Article
Cell Biology
Margarida Dantas, Andreia Oliveira, Paulo Aguiar, Helder Maiato, Jorge G. Ferreira
Summary: Mitotic entry is regulated by the activity of cyclin B1-CDK1 complexes. This study demonstrates that actomyosin-dependent nuclear tension during prophase regulates the nuclear translocation of cyclin B1 and has implications for chromosome segregation efficiency.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Itsumi Tani, Yui Oikawa, Seiyo Doi, Jose Isagani B. Janairo, Rui Kamada, Kazuyasu Sakaguchi
Summary: The nucleolus, a membrane-less structure in the cell nucleus, is involved in ribosome biogenesis through liquid-liquid phase separation (LLPS) driven by interactions between nucleolar proteins and nucleic acids. Previous studies have highlighted the role of electrostatic and cation-pi interactions between RNA and intrinsically disordered regions of nucleolar proteins in LLPS. However, the initial process of nucleolar formation, independent of RNA polymerase I, remains unclear. This study demonstrated the formation of liquid droplets by ribosomal protein uL30 and nucleolar protein nucleophosmin (NPM) in vitro without RNA. The interaction between the basic regions of uL30 and acidic regions of oligomeric NPM may underlie this liquid droplet formation. Knockdown of uL30 in cells reduced the number of nucleoli, suggesting a role for protein-protein interactions in nucleolar formation during the early stages when rRNA content is low.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Neurosciences
Zhaoyang Li, Wenhao Dong, Xinyuan Zhang, Jun-Mei Lu, Yan-Ai Mei, Changlong Hu
Summary: The study demonstrates that PKC activation inhibits Kv2.2 channel currents and alters their steady-state activation, showing that the function of Kv2.2 is dependent on its phosphorylation state. In cortical slices, PKC activation reduces the frequency of action potentials by regulating Kv2.2 channels.
NEUROSCIENCE BULLETIN
(2022)
Article
Multidisciplinary Sciences
Juliane Schiweck, Kai Murk, Julia Ledderose, Agnieszka Muenster-Wandowski, Marta Ornaghi, Imre Vida, Britta J. Eickholt
Summary: The study reveals that brain injury in mice leads to an upregulation of DBN protein in astrocytes, which is crucial for scar formation and maintenance of astrocyte reactivity. DBN-mediated membrane trafficking serves as an important neuroprotective mechanism following traumatic brain injury in mice.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Katarzyna Koltowska, Kazuhide S. Okuda, Marleen Gloger, Maria Rondon-Galeano, Elizabeth Mason, Jiachen Xuan, Stefanie Dudczig, Huijun Chen, Hannah Arnold, Renae Skoczylas, Neil Bower, Scott Paterson, Anne Karine Lagendijk, Gregory J. Baillie, Ignaty Leshchiner, Cas Simons, Kelly A. Smith, Wolfram Goessling, Joan K. Heath, Richard B. Pearson, Elaine Sanij, Stefan Schulte-Merker, Benjamin M. Hogan
Summary: The RNA helicase Ddx21 plays a crucial role in mediating Vegfc-stimulated lymphangiogenesis by controlling rDNA transcription and ribosome biogenesis in endothelial cells. It regulates cell proliferation and balances p53 function in response to Vegfc-Flt4 signaling, essential for the development of lymphatic vessels. This mechanism could be a potential target for diseases involving excessive lymphangiogenesis, such as cancer metastasis or lymphatic malformation.
NATURE CELL BIOLOGY
(2021)
Article
Dermatology
Ahmed E. I. Hamouda, Carmen Schalla, Antonio Sechi, Martin Zenke, Rebekka K. Schneider, Thomas Hieronymus
Summary: This study investigates the importance of HGF receptor/Met signaling in the migration of skin-resident Langerhans cells (LCs) and dermal dendritic cells (DCs) towards draining lymph nodes. It demonstrates that Met deficiency impairs podosome formation and gelatin degradation in DCs, leading to inefficient crossing of the basement membrane. HGF-dependent Met activation reduces adhesion and enhances motility of DCs in collagen matrices, while Met signaling has no impact on integrin-independent amoeboid migration.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2023)
Article
Multidisciplinary Sciences
Mateusz Jurga, Arwa A. Abugable, Alastair S. H. Goldman, Sherif F. El-Khamisy
Summary: R-loops, products of transcription, impact genome integrity and gene expression. Regulation of these DNA:RNA hybrids is controlled through ubiquitination-dependent mechanisms by USP11 and KEAP1, with implications for cancer and neurological diseases. Loss of USP11 leads to R-loop accumulation and double-strand breaks due to reduced SETX stability.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Wencan Zhang, Xu Cao, Xiancai Zhong, Hongmin Wu, Mingye Feng, Yousang Gwack, Isakov Noah, Zuoming Sun
Summary: This study reveals the important role of steroid nuclear receptor coactivator 2 (SRC2) in regulating the differentiation of regulatory T cells (T-regs). Deficiency of SRC2 in T cells leads to defective T-reg differentiation and the development of autoimmune phenotypes. SRC2 promotes T-reg differentiation through the regulation of Nr4a2 and Foxp3 expression.
Article
Multidisciplinary Sciences
Soohyun P. Kim, Avery H. Seward, Jean Garcia-Diaz, Nathalie Alekos, Nicole R. Gould, Susan Aja, Joseph P. Stains, Michael J. Wolfgang, Ryan C. Riddle
Summary: The nuclear receptor PPAR gamma plays a key role in metabolic function and has negative effects on skeletal homeostasis. This study suggests that the skeletal and metabolic actions of PPAR gamma are linked through the regulation of sclerostin production. Modulating sclerostin action may prevent bone loss associated with anti-diabetic therapies and enhance their metabolic actions.
Article
Biology
Sara Scinicariello, Adrian Soderholm, Markus Schaefer, Alexandra Shulkina, Irene Schwartz, Kathrin Hacker, Rebeca Gogova, Robert Kalis, Kimon Froussios, Valentina Budroni, Annika Bestehorn, Tim Clausen, Pavel Kovarik, Johannes Zuber, Gijs A. Versteeg
Summary: Tristetraprolin (TTP) stability is controlled by factors including lysine-ubiquitination and phosphorylation. The E3 ligase HUWE1 was identified as a regulator of TTP stability, indirectly controlling its phosphorylation. This study provides new insights into the regulation of TTP and its impact on immune response.
Article
Biotechnology & Applied Microbiology
Fengxia Wang, Zipeng Yu, Maolin Zhang, Mengli Wang, Xiaoduo Lu, Xia Liu, Yubin Li, Xiansheng Zhang, Bao-Cai Tan, Cuiling Li, Zhaojun Ding
Summary: The maize mutant zm66, linked to a mutation in TERMINAL EAR 1 (ZmTE1), shows shorter internodes and increased node numbers due to decreased cell elongation and disordered intercalary meristem formation. ZmTE1 regulates auxin signaling, cell division, and cell elongation. ZmWEE1 kinase phosphorylates ZmTE1 to confine it to the nucleus, while ZmPP2Ac-2 phosphatase promotes dephosphorylation and cytoplasmic localization of ZmTE1, balancing cell division and elongation for normal maize growth.
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Yu Yamaguchi, Tomoko Kadowaki, Nozomi Aibara, Kaname Ohyama, Kuniaki Okamoto, Eiko Sakai, Takayuki Tsukuba
Summary: This study identified Rab44 as a negative regulator of osteoclast differentiation and found that it interacts with Coronin1C to control cell motility in macrophages, thereby influencing osteoclast formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Katerina Wolfova, Petra Otevrelova, Ales Holoubek, Barbora Brodska
Summary: RITA activates p53 and induces apoptosis in cancer cells with wild-type p53. The study found that RITA-induced changes in NPM and NCL phosphorylation were associated with apoptosis in AML patient cells but not healthy donor cells. Different AML cell lines showed heterogeneous response to RITA, and specific phosphorylation was associated with sensitivity to RITA. The NPM pT199/pS4 ratio could be a marker for RITA treatment suitability in AML cells.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Viplov Kumar Biswas, Kaushik Sen, Abdul Ahad, Arup Ghosh, Surbhi Verma, Rashmirekha Pati, Subhasish Prusty, Sourya Prakash Nayak, Sreeparna Podder, Dhiraj Kumar, Bhawna Gupta, Sunil Kumar Raghav
Summary: Mycobacterium tuberculosis (Mtb) represses the autophagolysosome machinery to evade host-mediated killing. NCoR1 co-repressor plays a crucial role in controlling Mtb growth in myeloid cells by regulating both autophagosome maturation and lysosome biogenesis. Depletion of NCoR1 impairs the AMPK-mTOR-TFEB signaling axis, leading to dysregulated expression of proteins involved in autophagy and lysosomal biogenesis, while its rescue enhances Mtb clearance.
Article
Cell Biology
Ye-Ya Tan, Yin Zhang, Bin Li, Yang-Wen Ou, Shu-Juan Xie, Pei-Pei Chen, Shi-Qiang Mei, Qiao-Juan Huang, Ling-Ling Zheng, Liang-Hu Qu
Summary: The PERK signaling plays a crucial role in the maintenance and differentiation of myoblasts by regulating miRNA networks. Knocking down PERK leads to the transformation of C2C12 myoblasts into stem-like cells and activation of muscle differentiation-associated miRNA.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Sari Ogasawara, Yoshiro Chuman, Takahiro Michiba, Rui Kamada, Toshiaki Imagawa, Kazuyasu Sakaguchi
JOURNAL OF BIOCHEMISTRY
(2019)
Article
Multidisciplinary Sciences
Rui Kamada, Nozomi Kimura, Fumihiko Yoshimura, Keiji Tanino, Kazuyasu Sakaguchi
Article
Biochemistry & Molecular Biology
Natsumi Nakagawa, Shuya Sakaguchi, Takao Nomura, Rui Kamada, James G. Omichinski, Kazuyasu Sakaguchi
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Chemistry, Physical
Takuya Yoshida, Kazuki Yamazaki, Shunta Imai, Akinori Banno, Atsushi Kaneko, Kazuhiro Furukawa, Yoshiro Chuman
Article
Chemistry, Physical
Atsushi Kaneko, Miyuu Watari, Masataka Mizunuma, Hikaru Saito, Kazuhiro Furukawa, Yoshiro Chuman
Review
Engineering, Chemical
Masataka Mizunuma, Atsushi Kaneko, Shunta Imai, Kazuhiro Furukawa, Yoshiro Chuman
Article
Biochemistry & Molecular Biology
Mathieu Lussier-Price, Haytham M. Wahba, Xavier H. Mascle, Laurent Cappadocia, Veronique Bourdeau, Christina Gagnon, Sebastian Igelmann, Kazuyasu Sakaguchi, Gerardo Ferbeyre, James G. Omichinski
Summary: The N-terminal region of SUMO proteins plays an auto-inhibition role in promoting PML-NB formation, and this auto-inhibition can be relieved by zinc, leading to increased PML-NB formation within cells.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Rui Kamada, Sae Uno, Nozomi Kimura, Fumihiko Yoshimura, Keiji Tanino, Kazuyasu Sakaguchi
Summary: This study demonstrates the important role of PPM1D phosphatase in regulating lipid droplet (LD) formation in mature adipocytes. PPM1D controls LD size by dephosphorylating Ser511 of perilipin 1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Tatsuki Tsuruoka, Emiri Nakayama, Shingo Harashima, Takuya Endo, Rui Kamada, Kazuyasu Sakaguchi, Toshiaki Imagawa
Summary: This study reveals that the transcriptional activity of p53 protein exhibits cell-to-cell heterogeneity, which is highly dependent on the cell cycle but not seen after UV exposure. The study presents a multiplexed reporter system that allows simultaneous visualization of the transcriptional activity of p53 and cell cycle, providing a useful tool for studying biological processes involving the p53 signaling pathway.
JOURNAL OF CELL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Rui Kamada, Hiromitsu Miyazaki, Jose Isagani B. Janairo, Yoshiro Chuman, Kazuyasu Sakaguchi
Summary: Biologically derived hydrogels, such as elastin-mimetic polypeptides (EMPs), show promise for biomedical applications due to their biocompatibility, biodegradability, and low toxicity. This study successfully developed functional EMP-conjugated hydrogels that exhibit temperature-responsive swelling/shrinking properties. The swelling/shrinking capacity of the EMP hydrogels can be controlled by adjusting the amount of EMP or the salt concentration. Additionally, a smart hydrogel actuator based on EMP crosslinked hydrogels and non-crosslinked hydrogels was developed, showing bidirectional curvature behavior in response to temperature changes.
Article
Biochemistry & Molecular Biology
Shuya Sakaguchi, Natsumi Nakagawa, Haytham M. Wahba, Junya Wada, Rui Kamada, James G. Omichinski, Kazuyasu Sakaguchi
Summary: Research has shown that the tetramerization domain (p53TD) of p53 protein varies among different mammalian species, with the opossum and tree shrew p53TD being more stable than the human p53TD. The stability of p53TD is correlated with its transcription activation ability, and amino acid substitutions in specific regions of p53TD can greatly impact the hydrophobic packing of the tetramer. These findings suggest that even subtle changes in the sequence of p53TD can have significant effects on the stability and functional activity of the p53 protein.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
