Article
Pharmacology & Pharmacy
John D. Imig, Md Abdul Hye Khan, Anna Stavniichuk, Wojciech K. Jankiewicz, Samaneh Goorani, Michael M. Yeboah, Ashraf El-Meanawy
Summary: The incidence of ureter obstruction is increasing and recovery from this kidney injury can lead to long-term consequences such as an increased risk for salt-sensitive hypertension. A study using a RUUO model found that mice with unilateral ureteral obstruction had elevated blood pressure and decreased sodium excretion, along with renal tubular injury, inflammation, decreased vascular density, and renal fibrosis. These findings highlight the importance of addressing ureter obstruction and its potential impact on sodium excretion and hypertension.
BIOCHEMICAL PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Fayez T. Hammad, Loay Lubbad, Suhail Al-Salam, Javed Yasin, Mohamed Fizur Nagoor Meeran, Shreesh Ojha, Waheed F. Hammad
Summary: Both ureteral obstruction and hypertension have significant impacts on kidney functions. Reversible ureteral obstruction in hypertensive rats showed exaggerated alterations in renal functions and other parameters of renal injury compared to normotensive rats.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Mindi Zhao, Yameng Zhang, Jianqiang Wu, Xundou Li, Youhe Gao
Summary: Multiple sclerosis is a chronic autoimmune demyelinating disease of the central nervous system that is difficult to diagnose in early stages. A study analyzed the early urinary proteome changes in a multiple sclerosis rat model and identified potential biomarkers for early detection. Functional analysis showed that these proteins are associated with asparagine degradation, neuroinflammation, and lipid metabolism. Early treatment with methylprednisolone significantly reduced the incidence of encephalomyelitis. Urine may be a good source of biomarkers for early diagnosis of multiple sclerosis.
ROYAL SOCIETY OPEN SCIENCE
(2023)
Article
Immunology
Xiaofei Li, Jing Zhao, Said Movahedi Naini, Gianmarco Sabiu, Stefan G. Tullius, Su Ryon Shin, Jonathan S. Bromberg, Paolo Fiorina, George C. Tsokos, Reza Abdi, Vivek Kasinath
Summary: The study revealed that after unilateral ureteral obstruction in the kidney, fibroblastic reticular cells (FRCs) in the kidney-draining lymph node also become fibrogenic, contributing to fibrosis that closely mirrors changes in the kidney. Additionally, immune cell populations in the affected lymph node were significantly higher compared to the unaffected contralateral kidney-draining lymph node. This suggests a possible mechanism for fibrosis involving the TGF beta/TGF beta R signaling pathway, and future studies are needed to delve deeper into this connection between FRCs and lymph node fibrosis.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Alexis Paulina Jimenez-Uribe, Belen Bellido, Omar Emiliano Aparicio-Trejo, Edilia Tapia, Laura Gabriela Sanchez-Lozada, Jose Antonio Hernandez-Santos, Francisca Fernandez-Valverde, Estefani Yaquelin Hernandez-Cruz, Marisol Orozco-Ibarra, Jose Pedraza-Chaverri
Summary: This study evaluated the time course of mitochondrial mass and bioenergetics alterations induced by a unilateral ureteral obstruction (UUO), a widely used model to study kidney fibrosis induction and progression mechanisms. The results showed a marked reduction in mitochondrial oxidative phosphorylation (OXPHOS) and mitochondrial mass during days 7 to 28 of obstruction, suggesting a key role of mitochondrial biogenesis and mitophagy induction in the impairment of OXPHOS capacity during fibrotic progression.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Pharmacology & Pharmacy
Mei Ying Xuan, Shang Guo Piao, Jun Ding, Qi Yan Nan, Mei Hua Piao, Yu Ji Jiang, Hai Lan Zheng, Ji Zhe Jin, Can Li
Summary: Dapagliflozin alleviates renal fibrosis induced by unilateral ureteral obstruction (UUO) by inhibiting RIP1-RIP3-MLKL-mediated necroinflammation through Wnt3 alpha/beta-catenin/GSK-3 beta signaling.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Infectious Diseases
Emanuela Locci, Jiajun Liu, Gwendolyn M. Pais, Alberto Chighine, Dariusc Andrea Kahnamoei, Theodoros Xanthos, Athanasios Chalkias, Andrew Lee, Alan R. Hauser, Jack Chang, Nathaniel J. Rhodes, Ernesto d'Aloja, Marc H. Scheetz
Summary: This study investigates the potential kidney injury caused by Polymyxin B treatment and aims to detect it early through the analysis of urinary metabolites. The study found that the concentration of urinary taurine significantly increased on the first day of treatment and then returned to baseline levels. Taurine may serve as an early indicator of acute kidney damage caused by Polymyxin B.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Medicine, Research & Experimental
Stine Julie Tingskov, Michael Schou Jensen, Casper-Emil Tingskov Pedersen, Isabela Bastos Binotti Abreu de Araujo, Henricus A. M. Mutsaers, Rikke Norregaard
Summary: The study found that the selective estrogen receptor modulator tamoxifen has a mitigating effect on renal fibrosis in female, male, and ovariectomized female rats, as well as demonstrating an anti-fibrotic effect in human kidney slices. Gender differences may play a role in drug treatment response.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Ana Karina Aranda-Rivera, Alfredo Cruz-Gregorio, Omar Emiliano Aparicio-Trejo, Ariadna Jazmin Ortega-Lozano, Jose Pedraza-Chaverri
Summary: In the UUO model, overproduction of ROS leads to alterations in signaling pathways, promoting inflammation, oxidative stress, and apoptosis, contributing to fibrosis development. Impairment of mitochondrial metabolism is also involved in this process.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Review
Medicine, General & Internal
Yashu Wang, Xinna Deng, Zhaohua Yang, Haijiang Wu
Summary: This study analyzed the development trends of research on UUO-induced renal fibrosis between 2005 and 2022 using bibliometric and visual analysis. The results indicate that the molecular mechanism of UUO-induced renal fibrosis remains a hot topic, with a focus on the inflammatory response and oxidative stress. International cooperation is expected to expand and deepen in the future.
Article
Chemistry, Analytical
Leonoor E. M. Tideman, Lukasz G. Migas, Katerina Djambazova, Nathan Heath Patterson, Richard M. Caprioli, Jeffrey M. Spraggins, Raf Van de Plas
Summary: The study introduces an interpretable machine learning workflow that automatically identifies biomarker candidates by their mass-to-charge ratios and quantitatively estimates their relevance to recognizing a given biological class using SHAP. This methodology translates the task of biomarker discovery into a feature ranking problem, helping to rapidly screen thousands of molecular species for biomarker potential.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemistry & Molecular Biology
Bengt Uvelius, Karl-Erik Andersson
Summary: This study in rats aimed to identify the mechanisms that persist after the removal of obstruction in the lower urinary tract symptoms (LUTSs) due to prostatic enlargement. The results showed significant differences in mRNA expressions and ultrastructure between control, obstructed, and de-obstructed bladders, suggesting distinct properties in gene expressions, morphologies, and functional properties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Transplantation
Neslihan Tezcan, Zarife Nigar Ozdemir-Kumral, Naziye Ozkan Yenal, Ozlem T. Cilingir-Kaya, Aysin Tulunay Virlan, Dilek Ozbeyli, Sule Cetinel, Berrak C. Yegen, Mehmet Koc
Summary: The study demonstrates that NES-1 has renoprotective effects and can ameliorate renal fibrosis induced by unilateral ureteral obstruction. The mechanism may involve inhibition of neutrophil infiltration, reduction of oxidative stress, and inflammation. These findings suggest that NES-1 may play a regulatory role in protecting the kidneys against obstruction-induced renal injury.
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2022)
Article
Medicine, Research & Experimental
Ziqian Liu, Cuijuan Zhang, Juan Hao, Gege Chen, Lingjin Liu, Yunzhao Xiong, Yi Chang, Hui Li, Tatsuo Shimosawa, Fan Yang, Qingyou Xu
Summary: Chronic kidney disease (CKD) is associated with lung injury, and the severity of lung injury is related to lymphangiogenesis. Inhibition of mineralocorticoid receptors (MRs) can alleviate lung injury and lymphangiogenesis. In addition, transdifferentiation of lymphatic epithelial cells into myofibroblasts may be involved in lung fibrosis.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Cheng-Chieh Hung, Kuan-Hsing Chen, Hsiang-Hao Hsu, Ming-Yang Chang, Yi-Ching Ko, Huang-Yu Yang, Chih-Wei Yang
Summary: Noscapine has been found to alleviate renal fibrosis by reducing inflammation and inhibiting fibroblast transformation induced by TGF-beta 1, making it a potential therapeutic agent for inhibiting tubulointerstitial fibrosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Review
Biochemical Research Methods
Jianqiang Wu, Wei Wang, Zhaoran Chen, Fang Xu, Yuehong Zheng
Summary: Proteomics has been used to identify potential biomarkers, understand pathogenic mechanisms, and discover therapeutic targets for AAA. However, validation in multicenter cohorts is needed for promising biomarkers to accelerate their clinical application. The development of mass spectrometry technology, modification-specific proteomics, and multi-omics research will enhance our understanding of AAA pathogenesis and facilitate biomarker discovery and drug development for clinical translation.
EXPERT REVIEW OF PROTEOMICS
(2021)
Article
Pediatrics
Lulu Jia, Jianqiang Wu, Jing Wei, Lina Du, Panpan Wang, Yanju Zhang, Yuncui Yu, Xiaoling Wang, Yan Yang, Youhe Gao
Summary: Urinary protein biomarkers for the diagnosis and phenotyping of abdominal-type HSP were identified through comparing urinary proteomes among three syndromes, which can contribute to personalized treatment for patients with abdominal-type HSP.
TRANSLATIONAL PEDIATRICS
(2021)
Article
Multidisciplinary Sciences
Jinrui Ren, Jianqiang Wu, Xiaoyue Tang, Siliang Chen, Wei Wang, Yanze Lv, Lianglin Wu, Dan Yang, Yuehong Zheng
Summary: This study identified age-related differentially expressed proteins (DEPs) involved in abdominal aortic aneurysm (AAA) formation. The DEPs were associated with oxidative stress, coagulation and platelet activation, and complement and inflammation pathways. These findings suggest potential targets for AAA treatments.
Article
Immunology
Peng Liu, Jianqiang Wu, Dandan Sun, Haolong Li, Zhihong Qi, Xiaoyue Tang, Wei Su, Yongzhe Li, Xuzhen Qin
Summary: This study aimed to identify and validate candidate protein biomarkers for cryoglobulinemia (CGE) using a TMT-PRM-ELISA workflow. APOA1 and PCSK9 were confirmed to be increased in CGE patients, indicating the involvement of proteins in cholesterol metabolism in the development of CGE. These findings provide valuable insights for pathogenesis research and biomarker discovery in CGE.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Zhen Cao, Zejian Zhang, Xiaoyue Tang, Rui Liu, Mengwei Wu, Jianqiang Wu, Ziwen Liu
Summary: This study investigated the molecular mechanisms underlying lymph node metastasis (LNM) and its sex disparities in papillary thyroid microcarcinoma (PTMC) using a proteomics approach. The results showed that LNM in PTMC was associated with mitochondrial dysfunction and activation of oxidative phosphorylation and PI3K/AKT signaling pathways. The study also found distinct proteomics profiles between male and female PTMC patients with LNM, with elevated levels of extracellular matrix-associated proteins in male patients. Furthermore, certain proteins were identified to be downregulated and correlated with tumor metastasis, while others were upregulated and correlated with sex disparities and poor prognosis. These findings enhance our understanding of the functional networks and signaling pathways related to PTMC with LNM.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Zihao Wang, Yaning Wang, Mengqi Chang, Yuekun Wang, Peng Liu, Jianqiang Wu, Guige Wang, Xiaoyue Tang, Xiangyi Hui, Penghao Liu, Xiaopeng Guo, Bing Xing, Yu Wang, Zhijun Han, Wenbin Ma
Summary: This study identified the cellular origins and drivers of brain metastasis from lung adenocarcinoma (LUAD) at a single-cell level and developed a BM-index to indicate the risk of brain metastasis. They discovered brain metastasis-associated epithelial cells and their biomarker, and predicted therapeutic drugs targeting these cells. This study lays a foundation for early detection of high-risk LUAD patients.
Article
Medicine, General & Internal
Zhaoran Chen, Jianqiang Wu, Wei Wang, Xiaoyue Tang, Lei Zhou, Yanze Lv, Yuehong Zheng
Summary: This study investigated the functional mechanism and molecular targets of fluoroquinolones in relation to aortic aneurysm and dissection (AAD) through proteomic and network pharmacology analysis. Differential protein expression was observed in human aortic vascular smooth muscle cells (VSMCs) after ciprofloxacin (CIP) stimulation, highlighting the involvement of metabolism, extracellular matrix homeostasis, mitochondrial damage, focal adhesion, and apoptosis. Four critical target proteins, PARP1, RAC1, IGF1R, and MKI67, were identified through protein-protein interaction analysis. Signaling pathways such as MAPK, focal adhesion, apoptosis, actin cytoskeleton regulation, and PI3K-Akt were found to be significantly enriched. These findings provide novel insights into the pathogenic mechanism of fluoroquinolones in aortic diseases.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Jianqiang Wu, Wei Wang, Ting Xie, Zhaoran Chen, Lei Zhou, Xiaohong Song, Haoxuan Kan, Yanze Lv, Lianglin Wu, Fangda Li, Dan Yang, Yuexin Chen, Bao Liu, Yuehong Zheng
Summary: This study identified several promising plasma biomarkers of abdominal aortic aneurysm (AAA), including Legumain (LGMN), Delta-like protein 1 (DLL1), receptor tyrosine-protein kinase erbB-3 (ERBB3), and dipeptidyl peptidase 4 (DPPIV). These biomarkers have the potential to be therapeutic targets for AAA and further investigation is needed.
Article
Biochemistry & Molecular Biology
Ting Xie, Chuxiang Lei, Wei Song, Xunyao Wu, Jianqiang Wu, Fangyuan Li, Yanze Lv, Yuexin Chen, Bao Liu, Yuehong Zheng
Summary: Abdominal aortic aneurysm (AAA) is characterized by irreversible dilation of the infrarenal aorta. This study explored the variation of lipids during AAA development and found that lysophosphatidylcholines (lysoPCs) were specifically decreased in AAA patients and mice. The study also identified weakened positive correlations between lysoPCs and HDL-c in AAA patients, suggesting that HDL-lysoPCs may play a role in AAA pathogenesis. These findings suggest that lysoPCs could be promising biomarkers for AAA development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Xia Niu, Shuyu Zhang, Chen Shao, Zhengguang Guo, Jianqiang Wu, Jianling Tao, Ke Zheng, Wenling Ye, Guangyan Cai, Wei Sun, Mingxi Li
Summary: This study analyzed urinary complement proteins in IgA nephropathy (IgAN) patients using quantitative proteomics. The results showed that the abundance of complement proteins in the urine increased during IgAN progression, indicating the activation of the alternative pathway and coagulation pathway. The findings suggest that urinary complement proteins may serve as biomarkers for evaluating IgAN progression.
Article
Medicine, Research & Experimental
Yanze Lv, Guangchao Gu, Rong Zeng, Zhili Liu, Jianqiang Wu, Yuehong Zheng
Summary: Carotid body tumors (CBTs) are a rare type of paraganglioma that can only be effectively treated through surgical resection. This study aimed to investigate the molecular differences between different CBT types and identify potential therapeutic targets. Through proteomics analysis, the researchers found significant differences in protein profiles among different Shamblin subtypes of CBT. They also identified several signaling pathways that are significantly enriched in CBT. Furthermore, the researchers identified key differentially expressed proteins (DEPs) and validated them using immunohistochemistry. This study provides valuable insights into the pathogenesis of CBT and may contribute to the development of targeted therapies.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2023)
Article
Multidisciplinary Sciences
Chuxiang Lei, Haoxuan Kan, Xiangyu Xian, Wenlin Chen, Wenxuan Xiang, Xiaohong Song, Jianqiang Wu, Dan Yang, Yuehong Zheng
Summary: This study discovers that the metabolic cytokine FAM3A is decreased in AAA patients and murine models, and overexpression or supplementation of FAM3A can significantly reduce AAA formation. FAM3A affects VSMC differentiation and inhibits their transformation into other cell types by regulating KLF4 ubiquitination and nuclear localization.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Mindi Zhao, Yameng Zhang, Jianqiang Wu, Xundou Li, Youhe Gao
Summary: Multiple sclerosis is a chronic autoimmune demyelinating disease of the central nervous system that is difficult to diagnose in early stages. A study analyzed the early urinary proteome changes in a multiple sclerosis rat model and identified potential biomarkers for early detection. Functional analysis showed that these proteins are associated with asparagine degradation, neuroinflammation, and lipid metabolism. Early treatment with methylprednisolone significantly reduced the incidence of encephalomyelitis. Urine may be a good source of biomarkers for early diagnosis of multiple sclerosis.
ROYAL SOCIETY OPEN SCIENCE
(2023)
Letter
Medicine, Research & Experimental
Xiaoyue Tang, Yanan Zhou, Jianqiang Wu, Peng Liu, Yehong Yang, Qiaochu Wang, Shuaiyao Lu, Jiangfeng Liu, Juntao Yang
Article
Oncology
Zejian Zhang, Zhen Cao, Rui Liu, Zepeng Li, Jianqiang Wu, Xiaoli Liu, Mengwei Wu, Xiequn Xu, Ziwen Liu
Summary: This study characterized the N-glycome of papillary thyroid microcarcinoma (PTMC), and established nomograms for the diagnosis of PTMC and the prediction of lymph node metastasis (LNM). The study found significant differences in serum N-glycome between PTMC and healthy controls, as well as associations between certain N-glycan traits and PTMC and LNM. Nomograms based on these traits showed good performance in the identification of PTMC and the prediction of LNM.