Article
Endocrinology & Metabolism
Kai Otsuka, Shin Matsubara, Akira Shiraishi, Natsumi Takei, Yui Satoh, Miho Terao, Shuji Takada, Tomoya Kotani, Honoo Satake, Atsushi P. Kimura
Summary: The newly discovered testis-specific lncRNA, Start, is involved in testicular steroid activation, and its knockout leads to reduced testosterone levels, affecting spermatogenesis, but does not show significant abnormalities in adult mice.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Genetics & Heredity
Meghana Manjunath, Snehal Nirgude, Anisha Mhatre, Sai G. Vemuri, Mallika Nataraj, Jayanti Thumsi, Bibha Choudhary
Summary: Breast cancer is one of the leading causes of cancer-associated death in women. Despite progress in treatment, drug resistance and recurrence still impact patient survival. This study aimed to identify lncRNA and mRNA signatures in Indian breast cancer patients using RNA-seq. The analysis revealed a gene set that could segregate the different molecular subtypes of breast cancer. Survival analysis showed that menopausal status, tumor grade, and hormone status significantly affected the risk of mortality due to breast cancer.
FRONTIERS IN GENETICS
(2022)
Article
Multidisciplinary Sciences
Kai Otsuka, Hong Yang, Shin Matsubara, Akira Shiraishi, Misuzu Kurihara, Honoo Satake, Atsushi P. Kimura
Summary: This study revealed a novel role of a long noncoding RNA named Start in germ cells. Start was found to regulate the transcriptional activation of the Prss43/Tessp-3 gene in spermatocytes, providing new insights into the multifunctionality of IncRNAs in the testis.
Article
Multidisciplinary Sciences
Qianxing Zou, Lele Yang, Ruona Shi, Yuling Qi, Xiaofei Zhang, Huayu Qi
Summary: The study found that the ribosomal large subunit protein gene RPL39L is essential for mouse spermatogenesis, as its deletion leads to reduced fertility. Furthermore, the lack of RPL39L also affects the sperm mitochondria and flagella, which in turn affects sperm quality. These results demonstrate that maintaining proteostasis is crucial for spermatogenesis.
Article
Genetics & Heredity
Indu Sharma, Jun Fang, Eric A. Lewallen, Kirk W. Deitsch, Thomas F. McCutchan
Summary: Protozoan parasites of the genus Plasmodium cause malaria and undergo profound changes in morphology, host cell tropism, and gene expression during transition from human host to insect vector. They have a unique ability to adapt to major environmental changes through differential expression of stage-specific ribosomal RNAs.
Article
Genetics & Heredity
Yuanjun Li, Ying Lu, Kaixuan Du, Yewei Yin, Tu Hu, Qiuman Fu, Yanni Zhang, Dan Wen, Xiaoying Wu, Xiaobo Xia
Summary: This study identified the differential expression profiles of lncRNAs in myopic mouse retinas and explored their possible roles in various biological processes. These findings provide important scientific evidence for the study of myopia and the development of potential interventions.
FRONTIERS IN GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Yanzhen Nie, Geng. G. Tian, Longbin Zhang, Trevor Lee, Zhen Zhang, Jing Li, Tao Sun
Summary: Proper development of the mammalian cerebral cortex relies on precise gene expression regulation, which is influenced by both mRNAs and long noncoding RNAs (lncRNAs) along with N(6)-methyladenosine (m(6)A) modifications. Analysis of RNA sequencing and methylated RNA immunoprecipitation sequencing reveals differential expression of lncRNAs and stage-specific m(6)A-methylation patterns in the developing cortex of mouse embryos and postnatal subjects. The findings highlight the importance of m(6)A-mediated epitranscriptomic modifications in temporally expressed lncRNAs and their correlation with transcript abundance.
Article
Biochemistry & Molecular Biology
Shuwei Ren, Ning Zhang, Liping Shen, Yongyong Lu, Yixin Chang, Zhenni Lin, Ning Sun, Yuanmei Zhang, Jiheng Xu, Haishan Huang, Honglei Jin
Summary: The study found that the long noncoding RNA lnc00892 is significantly downregulated in bladder cancer tissues and its low expression is associated with poor prognosis. Lnc00892 was shown to inhibit migration, invasion, and metastasis of bladder cancer cells by reducing nucleolin gene transcription and inhibiting nucleolin-mediated stabilization of RhoA/RhoC mRNA. These findings suggest that lnc00892 may serve as a potential therapeutic target for invasive bladder cancer patients.
Article
Plant Sciences
Ning Li, Tongtong Liu, Feng Guo, Jinwen Yang, Yugang Shi, Shuguang Wang, Daizhen Sun
Summary: This study identified lncRNAs, miRNAs, and genes related to drought stress in wheat through whole-transcriptome sequencing and explored their regulatory modules. The results revealed the potential role of these regulatory modules in wheat drought tolerance.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Chaoqiong Ding, Xiang Yan, Mengying Xu, Ran Zhou, Yuancun Zhao, Dan Zhang, Zongyao Huang, Zhenzhong Pan, Peng Xiao, Huifang Li, Lu Chen, Yuan Wang
Summary: This study reports the full-length transcriptome of mouse neural stem cells (NSCs) at different developmental stages, providing an important resource for studying NSC fate determination, neural development, and disease in the neuroscience community.
Article
Biotechnology & Applied Microbiology
Dongliang Yu, Yuqing Wu, Leilei Zhu, Yuying Wang, Donglai Sheng, Xiaofeng Zhao, Guoqing Liang, Lin Gan
Summary: Based on established gene models and ab initio prediction, this study characterized 4,523 lncRNA genes in developing mouse retinas, providing the most comprehensive collection of retinal lncRNAs to date. These lncRNAs were found to be highly spatiotemporal specific in expression and played essential roles in regulating retinal genesis and function. The expression of certain lncRNAs was found to be implicated in the regulation of retinal neuron differentiation and development through specific pathways.
Article
Multidisciplinary Sciences
Pedro Patraquim, Emile G. Magny, Jose Pueyo, Ana Isabel Platero, Juan Pablo Couso
Summary: Ribosomal Profiling in Drosophila embryos detected the translation of 100 to 300 micropeptides from small ORFs within lncRNA, indicating the conservation of micropeptide sequence and function. Around 30% of lncRNAs contain small ORFs engaged by ribosomes, leading to regulated translation of micropeptides. Furthermore, there is evidence of natural selection favoring the conservation of micropeptide sequence and function across evolution.
NATURE COMMUNICATIONS
(2022)
Review
Medicine, Research & Experimental
Enjun Lv, Jiaqi Sheng, Chengpeng Yu, Dean Rao, Wenjie Huang
Summary: Long noncoding RNAs (lncRNAs) have gained widespread attention as new and crucial molecules involved in biological function regulation, particularly in tumor metastasis such as hepatocellular carcinoma; despite the incomplete understanding of their mechanisms, lncRNAs have been shown to be closely associated with various stages of tumor growth, invasion, and metastasis.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Plant Sciences
Kuan-Chieh Tseng, Nai-Yun Wu, Chi-Nga Chow, Han-Qin Zheng, Chin-Yuan Chou, Chien-Wen Yang, Ming-Jun Wang, Song-Bin Chang, Wen-Chi Chang
Summary: This study developed JustRNA, an expression profiling resource for plant lncRNAs. The platform contains a large number of lncRNA annotations and RNA-seq samples for multiple plant species. Functional network reconstruction, genomic association analysis, microRNA target prediction, and co-expression analysis were used to investigate the regulatory mechanisms of plant lncRNAs. The integration of chromatin immunoprecipitation sequencing data further identified the transcriptional regulation of lncRNAs in several plant species.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Multidisciplinary Sciences
Neda Mokhberian, Kazem Sharifi, Ehsan Soleimaninejadian, Mohamad Eftekhary, Seyed Mahmoud Hashemi, Shohreh Farhadi, Satomi Miwa, Hossein Ghanbarian
Summary: This study demonstrates that upregulation of SIRT1 can be achieved by knocking down the natural antisense transcript SIRT1-NAT, leading to improved therapeutic potentials of human MSCs. Knockdown of SIRT1-NAT enhances the proliferation and differentiation abilities of MSCs, reduces senescence-associated activity, and reverses senescence-associated molecular changes.
SCIENTIFIC REPORTS
(2022)
Article
Biology
Mengxi Guo, Chengliang Zhou, Gufeng Xu, Lin Tang, Yechun Ruan, Ying Yu, Xianhua Lin, Dandan Wu, Hao Chen, Priscilla Yu, Luyang Jin, Yinyu Wang, Yimei Wu, Kamran Ullah, Tanzil Ur Rahman, Xinmei Liu, Jianzhong Sheng, Hsiao-Chang Chan, Hefeng Huang
SCIENCE CHINA-LIFE SCIENCES
(2020)
Review
Biology
Lu Wen, Qiang Liu, Jingjing Xu, Xixi Liu, Chaoyi Shi, Zuwei Yang, Yili Zhang, Hong Xu, Jiang Liu, Hui Yang, Hefeng Huang, Jie Qiao, Fuchou Tang, Zi-Jiang Chen
SCIENCE CHINA-LIFE SCIENCES
(2020)
Article
Obstetrics & Gynecology
Rui-Hong Xue, Juan Li, Zhen Huang, Zheng-Zheng Li, Lei Chen, Qin Lin, He-Feng Huang
ARCHIVES OF GYNECOLOGY AND OBSTETRICS
(2020)
Article
Clinical Neurology
Han Liu, Hong Li, Cheng Li, Lei Chen, Chenjie Zhang, Zhiwei Liu, Yanting Wu, Hefeng Huang
Summary: The study revealed a negative association between maternal sleep quality during pregnancy and birth weight z-scores in female newborns, but not in males. Additionally, poor sleep quality in late pregnancy was identified as a risk factor for low birth weight.
BEHAVIORAL SLEEP MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Ling Gao, Dandan Wu, Yanting Wu, Zuwei Yang, Jianzhong Sheng, Xianhua Lin, Hefeng Huang
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Chuan-Jin Yu, Xia Liu, Zhi-Yang Zhou, Xiao-Jun Chen, Yi-Cong Meng, Hang-Chao Gu, Jing-Jing Xu, Guo-Lian Ding, Xin-Mei Liu, Jian-Zhong Sheng, He-Feng Huang
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Cell Biology
Catrina Spruce, Sibongakonke Dlamini, Guruprasad Ananda, Naomi Bronkema, Hui Tian, Kenneth Paigen, Gregory W. Carter, Christopher L. Baker
GENES & DEVELOPMENT
(2020)
Article
Peripheral Vascular Disease
Dan-dan Wu, Ling Gao, Ou Huang, Kamran Ullah, Meng-xi Guo, Ye Liu, Jian Zhang, Lei Chen, Jian-xia Fan, Jian-zhong Sheng, Xian-hua Lin, He-feng Huang
Article
Hematology
Cheng Li, Yun-Fei Su, Li Jin, Yan-Yan Guo, Guo-Lian Ding, Lin Tang, Mei Cai, He-Feng Huang, Xin-Mei Liu
THROMBOSIS RESEARCH
(2020)
Letter
Obstetrics & Gynecology
Xue Rui-hong, Lin Xian-hua, Wu Dan-dan, Huang He-Feng
ACTA OBSTETRICIA ET GYNECOLOGICA SCANDINAVICA
(2020)
Review
Biochemistry & Molecular Biology
Xinxin Ke, Tao Hu, Mizu Jiang
Summary: The cGAS-STING signaling pathway plays a crucial role in GI inflammatory diseases and cancers. Understanding the function and mechanism of this pathway is helpful for developing therapeutic strategies.
Article
Chemistry, Analytical
Tao Hu, Xinxin Ke, Yangjing Ou, Yu Lin
Summary: In this study, a CRISPR/Cas12a-triggered chemiluminescence enhancement biosensor named CRICED was developed for ultrasensitive nucleic acid detection. The biosensor showed high sensitivity and specificity in detecting synthetic DNA targets, amplified DNA plasmids, and clinical samples, demonstrating its potential for practical applications in CRISPR-based diagnostics.
ANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Xinxin Ke, Yangjing Ou, Yu Lin, Tao Hu
Summary: In this study, a chemiluminescence enhancement biosensor based on CRISPR/Cas12a with HCR amplifying strategy was developed for nucleic acids detection. The biosensor showed high sensitivity and specificity, low cost, and visual imaging. The detection limit for synthetic DNA target was 3 pM. The biosensor also exhibited excellent sensitivity and specificity when applied to HPV clinical samples.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Multidisciplinary
Tao Hu, Xinxin Ke, Wei Li, Yu Lin, Ajuan Liang, Yangjing Ou, Chuanxia Chen
Summary: CRISPR-RDB is an affordable and sensitive multiplex target detection method that combines CRISPR-Cas12a with a reverse dot blot technique. It can simultaneously detect multiple targets and convert the results into visual color signals. This method can not only rapidly detect pathogens such as influenza viruses, respiratory syncytial virus, and SARS-CoV-2, but also be modified for detection of other targets with simple modifications.
Article
Chemistry, Analytical
Xinxin Ke, Yi Hu, Chuanxia Chen, Tao Hu
Summary: In this study, a one-tube dual-readout biosensor (CRISAT) was developed using CRISPR-ALP tandem assay for the detection of ultrasensitive nucleic acids and non-nucleic acids. By activating Cas12a to cut the hyDNA sequence, abundant ALP is released, leading to the generation of Si PCDs or PCDs. This method offers a fluorescence and colorimetry dual-readout nanoplatform for CRISPR-based biosensors with high sensitivity and specificity.
