Article
Plant Sciences
Bernd Ackermann, Beatrix Duenschede, Bjoern Pietzenuk, Bo Hojen Justesen, Ute Kraemer, Eckhard Hofmann, Thomas Guenther Pomorski, Danja Schuenemann
Summary: Members of the Oxa1/YidC/Alb3 protein family are involved in the insertion, folding, and assembly of membrane proteins in mitochondria, bacteria, and chloroplasts. The thylakoid membrane protein Alb3 interacts with ribosomes during posttranslational insertion of specific proteins, while Alb4, a homolog located in the same membrane, does not show ribosome binding. Alb3 contacts ribosomes via its C-terminal region and at least one additional binding site within its hydrophobic core.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Cell Biology
Bettina Homberg, Peter Rehling, Luis Daniel Cruz-Zaragoza
Summary: Most mitochondrial proteins are synthesized in the cytosol and transported into mitochondria by protein translocases. The oxidase assembly (OXA) insertase in mitochondria plays a role in inserting mitochondrial-encoded proteins into the inner membrane. Recent research reveals the cooperation between OXA and the mitochondrial ribosome during the synthesis of these proteins, suggesting that OXA is a multifunctional protein insertase involved in protein transport, assembly, and stability at the inner membrane.
TRENDS IN CELL BIOLOGY
(2023)
Article
Plant Sciences
Tatiana P. Fedorchuk, Inga A. Kireeva, Vera K. Opanasenko, Vasily V. Terentyev, Natalia N. Rudenko, Maria M. Borisova-Mubarakshina, Boris N. Ivanov
Summary: Studying bicarbonate-induced stimulation of photophosphorylation in thylakoids isolated from Arabidopsis thaliana leaves revealed a potential mechanism involving alpha CA5. This mechanism was disrupted in wild-type thylakoids by a soluble carbonic anhydrase inhibitor and was also absent in mutant lines lacking alpha CA5.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Multidisciplinary Sciences
Maximilian Seidel, Natalie Romanov, Agnieszka Obarska-Kosinska, Anja Becker, Nayara Trevisan Doimo de Azevedo, Jan Provaznik, Sankarshana R. Nagaraja, Jonathan J. M. Landry, Vladimir Benes, Martin Beck
Summary: Various cellular quality control mechanisms are involved in maintaining protein homeostasis. In addition to preventing misfolding during translation, ribosome-associated chaperones and importins also play roles in preventing protein aggregation and facilitating nucleocytoplasmic transport. This study suggests that importins can bind to ribosome-associated cargoes in a co-translational manner, including proteins such as ribosomal proteins, chromatin remodelers, and RNA binding proteins that are prone to cytosolic aggregation. The authors also demonstrate that importins act consecutively with other ribosome-associated chaperones, highlighting the interconnectedness of the nuclear import system and nascent chain folding.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Buesra M. Guengoer, Tamara Flohr, Sriram Garg, Johannes Herrmann, Richard Hodge
Summary: Two multisubunit protein complexes, GET complex and EMC, have been identified in the endoplasmic reticulum for membrane protein insertion. These complexes share structural similarity with a family of proteins found in bacteria, mitochondria, and chloroplasts, indicating a common ancestry. Functional studies show that these ER proteins can replace Oxa1 in yeast mitochondria, suggesting conservation of the insertion mechanism across different organelles.
Article
Multidisciplinary Sciences
Omar Sandoval-Ibanez, David Rolo, Rabea Ghandour, Alexander P. Hertle, Tegan Armarego-Marriott, Arun Sampathkumar, Reimo Zoschke, Ralph Bock
Summary: The DEIP1 protein interacts with the PetA and PetB subunits and plays an essential role in the assembly of the Cytb6f complex. This finding provides insights into the assembly pathway of a crucial complex in photosynthesis.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Michal Eisenberg-Bord, Naama Zung, Javier Collado, Layla Drwesh, Emma J. Fenech, Amir Fadel, Nili Dezorella, Yury S. Bykov, Doron Rapaport, Ruben Fernandez-Busnadiego, Maya Schuldiner
Summary: Mitochondrial functions are tightly regulated by nuclear activity, and communication between these organelles is facilitated by contact sites. In yeast, specific areas of contact between the nucleus and mitochondria were identified, with the uncharacterized protein Ybr063c (Cnm1) playing a role as a molecular tether on the nuclear membrane. Cnm1 mediates contact by interacting with Tom70 on mitochondria and is regulated by phosphatidylcholine, linking phospholipid homeostasis with contact extent.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Michal Mayer, Lulu Winer, Amihai Karniel, Elhanan Pinner, Eliane H. Yardeni, David Morgenstern, Eitan Bibi
Summary: Many integral membrane proteins are produced by translocon-associated ribosomes. Our studies reveal the co-translational targeting of FtsY and its interactions with cytosolic chaperones and the translocon, providing insights into the mechanism of membrane targeting.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Review
Plant Sciences
Akiko Yoshihara, Koichi Kobayashi
Summary: This article summarizes recent high-resolution observations of lipid molecules in the structures of photosynthetic complexes from plants, algae, and cyanobacteria, and evaluates the distribution of lipids among photosynthetic protein complexes and thylakoid lipid bilayers. By integrating the structural information with the findings from biochemical and molecular genetic studies, this article highlights the conserved and differentiated roles of lipids in the assembly and functions of photosynthetic complexes among plants, algae, and cyanobacteria.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Cell Biology
Pawel Leznicki, Hayden O. Schneider, Jada Harvey, Wei Q. Shi, Stephen High
Summary: Membrane proteins destined for lipid droplets can be delivered to the endoplasmic reticulum either co- or post-translationally. The co-translational pathway relies on the ER membrane protein complex for membrane insertion, and may result in structural rearrangements.
JOURNAL OF CELL SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Tom Joshua Eisenack, Debora Broch Trentini
Summary: Proteins are versatile molecular machines that control and execute cellular processes. They go through a multilayered synthesis process with many opportunities for error, and must navigate a complex folding-energy landscape. Newly synthesized proteins are at increased risk of misfolding and toxic aggregation, but cells employ molecular chaperones and quality control factors to maintain proteostasis.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Plant Sciences
Hui Min Olivia Oung, Roma Mukhopadhyay, Vaclav Svoboda, Dana Charuvi, Ziv Reich, Helmut Kirchhoff
Summary: The study found that in the dehydration process of the resurrection plant Craterostigma pumilum, older plants degrade specific subunits of the photosynthetic apparatus, while younger plants increase photoprotective mechanisms to maintain the ability to replace damaged subunits.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Biochemistry & Molecular Biology
Laura R. Blackholly, Nicola J. Harris, Heather E. Findlay, Paula J. Booth
Summary: The majority of alpha helical membrane proteins fold co-translationally during their synthesis on the ribosome. Cell-free translation of membrane proteins is emerging as a useful tool to address folding during translation by a ribosome. The bacterial leucine transporter, LeuT can be synthesised and inserted into lipid membranes using a variety of in vitro transcription translation systems. LeuT is considered a paradigm for neurotransmitter transporters and possesses a knotted structure that is characteristic of this transporter family.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemical Research Methods
Zhen Xiao, Chengcheng Huang, Haitao Ge, Yan Wang, Xiaoxiao Duan, Gaojie Wang, Limin Zheng, Jinghui Dong, Xiahe Huang, Yuanya Zhang, Hongyu An, Wu Xu, Yingchun Wang
Summary: Using APEX2-based proximity labeling, the study identified proteins proximal to the photosystem II oxygen evolution complex (OEC) in a cyanobacterium model. Several proteins involved in PS II assembly, maturation, and repair were discovered, along with potential regulators of PS II structure and function. This study provides valuable insights into the proteome neighborhood and regulation of a key protein complex.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Biochemistry & Molecular Biology
Vaclav Svoboda, Hui Min Olivia Oung, Haniyeh Koochak, Robert Yarbrough, Steven D. Mckenzie, Sujith Puthiyaveetil, Helmut Kirchhoff
Summary: Knowledge of the abundance and ratio of photosynthetic protein complexes in thylakoid membranes is crucial for understanding energy conversion. This paper summarizes quantitative spectroscopic and biochemical methods for quantifying plant thylakoid protein complexes. It also compares mass spectrometric and classical protein quantification methods, revealing similar quantities of complexes and confirming the potential of mass spectrometry for thylakoid protein complex quantification.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2023)
Article
Cell Biology
Aleksandra Cabaj, Adrianna Moszynska, Agata Charzynska, Rafal Bartoszewski, Michal Dabrowski
Summary: By selectively knocking down HIF-1 alpha or HIF-2 alpha, the effects of hypoxia on the transcriptional response of human umbilical endothelial cells were analyzed. It was observed that genes activated by both HIF-1 and HIF-2 tend to be activated earlier than those activated by HIF-1 alone, and the latter tend to be activated earlier than those activated by HIF-2 only.
CELLULAR SIGNALLING
(2022)
Article
Genetics & Heredity
Arkadiusz Piotrowski, Magdalena Koczkowska, Andrzej B. Poplawski, Rafal Bartoszewski, Jaroslaw Kroliczewski, Alina Mieczkowska, Alicia Gomes, Michael R. Crowley, David K. Crossman, Yunjia Chen, Ping Lao, Eduard Serra, Meritxell C. Llach, Elisabeth Castellanos, Ludwine M. Messiaen
Summary: Constitutional pathogenic variants in genes LZTR1 or SMARCB1 were identified in a high percentage of familial and sporadic schwannomatosis cases, with additional novel variants and potential predisposing candidate genes revealed through extensive sequencing.
Article
Biochemistry & Molecular Biology
Adrianna Moszynska, Maciej Jaskiewicz, Marcin Serocki, Aleksandra Cabaj, David K. Crossman, Sylwia Bartoszewska, Magdalena Gebert, Michal Dabrowski, James F. Collawn, Rafal Bartoszewski
Summary: The cellular adaptive response to hypoxia is regulated by hypoxia-inducible factors (HIFs), HIF-1 and HIF-2. miRNAs play a significant role in modulating cellular signaling pathways during the hypoxic adaptive response.
Article
Cell Biology
Marta Switalska, Beata Filip-Psurska, Magdalena Milczarek, Mateusz Psurski, Adrianna Moszynska, Aleksandra M. Dabrowska, Malgorzata Gawronska, Karol Krzyminski, Maciej Baginski, Rafal Bartoszewski, Joanna Wietrzyk
Summary: The combined treatment of C-1305 and PTX showed anticancer activity and improved tumor blood perfusion in in vivo studies. It resulted in increased level of CCL2 and decreased level of PDGF. Furthermore, the treatment modulated the levels of certain angiogenesis-related cytokines.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2022)
Review
Oncology
Sylwia Bartoszewska, James F. Collawn, Rafal Bartoszewski
Summary: The complex signaling networks and tumor microenvironments make it difficult to understand cell survival regulation in cancer. This article discusses the role of intermittent hypoxia in inducing the unfolded protein response (UPR) in the tumor microenvironment, as well as its implications for cancer progression and treatment approaches.
Article
Genetics & Heredity
Maciej Jaskiewicz, Adrianna Moszynska, Magdalena Gebert, James F. Collawn, Rafal Bartoszewski
Summary: Cellular adaptation to hypoxia is regulated by hypoxia inducible factors HIF-1 and HIF-2. HIF-1 mediates acute hypoxia response, while HIF-2 allows adaptation to chronic oxygen deprivation. Transition from HIF-1 to HIF-2 during hypoxia is possible due to the low stability of HIF-1 alpha subunit transcript (HIF1A) and the stable mRNA of HIF-2 alpha (EPAS1). However, no microRNAs (miRNAs) have been found to regulate endothelial HIF-2 levels during hypoxia.
Review
Oncology
Sylwia Bartoszewska, Jakub Slawski, James F. F. Collawn, Rafal Bartoszewski
Summary: MicroRNAs (miRNAs) play a critical role in the regulation of mRNA stability and translation. Despite our knowledge, translating miRNAs into clinical applications has been problematic. Using hsa-miR-429 as an example, the limitations in the development of efficient miRNA-related therapies and diagnostic approaches are discussed. A literature analysis of the verified hsa-miR-429 targets in various human research models is provided, and a meta-analysis is suggested for better insights into the role of hsa-miR-429 in cancer diagnosis and therapeutic approaches.
Article
Agronomy
Ewa Pecka-Kielb, Joanna Tumanowicz, Andrzej Zachwieja, Dorota Mista, Robert Kupczynski, Bozena Kroliczewska, Jowita Kaszuba, Frantisek Zigo, Tomasz Suchocki
Summary: This study aimed to analyze the changes in the profile of long-chain fatty acids in the ruminal fluid of cows during in vitro fermentation using different proportions of maize DDGS as a substrate. The use of DDGS resulted in a decrease in SFA and an increase in UFA, including oleic acid and linoleic acid. The fermentation profile with 15% and 20% DDGS proved to be the most beneficial.
Review
Agronomy
Bozena Kroliczewska, Ewa Pecka-Kielb, Jolanta Bujok
Summary: Methanogenesis is crucial in ruminant animal digestion, but the resulting methane emissions contribute significantly to greenhouse gas levels. Various strategies, including dietary modifications and feed additives, have been proposed to reduce methane emissions. However, more research is needed to validate their effectiveness and evaluate their safety and consumer acceptability.
Review
Biochemistry & Molecular Biology
Wojciech Bialek, James F. Collawn, Rafal Bartoszewski
Summary: This article discusses the role of ubiquitin-mediated protein destabilization in protein degradation, as well as the significance of the ubiquitin-independent pathway and 20S proteasome. It also explores the interaction between the ubiquitin-proteasome system and pathogenic microorganisms, and how these microorganisms manipulate the system to evade or counteract host responses.
Review
Cell Biology
Sylwia Bartoszewska, Jakub Slawski, James F. Collawn, Rafal Bartoszewski
Summary: The unfolded protein response (UPR) is a cellular mechanism that protects cells during stress conditions, and cancer cells exploit this pathway to avoid cell death. One specific pathway activated during UPR is the inositol-requiring enzyme 1a (IRE1) pathway, which is associated with cell fate decisions and has potential as a cancer therapeutic target. This article discusses different UPR signaling pathways and focuses on IRE1 inhibitors as a potential effective cancer treatment.
JOURNAL OF CELL COMMUNICATION AND SIGNALING
(2023)
Review
Biochemistry & Molecular Biology
Magdalena Gebert, Jakub Slawski, Leszek Kalinowski, James F. Collawn, Rafal Bartoszewski
Summary: Efficient brain function requires a significant amount of oxygen intake, which can lead to oxidative stress and neurodegenerative diseases. The regulation of oxygen homeostasis and redox balance in the brain is mediated by stress response pathways, including the unfolded protein response (UPR). Understanding the opposing roles of UPR signaling pathways could provide insights for preventing cognitive decline and treating neurodegenerative diseases.
Article
Oncology
Szymon Macioszek, Danuta Dudzik, Rafal Bartoszewski, Tomasz Stokowy, Diether Lambrechts, Bram Boeckx, Agnieszka Wozniak, Patrick Schoffski, Michal J. Markuszewski
Summary: In this study, integrative metabolomic and transcriptomic analyses were performed to investigate the impact of imatinib treatment on tumour biochemical processes. The results revealed differential metabolite and gene expression profiles, indicating changes in pruine and pyrinridine metabolism, butanoate metabolism, as well as alanine, aspartate, and glutamate metabolism. These findings provide insights into the mechanisms of imatinib's anti-tumour activity and suggest a potentially novel approach for enhancing its efficacy or re-sensitizing imatinib-resistant tumours.
TRANSLATIONAL ONCOLOGY
(2023)
Article
Cell Biology
Magdalena Gebert, Sylwia Bartoszewska, Lukasz Opalinski, James F. Collawn, Rafal Bartoszewski
Summary: IRE1 plays a role in miRNA regulation during ER stress. The presence of an IRE1 inhibitor significantly increases the levels of pre-miR-301a and pre-miR-106b. Additionally, miR-301a-3p targets the proapoptotic protein GADD45A. Target protection for miR-301a-3p during ER stress promotes cell death in airway epithelial cells.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Biology
Maciej Jaskiewicz, Adrianna Moszynska, Marcin Serocki, Jaroslaw Kroliczewski, Sylwia Bartoszewska, James F. Collawn, Rafal Bartoszewski
Summary: The adaptive response to hypoxia involves the accumulation of three transcription factors, HIF-1α, HIF-2α, and HIF-3α, which promote cell survival under low oxygen conditions. This study demonstrates that the HIF-3α2 isoform accumulates during late-stage hypoxia and induces the expression of the apoptosis-promoting gene DDIT4. Additionally, HIF-3α2 is expressed in multiple endothelial cell types.