Hepatoprotective effect of Ficus religiosa latex on cisplatin induced liver injury in Wistar rats

Ficus religiosa L., Moraceae, is widely planted in the tropics. The chemical constituents of F. religiosa include tannin, saponin gluanol acetate, p-sitosterol, leucoanthocyanidin, and leucoanthocyanin. These are used for the treatment of pain, inflammation, impotence, menstrual disturbances, and urine related problems, and as uterine tonic. The present study aimed to evaluate hepatoprotective effects of F. religiosa latex on cisplatin induced liver injury in Wistar rats. In experimental protocol contained five groups of rats (n=6). In which, group I (control) was administered acacia (2%, w/v) of 5 ml/kg throughout the experiment for 16 days. The group II (cisplatin treated) was administered single dose of cisplatin (7.5 mg/kg i.p.) on 1st day. Group III (extract control) was administered 300 mg/kg p.o. of extract for 1st to 10th day. Group IV (Protective) was administered extract (300 mg/kg p.o.) of F. religiosa latex for 1st to 10th day and administered single dose of cisplatin (7.5 mg/kg i.p.) on 11th day and group V (Curative) received single dose of cisplatin (7.5 mg/kg i.p.) on day 1st, and administered extract (300 mg/kg p.o.) from 7th to 16th days. On the 6th day in cisplatin treated, 10th day in extract control and 16th day in control, protective and curative, blood withdrawn from retro-orbital sinus of rats for biochemical estimation for serum and dissected out the livers for estimation of antioxidant enzymes and histopathological works. The cisplatin-treated group 2 showed a significant increase in serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and hepatocytes cells degeneration inflammatory infiltrate and necrosis it's were significantly (**p <0.01) alleviates by protective groups. (C) 2015 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda. All rights reserved.