Article
Chemistry, Multidisciplinary
Euihyun Lee, Carlos R. Baiz
Summary: This study characterizes the interactions between DMSO and water, showing that DMSO disrupts the hydrogen bond networks and this disruption increases with decreasing temperature.
Review
Fisheries
Amirreza Abed-Elmdoust, Ongun Uysal, Ruhollah Rahimi, Yasamine Farahmand
Summary: The study evaluated the effectiveness of antifreeze proteins in fish sperm cryopreservation and found that antifreeze proteins type I and III significantly preserved sperm motility and viability. Future studies should focus on the combined effect of heterogeneous sources.
Article
Biochemistry & Molecular Biology
Daniel Norman, Carl Johan Drott, Per-Ola Carlsson, Daniel Espes
Summary: Irisin is a myokine involved in glucose homeostasis that is found in human and rat islets. Its secretion is regulated by glucose, but it does not affect insulin and glucagon secretion. However, Irisin does reduce islet blood flow without affecting insulin secretion.
Article
Cell & Tissue Engineering
Kenyu Nakayama-Iwatsuki, Masumi Hirabayashi, Shinichi Hochi
Summary: The study demonstrates that pancreatic islet single cells prepared using different freezing methods can successfully generate pseudo-islets with normal functionality and appropriate size. These pseudo-islets exhibit sufficient response to high glucose stimulation and have a similar arrangement of endocrine cells as native islets.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Mengying Wang, Plamen Todorov, Wanxue Wang, Evgenia Isachenko, Gohar Rahimi, Peter Mallmann, Vladimir Isachenko
Summary: This study found that freezing spermatozoa leads to more down-regulation of genes, which is related to apoptosis changes and immune response. Vitrification induces minor biological changes in human spermatozoa compared to conventional freezing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Obstetrics & Gynecology
Yodo Sugishita, Lingbo Meng, Yuki Suzuki-Takahashi, Sandy Nishimura, Sayako Furuyama, Atsushi Uekawa, Akiko Tozawa-Ono, Junko Migitaka-Igarashi, Tomoe Koizumi, Hibiki Seino, Yasunori Natsuki, Manabu Kubota, Junki Koike, Keisuke Edashige, Nao Suzuki
Summary: After thawing/warming, at least 60 minutes of diffusion washing in media was necessary to significantly reduce the residual cryoprotectants in ovarian tissues frozen by slow freezing or vitrification.
HUMAN REPRODUCTION
(2022)
Review
Endocrinology & Metabolism
Neil Tanday, Andrei I. Tarasov, R. Charlotte Moffett, Peter R. Flatt, Nigel Irwin
Summary: The development of pancreatic islet endocrine cells is tightly regulated, and small changes in the environment can lead to the generation of different cell types with distinct hormones. Cell differentiation is driven by transcription factors, which are also critical for maintaining mature islet cell phenotype. Prolonged metabolic stress can alter the transcription factor set of insulin-secreting beta cells, leading to loss of beta-cell identity through de- or transdifferentiation. Approved and experimental antidiabetic agents have been associated with preventing beta-cell dedifferentiation or promoting the transdifferentiation of non-beta cells towards an insulin-positive beta-cell-like phenotype. Understanding islet cell plasticity is important for preventing beta-cell decline in diabetes.
DIABETES OBESITY & METABOLISM
(2023)
Review
Biochemistry & Molecular Biology
Serena Barachini, Letizia Biso, Shivakumar Kolachalam, Lacopo Petrini, Roberto Maggio, Marco Scarselli, Biancamaria Longoni
Summary: Pancreatic islet transplantation is a treatment for Type 1 diabetic patients, and mesenchymal stem cells (MSCs) have shown potential in controlling graft rejection. MSCs can interact with immune system cells through direct contact or their secretome. This review discusses the role of MSCs in controlling the immune system and promoting repair in pancreatic islet transplantation, as well as challenges such as oxidative stress and impaired vasculogenesis. Preconditioning MSCs with hypoxia exposure or using stem cells with angiogenic potential in organoids may help improve transplant outcomes.
Article
Biology
Kenyu Nakayama-Iwatsuki, Kotaro Yanagisawa, Dan Tanaka, Masumi Hirabayashi, Jun Negishi, Shinichi Hochi
Summary: The study shows that pre- or post-treatment of rat islets with liver decellularized matrix (LDM) can improve the viability and functionality of damaged islets in the vitrification process.
Article
Endocrinology & Metabolism
Yu-Chin Lien, Xueqing Maggie Lu, Kyoung-Jae Won, Paul Zhiping Wang, Wendy Osei-Bonsu, Rebecca A. Simmons
Summary: Islet function is critical for maintaining glucose homeostasis, with fetal and neonatal islets showing higher proliferation but decreased insulin secretion in response to stimuli. The study integrated transcriptomes and genome-wide histone landscapes to identify genes and pathways involved in islet development, highlighting the importance of interactions between different cell types in the islets. Epigenetic regulation, particularly histone modifications, was found to play a role in controlling islet development and maturation, with critical transcription factor-binding motifs enriched in histone marks associated with genes essential for islet function.
Article
Endocrinology & Metabolism
Yu-Chin Lien, Xueqing Maggie Lu, Kyoung-Jae Won, Paul Zhiping Wang, Wendy Osei-Bonsu, Rebecca A. Simmons
Summary: The critical pathways for mature islet function include nutrient sensing, neuronal function, immune function, cell replication, and extracellular matrix. The study found that histone marks were enriched at critical transcription factor-binding motifs, such as Hoxa9, C/EBP-beta, Gata1, Foxo1, E2f1, E2f3, and Mafb.
Article
Materials Science, Multidisciplinary
Zhe Gao, Baterdene Namsrai, Zonghu Han, Purva Joshi, Joseph Sushil Rao, Vasanth Ravikumar, Anirudh Sharma, Hattie L. Ring, Djaudat Idiyatullin, Elliott C. Magnuson, Paul A. Iaizzo, Elena G. Tolkacheva, Michael Garwood, Yoed Rabin, Michael Etheridge, Erik B. Finger, John C. Bischof
Summary: This paper explores heart cryopreservation by vitrification-cryogenic storage to extend the preservation of donor hearts, and introduces nanowarming technology to avoid convective rewarming failure, showing potential for organ-level application in hearts. However, further research on physical and biological characteristics is needed.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Review
Immunology
Erinn Zixuan Sim, Nobuaki Shiraki, Shoen Kume
Summary: Recent advancements in differentiating PSCs into pancreatic islet cells have led to the discovery of novel signaling pathways and molecules, with potential therapeutic applications demonstrated through cell transplantation studies. Single-cell transcriptomics and metabolomics have provided valuable insights into endocrine cell growth and pancreatic development.
INFLAMMATION AND REGENERATION
(2021)
Article
Biotechnology & Applied Microbiology
Xiaoyu Yu, Pu Zhang, Yi He, Emily Lin, Huiwang Ai, Melur K. Ramasubramanian, Yong Wang, Yuan Xing, Jose Oberholzer
Summary: Islet beta-cell viability, function, and mass are key attributes for the efficacy of islet transplantation in type 1 diabetes patients. A Smartphone-Fluidic Digital Imaging Analysis (SFDIA) System was developed to quantitatively assess islet mass, showing more accurate and consistent results compared to manual counting methods.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Endocrinology & Metabolism
Sophie Tritschler, Moritz Thomas, Anika Boettcher, Barbara Ludwig, Janine Schmid, Undine Schubert, Elisabeth Kemter, Eckhard Wolf, Heiko Lickert, Fabian J. Theis
Summary: This study provides a high-resolution transcriptional map of healthy human islet cells and their murine and porcine counterparts. The findings suggest both commonalities and differences in transcriptional profiles across different species, with important identity and functional markers shared between species. The pig data better recapitulated the heterogeneity and functional gene expression of human islet cells compared to the mouse data.
MOLECULAR METABOLISM
(2022)