Article
Immunology
Siriyod Denmongkholchai, Keiko Tsuruda, Motoyuki Sugai, Skorn Mongkolsuk, Oranart Matangkasombut
Summary: Study identified host chromatin regulatory genes required for the cytotoxicity of CdtB, with deletions increasing cellular resistance through changes in gene expression profile. Reduced nuclear localization of CdtB in certain mutants may contribute to the mechanisms of CdtB resistance, indicating a potential role of chromatin structure in CDT function.
INFECTION AND IMMUNITY
(2021)
Article
Biochemistry & Molecular Biology
Bruce J. Shenker, Lisa P. Walker, Ali Zekavat, Jonathon Korostoff, Kathleen Boesze-Battaglia
Summary: In this study, the researchers investigated the mechanism of Cdt-induced periodontitis by using cell lines and primary gingival keratinocytes. The findings showed that Cdt caused G2/M arrest in cells and reduced levels of pAkt and pGSK3 beta. Pre-treatment with GSK3 beta kinase inhibitors reversed the G2/M arrest caused by Cdt. Furthermore, Cdt-treated cells displayed increased phosphorylation of CDK1, which was blocked by GSK3 inhibitors and resulted in reduced total CDK1 levels. This study provides further insights into the potential mechanism(s) contributing to Cdt toxicity and toxin-mediated pathogenesis in periodontitis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
En Hyung Chang, Angela C. Brown
Summary: The study found that EGCg increased LtxA production and changed the localization of secreted LtxA from the supernatant to the surface of bacterial cells. In the co-culture model, a single low dose of EGCg did not protect host THP-1 cells from A. actinomycetemcomitans-mediated cytotoxicity, but a multiple dosing strategy had improved effects.
JOURNAL OF PHARMACY AND PHARMACOLOGY
(2021)
Review
Immunology
Yi-Ru Lai, Yu-Fang Chang, Jason Ma, Cheng-Hsun Chiu, Ming-Ling Kuo, Chih-Ho Lai
Summary: Recent studies have shown that bacteria producing CDT target host DNA, potentially contributing to cancer development. Understanding the mechanisms of genotoxins causing DNA damage may help identify potential markers that drive cancer progression and discover new therapeutic strategies to prevent diseases caused by pathogens.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Food Science & Technology
Maryam Keshtvarz, Mahdieh Mahboobi, Marek Kieliszek, Antoni Miecznikowski, Hamid Sedighian, Milad Rezaei, Mohammad Ali Haghighi, Zahra Zareh, Ehsan Rezaei
Summary: The cytolethal distending toxin (CDT) is a bacterial toxin considered for targeted therapies, with its catalytic subunit CdtB capable of inducing DNA double-strand breaks and cell apoptosis. Reduction of CdtB immunogenicity through mutagenesis can enhance stability and enable its use in toxin-based drugs like immunotoxins.
Review
Food Science & Technology
Sung Cheng Looh, Zoey May Pheng Soo, Jia Jia Wong, Hok Chai Yam, Sook Khuan Chow, Jung Shan Hwang
Summary: Leukotoxin A (LtxA) is a major virulence factor of the oral bacterium Aggregatibacter actinomycetemcomitans (Aa) and has been found to be associated with elevated levels of anti-citrullinated protein antibodies (ACPA) in rheumatoid arthritis (RA) patients. LtxA targets leukocytes and triggers an influx of extracellular calcium, leading to hypercitrullination. Although the model-based evidence is yet to be established, the interaction of LtxA with the host immune system has sparked interest in its role in RA.
Article
Immunology
Jiayun Gu, Yan Lin, Zhichao Wang, Qicong Pan, Guohua Cai, Qigai He, Xiaojuan Xu, Xuwang Cai
Summary: This study elucidates the molecular mechanism by which CDT induces pyroptosis via the ROS/caspase-9/caspase-3/GSDME signaling pathway.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Cell Biology
Sarah L. Mathiasen, Laura Gall-Mas, Ioannis S. Pateras, Sofia D. P. Theodorou, Martin R. J. Namini, Morten B. Hansen, Oceane C. B. Martin, Chella Krishna Vadivel, Konstantinos Ntostoglou, Deborah Butter, Michael Givskov, Carsten Geisler, Arne N. Akbar, Vassilis G. Gorgoulis, Teresa Frisan, Niels Odum, Thorbjorn Krejsgaard
Summary: Research shows that the bacterial genotoxin CDT can cause premature senescence in T cells and promote T cell senescence in vivo. Additionally, genotoxin-induced senescent CD4 T cells exhibit a senescence-associated secretory phenotype (SASP), partly orchestrated by the ATMp38 signaling axis.
Article
Biochemistry & Molecular Biology
Miao Qian, Shuyang Cao, Tao Wang, Xiangming Xu, Quan Zhang
Summary: In this study, it was found that bacterial toxin CdtB promotes the expression of inflammatory cytokines, decreases cell viability, and induces cell apoptosis. Moreover, CdtB triggers apoptosis through the MAPK/Erk/p38 signaling pathway and is inhibited by autophagy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Cell Biology
G. Robb Huhn, Naly Torres-Mangual, John Clore, Lucia Cilenti, Teresa Frisan, Ken Teter
Summary: Gram-negative pathogens produce cytolethal distending toxin (CDT) with two cell-binding subunits (CdtA + CdtC) and a catalytic CdtB subunit. After adhesion to the plasma membrane, CDT moves to the endoplasmic reticulum and CdtB enters the nucleus to cause DNA breaks. Our study re-examined the potential endocytosis of CdtA from Haemophilus ducreyi CDT and found rapid degradation of internalised CdtA, CdtB, and CdtC. Internalised CdtA was visualised in early endosomes by confocal microscopy.
CELLULAR MICROBIOLOGY
(2021)
Article
Microbiology
Johannes J. De Soet, Rolf Claesson, Dorte Haubek, Anders Johansson, Mark J. Buijs, Catherine M. C. Volgenant
Summary: In a randomly selected group of inhabitants in Sal, Cape Verde, a minority carry the A. actinomycetemcomitans JP2 genotype, which is not strongly associated with severe periodontitis. Further research is warranted to explore this finding.
Article
Nutrition & Dietetics
Alexandra Adorno Vita, Heather Zwickey, Ryan Bradley
Summary: The study found significant associations between food-specific IgG antibodies and intestinal permeability biomarkers in adults, particularly antibodies against LPS and occludin. While levels of IgG antibodies and permeability biomarkers did not increase in symptomatic patients, the presence of multiple IgG antibodies and increasing titers can be indicative of higher levels of antibodies.
FRONTIERS IN NUTRITION
(2022)
Article
Cell Biology
Jia-Yin Yeh, Hwai-Jeng Lin, Chia-Jung Kuo, Chun-Lung Feng, Chia-Huei Chou, Chia-Der Lin, Hui-Yu Wu, Chen-Yi Li, Cheng-Hsun Chiu, Chih-Ho Lai
Summary: Using recombinant CdtC can alleviate the effects of H. pylori cytotoxins in cells, mitigating H. pylori-induced gastric epithelial cell inflammation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Microbiology
Peyman Kelk, Nick Sina Moghbel, Josefine Hirschfeld, Anders Johansson
Summary: LtxA induces inflammatory cell death involving NLRP3 inflammasome activation and cell-to-cell communication. LtxA stimulates differentiation towards osteoclast-like cells in LPS-treated THP-1 cells.
Article
Immunology
Rolf Claesson, Anders Johansson, Carola Hoeglund Aberg, Anders Esberg, Dorte Haubek, Jan Oscarsson
Summary: The study genetically characterized non-JP2 genotype strains of Aggregatibacter actinomycetemcomitans serotype b isolated from individuals in Ghana and Sweden using multilocus sequence typing (MLST). The results showed a geographic dissemination of non-JP2 genotype strains, and suggested that PCR with cagE-specific primers is an efficient diagnostic procedure for identifying carriers of AP-PCR type 1 strains.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Engineering, Biomedical
Ana Carolina Costa da Mota, Marcela Leticia Leal Goncalves, Anna Carolina Ratto Tempestini Horliana, Alessandro Melo Deana, Lisyanne Araujo de Souza Cavalcante, Andrea Oliver Gomes, Marcia Pinto Alves Mayer, Ellen Sayuri Ando Suguimoto, Kristianne Porta Santos Fernandes, Raquel Agnelli Mesquita-Ferrari, Renato Araujo Prates, Lara Jansiski Motta, Sandra Kalil Bussadori
Summary: The study found that aPDT using a red LED and 0.005% methylene blue resulted in an immediate reduction in halitosis, but the effect was not maintained after 7, 14, or 30 days. No reduction occurred in the number of bacteria investigated at the microbiological level.
LASERS IN MEDICAL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Gabriela de Morais Gouvea Lima, Aline Chiodi Borges, Thalita Mayumi Castaldelli Nishime, Gabriela de Fatima Santana-Melo, Konstantin Georgiev Kostov, Marcia Pinto Alves Mayer, Cristiane Yumi Koga-Ito
Summary: Plasma could be used as an adjuvant therapy for periodontitis, inhibiting Porphyromonas gingivalis growth and potentially improving periodontal tissue recovery in vivo.
Article
Pharmacology & Pharmacy
Natali Shimabukuro, Amalia C. de S. Cataruci, Karin H. Ishikawa, Bruna E. de Oliveira, Dione Kawamoto, Ellen S. Ando-Suguimoto, Emmanuel Albuquerque-Souza, Jacques R. Nicoli, Caroline M. Ferreira, Jean de Lima, Manuela R. Bueno, Leandro B. R. da Silva, Pedro H. F. Silva, Michel R. Messora, Niels O. S. Camara, Maria Regina L. Simionato, Marcia P. A. Mayer
Summary: Bifidobacterium can control periodontitis by modulating immune response and bone destruction, with B. bifidum 162(2A) being more effective than B. breve 110(1A). Both strains showed different effects in the mouse model, with B. bifidum 162(2A) demonstrating potential in controlling periodontitis.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Glauber Campos Vale, Brenda Izabela Santana Mota, Ellen Sayuri Ando-Suguimoto, Marcia Pinto Alves Mayer
Summary: This study evaluated the effect of probiotic lactobacilli on the gene transcription of antibacterial response in monocytes. The results showed that Lactobacillus acidophilus LA-5 promoted the production of inflammatory factors and enhanced the antibacterial activity of innate defense in periodontal tissues, but it may also increase inflammatory response.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Microbiology
Manuela R. Bueno, Karin H. Ishikawa, Gislane Almeida-Santos, Ellen S. Ando-Suguimoto, Natali Shimabukuro, Dione Kawamoto, Marcia P. A. Mayer
Summary: Probiotics may help achieve a balanced microbiome in periodontitis by interfering with the colonization of Aggregatibacter actinomycetemcomitans in the oral cavity. This in vitro study evaluated the effect of two commercially available lactobacilli strains on gingival epithelial cells infected with A. actinomycetemcomitans. The results suggest that these probiotics may reduce the ability of A. actinomycetemcomitans to interact with gingival epithelial cells and modulate the cells' response.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Medicinal
Bruno Bueno-Silva, Manuela Rocha Bueno, Dione Kawamoto, Renato C. Casarin, Joao Marcos Spessoto Pingueiro, Severino Matias Alencar, Pedro Luiz Rosalen, Marcia Pinto Alves Mayer
Summary: (3S)-vestitol, derived from red propolis, exhibits anti-inflammatory, antimicrobial, and anti-caries activity. This study explores the anti-inflammatory mechanisms of (3S)-vestitol on macrophages and reveals its potential as a candidate for the treatment/prevention of persistent inflammatory diseases such as atherosclerosis and periodontitis, involving the inhibition of cytokines and the NF-kappa B pathway.
Editorial Material
Immunology
Marcela Hernandez, Marcia Pinto Alves Mayer, Julien Santi-Rocca
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Gabriela de Morais Gouvea Lima, Celina Faig Lima Carta, Aline Chiodi Borges, Thalita Mayumi Castaldelli Nishime, Cezar Augusto Villela da Silva, Marcelo Vidigal Caliari, Marcia Pinto Alves Mayer, Konstantin Georgiev Kostov, Cristiane Yumi Koga-Ito
Summary: The study evaluated the effects of helium cold atmospheric pressure plasma jet on oral cells, specifically its inhibitory effect on P. gingivalis biofilm and its genotoxicity on epithelial gingival cells and oral fibroblasts.
APPLIED SCIENCES-BASEL
(2022)
Article
Nutrition & Dietetics
Priscila Larcher Longo, Rita de Cassia de Aquino, Sandra Regina Mota Ortiz, Roque Santos de Oliveira, Aline Gavioli, Jonatas Bussador do Amaral, Fernanda Rodrigues Monteiro, Raquel Riyuzo de Almeida Franco, Giovana Rebonatti Mereu, Andre Luis Lacerda Bachi, Alexandre Jose Bancher de Lima, Gilberto Candido Laurentino, Marta Ferreira Bastos
Summary: Physical distancing measures during the COVID-19 pandemic had negative impacts on the health of older women in Brazil, leading to increased BMI, reduced physical activity level, and unhealthy eating habits. The changes in diet and gut microbiota are likely contributors to these effects.
FRONTIERS IN NUTRITION
(2022)
Article
Biology
Ana C. C. Pereira, Alana P. S. Aguiar, Leticia M. P. Araujo, Larissa O. Dantas, Marcia P. A. Mayer, Lamprini Karygianni, Thomas Thurnheer, Ericka T. Pinheiro
Summary: This study found that the combination of antibiofilm agents LL-37 and D-amino acids with antibiotics can have some antimicrobial activity. However, compared to complex biofilms, the combination of antibiotics and D-amino acids did not exhibit significant activity.
Article
Biotechnology & Applied Microbiology
Glauber Campos Vale, Brenda Izabela Santana Mota, Ellen Sayuri Ando-Suguimoto, Marcia Pinto Alves Mayer
Summary: This study aimed to evaluate the antibacterial gene expression of dendritic cells challenged with LPS and probiotics. The results showed that LPS upregulated more genes related to inflammation and immune response compared to the groups where probiotics were added. Although probiotics decreased several genes upregulated by LPS, they still upregulated some genes encoding cytokines. Additionally, one specific probiotic promoted the secretion of these proteins.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Andre L. G. Moreira, Pedro H. F. Silva, Sergio L. Salvador, Karin H. Ishikawa, Graziele C. Ferreira, Jose E. Tanus-Santos, Marcia P. A. Mayer, Sergio L. S. de Souza, Flavia A. C. Furlaneto, Michel R. Messora
Summary: This study evaluated the systemic and local impact of probiotic therapy on rats with metabolic syndrome and periodontitis. The results showed that probiotics can reduce the severity of periodontitis, and modulate lipogenic genes and intestinal morphological and microbiological parameters in rats with metabolic syndrome.
JOURNAL OF PERIODONTOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Andre L. G. Moreira, Giselle A. Silva, Pedro H. F. Silva, Sergio L. Salvador, Raphael M. Vicente, Graziele C. Ferreira, Jose E. Tanus-Santos, Marcia P. A. Mayer, Karin H. Ishikawa, Sergio Luis Scombatti de Souza, Flavia A. C. Furlaneto, Michel R. Messora
Summary: This study found that Bifidobacterium animalis subspecies lactis HN019 can reduce the sequelae of experimental periodontitis in rats by modulating systemic parameters. Bifidobacterium lactis HN019 can decrease dyslipidemia, insulin resistance, proteinuria levels, systolic blood pressure, hepatic steatosis, and expression of adipokines, as well as improve intestinal microbiota imbalance and prevent alveolar bone loss. These findings highlight the importance of Bifidobacterium lactis HN019 in preventing the damage caused by periodontitis.
JOURNAL OF PERIODONTAL RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Mateus Kawata Salgaco, Fellipe Lopes de Oliveira, Adilson Sartoratto, Victoria Mesa, Marcia Pinto Alves Mayer, Katia Sivieri
Summary: Type 2 diabetes is associated with dysbiosis in the gut, which can cause inflammation throughout the body. Probiotic use, specifically Lactobacillus acidophilus-La5, was shown to positively impact the gut microbiome of individuals with type 2 diabetes, leading to potential improvements in glycemic control.
FERMENTATION-BASEL
(2023)
Article
Multidisciplinary Sciences
Carolina Martins Nascimento, Mateus Campos Casaro, Evelyn Roxana Perez, Willian Rodrigues Ribeiro, Marcia Pinto Alves Mayer, Karin Hitomi Ishikawa, Adriana Lino-dos-Santos-Franco, Joice Naiara Bertaglia Pereira, Caroline Marcantonio Ferreira
Summary: Our study investigated the influence of OVA-induced allergic airway inflammation on gut homeostasis, and found that challenges in the respiratory system not only resulted in allergic airway inflammation but also in the loss of gut homeostasis.
