4.7 Article

SNP-based high density genetic map and mapping of btwd1 dwarfing gene in barley

Journal

SCIENTIFIC REPORTS
Volume 6, Issue -, Pages -

Publisher

NATURE PORTFOLIO
DOI: 10.1038/srep31741

Keywords

-

Funding

  1. National Natural Science Foundation of China [31301310]
  2. Earmarked Fund for China Agriculture Research System [CARS-5]
  3. Fundamental Research Funds for the Central Universities [2662016PY048]

Ask authors/readers for more resources

A high-density linkage map is a valuable tool for functional genomics and breeding. A newly developed sequence-based marker technology, restriction site associated DNA (RAD) sequencing, has been proven to be powerful for the rapid discovery and genotyping of genome-wide single nucleotide polymorphism (SNP) markers and for the high-density genetic map construction. The objective of this research was to construct a high-density genetic map of barley using RAD sequencing. 1894 high-quality SNP markers were developed and mapped onto all seven chromosomes together with 68 SSR markers. These 1962 markers constituted a total genetic length of 1375.8 cM and an average of 0.7 cM between adjacent loci. The number of markers within each linkage group ranged from 209 to 396. The new recessive dwarfing gene btwd1 in Huaai 11 was mapped onto the high density linkage maps. The result showed that the btwd1 is positioned between SNP marks 7HL_6335336 and 7_249275418 with a genetic distance of 0.9 cM and 0.7 cM on chromosome 7H, respectively. The SNP-based high-density genetic map developed and the dwarfing gene btwd1 mapped in this study provide critical information for position cloning of the btwd1 gene and molecular breeding of barley.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

Article Biotechnology & Applied Microbiology

Genome architecture and diverged selection shaping pattern of genomic differentiation in wild barley

Wenying Zhang, Cong Tan, Haifei Hu, Rui Pan, Yuhui Xiao, Kai Ouyang, Gaofeng Zhou, Yong Jia, Xiao-Qi Zhang, Camilla Beate Hill, Penghao Wang, Brett Chapman, Yong Han, Le Xu, Yanhao Xu, Tefera Angessa, Hao Luo, Sharon Westcott, Darshan Sharma, Eviatar Nevo, Roberto A. Barrero, Matthew Bellgard, Tianhua He, Xiaohai Tian, Chengdao Li

Summary: Divergent selection in different environments leads to functional genomic divergence. This study examined the genomic differentiation and gene expression patterns in two populations of wild barley under abiotic stress. The researchers identified structural variants and single nucleotide polymorphisms (SNPs) that may be associated with well-differentiated phenotypes and local adaptation. The findings provide new insights into the genomic basis of local adaptation and have implications for the genetic improvement of cultivated barley.

PLANT BIOTECHNOLOGY JOURNAL (2023)

Article Agronomy

Investigate large gaps and improve genetic map in narrow-leafed lupin (Lupinus angustifolius L.)

Gaofeng Zhou, Huaan Yang, Daniel Renshaw, Chengdao Li

Summary: Genetic map is crucial for plant molecular genetic studies, serving purposes such as QTL mapping, gene cloning, and physical map construction. This study constructed a genetic map using a F-8 RIL population derived from two domesticated narrow-leafed lupin cultivars. Initially, 37 linkage subgroups were created for the 20 chromosomes, and then these subgroups were merged based on physical maps and other genetic maps. The density of DNA variations between parents strongly correlated with marker density in the genetic map.

EUPHYTICA (2023)

Article Biotechnology & Applied Microbiology

New semi-dwarfing alleles with increased coleoptile length by gene editing of gibberellin 3-oxidase 1 using CRISPR-Cas9 in barley (Hordeum vulgare L.)

Jingye Cheng, Camilla Hill, Yong Han, Tianhua He, Xingguo Ye, Sergey Shabala, Ganggang Guo, Meixue Zhou, Ke Wang, Chengdao Li

Summary: The green revolution employed genetic modification of the gibberellin hormone system to introduce dwarfing gene mutations, enabling plants to adapt to modern farming conditions. However, strong GA-related mutants had an unsatisfactory seedling emergence under drought conditions. This study introduces a semi-dwarfing gene, GA3ox1, in barley, which reduces plant height without restricting coleoptile and seedling growth. Using CRISPR/Cas9 technology, novel GA3ox1 mutants were generated and their function was validated, showing increased coleoptile length and improved seed dormancy.

PLANT BIOTECHNOLOGY JOURNAL (2023)

Article Agronomy

Positional cloning identified HvTUBULIN8 as the candidate gene for round lateral spikelet (RLS) in barley (Hordeum vulgare L.)

Dandan Qin, Gang Liu, Rui Liu, Chunchao Wang, Fuchao Xu, Qing Xu, Yu Ling, Guoqing Dong, Yanchun Peng, Shuangtao Ge, Ganggang Guo, Jing Dong, Chengdao Li

Summary: This study identifies HvTUB8 as a candidate gene for barley spike and leaf development. The mutant phenotype of round lateral spikelet and dark green leaves is caused by changes in cell arrangement in the glume and enlarged chloroplasts. RNA-Seq analysis suggests that HvTUB8 may control spike morphogenesis through ethylene homeostasis and signaling, and regulate leaf color through chlorophyll metabolism. These findings provide new insights into the mechanisms of barley development.

THEORETICAL AND APPLIED GENETICS (2023)

Article Agronomy

Pleiotropy Structures Plant Height and Seed Weight Scaling in Barley despite Long History of Domestication and Breeding Selection

Tianhua He, Tefera Tolera Angessa, Chengdao Li

Summary: Size scaling refers to the relative growth rates of different body parts in an organism, and is positively correlated. Domestication and crop breeding aim to control scaling traits in opposite directions. The genetic mechanism behind size scaling and its influence on plant height and seed weight in barley were explored using a diverse barley panel. It was discovered that pleiotropy and genetic linkage are likely responsible for the scaling of plant height and seed weight in barley. This study contributes to understanding the heritability and genetic basis of size scaling in plants.

PLANT PHENOMICS (2023)

Article Biology

Comparative gene retention analysis in barley, wild emmer, and bread wheat pangenome lines reveals factors affecting gene retention following gene duplication

Yong Jia, Mingrui Xu, Haifei Hu, Brett Chapman, Calum Watt, B. Buerte, Ning Han, Muyuan Zhu, Hongwu Bian, Chengdao Li, Zhanghui Zeng

Summary: Using genomic data from Triticeae, this study explored gene retention in the process of gene duplication, and found that gene dosage constraint and environmental adaption are associated with the retention of HPT2 gene in barley, wild emmer, and bread wheat. Comparative gene retention analysis revealed that HPT2 duplicates are mainly small-scale duplicates, experiencing relaxed selection pressure and transcriptional divergence. These duplicates are enriched with molecular-binding and defense response functions, and larger gene families are more prone to gene loss. Overall, gene dosage constraint, environmental adaption, and species-specific selection are three factors affecting gene retention after gene duplication.

BMC BIOLOGY (2023)

Article Biochemistry & Molecular Biology

Transcriptome and Metabolome Reveal the Molecular Mechanism of Barley Genotypes Underlying the Response to Low Nitrogen and Resupply

Gang Wang, Juncheng Wang, Lirong Yao, Baochun Li, Xiaole Ma, Erjing Si, Ke Yang, Chengdao Li, Xunwu Shang, Yaxiong Meng, Huajun Wang

Summary: This study investigated the mechanism of barley tolerance to low nitrogen at both the transcriptome and metabolomics levels. Significant differences were found in the transcript and metabolite levels of W26 and W20 under low nitrogen stress. Candidate genes and metabolites identified in this study will be validated in future studies.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)

Article Biochemistry & Molecular Biology

Intra-Varietal Diversity and Its Contribution to Wheat Evolution, Domestication, and Improvement in Wheat

Tianbao Li, Chuizheng Kong, Pingchuan Deng, Chengdao Li, Guangyao Zhao, Hongjie Li, Lifeng Gao, Dangqun Cui, Jizeng Jia

Summary: Crop genetic diversity is crucial for adaptation and productivity in agriculture. This study analyzed the sequence, expression, and functional diversity of homologous genes in common wheat, revealing that polyploids have more homologous diversity than diploids. The exploitation of intra-varietal diversity (IVD) might be a new approach for plant breeding, particularly for polyploid crops like wheat.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)

Review Plant Sciences

Turfgrass Salinity Stress and Tolerance-A Review

Haibo Liu, Jason L. Todd, Hong Luo

Summary: Turfgrasses are ground cover plants that have robust fibrous roots to withstand various soil stresses. Soil salinity, drought, flooding, acidity, compaction, unbalanced nutrients, heavy metals, and pollutants are major stressors for turfgrasses. Salinity is particularly significant as it is linked with drought and heat stresses. There are only a few dozen species of turfgrasses, but they vary in salinity tolerance. Improvement of turfgrass salinity tolerance has been an active area of research. This review focuses on new developments in turfgrasses for salinity stresses and provides insights for future turfgrass requirements.

PLANTS-BASEL (2023)

Article Plant Sciences

Characterization of a barley (Hordeum vulgare L.) mutant with multiple stem nodes and spikes and dwarf (msnsd) and fine-mapping of its causal gene

Dandan D. Qin, Rui Liu, Fuchao Xu, Guoqing Dong, Qing Xu, Yanchun Peng, Le Xu, Hongna Cheng, Ganggang Guo, Jing Dong, Chengdao Li

Summary: In this study, the gene HvMSNSD was identified as a key regulator of shoot branching, vegetative growth, and inflorescence development in barley. The mutant phenotype was characterized by increased stem nodes, reduced plant height, and multiple spikes on each tiller. The HvMSNSD gene controlled the phenotype through GA homeostasis and changed expression of genes related to floral development.

FRONTIERS IN PLANT SCIENCE (2023)

Article Multidisciplinary Sciences

High-quality wild barley genome assemblies and annotation with Nanopore long reads and Hi-C sequencing data

Rui Pan, Haifei Hu, Yuhui Xiao, Le Xu, Yanhao Xu, Kai Ouyang, Chengdao Li, Tianhua He, Wenying Zhang

Summary: Wild barley from Evolution Canyon in Israel's Mt. Carmel is an ideal model for studying cereal chromosome evolution. The two wild barley varieties, EC_S1 and EC_N1, come from different slopes with contrasting climates, resulting in differentiated selection. We assembled highly complete genomes for both varieties using multiple sequencing techniques. These genome assemblies will provide valuable genetic resources for domesticated barley.

SCIENTIFIC DATA (2023)

Article Plant Sciences

A Comparison of Different Stomatal Density Phenotypes of Hordeum vulgare under Varied Watering Regimes Reveals Superior Genotypes with Enhanced Drought Tolerance

Brittany Clare Robertson, Yong Han, Chengdao Li

Summary: This study investigated whether stomatal density affects the capacity of barley genotypes to resist low water availability by growing 10 barley varieties under different soil water contents. The results showed that low-stomatal-density genotypes had the least impact on biomass production during drought treatment and exhibited better wilting resistance and more heads compared to high-stomatal-density genotypes under water-deprivation conditions. A correlation analysis also revealed a strong negative linear relationship between stomatal density and head number as well as the number of days until wilting symptoms. Overall, low-stomatal-density genotypes showed promising attributes for high water-use efficiency and may be useful for future breed improvement for drought tolerance in barley.

PLANTS-BASEL (2023)

Article Biotechnology & Applied Microbiology

A pangenome analysis pipeline provides insights into functional gene identification in rice

Jian Wang, Wu Yang, Shaohong Zhang, Haifei Hu, Yuxuan Yuan, Jingfang Dong, Luo Chen, Yamei Ma, Tifeng Yang, Lian Zhou, Jiansong Chen, Bin Liu, Chengdao Li, David Edwards, Junliang Zhao

Summary: The researchers developed a novel strategy and analysis pipeline for constructing a plant pangenome and identifying the position information of genetic variants. They successfully constructed a high-quality rice pangenome and identified causal structural variations related to rice grain weight and plant height. This study provides valuable genomic resources for rice genomics research and improvement.

GENOME BIOLOGY (2023)

Article Agriculture, Multidisciplinary

Successful creation of seedless (sterile) leucaena germplasm developed from interspecific hybridisation for use as forage

D. Real, C. Revell, Y. Han, C. Li, M. Castello, C. D. Bailey

Summary: In this study, a potential seedless triploid variety of leucaena was developed through interspecific hybridization, and its feasibility was investigated through planting and evaluation at two environmentally diverse sites in Australia.

CROP & PASTURE SCIENCE (2023)

Review Biotechnology & Applied Microbiology

Unravelling inversions: Technological advances, challenges, and potential impact on crop breeding

Haifei Hu, Armin Scheben, Jian Wang, Fangping Li, Chengdao Li, David Edwards, Junliang Zhao

Summary: Inversions significantly impact plant adaptation and gene functions. This review highlights advancements in technology and methodology that allow a better understanding of inversion variants through the pangenome framework and machine learning algorithms. Genome editing is an efficient method for inducing or reversing inversion mutations in plants.

PLANT BIOTECHNOLOGY JOURNAL (2023)

No Data Available