivo, Barbara Harris, Illya Mowerman, Abdou Padane, Agbogbenkou Tevi Dela-del Lawson, Aminata Mboup, Moustapha Mbow, Nafissatou Leye, Ndeye Coumba Toure-Kane, Ambroise D. Ahouidi, Gavin A. Cloherty, Souleymane Mboup
Summary: Molecular surveillance of SARS-CoV-2 in Senegal reveals two waves of infection, with the B.1.416 lineage originating in Senegal and spreading mainly to Europe, and the B.1.1.420 lineage introduced from Italy and gaining fitness in Senegal before spreading globally. These lineages carry selected mutations that may explain their local dominance, emphasizing the importance of monitoring their mutation profiles. Molecular surveillance in Africa will play a vital role in controlling the spread of the pandemic.