Article
Cell Biology
Kiranjit K. Bains, Robert D. Young, Elena Koudouna, Philip N. Lewis, Andrew J. Quantock
Summary: Imaging techniques were used to study the three-dimensional microanatomy and spatiotemporal relationships of cells, extracellular matrix, and vasculature at the corneo-scleral limbus of developing and mature chicken eyes. Results showed the development of structures similar to Palisades of Vogt, associated with characteristics of stem cells, and direct cell-cell associations between corneal epithelial and stromal cells.
Article
Nanoscience & Nanotechnology
Johannes A. Oesterreicher, Clemens Simson, Alexander Grossalber, Simon Frank, Stefan Gneiger
Summary: The study demonstrates the spatially resolved quantification of lithium using backscattered electron microscopy in combination with a standard EDS sensor. Good accuracy with minimal deviations is achieved for Mg-Li alloys containing different percentages of lithium, providing a new method for quantitative analysis of lithium in electron microscopes.
SCRIPTA MATERIALIA
(2021)
Article
Materials Science, Multidisciplinary
B. Flipon, V Grand, B. Murgas, A. Gaillac, A. Nicolay, N. Bozzolo, M. Bernacki
Summary: This paper details an image analysis procedure for estimating grain size distribution from optical and scanning electron micrographs, discussing the definition of grain size, classical methodologies, and the impact of data processing parameters on results. The study compares results obtained through electron backscattered diffraction analysis and image analysis based on backscattered electron or optical micrographs, showing the latter to be more efficient in obtaining accurate grain size distribution information.
MATERIALS CHARACTERIZATION
(2021)
Article
Chemistry, Physical
Kelvin Elphick, Akinobu Yamaguchi, Akira Otsuki, Neil Lonio Hayagan, Atsufumi Hirohata
Summary: Our non-destructive imaging technique was successfully used to characterize nanoparticles synthesized by X-ray radiolysis and the sol-gel method, revealing the interfacial conditions and adhesion dependence on solution pH. The results supported the prediction based on the DLVO theory, showing the potential of extending the technique to study different types of interfaces.
Article
Multidisciplinary Sciences
J. C. Stinville, J. M. Hestroffer, M. A. Charpagne, A. T. Polonsky, M. P. Echlin, C. J. Torbet, V. Valle, K. E. Nygren, M. P. Miller, O. Klaas, A. Loghin, I. J. Beyerlein, T. M. Pollock
Summary: The development of high-fidelity mechanical property prediction models relies on large volumes of microstructural feature data. However, spatially correlated measurements of 3D microstructure and deformation fields have been rare. This study presents a unique multi-modal dataset that combines state-of-the-art experimental techniques for 3D tomography and high-resolution deformation field measurements.
Review
Biochemistry & Molecular Biology
Tyler J. Bechtel, Tamara Reyes-Robles, Olugbeminiyi O. Fadeyi, Rob C. Oslund
Summary: Multicellular organisms rely on physical cell-cell interactions to regulate physiological processes. Understanding the complexity of these interactions has greatly improved insight into cell-cell engagement biology and led to opportunities for therapeutic development. Emerging approaches that combine chemistry and biology technologies effectively interrogate cell-cell interfaces with molecular-level detail.
NATURE CHEMICAL BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Tengteng Tang, William Landis, Stephane Blouin, Luca Bertinetti, Markus A. Hartmann, Andrea Berzlanovich, Richard Weinkamer, Wolfgang Wagermaier, Peter Fratzl
Summary: The spatial distribution of mineralization density is vital for bone growth and remodeling processes, while alterations are linked to diseases. In addition to the known lacunocanalicular network, a new nanochannel network has been discovered in the mineralized extracellular matrix of human femoral cortical bone. This network has a greater volume fraction and surface area, potentially allowing for ion and small molecule transport.
JOURNAL OF BONE AND MINERAL RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Mabkhoot Alsaiari, Khumara Roghani, Saad Liaqat, Ali S. Alkorbi, Faiza Sharif, Muhammad Irfan, Moustafa A. Rizk, Maliha Uroos, Naveed Ahmad, Nawshad Muhammad
Summary: This study evaluates the effect of ionic liquids and their structure on the mechanical, mass change, and antifungal properties of soft denture lining material. The addition of ionic liquids improves the surface texture, hardness, and reduces the mass change and tensile strength of the lining material. Ionic liquids also exhibit potent antifungal activity.
Article
Biochemical Research Methods
Tong Xin, Yanan Lv, Haoran Chen, Linlin Li, Lijun Shen, Guangcun Shan, Xi Chen, Hua Han
Summary: This article proposes a new method for serial section registration using an unsupervised optical flow network to measure feature similarity and eliminate nonlinear deformation. The optical flow network is also used to estimate and compensate for cumulative registration error, enabling the reconstruction of biological tissue structures. Experimental results demonstrate that this method effectively improves the spatial continuity of serial sections and enhances the accuracy of registration and reconstruction for biological tissue structures.
Article
Endocrinology & Metabolism
Nadja Fratzl-Zelman, Katherine Wesseling-Perry, Riikka E. Makitie, Stephane Blouin, Markus A. Hartmann, Jochen Zwerina, Ville-Valtteri Valimaki, Christine M. Laine, Matti J. Valimaki, Renata C. Pereira, Outi Makitie
Summary: Patients with WNT1 or PLS3 mutations show unique bone mineralization patterns, with teriparatide having little effect on their bone matrix mineralization or formation. Children exhibit preserved bone formation with heterogeneous mineralization, while adults show low bone turnover with homogenous mineral content.
Article
Engineering, Multidisciplinary
Qiang Zeng, Xiaohu Wang, Rijiao Yang, Nidu Jike, Yu Peng, Jiyang Wang, Ye Tian, Chunsheng Zhou, Shaoqin Ruan, Dongming Yan
Summary: The study aimed to trace imbibition fronts and paths in concrete using transmission microfocus X-ray radiographic tests, with CsCl enhancing X-ray attenuation. Results showed designed experiments enable acquisition of X-ray attenuation images with enhanced contrast, aiding in identifying capillary imbibition paths. Liquid was found to penetrate through the interfacial transition zone and highly porous cement paste more easily.
Article
Microscopy
Matteo Giardino, Devanarayanan Meena Narayana Menon, Davide Luca Janner
Summary: In this study, the possibility of using regularization techniques (Tichonov's and Dirichlet's) for surface reconstruction from FQBSD images in scanning electron microscopy is demonstrated. By reducing distortions caused by sensitivity variations among detector quadrants or imprecise alignment of the FQBSD, these techniques greatly improve the quality of 3D surface reconstruction.
Article
Chemistry, Physical
Anelia Kakanakova-Georgieva, Filippo Giannazzo, Giuseppe Nicotra, Ildiko Cora, Gueorgui K. Gueorguiev, Per O. A. Persson, Bela Pecz
Summary: This study successfully demonstrated the formation of a novel 2D indium oxide, achieved through targeted intercalation and deposition of indium atoms at the graphene/SiC interface. A unique structural configuration with two sub-layers of indium atoms was observed, and bonding of oxygen atoms to indium atoms was confirmed. The 2D indium oxide exhibited a wide bandgap energy of 4.1 eV as estimated by conductive atomic force microscopy measurements.
APPLIED SURFACE SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Diana Banut Onet, Lucian Barbu Tudoran, Ada Gabriela Delean, Petra Surlin, Andreea Ciurea, Alexandra Roman, Sorana D. Bolboaca, Cristina Gasparik, Alexandrina Muntean, Andrada Soanca
Summary: The study evaluated the adhesive capacities of two flowable and two conventional hybrid resin composite materials for non-carious cervical lesions, showing dentinal microleakage despite good peripheral seal. SEM revealed occasional disruptions at the adhesive interface, while EDX confirmed the manufacturers' qualitative compositions. The four experimental composites are recommended for clinical restoration of NCCLs.
APPLIED SCIENCES-BASEL
(2021)
Article
Biotechnology & Applied Microbiology
Yeting Sun, Yue Ma, Hongyang Guan, Hao Liang, Xiaoyan Zhao, Dan Wang
Summary: This study investigates the colonization, proliferation, and biofilm formation process of pathogenic microorganism E. coli O157:H7 in cucumbers. The expression of specific genes and the formation of biofilm in different cucumber tissues at different temperatures were evaluated. The findings provide useful information for the prevention and control of contamination in fresh-cut cucumbers.
Article
Engineering, Biomedical
Andre Schulz, Alisa Katsen-Globa, Esther J. Huber, Sabine C. Mueller, Asger Kreiner, Norbert Puetz, Michael M. Gepp, Benjamin Fischer, Frank Stracke, Hagen von Briesen, Julia C. Neubauer, Heiko Zimmermann
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
(2018)
Article
Multidisciplinary Sciences
Anke Schultz, Anja Germann, Martina Fuss, Marcella Sarzotti-Kelsoe, Daniel A. Ozaki, David C. Montefiori, Heiko Zimmermann, Hagen von Briesen
Article
Engineering, Biomedical
Andre Schulz, Michael M. Gepp, Frank Stracke, Hagen von Briesen, Julia C. Neubauer, Heiko Zimmermann
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2019)
Article
Cell & Tissue Engineering
Johanna Kaindl, Ina Meiser, Julia Majer, Annika Sommer, Florian Krach, Alisa Katsen-Globa, Juergen Winkler, Heiko Zimmermann, Julia C. Neubauer, Beate Winner
STEM CELLS TRANSLATIONAL MEDICINE
(2019)
Article
Computer Science, Information Systems
Carlos Gonzalez-Sanchez, Juan-Carlos Fraile, Javier Perez-Turiel, Ellen Damm, Jochen G. Schneider, Daniel Schmitt, Frank R. Ihmig
Article
Multidisciplinary Sciences
Yannik Muellers, Ina Meiser, Frank Stracke, Iris Riemann, Franziska Lautenschlaeger, Julia C. Neubauer, Heiko Zimmermann
Article
Biology
Asger Kreiner, Frank Stracke, Heiko Zimmermann
Article
Biochemical Research Methods
Simon Memmel, Dmitri Sisario, Heiko Zimmermann, Markus Sauer, Vladimir L. Sukhorukov, Cholpon S. Djuzenova, Michael Flentje
BMC BIOINFORMATICS
(2020)
Article
Automation & Control Systems
Maryam Shariatzadeh, Amit Chandra, Samantha L. Wilson, Mark J. McCall, Lise Morizur, Lea Lesueur, Olivier Chose, Michael M. Gepp, Andre Schulz, Julia C. Neubauer, Heiko Zimmermann, Elsa Abranches, Jennifer Man, Orla O'Shea, Glyn Stacey, Zoe Hewitt, David J. Williams
INTERNATIONAL JOURNAL OF ADVANCED MANUFACTURING TECHNOLOGY
(2020)
Article
Biochemical Research Methods
Ronan Le Harzic, Ina Meiser, Julia C. Neubauer, Iris Riemann, Michael Schiffer, Frank Stracke, Heiko Zimmermann
BIOMEDICAL OPTICS EXPRESS
(2020)
Article
Cell Biology
Gesa Witt, Oliver Keminer, Jennifer Leu, Rashmi Tandon, Ina Meiser, Anne Willing, Ingo Winschel, Jana-Christin Abt, Bjoern Braendl, Isabelle Sebastien, Manuel A. Friese, Franz-Josef Mueller, Julia C. Neubauer, Carsten Claussen, Heiko Zimmermann, Philip Gribbon, Ole Pless
Summary: This study highlights the significance of the embryonic stem cell test (EST) and its potential applications in pharmaceutical development. Several improvements to the workflow have been introduced to enhance sensitivity, speed, and reproducibility, paving the way for predicting in vivo developmental toxicity from in vitro data.
CELL BIOLOGY AND TOXICOLOGY
(2021)
Article
Cell & Tissue Engineering
Rachel Steeg, Julia C. Neubauer, Sabine C. Muller, Andreas Ebneth, Heiko Zimmermann
STEM CELL RESEARCH
(2020)
Article
Cell & Tissue Engineering
Rachel Steeg, Sabine C. Mueller, Nancy Mah, Bjorn Holst, Alfredo Cabrera-Socorro, Glyn N. Stacey, Paul A. De Sousa, Aidan Courtney, Heiko Zimmermann
Summary: The generation of disease-relevant human induced pluripotent stem cells requires adherence to ethical, legal, and quality standards, with best practices integrated into daily operations for maximizing their potential. Key experiences from operating the European Bank for induced Pluripotent Stem Cells (EBiSC) over 7 years are discussed, with recommendations on how to incorporate solutions into daily management framework.
Article
Biology
Ina Meiser, Julia Majer, Alisa Katsen-Globa, Andre Schulz, Katharina Schmidt, Frank Stracke, Eirini Koutsouraki, Gesa Witt, Oliver Keminer, Ole Pless, John Gardner, Carsten Claussen, Philip Gribbon, Julia C. Neubauer, Heiko Zimmermann
Summary: The gold standard in cryopreservation is currently the slow freezing method, but there is a need for more sophisticated cryopreservation protocols, especially for hiPSCs, to meet the increasing demand for therapeutic and diagnostic cell types. A novel vitrification-based method for adherent hiPSCs has been developed, with potential applications in suspension-based bioreactors after thawing. By optimizing conditions such as microcarrier stiffness and adhesion time, droplet-based vitrification was found to be superior to conventional slow freezing in preserving cell characteristics and minimizing cell loss.
Article
Multidisciplinary Sciences
Kai Melde, Heiner Kremer, Minghui Shi, Senne Seneca, Christoph Frey, Ilia Platzman, Christian Degel, Daniel Schmitt, Bernhard Schoelkopf, Peer Fischer
Summary: Acoustic waves can generate forces on matter, allowing for the precise shaping of ultrasound fields in 3D. This technique enables the one-step assembly of particles, hydrogel beads, and biological cells using acoustic forces without the need for opposing waves or support structures. The structures can be fixed by gelation. This advancement holds great potential for tissue engineering and additive manufacturing.
