Article
Biochemistry & Molecular Biology
Uylae Kim, Elizabeth S. Paul, Arturo Diaz
Summary: Microbacterium foliorum-derived lytic bacteriophages, including YuuY, have been characterized in this study. These phages possess broad host range and high temperature tolerance, making them highly valuable for further research and potential applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Virology
Katrine Wacenius Skov Alanin, Nikoline S. S. Olsen, Amaru Miranda Djurhuus, Alexander Byth Carstens, Tue Kjaergaard Nielsen, Natalia Wagner, Rene Lametsch, Frederik Bak, Rosanna Catherine Hennessy, Mette Haubjerg Nicolaisen, Witold Kot, Lars Hestbjerg Hansen
Summary: Despite the importance of viral communities, phages are not well-studied. Current genomic databases lack high-quality phage genome sequences linked to specific bacteria. In this study, three Erwinia billingiae phages were isolated from organic household waste. Based on sequence similarity, they are proposed to represent three new genera within different viral families, highlighting the need for further exploration in this field.
ARCHIVES OF VIROLOGY
(2023)
Article
Microbiology
Bibiana Martins Barasuol, Juliana Felipetto Cargnelutti, Luis Antonio Sangioni, Daniela Isabel Brayer Pereira, Ana Paula Muterle Varela, Fabiana Quoos Mayer, Emanuele Serro Pottker, Gabriela Flores Goncalves, Samuel Cibulski, Sonia de Avila Botton
Summary: In this study, novel S. aureus temperate phages were isolated and characterized from bovine milk, showing lytic activity and phylogenic similarity with other Staphylococcus phages. These phages belong to the family Siphoviridae but are distinct from other species within the family.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Virology
Jean Pierre Gonzalez-Gomez, Osvaldo Lopez-Cuevas, Nohelia Castro-del Campo, Irvin Gonzalez-Lopez, Celida Isabel Martinez-Rodriguez, Bruno Gomez-Gil, Cristobal Chaidez
Summary: Two lytic Vibrio phages (vB_VpaP_AL-1 and vB_VpaS_AL-2) isolated from estuary water in Sinaloa, Mexico were found to be potential biocontrol agents against AHPND-causing V. parahaemolyticus.
Article
Chemistry, Multidisciplinary
Urszula Wydro, Magdalena Jankowska, Elzbieta Wolejko, Pawel Kondzior, Bozena Lozowicka, Piotr Kaczynski, Joanna Rodziewicz, Wojciech Janczukowicz, Anna Pietryczuk, Adam Cudowski, Agata Jablonska-Trypuc
Summary: Sewage sludge, as an organic substrate, can improve soil quality exposed to pesticides by providing essential nutrients and organic matter. This study investigated the influence of granulated sewage sludge on soil biological properties treated with herbicides and fungicides. Results showed that the application of sewage sludge influenced soil enzymatic activity, genetic diversity of microorganisms, and the abundance of specific bacterial gene.
APPLIED SCIENCES-BASEL
(2022)
Article
Immunology
R. Preenanka, Muhammed P. Safeena
Summary: This study aimed to isolate and purify obligately lytic bacteriophages and study its morphological, genetic and biological characteristics. The four bacteriophages isolated in this study exhibit 100% infectivity to S. agalactiae serotype Ia, a predominate serotype infecting fish. The phages were found to be stable and potential to eliminate the S. agalactiae serotype Ia infecting fish.
MICROBIAL PATHOGENESIS
(2023)
Article
Engineering, Environmental
Katherine E. Graham, Stephanie K. Loeb, Marlene K. Wolfe, David Catoe, Nasa Sinnott-Armstrong, Sooyeol Kim, Kevan M. Yamahara, Lauren M. Sassoubre, Lorelay M. Mendoza Grijalva, Laura Roldan-Hernandez, Kathryn Langenfeld, Krista R. Wigginton, Alexandria B. Boehm
Summary: The study indicates that measuring SARS-CoV-2 RNA concentrations in settled solids may be a more sensitive approach than measuring SARS-CoV-2 in influent, providing important information for public health response to viral diseases like COVID-19.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Virology
Martyna Cieslik, Marek Harhala, Filip Orwat, Krystyna Dabrowska, Andrzej Gorski, Ewa Jonczyk-Matysiak
Summary: In this study, two newly isolated myoviruses that can lyse multidrug-resistant Enterobacter spp. were characterized. These phages belong to a poorly represented phage group and show potential for therapeutic use against urinary tract infections.
Article
Genetics & Heredity
Julia Hankel, Sophie Kittler, Bussarakam Chuppava, Eric Galvez, Till Strowig, Andre Becker, Maren von Koeckritz Blickwede, Madeleine Plotz, Christian Visscher
Summary: This study aimed to investigate the interactions between the host microbiota and Campylobacter jejuni in broiler chickens. The results showed that the infection status of C. jejuni significantly influenced the microbial composition in the chicken's intestines, with the presence of certain bacterial families being associated with C. jejuni infection. The Christensenellaceae R-7 group was found to have a negative correlation with the colonization of C. jejuni.
MICROBIAL GENOMICS
(2022)
Review
Chemistry, Multidisciplinary
Sophie Joimel, Antonin Jules, Laure Vieuble Gonod
Summary: This article reviews 75 articles on soil collembola in an urban context, finding that soil properties and landscape characteristics influence soil communities. There is a need for more connectivity between green urban spaces to allow the dispersion of collembola.
ENVIRONMENTAL CHEMISTRY LETTERS
(2022)
Article
Microbiology
Greg P. Krukonis, Amanda K. Kemp, Katie F. Storrie, Vivian R. Chavira, Hayden W. Lantrip, Victoria D. Perez, Desiree A. Reyes, Julian A. Truax, Rachel Loney, Veronique A. Delesalle
Summary: Bacteriophages play a crucial role in the structure of bacterial communities, particularly in desert soils dominated by Bacillus species. In this study, we report the isolation of two genetically similar temperate phages from a Bacillus subtilis strain in soil from Tucson, Arizona. These phages have double-stranded DNA genomes with 98 and 102 genes, with four genes being unique to one phage.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Article
Immunology
Patrick D. Mathews, Marcus Domingues, Antonio A. M. Maia, Marcia R. M. Silva, Edson A. Adriano, Julio C. Aguiar
Summary: In this study, a new dactylogyrid species, Ameloblastella pirarara sp. n., is described from the gills of an Amazonian catfish. It is distinguished from its congeners by morphological features and phylogenetic analysis, expanding our knowledge of monogenoid parasites from Amazonian freshwater fish.
MICROBIAL PATHOGENESIS
(2021)
Review
Biochemistry & Molecular Biology
Fauzia Izzati, Mega Ferdina Warsito, Asep Bayu, Anggia Prasetyoputri, Akhirta Atikana, Linda Sukmarini, Siti Irma Rahmawati, Masteria Yunovilsa Putra
Summary: Marine invertebrates from Indonesia are reported to have a high chemical diversity and broad spectrum of biological activities. This review summarizes the exploration of bioactive compounds from Indonesian marine invertebrates between 2007-2020, highlighting their antimicrobial, antifungal, anticancer, and antiviral properties, and suggests further investigation of novel compounds.
Article
Biodiversity Conservation
Joudellys Andrade-Silva, Fabricio B. Baccaro, Livia P. Prado, Benoit Guenard, Dan L. Warren, Jamie M. Kass, Evan P. Economo, Rogerio R. Silva
Summary: This study introduces and analyzes a comprehensive data compilation of Amazonian ant diversity, providing insights into the distribution and diversity of ant species in the Brazilian Amazon Basin. The results show that ant sampling locations are influenced by access routes, urban centers, research institutions, and infrastructure projects. Distance to roads is found to be the main driver of ant sampling in the Amazon. Contrary to expectations, morphological traits have lower predictive power in predicting sampling bias compared to purely geographic variables.
Article
Geography, Physical
Bruno Carvalho Carneiro de Mendonca, Luca Mao, Barbara Belletti
Summary: Rivers play a crucial role in shaping landscapes and connecting various organisms. Human disturbances often require river restoration to enhance ecological diversity and ecosystem services. The relationship between physical diversity of rivers and biological communities is not always straightforward due to complex interactions.
Article
Genetics & Heredity
Zuzanna Cyske, Lidia Gaffke, Karolina Pierzynowska, Grzegorz Wegrzyn
Summary: This report demonstrates, for the first time, the changes in the levels of long non-coding RNAs (lncRNAs) in fibroblasts derived from patients with mucopolysaccharidosis (MPS). Certain lncRNAs (SNHG5, LINC01705, LINC00856, CYTOR, MEG3, and GAS5) were found to be significantly elevated in several types of MPS. Potential target genes for these lncRNAs were identified, and correlations between the altered lncRNA levels and changes in mRNA transcripts of specific genes were observed. The findings suggest that lncRNA levels play a significant role in the pathomechanism of MPS.
Review
Microbiology
Karolina Pierzynowska, Joanna Morcinek-Orlowska, Lidia Gaffke, Weronika Jaroszewicz, Piotr M. Skowron, Grzegorz Wegrzyn
Summary: The phage display technology utilizes bacteriophages to present a wide variety of peptides, leading to significant advancements in the selection of bioactive molecules. It has been extensively applied in various fields such as immunology, biomedicine, and material research. This comprehensive overview of the potential applications of phage display technology highlights its significance in science, medicine, and biotechnology.
CRITICAL REVIEWS IN MICROBIOLOGY
(2023)
Article
Cell Biology
Maria Szrejder, Marlena Typiak, Piotr Pikul, Irena Audzeyenka, Patrycja Rachubik, Dorota Rogacka, Magdalena Narajczyk, Agnieszka Piwkowska
Summary: Lactate has been found to be a fuel energy source and plays an essential role in podocyte metabolism. The study demonstrated the presence of lactate transporters in podocytes and observed changes in their amount in response to glucose availability. Lactate exposure preserved cell survival and prevented glycogen reserves depletion during glucose deprivation.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Karolina Pierzynowska, Lidia Gaffke, Magdalena Zabinska, Zuzanna Cyske, Estera Rintz, Karolina Wisniewska, Magdalena Podlacha, Grzegorz Wegrzyn
Summary: The oxytocin receptor (OXTR) is responsible for signal transduction after binding with oxytocin, and it plays a role in both maternal behavior and nervous system development. Dysfunctions and polymorphisms in OXTR can lead to various diseases, including mental disorders and reproductive organ problems. Recent studies also show that changes in OXTR levels and aggregation can affect the development of inherited metabolic diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Miroslawa Panasiuk, Milena Chraniuk, Karolina Zimmer, Lilit Hovhannisyan, Vasil Krapchev, Grazyna Peszynska-Sularz, Magdalena Narajczyk, Jan Weslawski, Agnieszka Konopacka, Beata Gromadzka
Summary: Chimeric virus-like particles (cVLPs) have the potential to improve public health as safe and effective vaccine candidates. Capsid proteins of caliciviruses can be genetically modified to present foreign epitopes on the surface of cVLPs, enhancing the immune response to low immunogenic antigens. The impact of epitope localization in different structural loops of the VP1 protein of norovirus (NoV) on immunogenicity was investigated using insect cells to produce cVLPs presenting influenza virus-conserved epitopes. The study compared the specific reaction to influenza epitopes in vaccinated mice to determine the impact of epitope localization on immunogenicity.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Magdalena Zabinska, Lidia Gaffke, Patrycja Bielanska, Magdalena Podlacha, Estera Rintz, Zuzanna Cyske, Grzegorz Wegrzyn, Karolina Pierzynowska
Summary: Mucopolysaccharidoses (MPS) are rare genetic disorders characterized by the accumulation of glycosaminoglycans (GAGs) in lysosomes due to mutations in lysosomal enzymes. This study found significantly reduced levels of chaperone proteins Hsp70 and Hsp40 in MPS cells. Lowering GAG levels did not normalize chaperone levels, suggesting that reduced chaperone levels may contribute to the low activity of lysosomal enzymes in MPS.
Review
Biochemistry & Molecular Biology
Karolina Pierzynowska, Lidia Gaffke, Jan M. Zaucha, Grzegorz Wegrzyn
Summary: CAR-T cells are genetically modified T cells that can detect and eliminate cancer cells. While it provides new hope for cancer patients, there are challenges such as toxicity and limited applicability. Transcriptomics, a technique analyzing RNA abundance, is used to study and improve CAR-T cells in terms of efficacy, toxicity reduction, targeting solid tumors, treatment monitoring, and method development.
Article
Chemistry, Analytical
Katarzyna Szot-Karpinska, Patryk Kudla, Urszula Orzel, Magdalena Narajczyk, Martin Jonsson-Niedziolka, Barbara Palys, Slawomir Filipek, Andreas Ebner, Joanna Niedziolka-Jonsson
Summary: This study investigates the interactions between C-reactive protein (CRP) and CRP-binding peptide materials, identifying the best binder as the P3 peptide. The P3 peptide is used for CRP recognition on electrodes, demonstrating its potential as a receptor for CRP.
ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Adrian Koterwa, Mattia Pierpaoli, Bozena Nejman-Falenczyk, Sylwia Bloch, Artur Zielinski, Wioletta Adamus-Bialek, Zofia Jeleniewska, Bartosz Trzaskowski, Robert Bogdanowicz, Grzegorz Wegrzyn, Pawel Niedzialkowski, Jacek Ryl
Summary: This manuscript presents a novel approach using multiparametric impedance discriminant analysis (MIDA) to address the challenges of electrode fouling and highly complex electrode nanoarchitecture in biosensors operating in real environments. Real-time monitoring combined with singular value decomposition and partial least squares discriminant analysis enables selective identification of the analyte from raw impedance data without the use of electric equivalent circuits. The proposed approach offers a limit of detection of 11.3 CFU/mL for detecting uropathogenic Escherichia coli in real human urine.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biochemistry & Molecular Biology
Florian Turbant, Jehan Waeytens, Anais Blache, Emeline Esnouf, Vincent Raussens, Grzegorz Wegrzyn, Wafa Achouak, Frank Wien, Veronique Arluison
Summary: The carrier role of Outer Membrane Vesicles (OMVs) for small regulatory noncoding RNAs (sRNAs) has recently been demonstrated. This study shows that the protein Hfq, which is needed for the function of these sRNAs, can be translocated into the periplasm and exported in OMVs, where it may bind to sRNAs. Furthermore, Hfq interacts with and is inserted into OMV membranes, suggesting a role for this protein in the release of sRNA outside the vesicle.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Joanna Brokowska, Lidia Gaffke, Karolina Pierzynowska, Grzegorz Wegrzyn
Summary: Morquio disease, a type of lysosomal storage disease, is caused by gene mutations that lead to cellular dysfunction and accumulation of keratan sulfate. Recent studies have shown that this accumulation may also contribute to abnormal cell apoptosis, further worsening the disease. This study found evidence of apoptosis stimulation in Morquio disease cells, suggesting its involvement in the pathogenesis of the disorder.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Physiology
Alicja Braczko, Gabriela Harasim, Ada Kawecka, Iga Walczak, Malgorzata Kapusta, Magdalena Narajczyk, Klaudia Stawarska, Ryszard T. Smolenski, Barbara Kutryb-Zajac
Summary: This study analyzed the effects of atorvastatin and synthetic peptide PCSK9i on the bioenergetics and function of microvascular endothelial cells and cardiomyocytes. The results showed that PCSK9i had superior effects compared to atorvastatin.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Cell Biology
Lidia Gaffke, Estera Rintz, Karolina Pierzynowska, Grzegorz Wegrzyn
Summary: The main approach in current therapy for mucopolysaccharidosis (MPS) is to reduce glycosaminoglycan (GAG) levels in cells, which are considered the main cause of the disease. This study aimed to evaluate changes in the actin cytoskeleton and focal adhesion in cells from MPS patients and MPS I mouse model, and determine if they could be potential therapeutic targets for different MPS types. Western-blotting, flow cytometry, and transcriptomic analyses were used. Significant changes in the expression of genes involved in the actin cytoskeleton structure/functions were identified, suggesting the actin cytoskeleton as a potential therapeutic target for MPS.
Article
Cell Biology
Karolina Pierzynowska, Magdalena Podlacha, Lidia Gaffke, Estera Rintz, Karolina Wisniewska, Zuzanna Cyske, Grzegorz Wegrzyn
Summary: The study shows that genistein can stimulate autophagy in the brain of HD mice, reduce the levels of mutated HTT, improve behavior and biochemical parameters. This suggests that genistein may be considered as a potential drug for Huntington disease and indicates a direction for the development of effective therapeutic approaches.
Article
Chemistry, Analytical
Katarzyna Szot-Karpinska, Patryk Kudla, Urszula Orzel, Magdalena Narajczyk, Martin Jonsson-Niedziolka, Barbara Palys, Slawomir Filipek, Andreas Ebner, Joanna Niedziolka-Jonsson
Summary: This study investigates the interactions between C-reactive protein (CRP) and new CRP-binding peptide materials using experimental methods and theoretical simulations. Three specific CRP-binding peptides were identified and their binding efficiency towards CRP was demonstrated. The study consistently showed that the P3 peptide is the best CRP binder. The study also presents a potential candidate peptide, P3, as a receptor for CRP.
ANALYTICAL CHEMISTRY
(2023)