Article
Biochemistry & Molecular Biology
Kurni Kurniyati, Yunjie Chang, Jun Liu, Chunhao Li
Summary: This study investigates the role of multiple flagellin proteins using Treponema denticola as a model. The flagellar filaments of T. denticola are mainly composed of four proteins, regulated by different transcription factors. Each flagellin protein contributes to spirochete motility, and FlaA forms a seam-like structure. These findings demonstrate the unique regulation and assembly mechanism of flagellar filament in T. denticola.
MOLECULAR MICROBIOLOGY
(2022)
Article
Microbiology
Kurni Kurniyati, Yunjie Chang, Jun Liu, Chunhao Li
Summary: This study identifies and characterizes a FliA homolog (TDE2683) in the oral spirochete Treponema denticola and demonstrates its role as a sigma(28) transcription factor regulating class 3 gene expression and flagellar symmetry.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Microbiology
Jana Kacirova, Miriam Sondorova, Aladar Madari, Eva Stykova, Rastislav Mucha, Radomira Nemcova, Nikola Marecakova, Jana Farbakova, Marian Madar
Summary: This study identified periodontal disease-related bacteria in dental plaque of dogs. P. gulae, T. forsythia, and T. denticola were commonly found in dogs with periodontal disease. T. putidum was positively correlated with P. gulae and T. forsythia, suggesting its involvement in periodontal disease development.
Article
Immunology
Erin Trent Malone, Sean Ganther, Nevina Mena, Allan Radaic, Keemia Shariati, Abigail Kindberg, Christian Tafolla, Pachiyappan Kamarajan, J. Christopher Fenno, Ling Zhan, Yvonne L. Kapila
Summary: The periodontal complex, composed of the PDL, alveolar bone, and cementum, plays a key role in maintaining the homeostatic environment of the oral cavity. Dysbiosis of oral microbes may lead to periodontal disease, as seen in the case of Treponema denticola inducing pathological responses in PDL cells through novel mechanisms.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Christopher D. Johnston, M. Paula Goetting-Minesky, Kelly Kennedy, Valentina Godovikova, Sara M. Zayed, Richard J. Roberts, J. Christopher Fenno
Summary: Oral spirochetes, including Treponema, play a crucial role in periodontal disease and oral squamous cell carcinoma. T. denticola is a key model organism for studying spirochete metabolic processes, microbe interactions, and host responses in oral diseases. The use of SyngenicDNA-based strategies and tools enables further investigations into T. denticola physiology and behavior.
MOLECULAR ORAL MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Seiya Yamaki, Masato Tachibana, Hisae Hachimura, Masao Ogawa, Shinya Kanegae, Hirokazu Amimoto, Takashi Shimizu, Kenta Watanabe, Masahisa Watarai, Akiteru Amimoto
Summary: This study aimed to determine the association between gingivitis and oral spirochetes in young cats and dogs. The results showed that the positive rate of spirochetes in plaque samples was significantly higher in young cats (85.4%) than in young dogs (15.4%). These findings suggest that spirochetes may be associated with the early stages of gingivitis in young cats.
Article
Immunology
Navid Mirmohammadsadegh, Neshaut Mashreghi Mohammadi, Mohsen Amin
Summary: Periodontitis, a burdensome disease for healthcare systems, is closely associated with Alzheimer's disease, cardiovascular disease, and diabetes mellitus. Preventive strategies can delay gum inflammation and related chronic diseases. Compared to surgical treatments, preventive approaches have significant cost-effectiveness and long-term health impact on individuals in different communities. However, there are still major hurdles in developing a periodontal vaccine.
EXPERT REVIEW OF VACCINES
(2022)
Review
Dentistry, Oral Surgery & Medicine
Ruohan Li, Li Xiao, Tao Gong, Jiaxin Liu, Yuqing Li, Xuedong Zhou, Yi Li, Xin Zheng
Summary: This article explores the relationship between the oral microbiome and the development of oral cancer, analyzing the role of oral microbiota in inflammation, tumor proliferation, and metastasis. These findings are of great significance for the early diagnosis and treatment of oral cancer.
MOLECULAR ORAL MICROBIOLOGY
(2023)
Article
Microbiology
Huihui Zeng, Yuki Chan, Wenling Gao, W. Keung Leung, Rory M. Watt
Summary: The distribution of different subtypes of oral treponemes in subgingival plaque varies, with periodontitis patients showing a higher diversity of type 2 treponemes compared to gingivitis patients. Patients with periodontitis and gingivitis commonly harbor highly taxonomically diverse communities of oral treponemes.
MICROBIOLOGY SPECTRUM
(2021)
Article
Multidisciplinary Sciences
Ryosuke Ishiwata, Masatomo Iwasa
Summary: Experimental studies have shown that chemotactic cells have cellular memory, with a tendency to maintain migration direction despite changes in the chemoattractant gradient. A phenomenological model analyzing cellular inertia and response time in motility was used to replicate cellular memory observed in experiments. The agreement between experimental data and theory indicates the potential presence of response time in motility, which warrants further exploration.
SCIENTIFIC REPORTS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Dong-Heon Baek, Sung-Hoon Lee
Summary: The study aimed to examine the diverse bioactivity of lipooligosaccharide extracted from T. denticola cultured in the presence of hemin and quorum-sensing inhibitor. Lipooligosaccharide from T. denticola cultured in higher concentration of hemin and 2(5 H)furanone had a greater inhibitory effect on induction of cytokine expression by T. forsythia lipopolysaccharide. Lipooligosaccharide inhibited the binding of T. forsythia lipopolysaccharide to HGFs in the presence of CD14 and LBP.
ARCHIVES OF ORAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xinyi Su, Zhiqun Tang, Zhiyue Lu, Yuqiu Liu, Wanzhi He, Jiapei Jiang, Yifan Zhang, Hongkun Wu
Summary: The study indicates that Treponema denticola can enter the brain and directly affect nerve cells, resulting in the accumulation of A beta. Inhibitors such as KMI1303 and DAPT can suppress the effect of T. denticola on A beta.
JOURNAL OF MOLECULAR NEUROSCIENCE
(2021)
Article
Immunology
Annie N. Hinson, Colin G. Hawkes, Christopher S. Blake, Zackary R. Fitzsimonds, Bin Zhu, Gregory Buck, Richard J. Lamont, Daniel P. Miller
Summary: The oral epithelial barrier serves as both a physical barrier against oral microbiome and a defender for the immune system. The dysbiosis of oral microbiome, including the increase of periodontal pathogens, such as T. denticola, is associated with the progression of periodontitis. The interaction between T. denticola and gingival epithelium induces the elevated expression of IL-36 gamma, which may play a critical role in inducing and amplifying gingival inflammation and subsequent alveolar bone loss.
INFECTION AND IMMUNITY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Yuri Miyai-Murai, Kazuko Okamoto-Shibayama, Toru Sato, Yuichiro Kikuchi, Eitoyo Kokubu, Jan Potempa, Kazuyuki Ishihara
Summary: The Msp protein complex and the serine protease dentilisin are the best-characterized virulence factors in Treponema denticola, the major etiological agent of chronic periodontitis. In addition, the cysteine protease dentipain also contributes to pathogenicity, but its secretion, processing, cellular localization, and role in T. denticola virulence are not fully understood. This study found that dentipain is located in the outer sheath derived from T. denticola dentilisin- and Msp-deficient mutants, suggesting that dentilisin and Msp are involved in the secretion and maturation of dentipain. Inactivation of the dentipain gene slowed the growth of T. denticola, and the mutant strain showed altered gene expression, increased hydrophobicity, reduced autoaggregation activity, and decreased adhesion to IgG.
MOLECULAR ORAL MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Peter Nguyen, Rony Eshaque, Barbara Anne Garland, Anthony Dang, Michael D. L. Suits
Summary: Advanced periodontitis is strongly associated with the presence of the bacterial consortia known as the red complex, which includes Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola. In this study, the researchers investigated the genetic linkage and carbohydrate degradation capacity of T. forsythia, and identified a new polysaccharide utilization loci (PUL)-like operon. They also reported the crystal structures of two proteins encoded in this operon and demonstrated the enzymatic kinetics and degradation process of chondroitin sulfate A by these proteins.
