Journal
SCIENTIFIC REPORTS
Volume 4, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/srep06866
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Funding
- Australian Research Council [DP110103015]
- NIH LAMP Biotechnology Resource [P41EB015890]
- US Air Force Office of Scientific Research [AFOSR FA9550-08-1-0384]
- Beckman Laser Institute Foundation
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Quantitative determination of the motility forces of chromosomes during cell division is fundamental to understanding a process that is universal among eukaryotic organisms. Using an optical tweezers system, isolated mammalian chromosomes were held in a 1064 nmlaser trap. The minimum force required to move a single chromosome was determined to be approximate to 0.8-5 pN. The maximum transverse trapping efficiency of the isolated chromosomes was calculated as approximate to 0.01-0.02. These results confirm theoretical force calculations of approximate to 0.1-12 pN to move a chromosome on the mitotic or meiotic spindle. The verification of these results was carried out by calibration of the optical tweezers when trapping microspheres with a diameter of 4.5-15 mu m in media with 1-7 cP viscosity. The results of the chromosome and microsphere trapping experiments agree with optical models developed to simulate trapping of cylindrical and spherical specimens.
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