Article
Biochemistry & Molecular Biology
Marziyeh Babazadeh, Mozhdeh Zamani, Parvaneh Mehrbod, Pooneh Mokarram
Summary: This study investigated the effects of bovine alpha-lactalbumin made lethal to tumor cells and 5-Flourouracil on the stemness of colorectal cancer cells. The results showed that BAMLET and 5-Flourouracil treatment led to decreased expression levels of stemness-related genes and surface markers, with BAMLET being more effective than 5-Flourouracil in inhibiting stemness.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Prabhu Thirusangu, Upasana Ray, Sayantani Sarkar Bhattacharya, Derek B. Oien, Ling Jin, Julie Staub, Nagarajan Kannan, Julian R. Molina, Viji Shridhar
Summary: PFKFB3 is an important enzyme in glycolysis that is overexpressed in many human cancers. Its high expression promotes glycolysis and survival in cancer stem cells, while inhibition of PFKFB3 can reduce proliferation, spheroid formation, chemotherapy resistance, and invasion/migration in SCLC cells.
Article
Chemistry, Medicinal
Chun-Kai Chang, Pei-Fang Chiu, Hui-Yi Yang, Yu-Pu Juang, Yen-Hsun Lai, Tzung-Sheng Lin, Lih-Ching Hsu, Linda Chia-Hui Yu, Pi-Hui Liang
Summary: The study demonstrates that a novel GLUTs-targeting drug conjugate shows better antitumor efficacy than 5-FU in an orthotopic colorectal cancer mice model, with lower exposure of 5-FU during treatment and no significant side effects. This suggests that GLUT-targeting theranostics with a disulfide linker may be a promising approach for antitumor therapy.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Cell Biology
Guangyu Ji, Wenjuan Zhou, Jingyi Du, Juan Zhou, Dong Wu, Man Zhao, Liping Yang, Aijun Hao
Summary: The study identified PCGF1 as a crucial epigenetic regulator in sustaining the stem cell-like phenotype of CRC, with increased PCGF1 expression correlated with cancer progression and poor prognosis. Knockdown of PCGF1 inhibited CRC stem cell proliferation and enrichment, ultimately impairing tumor growth in vivo. Mechanistically, PCGF1 activates transcription of CRC stem cell markers by altering histone trimethylation marks on their promoters.
CELL DEATH & DISEASE
(2021)
Article
Oncology
Lanqing Wang, Lei Zhao, Zhenyu Lin, Dandan Yu, Min Jin, Pengfei Zhou, Jinghua Ren, Jing Cheng, Kunyu Yang, Gang Wu, Tao Zhang, Dejun Zhang
Summary: This study reveals that DCLK1 promotes 5-fluorouracil resistance in colorectal cancer through the CCAR1/beta-catenin pathway-mediated cancer stemness. The results suggest that targeting DCLK1 might be a promising approach to overcome 5-fluorouracil resistance in colorectal cancer by eliminating cancer stem cells.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Cell Biology
Wei Zeng, Jin-Feng Zhu, Jian Guo, Gen-Jie Huang, Li-Sha Ai, Yu Zeng, Wang-Jun Liao
Summary: This study found that circFNDC3B plays a tumor-suppressing role in CRC by inhibiting stemness and metastasis through ASB6 degradation. Regulation of ASB6 and RNF41 has crucial impact on survival and metastasis in CRC.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Ninon Very, Stephan Hardiville, Amelie Decourcelle, Julien Thevenet, Madjid Djouina, Adeline Page, Gerard Vergoten, Celine Schulz, Julie Kerr-Conte, Tony Lefebvre, Vanessa Dehennaut, Ikram El Yazidi-Belkoura
Summary: Alteration of O-GlcNAcylation affects tumorigenesis and tumor progression, with a potential role in chemotherapy response. Combination treatment of 5-FU with Thiamet-G shows synergistic inhibitory effects on CRC progression by regulating TS and O-GlcNacylation levels. Overall, the findings suggest a new approach to enhance CRC response to 5-FU chemotherapy by targeting O-GlcNAcylation.
Article
Cell Biology
Yuanyuan Shang, Zhe Zhu, Yuanyuan Zhang, Fang Ji, Lian Zhu, Mengcheng Liu, Yewei Deng, Guifen Lv, Dan Li, Zhuqing Zhou, Bing Lu, Chuan-gang Fu
Summary: Resistance to radiotherapy is a major challenge in the treatment of locally advanced rectal cancer. This study investigated the role of a specific microRNA, miR-7-5p, in regulating radioresistance and cancer stem cell (CSC) properties in colorectal cancer (CRC). The findings showed that low miR-7-5p expression was associated with poor prognosis in CRC patients and that overexpression of miR-7-5p could reverse radioresistance and attenuate CSC properties in radioresistant CRC cells. The study also demonstrated that miR-7-5p targeted the stemness-associated transcription factor KLF4 and that delivery of miR-7-5p mimics using magnetic nanoparticles inhibited tumor growth in a mouse model of radioresistant CRC. These findings suggest that miR-7-5p could be a potential target for overcoming resistance to radiotherapy in CRC patients.
CELL DEATH DISCOVERY
(2023)
Review
Cell Biology
Huarong Chen, Yifei Wang, Hao Su, Xiaoting Zhang, Hongyan Chen, Jun Yu
Summary: N-6-Methyladenosine (m(6)A) is a common modification on eukaryote messenger RNA, playing a crucial role in posttranscriptional regulation. Recent studies have shown that perturbation of m(6)A regulators can greatly affect cell fate transitions and is associated with various diseases, including cancer.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
V. Nieddu, V. Melocchi, C. Battistini, G. Franciosa, M. Lupia, C. Stellato, G. Bertalot, J. V. Olsen, N. Colombo, F. Bianchi, U. Cavallaro
Summary: Ovarian cancer (OC) has the highest mortality rate among gynecological tumors, primarily due to early peritoneal dissemination, tumor relapse, and chemoresistance. These events are initiated and sustained by ovarian cancer stem cells (OCSC) with self-renewing and tumor-initiating properties. Interfering with OCSC function could offer novel therapeutic perspectives.
CELL DEATH & DISEASE
(2023)
Review
Oncology
Nasim Ebrahimi, Maral Afshinpour, Siavash Seifollahy Fakhr, Paniz Ghasempour Kalkhoran, Vida Shadman-Manesh, Samaneh Adelian, Sheida Beiranvand, Fatemeh Rezaei-Tazangi, Roya Khorram, Michael R. Hamblin, Amir Reza Aref
Summary: Colorectal cancer (CRC) is a major cause of cancer death worldwide. The presence of cancer stem cells (CSCs) contributes to therapy resistance and recurrence. This review examines the role of signaling pathways in colorectal CSCs and discusses potential drugs for treatment of resistant CRC.
CRITICAL REVIEWS IN ONCOLOGY HEMATOLOGY
(2023)
Article
Multidisciplinary Sciences
Hye In Cho, Sora Jo, Min Seong Kim, Han Byeol Kim, Xingzhe Liu, Yanhua Xuan, Jin Won Cho, Yeun Kyu Jang
Summary: Aberrant SETD5 overexpression induces stemness in colorectal cancer cells by upregulating genes related to the PI3K-AKT pathway and CSC markers. SETD5 is functionally related to Pol II and OGT, where OGT-catalyzed Pol II glycosylation depends on SETD5. SETD5 deficiency reduces Pol II occupancy at gene promoters and nullifies the SETD5-induced stemness.
SCIENTIFIC REPORTS
(2023)
Article
Immunology
Martina Musella, Andrea Guarracino, Nicoletta Manduca, Claudia Galassi, Eliana Ruggiero, Alessia Potenza, Ester Maccafeo, Gwenola Manic, Luca Mattiello, Sara Soliman Abdel Rehim, Michele Signore, Marco Pietrosanto, Manuela Helmer-Citterich, Matteo Pallocca, Maurizio Fanciulli, Tiziana Bruno, Francesca De Nicola, Giacomo Corleone, Anna Di Benedetto, Cristiana Ercolani, Edoardo Pescarmona, Laura Pizzuti, Francesco Guidi, Francesca Sperati, Sara Vitale, Daniele Macchia, Massimo Spada, Giovanna Schiavoni, Fabrizio Mattei, Adele De Ninno, Luca Businaro, Valeria Lucarini, Laura Bracci, Eleonora Arico, Giovanna Ziccheddu, Francesco Facchiano, Stefania Rossi, Massimo Sanchez, Alessandra Boe, Mauro Biffoni, Ruggero De Maria, Ilio Vitale, Antonella Sistigu
Summary: The study demonstrates that type I interferons can promote the formation of cancer stem cells by upregulating the chromatin remodeling factor KDM1B. Inhibition of KDM1B could potentially prevent stem cell expansion and increase the long-term benefit of therapy.
Article
Biochemistry & Molecular Biology
Ye-Lim Park, Hwang-Phill Kim, Chan-Young Ock, Dong-Wook Min, Jun Kyu Kang, Yoo Joo Lim, Sang-Hyun Song, Sae-Won Han, Tae-You Kim
Summary: The emergence of RAS/RAF mutant clone is the main feature of EGFR inhibitor resistance in KRAS wild-type colon cancer. In this study, researchers successfully isolated cetuximab-resistant (CR) clonality cells from an in vitro system and found that these cells showed a higher EMT signature and increased expression of CXCL1/5. The study also revealed that CXCL1/5 expression in CR cells was regulated by SNAI2/NFKB and transactivated EGFR through CXCR/MMPI/EGF axis via autocrine signaling.
Article
Cell Biology
Meng-Ling Ye, Si-Qi Li, Yi-Xin Yin, Ke-Zhi Li, Ji-Lin Li, Bang-Li Hu
Summary: Cancer stem cells play crucial roles in colorectal cancer tumorigenesis and treatment response. This study found that the mRNA stemness index (mRNAsi) was associated with the clinical outcome in colorectal cancer (CRC) patients. A stemness-related classification based on mRNAsi scores and RNA sequence data was introduced and showed promise as a prognostic predictor for CRC patients.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)