Article
Biophysics
Zhenzhen Han, Xinyan Peng, Yi Yang, Jia Yi, Dan Zhao, Qiyuan Bao, Shuping Long, Sai-Xi Yu, Xin-Xin Xu, Baohong Liu, Yan-Jun Liu, Yuhui Shen, Liang Qiao
Summary: In this study, a method combining microfluidics and surface-enhanced Raman spectroscopy (SERS) was developed to isolate exosomes from plasma and profile exosomal biomarkers for osteosarcoma diagnosis. The method showed efficient isolation of exosomes directly from human plasma and could profile exosomes based on protein biomarkers with a low detection limit. The analysis of plasma-derived exosomes from osteosarcoma patients and healthy controls revealed significantly higher levels of specific protein biomarkers on exosomes from osteosarcoma patients. A classification model was built based on the exosomal biomarkers, achieving high sensitivity, specificity, and accuracy. This method, which is simple and cost-effective, has great potential for clinical cancer diagnosis as a liquid biopsy technique.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Multidisciplinary Sciences
Zhenglin Li, Chao Liu, Yangchang Cheng, Yike Li, Jinqi Deng, Lixiao Bai, Lili Qin, Huili Mei, Min Zeng, Fei Tian, Shaohua Zhang, Jiashu Sun
Summary: Tumor-derived extracellular vesicles (EVs) have the potential to improve noninvasive early cancer diagnosis. However, the lack of effective methods for isolating and detecting nanosized EVs in blood samples has been a challenge. To address this, researchers designed cascaded microfluidic circuits to filter EVs using a pneumatic clock pulse generator. This device allows for rapid and high-purity isolation of EVs from blood within 30 minutes and has shown 91% accuracy in diagnosing early-stage breast cancer.
Review
Chemistry, Inorganic & Nuclear
Shuai Zhang, Feilong Rong, Chuanpan Guo, Fenghe Duan, Linghao He, Minghua Wang, Zhihong Zhang, Mengmeng Kang, Miao Du
Summary: Metal-organic frameworks (MOFs) have emerged as promising porous crystalline materials with various applications due to their large surface areas, tunable pore scales, and good adsorption capabilities. They have been utilized for developing highly sensitive and selective biosensors, particularly for early cancer diagnosis and treatment monitoring, by anchoring diverse probes for construction of electrochemical biosensors.
COORDINATION CHEMISTRY REVIEWS
(2021)
Review
Chemistry, Multidisciplinary
Xindan Zhang, Xiao Tan, Ping Wang, Jieling Qin
Summary: Although CRC is easy to treat surgically, its five-year survival rate is still poor. Therefore, developing sensitive detection technology is essential for early diagnosis, which can provide more opportunities for effective treatment. Blood biomarkers, especially those detected by electrochemical biosensors, show significant potential for early diagnosis and prognosis of CRC. This review mainly discusses the characteristics and applications of PPy-based biosensors for CRC biomarker detection, as well as the opportunities and challenges associated with their use for diagnosing CRC.
Article
Chemistry, Analytical
Yang Lu, Changbiao Zhan, Liandong Yu, Yiyue Yu, Huakun Jia, Xiaozhe Chen, Dongzhi Zhang, Rongke Gao
Summary: We developed a novel multifunctional nanocone array as a solid immunoassay plate and SERS substrate for the simultaneous detection of PSA and thrombin based on a microfluidic chip. Compared to traditional immunoassays, this nanocone array with a deposited gold film provides a larger surface area for efficient target antigen capture. By integrating this microfluidic chip, we achieved simultaneous detection of PSA and thrombin with high sensitivity. This detection strategy shows great potential for early prostate cancer diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Wei Zhang, Juanrui Du, Kun Wang, Yige Li, Cong Chen, Long Yang, Zitong Kan, Biao Dong, Lin Wang, Lin Xu
Summary: Periodontitis, a prevalent chronic inflammation, has inconspicuous symptoms at early stage, leading to permanent damage. Timely diagnosis and monitoring are crucial for clinical treatment. A dual-channel electrochemical immunosensor was developed for simultaneous detection of periodontitis-related biomarkers in saliva. The sensor showed excellent sensitivity, low limits of detection, and high correlation coefficients, with potential for point-of-care diagnosis and monitoring of periodontitis.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Multidisciplinary
Peilong Li, Jiaci Chen, Yuqing Chen, Shangling Song, Xiaowen Huang, Yang Yang, Yanru Li, Yao Tong, Yan Xie, Juan Li, Shunxiang Li, Jiayi Wang, Kun Qian, Chuanxin Wang, Lutao Du
Summary: In this study, a 3D porous sponge microfluidic chip with an exosome enrichment platform was constructed, achieving a capture efficiency of approximately 90%. A specific exosome membrane protein (SORL1) for colorectal cancer was identified through deep mass spectrometry analysis, and a specific quantum dot labeling method for SORL1 detection was developed. An ensemble classification system was established using features extracted from 64-patched fluorescence images, and it showed a significantly higher area under the curve (AUC) of 0.99 compared to the conventional biomarker carcinoembryonic antigen (CEA) with an AUC of 0.71. This system demonstrated similar diagnostic performance for early-stage CRC, young CRC, and CEA-negative CRC patients.
Article
Biochemical Research Methods
Lihua Ding, Xia Liu, Zhenzhong Zhang, Li-e Liu, Sitian He, Yan Wu, Clement Yaw Effah, Ruiying Yang, Aiai Zhang, Wensi Chen, Mubarak Yarmamat, Lingbo Qu, Xiaonan Yang, Yongjun Wu
Summary: In this study, a microfluidic chip was designed to isolate exosomes from plasma, improving their capture efficiency and purity. The chip was also used for plasma-based cancer diagnosis by monitoring exosomal biomarkers.
Article
Chemistry, Analytical
Ahmed A. Shalaby, Chia-Wen Tsao, Akihiko Ishida, Masatoshi Maeki, Manabu Tokeshi
Summary: Cancer is a leading cause of death worldwide, and early diagnosis is crucial for successful treatment. In developing countries, there is a need for low-cost, accurate, and easy to use point-of-need (PON) devices for cancer diagnosis and treatment follow-up. Paper-based analytical devices (PADs) are considered a key solution as they provide a low-cost platform for developing PON biosensors. This review discusses the various factors involved in the development of PADs for cancer biomarker detection and summarizes the advantages and disadvantages of each method.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Review
Engineering, Biomedical
Xueli Yuan, Bowen Lin, Tao Liu, Wei Zhang, Zhenyu Chu, Xiaoping Gu, Zhengliang Ma, Wanqin Jin
Summary: Cancer is a malignant disease with a high fatality rate, and early screening with rapid and accurate detection is crucial for improving the survival rate. Electrochemical biosensors that detect cancer biomarkers in blood offer advantages such as low invasiveness, fast diagnosis, and low cost. However, there is a trade-off between sensitivity and selectivity, which limits the detection of trace amounts of biomarkers. Recently, nanomaterials with improved sensitivity and selectivity have been developed to address this issue. This review introduces different categories of state-of-the-art electrochemical biosensors, discusses their operating principles and advantages/disadvantages, and evaluates the existing detection strategies and performance of nanomaterial-based cancer biosensors.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Biochemical Research Methods
Shibo Cheng, Yutao Li, He Yan, Yunjie Wen, Xin Zhou, Lee Friedman, Yong Zeng
Summary: Extracellular vesicles, including exosomes, play a significant role in cancer diagnosis and therapeutic monitoring by carrying a selective set of biomolecules from tumor cells. Microfluidics offers a disruptive platform for EV isolation and analysis due to its advantages in promoting the development of new molecular and cellular sensing systems with improved sensitivity and specificity. Despite current challenges in EV research, adapting microfluidic techniques shows promise in assessing emerging EV-associated biomarkers for clinical applications.
Article
Neurosciences
Yareth Gopar-Cuevas, Ana P. Duarte-Jurado, Rosa N. Diaz-Perez, Odila Saucedo-Cardenas, Maria J. Loera-Arias, Roberto Montes-de-Oca-Luna, Humberto Rodriguez-Rocha, Aracely Garcia-Garcia
Summary: Parkinson's disease is a leading neurodegenerative movement disorder worldwide, often occurring in idiopathic form. The diagnostic accuracy is relatively low, highlighting the urgent need to establish a panel of biomarkers for early and accurate diagnosis. Accurate diagnosis may lead to unraveling the pathogenesis and ultimately finding a cure.
MOLECULAR NEUROBIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Cristina Herrera-Pariente, Sheyla Montori, Joan Llach, Alex Bofill, Eduardo Albeniz, Leticia Moreira
Summary: Gastric cancer is a common cancer worldwide, with early detection being crucial for improving clinical outcomes. Current screening programs are effective in high-incidence populations but not cost-effective in low-incidence populations, highlighting the need for new noninvasive biomarkers.
Article
Multidisciplinary Sciences
Hyunku Shin, Byeong Hyeon Choi, On Shim, Jihee Kim, Yong Park, Suk Ki Cho, Hyun Koo Kim, Yeonho Choi
Summary: This study successfully detected the presence of 6 types of early-stage cancer and classified the tumor organ type using artificial intelligence and surface-enhanced Raman spectroscopy. It shows potential in early cancer detection and has the ability to expand to other diseases.
NATURE COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Zodwa Dlamini, Mzwandile Mbele, Tshepiso J. Makhafola, Rodney Hull, Rahaba Marima
Summary: Globally, HIV/AIDS and cancer are significant public health problems, with sub-Saharan Africa bearing the largest burden. While AIDS-defining cancers are decreasing, non-AIDS defining cancers are on the rise in HIV-positive individuals.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Yunsheng Chen, Yixia Zhang, Fei Pan, Jie Liu, Kan Wang, Chunlei Zhang, Shangli Cheng, Lungen Lu, Wei Zhang, Zheng Zhang, Xiao Zhi, Qian Zhang, Gabriel Alfranca, Jesus M. de la Fuente, Di Chen, Daxiang Cui
Article
Chemistry, Multidisciplinary
Chunchun Meng, Xiao Zhi, Chao Li, Chuanfeng Li, Zongyan Chen, Xusheng Qiu, Chan Ding, Lijun Ma, Hongmin Lu, Di Chen, Guangqing Liu, Daxiang Cui
Article
Chemistry, Multidisciplinary
Jin Wu, Kai Tao, Yuanyuan Guo, Zhong Li, Xiaotian Wang, Zhongzhen Luo, Shuanglong Feng, Chunlei Du, Di Chen, Jianmin Miao, Leslie K. Norford
Article
Engineering, Electrical & Electronic
Shujing Lin, Junkai Xu, Xiao Zhi, Di Chen, Jianmin Miao, Peter Bradley Shull, Daxiang Cui
IEEE ELECTRON DEVICE LETTERS
(2017)
Article
Engineering, Electrical & Electronic
Yihuan Shen, Di Chen, Qian Wei, Shujing Lin, Liyun Shi, Linsheng Wu, Ge Guo
IEEE ELECTRON DEVICE LETTERS
(2018)
Article
Biophysics
Shujing Lin, Xiao Zhi, Di Chen, Fangfang Xia, Yihuan Shen, Jiaqi Niu, Shiyi Huang, Jie Song, Jianmin Miao, Daxiang Cui, Xianting Ding
BIOSENSORS & BIOELECTRONICS
(2019)
Review
Chemistry, Multidisciplinary
Shujing Lin, Zixian Yu, Di Chen, Zhigang Wang, Jianmin Miao, Qichao Li, Daoyuan Zhang, Jie Song, Daxiang Cui
Article
Nanoscience & Nanotechnology
Qichao Li, Di Chen, Jianmin Miao, Shujing Lin, Zixian Yu, Yutong Han, Zhi Yang, Xiao Zhi, Daxiang Cui, Zhenghua An
ACS APPLIED MATERIALS & INTERFACES
(2020)
Article
Chemistry, Analytical
Qichao Li, Di Chen, Jianmin Miao, Shujing Lin, Zixian Yu, Daxiang Cui, Zhi Yang, Xianping Chen
Summary: A high-performance gas sensor based on ordered porous ZnO nanosheets with fast response and high sensitivity for ethanol detection applications is presented in this study.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Engineering, Electrical & Electronic
Qichao Li, Yamin Liu, Di Chen, Jianmin Miao, Shujing Lin, Daxiang Cui
Summary: Flexible piezoresistive sensors are prepared using L-cystine and aerogel, showing good linearity and great potential for applications in electronic skin.
IEEE ELECTRON DEVICE LETTERS
(2021)
Article
Chemistry, Analytical
Qichao Li, Yamin Liu, Di Chen, Jianmin Miao, Xiao Zhi, Shengwei Deng, Shujing Lin, Han Jin, Daxiang Cui
Summary: Silver doping significantly impacts the sensing properties of graphene-based sensors, with silver-doped graphene showing the highest sensitivity to NO2, which enhances the gas sensor's ability to track NO2 effectively.
Article
Biophysics
Zixian Yu, Shujing Lin, Fangfang Xia, Yanlei Liu, Di Zhang, Fei Wang, Yanpu Wang, Qichao Li, Jiaqi Niu, Chengxi Cao, Daxiang Cui, Nengquan Sheng, Jiazi Ren, Zhigang Wang, Di Chen
Summary: The ExoSD chip is a highly integrated platform for exosome separation and detection, capable of efficiently separating and detecting gastric cancer cell-derived exosomes with high recovery and purity.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Engineering, Electrical & Electronic
Shujing Lin, Di Zhang, Yanpu Wang, Zixian Yu, Qichao Li, Fei Wang, Yuanchao Li, Yiping Guo, Jianmin Miao, Daxiang Cui, Di Chen
Summary: This study presents a cost-effective and solderable fabrication process for manufacturing stretchable circuit boards, demonstrating their excellent solderability. The feasibility of these circuit boards for wearable embedded systems is shown through the development of soft wireless pressure monitoring prototypes and soft posture detection and control systems. Tests reveal that the circuit boards and embedded systems are robust enough for application in wearable systems.
SENSORS AND ACTUATORS A-PHYSICAL
(2021)
Article
Nanoscience & Nanotechnology
Qichao Li, Yamin Liu, Di Chen, Jianmin Miao, Chunlei Zhang, Daxiang Cui
Summary: In this study, wearable strain sensors with good sensitivity, stretchability, softness, durability, and biocompatibility were prepared using soft silicone elastomer and carbon nanotubes. The sensors exhibited high sensitivity and a large sensing strain range, as well as strong robustness and biocompatibility.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Review
Biochemical Research Methods
Fei Wang, Shujing Lin, Zixian Yu, Yanpu Wang, Di Zhang, Chengxi Cao, Zhigang Wang, Daxiang Cui, Di Chen
Summary: This review discusses the applications of electroporation in biotechnology, providing an overview of its mechanism, principles, and detection methods. It also presents the recent advances in microfluidic-based electroporation devices and discusses the challenges and outlook for cell membrane electroporation.
