Article
Biochemical Research Methods
Chris Weise, Hannes Westphal, Rico Warias, Detlev Belder
Summary: Here, a miniaturized chip-based HPLC approach coupled with electrospray ionization mass spectrometry is introduced, which utilizes temperature to achieve high-speed separations. With the advantages of low thermal mass of the microfluidic chip and pre-heated mobile phase to form an electrospray, isothermal and temperature-programmable operations were conducted to separate pesticides in methanol and ethanol-containing eluents in less than 20 s.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Ting Wang, Ting Zhou, Kang Wu, Junlin Cao, Yuze Feng, Jianguo Li, Anping Deng
Summary: Zearalenone (ZEN) is a highly toxic mycotoxin commonly found in agricultural products. This study developed a sensitive ELISA method for detecting ZEN in food and feed samples, utilizing a ZEN derivative to synthesize monoclonal antibodies (mAb) and selecting hybridoma clones capable of secreting antibodies against ZEN. The proposed ELISA demonstrated high sensitivity for ZEN detection and no cross-reactivity with other mycotoxins.
Article
Chemistry, Applied
Yunling Shao, Miao Wang, Jing Cao, Yongxin She, Zhen Cao, Zhenxia Hao, Fen Jin, Jing Wang, A. M. Abd El-Aty
Summary: In this study, a method was developed to rapidly detect organophosphorus pesticides (OPs) by combining TLC and enzyme inhibition principles. After removing organic solvents from the samples using TLC and paper-based chips, the enzyme was introduced to the detection system. The results demonstrated that this method effectively minimized the influence of solvents on enzyme behavior. Additionally, the pigments could be retained on TLC using a 40% ddH2O/ACN solution as a developing solvent. The method exhibited high sensitivity and provided valuable insights for sample pretreatment and rapid determination of pesticide residues in food.
Review
Chemistry, Analytical
Stian Kogler, Kristina Saeterdal Komurcu, Christine Olsen, Jun-ya Shoji, Froydis Sved Skottvoll, Stefan Krauss, Steven Ray Wilson, Hanne Roberg-Larsen
Summary: Organoids and organ-on-a-chip devices are important tools for studying various aspects of organs. This review focuses on the application of mass spectrometry in studying organoids and organ-on-a-chip-derived material. It discusses the use of liquid chromatography-mass spectrometry (LC-MS) for proteomics, metabolomics/lipidomics, and hormones, as well as mass spectrometry imaging (MSI) of organoids. The analysis has provided insights into the development and disease of organs, potentially replacing or complementing animal models.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Hanne Roberg-Larsen, Steven Ray Wilson, Elsa Lundanes
Summary: This review focuses on the application of nLC-MS in sample preparation, particularly through online upfront devices and online reaction systems to improve the sensitivity and robustness of samples.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Bo Wang, Sheng Zhang, Zi-Tao Shen, Ting Hou, Yi-Han Zhao, Meng-Sheng Huang, Jian Li, Huan Chen, Peng-Hui Hu, Zi-Jiang Luo, Shuai Yuan, Feng-Min Wang, Wei Li, Chang Shu, Xing-Hua Xia, Ya Ding
Summary: Enzyme-prodrug therapies have advantages in in vivo prodrug activation, but precise control and depletion of substrate remain challenges. This study introduces a core-shell reactor using ZIF-8 to partition enzyme and prodrug, improving prodrug conversion efficiency and tumor suppression rate through targeted delivery and increased drug loading capacity.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Jackie Jackie, Chun Kiang Chua, Douglas Chiang Yon Chong, Si Ying Lim, Sam Fong Yau Li
Summary: The study demonstrates the capability of Py-GC-MS for direct analysis of endotoxins, with the SS Py-GC-MS method showing superior potential in identifying bacterial endotoxins effectively.
Article
Engineering, Environmental
Yeon Hee Ro, Kwang Young Kim, Soon Kwan Jeong, Geun Bae Rhim, Dae Hyun Moon, Jun Eo, Hyeon Song Lee, Gyoung Woo Lee, Bo Young Lim, Dong Hyun Chun, Jinwon Park, Min Hye Youn
Summary: To achieve carbon neutrality through CO2 mineralization, a novel reaction process was designed based on a thorough understanding of the critical reaction steps. The introduction of CA enzyme was found to promote the reaction rate, but enzyme loss occurred during the reaction. A membrane-separated reactor was developed to minimize enzyme loss and enhance the CO2 mineralization efficiency. The CA enzyme showed higher effectiveness in the K2CO3 aqueous solution compared to DI water.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Analytical
Fei-Long Liu, Tian-Tian Ye, Jiang-Hui Ding, Xiao-Ming Yin, Xiao-Ke Yang, Wei-Hua Huang, Bi-Feng Yuan, Yu-Qi Feng
Summary: Researchers synthesized paired reagents of DMPI and d(3)-DMPI, which can efficiently react with phosphate groups on polar phosphorylated metabolites. They found that tagging DMPI onto ribonucleotides significantly increased ionization efficiencies during LC/MS analysis, resulting in enhanced detection sensitivities.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Yan Zhang, Sungho Kim, Weihua Shi, Yaoyao Zhao, Insu Park, Christopher Brenden, Hrishikesh Iyer, Prasoon Jha, Rashid Bashir, Jonathan V. Sweedler, Yurii Vlasov
Summary: The silicon microfluidic platform allows for the monolithic integration of transparent micron-scale microfluidic channels, on-chip segmentation of analyte flows into picoliter droplets, and spatial and temporal separation of oil and aqueous phases during electro-spray for subsequent mass spectrometry analysis.
Article
Chemistry, Analytical
Yi Dai, Chu-Bo Qi, Yang Feng, Qing-Yun Cheng, Fei-Long Liu, Ming-Yu Cheng, Bi-Feng Yuan, Yu-Qi Feng
Summary: The discovery of dynamic and reversible modifications in RNA has expanded their functional repertoires, offering new insight into biological processes. A novel chemical derivatization strategy combined with mass spectrometry analysis has been developed to sensitively and simultaneously determine uridine thiolation and hydroxylation modifications in eukaryotic RNA, enhancing detection sensitivity by up to 339-fold. This method has been used to identify a new modification of 5-hydroxyuridine (ho(5)U) in mammalian cell small RNAs, demonstrating superior capability in detecting low-abundance RNA modifications and potentially leading to the discovery of new modifications in RNA.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Aleksandra Antevska, Connor C. Long, Samuel D. Dupuy, J. Jason Collier, Michael D. Karlstad, Thanh D. Do
Summary: We used liquid chromatography coupled with ion mobility spectrometry-mass spectrometry to investigate the effects of corticosterone on mouse pancreatic islet hormones. Our results show that insulin 1 (Ins1) and insulin 2 (Ins2) have distinct structures and degradation rates. Ins2 may exist in both T and R states, while Ins1 may not. The differences in structure and degradation account for their unique characteristics and the diabetes risk associated with Ins1. Additionally, we observed an increase in the ratio of amylin to Ins1 in corticosterone-treated mice, which correlated with beta-cell proliferation.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Chemistry, Analytical
Zhenbin Zhang, Norman J. Dovichi
Summary: This study presents an integrated platform that combines a miniaturized filter-aided sample preparation method with high-pH reversed phase or strong cation exchange microreactors for efficient sample preparation and fractionation prior to LC-ESI-MS/MS analysis. The platform generated a large number of unique peptides and protein groups, comparable to state-of-the-art methods, but with reduced cost and time. This integrated platform has significant potential in deep proteomics analysis of mass-limited samples.
ANALYTICAL CHEMISTRY
(2022)
Article
Environmental Sciences
J. M. S. Oliveira, C. A. Sabatini, A. J. Santos-Neto, E. Foresti
Summary: Most studies on the degradation of textile azo dyes focus on color as a parameter, but it is susceptible to interference. This study proposes a method for determining a sulfonated azo dye in wastewater using solid-phase extraction and liquid chromatography - electrospray ionization tandem mass spectrometry. The results showed that the dye undergoes hydrolysis and has structural changes in aqueous solution, but its dyeing capacity remains unchanged. Color remains a reliable parameter for monitoring unstable azo compounds in aqueous solution, but a more robust method is needed for the speciation of the dye's hydrolyzed products.
ENVIRONMENTAL POLLUTION
(2023)
Article
Nanoscience & Nanotechnology
Yunji Eom, Byeonghwa Lim, Keonmok Kim, Taehyeong Jeon, Changyeop Jeon, Sunjong Oh, Hyeonseol Kim, Proloy T. Das, Sri Ramulu Torati, CheolGi Kim
Summary: This study developed an on-chip magnetometer for optimizing the synthesis conditions of magnetic nanoparticles (MNPs). The magnetometer enables parallel synthesis and measurement, and the induced field from the synthesized particles is directly detected using a microreactor. The experiments successfully optimized the synthesis conditions in a short time and demonstrated the flexibility of the developed on-chip magnetometer.
JOURNAL OF SCIENCE-ADVANCED MATERIALS AND DEVICES
(2022)
Article
Toxicology
Marianne Skov-Skov Bergh, Inger Lise Bogen, Elisabeth Nerem, Ariane Wohlfarth, Steven Ray Wilson, Ase Marit Leere Oiestad
Summary: This study aimed to discover and elucidate the major metabolites of three novel fentanyl analogues, providing important markers for confirming the intake of these compounds in forensic cases.
FORENSIC TOXICOLOGY
(2021)
Review
Chemistry, Analytical
Hanne Roberg-Larsen, Steven Ray Wilson, Elsa Lundanes
Summary: This review focuses on the application of nLC-MS in sample preparation, particularly through online upfront devices and online reaction systems to improve the sensitivity and robustness of samples.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Eleonora Pizzi, Trine Gronhaug Halvorsen, Christian J. Koehler, Leon Reubsaet
Summary: This study describes the immobilization of trypsin on cellulose paper using different methods, enabling a fundamental understanding of protein binding and enzyme activity on paper. Functionalized discs outperform untreated discs in protein binding, with potential demonstrated in digesting complex biological samples.
Article
Immunology
Erik Waage Nielsen, Yoav Miller, Ole-Lars Brekke, Joost Grond, Anh Hoang Duong, Hilde Fure, Judith Krey Ludviksen, Kristin Pettersen, Leon Reubsaet, Rigmor Solberg, Harald Thidemann Johansen, Tom Eirik Mollnes
Summary: This study established a new porcine model to evaluate systemic ischemia-reperfusion injury. The results showed that treatment with C1-INH had little effect on the changes induced by IRI.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Analytical
Christine Olsen, Elisa Wiborg, Elsa Lundanes, Shadab Abadpour, Hanne Scholz, Steven Ray Wilson
Summary: The research focuses on investigating tools for coupling pancreas-on-a-chip systems with mass spectrometry. By using an inter-chain cleavage reactor and nano liquid chromatography-mass spectrometry system, the researchers achieved on-line cleavage of insulin's disulfide bonds and obtained robust fragmentation mass spectra. The use of a silica monolith in the nano liquid chromatography format improved chromatographic performance compared to particle-packed columns.
SEPARATION SCIENCE PLUS
(2022)
Article
Chemistry, Analytical
Minh Thao Nguyen, Trine Gronhaug Halvorsen, Bernd Thiede, Leon Reubsaet
Summary: This study investigates the digestion of proteins by trypsin covalently bound to paper compared to trypsin in solution. The results show that trypsin on paper retains approximately 50% of its activity and exhibits greater stability than trypsin in solution. In-solution digestions also yield more protein identifications and higher coverage compared to on-paper digestions.
SEPARATION SCIENCE PLUS
(2022)
Article
Biochemical Research Methods
Christina Johannsen, Anam Ul Haq, Leon Reubsaet, Trine Gronhaug Halvorsen
Summary: The study introduces a novel on-paper device combining sample collection with affinity sample pretreatment. The functionality of the device has been demonstrated in the laboratory setting, showing potential for detecting low abundant biomarkers.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Review
Biochemical Research Methods
Trine Gronhaug Halvorsen, Leon Reubsaet
Summary: Selective and efficient sample clean-up is important in mass spectrometric protein and proteomics analyses. This review discusses the utility of molecularly imprinted polymers (MIPs) for protein capture from biological matrices prior to mass spectrometry (MS) analysis. The review covers the application of MIPs in depletion of abundant proteins, protein and proteotypic peptide capture, and capture of post-translational modifications (PTMs), as well as provides an overview of available MIP formats and mass spectrometric techniques used in protein analysis after MIP capture. Overall, the review highlights that MIPs for sample clean-up in mass spectrometric protein and proteomics analysis from biological matrices is still not fully matured.
Article
Chemistry, Analytical
Stian Kogler, Aleksandra Aizenshtadt, Sean Harrison, Froydis Sved Skottvoll, Henriette Engen Berg, Shadab Abadpour, Hanne Scholz, Gareth Sullivan, Bernd Thiede, Elsa Lundanes, Inger Lise Bogen, Stefan Krauss, Hanne Roberg-Larsen, Steven Ray Wilson
Summary: Organoids, laboratory-grown organ models developed from stem cells, have been successfully coupled with liquid chromatography-mass spectrometry (LC-MS) to enable continuous tracking of drug metabolism.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Huan Bao Ngo, Inger Oulie, Leon Reubsaet, Trine Gronhaug Halvorsen
Summary: This paper presents a protocol for efficient sample cleanup of low-abundance proteins using bead-based proteolysis and LC-MS/MS determination. It also includes detailed procedures for bead preparation and determination of biomarkers in dried serum. Results may vary depending on the sampling material, with higher signal intensities observed for samples collected using VAMS compared to DSSs.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Review
Chemistry, Analytical
Hanne Bendiksen Skogvold, Helge Rootwelt, Leon Reubsaet, Katja Benedikte Presto Elgstoen, Steven Ray Wilson
Summary: Dried blood spot samples offer a simple and safe option for diagnostics, with liquid chromatography-mass spectrometry being a versatile measurement tool. These samples provide valuable information for metabolomics, xenobiotic analysis, and proteomics. Targeted analysis of small molecules is the main application, but there are emerging applications in untargeted metabolomics and proteomics. Applications range from newborn screening to disease diagnostics and monitoring, as well as research into the effects of diet, exercise, xenobiotics, and doping. A variety of dried blood spot products and liquid chromatography-mass spectrometry methods are available, including novel approaches like on-paper sample preparation.
JOURNAL OF SEPARATION SCIENCE
(2023)
Review
Chemistry, Analytical
Stian Kogler, Kristina Saeterdal Komurcu, Christine Olsen, Jun-ya Shoji, Froydis Sved Skottvoll, Stefan Krauss, Steven Ray Wilson, Hanne Roberg-Larsen
Summary: Organoids and organ-on-a-chip devices are important tools for studying various aspects of organs. This review focuses on the application of mass spectrometry in studying organoids and organ-on-a-chip-derived material. It discusses the use of liquid chromatography-mass spectrometry (LC-MS) for proteomics, metabolomics/lipidomics, and hormones, as well as mass spectrometry imaging (MSI) of organoids. The analysis has provided insights into the development and disease of organs, potentially replacing or complementing animal models.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Michal Gregus, Alexander R. Ivanov, Steven Ray Wilson
Summary: Ultralow flow LC utilizes ultra-narrow bore columns and flow rates ranging from pL/min to low nL/min. It provides high sensitivity and resolution, making it suitable for analyzing scarce biological samples. Recent advancements in dedicated instrumentation, technical approaches, and higher throughput have facilitated the application of ultralow flow LC to the analysis of amount-limited samples. This review discusses the types of ultralow flow LC technology, highlights representative applications in bioanalysis, and explores future developments.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Chemistry, Analytical
Trine Gronhaug Halvorsen, Leon Reubsaet
Summary: In 2017, a smart sampling technique was introduced, allowing for sample preparation of proteins to begin at the moment of sampling, reducing the need for extensive sample preparation in the lab. This approach reshuffles the conventional protein analysis workflow, simplifying the sample preparation steps required upon sample arrival in the lab.
ANALYTICAL SCIENCE ADVANCES
(2023)